公开(公告)号：US11726078B2

公开(公告)日：2023-08-15

申请号：US17091501

申请日：2020-11-06

Applicant: General Electric Company ,  Tokitae LLC

Inventor： Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Cathryn Ellen Olsen ,  John Richard Nelson ,  David Roger Moore ,  Paul Michael Smigelski, Jr. ,  John Thomas Connelly ,  Benjamin David Grant ,  Bernhard Hans Weigl

IPC: G01N33/493 ,  C07K1/22 ,  B01D15/08 ,  G01N33/50 ,  G01N33/569 ,  B01D63/02 ,  A61B10/00 ,  C12M1/26 ,  G01N33/558 ,  G01N15/14

CPC classification number: G01N33/493 ,  A61B10/007 ,  B01D15/08 ,  B01D63/02 ,  C07K1/22 ,  C12M33/14 ,  G01N33/5038 ,  G01N33/558 ,  G01N33/569 ,  G01N33/5695 ,  A61M2202/005 ,  A61M2202/0028 ,  A61M2202/0071 ,  A61M2202/0496 ,  G01N2015/1493

Abstract: A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.

公开(公告)号：US11714084B2

公开(公告)日：2023-08-01

申请号：US17090383

申请日：2020-11-05

Applicant: General Electric Company

Inventor： Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Cathryn Ellen Olsen ,  John Richard Nelson ,  Brian Christopher Bales ,  David Roger Moore ,  Paul Michael Smigelski, Jr.

IPC: G01N33/569 ,  G01N1/40 ,  G01N33/543 ,  B01L3/00 ,  B01D15/36

CPC classification number: G01N33/5695 ,  B01D15/363 ,  B01L3/5023 ,  G01N1/4005 ,  G01N33/54366 ,  B01L2200/0631 ,  B01L2200/10 ,  B01L2300/0825 ,  G01N2001/4011 ,  G01N2333/35 ,  G01N2405/06 ,  G01N2469/10

Abstract: A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.

公开(公告)号：US20210338805A1

公开(公告)日：2021-11-04

申请号：US17244642

申请日：2021-04-29

Applicant: General Electric Company

Inventor： Christopher Michael Puleo ,  Victoria Eugenia Cotero ,  John Richard Nelson

IPC: A61K39/215 ,  C12N15/86 ,  C12N13/00 ,  C07K14/54

Abstract: The subject matter of the present disclosure generally relates to techniques for addressing or correcting dysregulation of the trauma regulation pathway. The dysregulation may be associated with a physiological condition, such as a SARS-CoV-2 viral infection. In an embodiment, the techniques include treating dysregulation based on a renin-angiotensin pathway molecule or cell and/or a splenic pathway molecule or cell using targeted neuromodulation. In an embodiment, neuromodulation is used to regulate the immune system, e.g., as an energy-based adjuvant for a vaccine.

公开(公告)号：US20180305718A1

公开(公告)日：2018-10-25

申请号：US15491125

申请日：2017-04-19

Applicant: General Electric Company

Inventor： John Richard Nelson ,  Robert Scott Duthie ,  Patrick McCoy Spooner ,  John Anthony Schiel ,  Lisa Anne Lowery ,  Anja Josifa Smith

IPC: C12N15/90 ,  C12N15/11

CPC classification number: C12N15/907 ,  C12N15/102 ,  C12N15/11 ,  C12N2310/20

Abstract: A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5′ flanking sequence and a donor 3′ flanking sequence. The donor 5′ flanking sequence and the donor 3′ flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.

公开(公告)号：US10077459B2

公开(公告)日：2018-09-18

申请号：US15145838

申请日：2016-05-04

Applicant: General Electric Company

Inventor： John Richard Nelson ,  Robert Scott Duthie ,  Erik Leeming Kvam ,  Wei Gao

IPC: C12P21/00 ,  C12N15/63 ,  C12N15/67

CPC classification number: C12P21/00 ,  C12N15/63 ,  C12N15/67 ,  C12Q2531/125

Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.

公开(公告)号：US09920359B2

公开(公告)日：2018-03-20

申请号：US15177149

申请日：2016-06-08

Applicant: General Electric Company

Inventor： Ryan Charles Heller ,  John Richard Nelson

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/04 ,  C12N9/00

CPC classification number: C12Q1/6853 ,  C12P19/34 ,  C12Q1/6865 ,  C12Q2521/301 ,  C12Q2523/31 ,  C12Q2525/179 ,  C12Q2531/119 ,  C12Q2531/125 ,  C12Q2531/143

Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use an oligocation-oligonucleotide conjugate primer for amplifying a target nucleic acid to generate amplicons. Isothermal DNA amplification methods that employ a strand displacing DNA polymerase and polyamine-oligonucleotide conjugate primer are also provided.

