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公开(公告)号：US20210002688A1
公开(公告)日：2021-01-07
申请号：US16459336
申请日：2019-07-01
Applicant: General Electric Company
Inventor： Alex David Corwin , Erik Leeming Kvam , Christine Lynne Surrette , Brian Joseph Scherer , Pei-Hsin Kuo , Ralf Lenigk
Abstract: The present disclosure relates to approaches for assessing a sample or the presence of microorganisms. The sample, in certain implementations may be assessed for one or both of absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). sample partition device may be employed that partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.
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公开(公告)号：US20200080047A1
公开(公告)日：2020-03-12
申请号：US16128121
申请日：2018-09-11
Applicant: General Electric Company
Inventor： Matthew Jeremiah Misner , Rachel Marie Gettings , Christine Angela Morton , Erik Leeming Kvam , Jason William Castle , Anindya Kanti De , Jared Timothy Hale , Craig Patrick Galligan , Vincent Francis Pizzi
Abstract: A system for isolating a target molecule from a bioprocess fluid includes a single-use disposable separation device having a plurality of perimeter-bonded layers defining one or more mesofluidic channels of the separation device, wherein each layer includes a biocompatible polymer material, wherein the separation device is configured to separate at least a portion of particles from the bioprocess fluid to generate a substantially clarified bioprocess fluid, and a chromatography system fluidically coupled at the outflow of the separation device in a configuration for further processing the clarified bioprocess fluid.
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公开(公告)号：US10450345B2
公开(公告)日：2019-10-22
申请号：US15619148
申请日：2017-06-09
Applicant: General Electric Company
Inventor： Erik Leeming Kvam , Bruce Allan Hay , Ernest William Kovacs
IPC: C07K1/36 , C08L71/02 , C07K16/00 , B01D11/04 , C12N15/11 , B01D17/02 , C07K1/14 , B01J20/26 , B01D15/38 , C12N15/115
Abstract: A method for purification of a target in a solution includes providing a target in a solution, the solution comprising one or more contaminants, providing a polymer conjugate in the solution, wherein the polymer conjugate is configured to specifically bind the target, incubating the solution at a first temperature to facilitate binding of the polymer conjugate to the target, providing an environmentally-responsive block copolymer in the solution, wherein the environmentally-responsive block copolymer comprises one or more of an ethylene oxide (EO), a propylene oxide (PO), or an EO/PO block copolymer, and heating the solution comprising the environmentally-responsive block copolymer and the polymer conjugate bound to the target at a second temperature to initiate a liquid-liquid phase separation, wherein the liquid-liquid phase separation produces an aqueous phase and a liquid polymer phase, and wherein the liquid polymer phase comprises the polymer conjugate bound to the target.
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公开(公告)号：US20180001231A1
公开(公告)日：2018-01-04
申请号：US15209411
申请日：2016-07-13
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Christopher Michael Puleo , Craig Patrick Galligan , Gregory Andrew Grossmann , Erik Leeming Kvam , Robert Scott Duthie , Kenneth Wayne Rigby , Paul Michael Smigelski, JR. , Victoria Eugenia Cotero , Jason William Castle , John Donald Burczak , James Edward Rothman
Abstract: A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.
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5.发明授权Substrates and methods for collection, stabilization and elution of biomolecules 有权用于收集，稳定和洗脱生物分子的底物和方法公开(公告)号：US09480966B2
公开(公告)日：2016-11-01
申请号：US14261900
申请日：2014-04-25
Applicant: General Electric Company
Inventor： Ernest William Kovacs , Erik Leeming Kvam , Bing Li , Frank John Mondello
CPC classification number: B01J20/28038 , B01J20/22 , B01J20/24 , B01J20/28033 , B01J20/3212 , B01J20/3242 , B01J20/3246 , C07K1/145 , C07K1/16 , C07K14/525 , C07K14/5421 , C07K14/545 , C07K14/775 , C12N9/2471 , C12N15/1006 , C12N15/1017 , C12Y302/01023
Abstract: A solid substrate for the extraction, stabilization, and storage of proteins is provided. The substrate includes: a polysaccharide, such as melezitose under a substantially dry state. The substrate is configured to extract proteins from a sample and stabilize the extracted proteins in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the proteins stored in the dry solid substrate are also described.
Abstract translation: 提供了用于提取，稳定和储存蛋白质的固体底物。 底物包括：多糖，例如基本干燥状态下的松三草。 底物被配置为从样品中提取蛋白质并在环境条件下以干燥形式稳定所提取的蛋白质较长时间。 还描述了收集和回收储存在干固体基质中的蛋白质的方法。
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Patent Agency Ranking
公开(公告)号：US20230219080A1
公开(公告)日：2023-07-13
申请号：US17573311
申请日：2022-01-11
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Ralf Lenigk , Tejas Suresh Khire , Tyler Hammond , Erik Leeming Kvam , Allison Caron
IPC: B01L3/00
CPC classification number: B01L3/5021 , B01L3/502715 , B01L2300/087 , B01L2200/10
Abstract: A closed system centrifuge tube is described and discussed herein that enables removal of waste and transfer and removal of samples via one or more ports of the centrifuge tube within the closed system, without exposing the sample to the outer environment. This may enable greater sample retention during sample processing and centrifugation of the sample.
