公开(公告)号：US20190187142A1

公开(公告)日：2019-06-20

申请号：US15848301

申请日：2017-12-20

Applicant: General Electric Company

Inventor： Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Cathryn Ellen Olsen ,  John Richard Nelson ,  Brian Christopher Bales ,  David Roger Moore ,  Paul Michael Smigelski, JR.

IPC: G01N33/569 ,  G01N1/40 ,  G01N33/543 ,  B01L3/00 ,  B01D15/36

CPC classification number: G01N33/5695 ,  B01D15/363 ,  B01L3/5023 ,  B01L2200/0631 ,  B01L2200/10 ,  G01N1/4005 ,  G01N33/54366 ,  G01N2001/4011 ,  G01N2333/35 ,  G01N2405/06 ,  G01N2469/10

Abstract: A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.

公开(公告)号：US09719082B2

公开(公告)日：2017-08-01

申请号：US14068532

申请日：2013-10-31

Applicant: General Electric Company

Inventor： Bing Li ,  Gregory Andrew Grossmann ,  David Roger Moore

IPC: C07H21/00 ,  C12N15/10

CPC classification number: C12N15/1006

Abstract: A solid substrate for biological sample storage under dry-state and elution of biomolecules is provided. The dry, solid substrate comprises a surface modified with a plurality of hydrophilic groups; and the substrate is comprised of one or more protein denaturing agents impregnated therein under a substantially dry state. A method for elution of biomolecules from biological samples is also provided. The compositions disclosed herein provide for enhanced elution and recovery of biomolecules, such as nucleic acids, from the sample. The sample is disposed on a substrate, dried to a substantially dry state; eluted from the biological sample dried on the substrate by rehydrating the substrate in an elution buffer.

公开(公告)号：US20170212112A1

公开(公告)日：2017-07-27

申请号：US15005054

申请日：2016-01-25

Applicant: General Electric Company

Inventor： Bing Li ,  Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Cathryn Ellen Olsen ,  Arunkumar Natarajan

IPC: G01N33/569

CPC classification number: G01N33/56905 ,  G01N33/54393 ,  G01N2333/445

Abstract: Provided herein are integrated devices that improve sensitivity of rapid diagnostic tests. The devices described herein integrate sample separation with rapid diagnostic tests and improve signal intensity of the rapid diagnostic tests.

公开(公告)号：US20160002621A1

公开(公告)日：2016-01-07

申请号：US14321160

申请日：2014-07-01

Applicant: General Electric Company

Inventor： John Richard Nelson ,  David Roger Moore ,  Robert Scott Duthie ,  Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Elizabeth Marie Dees ,  Patrick McCoy Spooner ,  Erik Leeming Kvam ,  Andrew Arthur Paul Burns ,  Vicki Herzl Watkins

IPC: C12N15/10

CPC classification number: C12N15/1006 ,  C12Q1/6834 ,  C12Q2565/625

Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.

Abstract translation: 本文提供了一种方法，所述方法包括：将包含靶核酸的样品施加到底物的样品施用区; 并使核酸扩增反应混合物通过样品施用区跨越一定长度的底物扩增形成核酸扩增产物的靶核酸; 其中具有第一分子量的靶核酸基本上固定在样品施用区，并且其中具有第二分子量的扩增产物从样品施用区迁移。 还提供了相关联的设备。

公开(公告)号：US20210055297A1

公开(公告)日：2021-02-25

申请号：US17090383

申请日：2020-11-05

Applicant: General Electric Company

Inventor： Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Cathryn Ellen Olsen ,  John Richard Nelson ,  Brian Christopher Bales ,  David Roger Moore ,  Paul Michael Smigelski

IPC: G01N33/569 ,  G01N1/40 ,  G01N33/543 ,  B01L3/00 ,  B01D15/36

Abstract: A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.

公开(公告)号：US10830760B2

公开(公告)日：2020-11-10

申请号：US15848302

申请日：2017-12-20

Applicant: General Electric Company ,  TOKITAE LLC

Inventor： Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Cathryn Ellen Olsen ,  John Richard Nelson ,  David Roger Moore ,  Paul Michael Smigelski, Jr. ,  John Thomas Connelly ,  Benjamin David Grant ,  Bernhard Hans Weigl

IPC: G01N33/493 ,  C07K1/22 ,  B01D15/08 ,  G01N33/50 ,  G01N33/569 ,  B01D63/02 ,  A61B10/00 ,  C12M1/26 ,  G01N33/558 ,  G01N15/14

Abstract: A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.

公开(公告)号：US20180001231A1

公开(公告)日：2018-01-04

申请号：US15209411

申请日：2016-07-13

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Christopher Michael Puleo ,  Craig Patrick Galligan ,  Gregory Andrew Grossmann ,  Erik Leeming Kvam ,  Robert Scott Duthie ,  Kenneth Wayne Rigby ,  Paul Michael Smigelski, JR. ,  Victoria Eugenia Cotero ,  Jason William Castle ,  John Donald Burczak ,  James Edward Rothman

IPC: B01D21/00 ,  G01N1/40 ,  G01N33/49 ,  B01L3/00

Abstract: A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.

公开(公告)号：US20210055282A1

公开(公告)日：2021-02-25

申请号：US17091501

申请日：2020-11-06

Applicant: General Electric Company ,  Tokitae LLC

Inventor： Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Cathryn Ellen Olsen ,  John Richard Nelson ,  David Roger Moore ,  Paul Michael Smigelski ,  John Thomas Connelly ,  Benjamin David Grant ,  Bernhard Hans Weigl

IPC: G01N33/493 ,  C07K1/22 ,  B01D15/08 ,  G01N33/50 ,  G01N33/569 ,  B01D63/02 ,  A61B10/00 ,  C12M1/26 ,  G01N33/558

Abstract: A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.

公开(公告)号：US10787695B2

公开(公告)日：2020-09-29

申请号：US15611586

申请日：2017-06-01

Applicant: General Electric Company

Inventor： Christopher Michael Puleo ,  Christine Lynne Surrette ,  Erik Leeming Kvam ,  Steven Yuehin Go ,  Feng Chen ,  John Richard Nelson ,  Craig Patrick Galligan ,  Radislav Alexandrovich Potyrailo ,  Gregory Andrew Grossmann

IPC: C12Q1/02 ,  C12M1/34 ,  G01N21/64 ,  C12Q1/18 ,  G01N33/483

Abstract: A system includes a bacteria culture array that includes a plurality of chambers each configured to receive a portion of a sample that includes bacteria. Each individual chamber of the plurality of chambers includes a chamber opening configured to permit access of the portion of the sample to the individual chamber. The system also includes one or more sensors configured to collect data from the individual chamber. The sensors are configured to contact the sample. Additionally, the system includes a monitoring and analysis system that includes a processor configured to receive the data from the one or more sensors at a first time and a second time, compare the data received at the second time to the data received at the first time, and identify a portion of the plurality of chambers of the bacteria culture array based on the comparing.

公开(公告)号：US09644232B2

公开(公告)日：2017-05-09

申请号：US13952173

申请日：2013-07-26

Applicant: General Electric Company

Inventor： Erik Leeming Kvam ,  John Richard Nelson ,  Gregory Andrew Grossmann ,  Ryan Charles Heller ,  Erin Jean Finehout ,  Christopher Michael Puleo ,  William Patrick Waters

IPC: C12Q1/68

CPC classification number: C12N15/1017 ,  B01L7/52 ,  B01L2300/0681 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6806 ,  C12Q2521/501 ,  C12Q2531/125

Abstract: Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-cellular fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.