公开(公告)号：US20170321239A1

公开(公告)日：2017-11-09

申请号：US15145838

申请日：2016-05-04

Applicant: General Electric Company

Inventor： John Richard Nelson ,  Robert Scott Duthie ,  Erik Leeming Kvam ,  Wei Gao

IPC: C12P21/00

CPC classification number: C12P21/00 ,  C12N15/63 ,  C12N15/67 ,  C12Q2531/125

Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.

公开(公告)号：US20160289735A1

公开(公告)日：2016-10-06

申请号：US14672358

申请日：2015-03-30

Applicant: General Electric Company

Inventor： Michael John Gerdes ,  John Richard Nelson ,  Patrick McCoy Spooner ,  Ralf Lenigk

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6804 ,  C12N15/1003 ,  C12Q2522/101 ,  G01N33/689

Abstract: A method is provided herein, wherein the method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample, comprises contacting a lysis solution to the sample comprising at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate comprises a protamine-DNA complex. The method further comprises applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The antibody binding is followed by capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex.

Abstract translation: 本文提供了一种方法，其中在样品中捕获精子脱氧核糖核酸（DNA）的方法包括将裂解液与包含至少精子细胞或精细胞裂解物的样品接触以裂解精子细胞。 精子细胞或精子细胞裂解物包含鱼精蛋白-DNA复合物。 该方法还包括向裂解的精子细胞施加至少一种鱼精蛋白特异性抗体，其中鱼精蛋白特异性抗体结合裂解的精细胞的鱼精蛋白-DNA复合物以形成抗体 - 鱼精蛋白-DNA复合物。 抗体结合后，捕获抗体 - 鱼精蛋白-DNA复合物; 并从捕获的抗体 - 鱼精蛋白-DNA复合物中分离和检测精子DNA。

公开(公告)号：US09063041B2

公开(公告)日：2015-06-23

申请号：US13690801

申请日：2012-11-30

Applicant: General Electric Company

Inventor： Christopher Michael Puleo ,  John Richard Nelson ,  Patrick McCoy Spooner ,  Ralf Lenigk

IPC: B01L3/00 ,  B01L7/00 ,  G01N1/30 ,  G01N1/40

CPC classification number: G01N1/30 ,  A01N1/0242 ,  A01N1/0284 ,  B01L3/5023 ,  B01L3/5088 ,  B01L7/00 ,  B01L2200/0678 ,  B01L2300/0825 ,  B01L2300/126 ,  B01L2300/1877 ,  G01N2001/4027 ,  Y10T436/2525

Abstract: A method of drying a biological sample disposed on a substrate is provided. The method comprises providing the substrate comprising a sample loading area and a heat source; activating the heat source for generating heat; heating the substrate at least above 65° C.; and drying the biological sample. A device for storing sample is also provided, wherein the device comprises a substrate for biological sample-storage; and a heating component that generates heat to maintain a temperature of at least above 65° C. The heating component may contain one or more reagents, wherein the reagents generate heat to maintain a temperature of at least above 65° C.

Abstract translation: 提供了一种干燥设置在基板上的生物样品的方法。 该方法包括提供包括样品加载区域和热源的基底; 激活热源产生热量; 将衬底加热至少高于65℃; 并干燥生物样品。 还提供了用于存储样品的装置，其中所述装置包括用于生物样品储存的基底; 以及产生热量以保持至少高于65℃的温度的加热组分。加热组分可以含有一种或多种试剂，其中试剂产生热量以保持至少高于65℃的温度。

公开(公告)号：US20150031035A1

公开(公告)日：2015-01-29

申请号：US13952173

申请日：2013-07-26

Applicant: General Electric Company

Inventor： Erik Leeming Kvam ,  John Richard Nelson ,  Gregory Andrew Grossmann ,  Ryan Charles Heller ,  Erin Jean Finehout ,  Christopher Michael Puleo ,  William Patrick Waters

IPC: C12Q1/68

CPC classification number: C12N15/1017 ,  B01L7/52 ,  B01L2300/0681 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6806 ,  C12Q2521/501 ,  C12Q2531/125

Abstract: Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-cellular fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.

Abstract translation: 本文提供了从生物样品的非细胞部分收集和扩增循环核酸的方法。 从非细胞级分提取循环核酸，并进行环化以产生单链核酸环，然后通过使用随机引物的滚动循环扩增产生扩增文库，然后扩增其。 还提供了用于从生物样品中收集非细胞级分的装置。 该装置包括与过滤膜直接接触的过滤膜和干燥固体基质。