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公开(公告)号：US10077459B2
公开(公告)日：2018-09-18
申请号：US15145838
申请日：2016-05-04
Applicant: General Electric Company
Inventor： John Richard Nelson , Robert Scott Duthie , Erik Leeming Kvam , Wei Gao
CPC classification number: C12P21/00 , C12N15/63 , C12N15/67 , C12Q2531/125
Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
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公开(公告)号：US20170321239A1
公开(公告)日：2017-11-09
申请号：US15145838
申请日：2016-05-04
Applicant: General Electric Company
Inventor： John Richard Nelson , Robert Scott Duthie , Erik Leeming Kvam , Wei Gao
IPC: C12P21/00
CPC classification number: C12P21/00 , C12N15/63 , C12N15/67 , C12Q2531/125
Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
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公开(公告)号：US09534214B2
公开(公告)日：2017-01-03
申请号：US14068633
申请日：2013-10-31
Applicant: General Electric Company
Inventor： Bing Li , Gregory Andrew Grossmann , Erik Leeming Kvam , Brian Christopher Bales , Jason Louis Davis
CPC classification number: C12N15/1006 , B01J20/22 , B01J20/261 , B01J20/321 , B01J20/3212 , B01J20/3248 , B01J20/3251 , B01J20/3255 , B01J20/3274 , C12N15/1017
Abstract: A solid substrate for biological sample storage under dry-state and elution of biomolecules is provided. The dry, solid substrate is coated with saccharides, such as monosaccharides, oligosaccharides, polysaccharides or combinations thereof, and the substrate is comprised of one or more protein denaturing agents impregnated therein under a substantially dry state. A method for elution of biomolecules from biological samples is also provided. The compositions disclosed herein provide for enhanced elution and recovery of biomolecules, such as nucleic acids, from the sample. The sample is disposed on a substrate, dried to a substantially dry state; eluted from the biological sample dried on the substrate by rehydrating the substrate in an elution buffer.
Abstract translation: 提供了一种在干态下生物样品储存和生物分子洗脱的固体底物。 干燥的固体底物涂覆有糖，例如单糖，寡糖，多糖或其组合，并且底物由一种或多种在基本干燥状态下浸渍的蛋白质变性剂组成。 还提供了用于从生物样品中洗脱生物分子的方法。 本文公开的组合物提供来自样品的生物分子（例如核酸）的增强的洗脱和回收。 将样品置于基材上，干燥至基本干燥状态; 通过在洗脱缓冲液中再水化底物，从在底物上干燥的生物样品中洗脱。
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10.发明授权公开(公告)号：US09347033B2
公开(公告)日：2016-05-24
申请号：US14813406
申请日：2015-07-30
Applicant: General Electric Company
Inventor： Zaeem Ashraf Khan , Aaron Joseph Dulgar-Tulloch , Stefan Rakuff , Philip Alexander Shoemaker , Erik Leeming Kvam , Xiaohui Chen , Jaydeep Roy
CPC classification number: C12M45/09 , B01F5/0057 , B01F5/0206 , B01F5/0231 , B01F5/0256 , B01F5/10 , C12M27/00 , C12M47/04 , C12N5/0653 , C12N2501/70 , C12N2501/734 , C12N2521/00
Abstract: A method of processing an adipose tissue to collect adipose derived regenerative cells is provided, wherein the method comprises providing a vessel comprising a fluid jet mixer, introducing the adipose tissue into the vessel, introducing a buffer solution into the vessel; washing the adipose tissue using the fluid jet mixer; introducing an enzyme solution into the vessel; initiating jet mixing into the vessel comprising the adipose tissue, the enzyme solution, and the buffer solution using the fluid jet mixer to digest the adipose tissue to form a digestion product; phase-separating the digestion product into a digested buoyant fat layer and a non-buoyant aqueous layer; and collecting the non-buoyant aqueous layer comprising the adipose derived regenerative cells. A system of processing an adipose tissue to collect adipose derived regenerative cells is also provided.
Abstract translation: 提供了一种处理脂肪组织以收集脂肪来源的再生细胞的方法，其中所述方法包括提供包含流体喷射混合器的容器，将脂肪组织引入容器中，将缓冲溶液引入容器中; 使用流体喷射混合器洗涤脂肪组织; 将酶溶液引入容器中; 使用流体喷射混合器将包含脂肪组织，酶溶液和缓冲溶液的射流混合到消化脂肪组织以形成消化产物; 将消化产物相分离成消化的浮力脂肪层和非浮力水层; 并收集包含脂肪来源的再生细胞的非浮力水层。 还提供了一种处理脂肪组织以收集脂肪来源的再生细胞的系统。
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