公开(公告)号：US20210055297A1

公开(公告)日：2021-02-25

申请号：US17090383

申请日：2020-11-05

Applicant: General Electric Company

Inventor： Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Cathryn Ellen Olsen ,  John Richard Nelson ,  Brian Christopher Bales ,  David Roger Moore ,  Paul Michael Smigelski

IPC: G01N33/569 ,  G01N1/40 ,  G01N33/543 ,  B01L3/00 ,  B01D15/36

Abstract: A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.

公开(公告)号：US20160146795A1

公开(公告)日：2016-05-26

申请号：US14548338

申请日：2014-11-20

Applicant: General Electric Company

Inventor： Bing Li ,  Brian Christopher Bales ,  David Roger Moore ,  Jason Michael Nichols ,  Cathryn Ellen Olsen ,  William Christopher Alberts ,  Frank John Mondello

IPC: G01N33/548

CPC classification number: G01N33/548 ,  B01D67/009 ,  B01D69/144 ,  B01D71/78 ,  G01N33/54353 ,  Y02A50/57

Abstract: A modified porous membrane comprising a polymer coating grafted to a porous membrane is described. A method for analyte detection from a biological sample using an immunoassay is also provided using the modified membrane, wherein the method comprises a) providing a modified porous membrane having the structure of Formula (I), b) incubating the modified porous membrane with a first biomolecule, wherein the first biomolecule binds to the modified porous membrane to form a first biomolecule bound modified porous membrane; and c) adding a biological sample comprising at least an analyte to the first biomolecule bound modified porous membrane for analyte detection by binding the analyte to the first biomolecule bound to the modified porous membrane.

Abstract translation: 描述了包含接枝到多孔膜上的聚合物涂层的改性多孔膜。 还提供了使用免疫测定法从生物样品中分析物检测的方法，其中所述方法包括：a）提供具有式（I）结构的改性多孔膜，b）将所述改性多孔膜与第一 生物分子，其中所述第一生物分子与修饰的多孔膜结合以形成第一生物分子结合的改性多孔膜; 和c）将包含至少一种分析物的生物样品添加到第一生物分子结合的改性多孔膜上，以通过将分析物结合到与修饰的多孔膜结合的第一生物分子来进行分析物检测。

公开(公告)号：US20160146802A1

公开(公告)日：2016-05-26

申请号：US14548383

申请日：2014-11-20

Applicant: General Electric Company

Inventor： Bing Li ,  Brian Christopher Bales ,  David Roger Moore ,  Jason Michael Nichols ,  Cathryn Ellen Olsen ,  William Christopher Alberts ,  Frank John Mondello

IPC: G01N33/543 ,  G01N33/548 ,  G01N33/558

CPC classification number: G01N33/54386 ,  G01N33/54353 ,  G01N33/548 ,  G01N33/558

Abstract: A device comprising a modified porous membrane is provided. The modified porous membrane comprises a polymer coating grafted to a porous membrane. The device is used for analyte detection from a biological sample using an immunoassay. The device comprises a sample application zone at one end of the device for applying a biological sample comprising a target analyte; and a detection zone present at another end of the device, downstream of the sample application zone for detecting the target analyte, wherein the detection zone comprises one or more first biomolecules immobilized on a modified porous membrane having a structure of Formula (I).

Abstract translation: 提供了包含改性多孔膜的装置。 改性多孔膜包括接枝到多孔膜上的聚合物涂层。 该装置用于使用免疫测定从生物样品进行分析物检测。 该装置包括用于施加包含目标分析物的生物样品的装置的一端处的样品施加区域; 以及位于所述装置的另一端的检测区，其位于所述样品施加区的下游，用于检测所述目标分析物，其中所述检测区包含固定在具有式（I）的结构的改性多孔膜上的一个或多个第一生物分子。

公开(公告)号：US20130289257A1

公开(公告)日：2013-10-31

申请号：US13721948

申请日：2012-12-20

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Brian Christopher Bales ,  Erik Leeming Kvam ,  Jason Louis Davis

IPC: B01J20/04

CPC classification number: B01J20/045 ,  B01J20/24 ,  C12N15/1006 ,  C12N15/1017 ,  G01N1/40

Abstract: The present disclosure generally relates to dry solid matrices for the extraction, stabilization, and storage of nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.

Abstract translation: 本公开内容通常涉及用于在环境条件下长时间提取，稳定和储存核酸，特别是RNA的干固体基质。 还描述了收集和回收存储在干燥固体基质中的核酸的方法。

公开(公告)号：US09399075B2

公开(公告)日：2016-07-26

申请号：US13872539

申请日：2013-04-29

Applicant: General Electric Company

Inventor： Matthew David Butts ,  Robert Edgar Colborn ,  Peter John Bonitatibus, Jr. ,  Andrew Soliz Torres ,  Brian Christopher Bales ,  Michael Ernest Marino ,  Bruce Allan Hay ,  Amit Mohan Kulkarni

IPC: A61K49/04 ,  C01G35/00 ,  A61K49/00 ,  A61K49/18 ,  B82Y5/00 ,  B82Y30/00

CPC classification number: A61K49/101 ,  A61K49/0002 ,  A61K49/04 ,  A61K49/0428 ,  A61K49/0485 ,  A61K49/1818 ,  A61K49/1848 ,  B82Y5/00 ,  B82Y30/00 ,  C01G35/00 ,  Y10S977/773 ,  Y10S977/779 ,  Y10S977/927 ,  Y10S977/928 ,  Y10T428/2982

Abstract: Compositions of nanoparticles functionalized with at least one zwitterionic moiety, methods for making a plurality of nanoparticles, and methods of their use as diagnostic agents are provided. The nanoparticles have characteristics that result in minimal retention of the particles in the body compared to other nanoparticles. The nanoparticle comprising a nanoparticulate transition metal oxide covalently functionalized with a silane-functionalized non-targeting zwitterionic moiety.

Abstract translation: 提供了用至少一个两性离子部分官能化的纳米颗粒的组合物，制备多个纳米颗粒的方法及其用作诊断剂的方法。 与其他纳米颗粒相比，纳米颗粒具有导致颗粒在体内的最小保留的特征。 纳米颗粒包含用硅烷官能化的非靶向两性离子部分共价官能化的纳米颗粒过渡金属氧化物。

公开(公告)号：US20160038617A1

公开(公告)日：2016-02-11

申请号：US14919077

申请日：2015-10-21

Applicant: General Electric Company

Inventor： Brian Christopher Bales ,  Bruce Allan Hay ,  Binil Itty Ipe Kandapallil

IPC: A61K49/18

CPC classification number: A61K49/1857 ,  A61K49/0428 ,  A61K49/1842 ,  A61K49/186 ,  B82Y5/00

Abstract: The present application discloses treating water insoluble nanoparticles, particularly nanoparticles of metals and metal compounds which find utility in diagnostic imaging such as MR and X-ray imaging, with an alpha-hydroxyphosphonic acid conjugate with a hydrophilic moiety to render the nanoparticles sufficiently hydrophilic to find utility in diagnostic imaging. Among the modified hydrophilic nanoparticles disclosed are those in which the hydrophilic moieties of the modifying conjugate are ethylene oxide based polymers and copolymers and zwitterions and the nanoparticles are composed of transition metal oxides such as superparamagnetic iron oxide and tantalum oxide. Disclosed are nanoparticles which are sufficiently hydrophilic to form stable aqueous colloidal suspensions. Also disclosed is diagnostic imaging such as MR and X-ray using the modified hydrophilic nanoparticles as contrast agents.

Abstract translation: 本申请公开了处理水不溶性纳米颗粒，特别是金属和金属化合物的纳米颗粒，其在诊断成像如MR和X射线成像中具有与亲水部分的α-羟基膦酸缀合物的效用，以使纳米颗粒充分亲水以发现 在诊断成像中的应用。 所公开的改性亲水性纳米粒子中的那些是其中改性缀合物的亲水部分是环氧乙烷基聚合物和共聚物和两性离子，并且纳米颗粒由过渡金属氧化物如超顺磁性氧化铁和氧化钽组成。 公开了足够亲水以形成稳定的水性胶体悬浮液的纳米颗粒。 还公开了使用修饰的亲水性纳米颗粒作为造影剂的诊断成像如MR和X射线。

公开(公告)号：US08981069B2

公开(公告)日：2015-03-17

申请号：US13721948

申请日：2012-12-20

Applicant: General Electric Company

Inventor： Brian Christopher Bales ,  Erik Leeming Kvam ,  Jason Louis Davis

IPC: B01J20/04 ,  B01J20/24 ,  C12N15/10 ,  G01N1/40

Abstract: The present disclosure generally relates to dry solid matrices for the extraction, stabilization, and storage of nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.

公开(公告)号：US20190187142A1

公开(公告)日：2019-06-20

申请号：US15848301

申请日：2017-12-20

Applicant: General Electric Company

Inventor： Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Cathryn Ellen Olsen ,  John Richard Nelson ,  Brian Christopher Bales ,  David Roger Moore ,  Paul Michael Smigelski, JR.

IPC: G01N33/569 ,  G01N1/40 ,  G01N33/543 ,  B01L3/00 ,  B01D15/36

CPC classification number: G01N33/5695 ,  B01D15/363 ,  B01L3/5023 ,  B01L2200/0631 ,  B01L2200/10 ,  G01N1/4005 ,  G01N33/54366 ,  G01N2001/4011 ,  G01N2333/35 ,  G01N2405/06 ,  G01N2469/10

Abstract: A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.

公开(公告)号：US09827335B2

公开(公告)日：2017-11-28

申请号：US14919077

申请日：2015-10-21

Applicant: General Electric Company

Inventor： Brian Christopher Bales ,  Bruce Allan Hay ,  Binil Itty Ipe Kandapallil

IPC: A61K49/18 ,  A61K49/04 ,  B82Y5/00

CPC classification number: A61K49/1857 ,  A61K49/0428 ,  A61K49/1842 ,  A61K49/186 ,  B82Y5/00

Abstract: The present application discloses treating water insoluble nanoparticles, particularly nanoparticles of metals and metal compounds which find utility in diagnostic imaging such as MR and X-ray imaging, with an alpha-hydroxyphosphonic acid conjugate with a hydrophilic moiety to render the nanoparticles sufficiently hydrophilic to find utility in diagnostic imaging. Among the modified hydrophilic nanoparticles disclosed are those in which the hydrophilic moieties of the modifying conjugate are ethylene oxide based polymers and copolymers and zwitterions and the nanoparticles are composed of transition metal oxides such as superparamagnetic iron oxide and tantalum oxide. Disclosed are nanoparticles which are sufficiently hydrophilic to form stable aqueous colloidal suspensions. Also disclosed is diagnostic imaging such as MR and X-ray using the modified hydrophilic nanoparticles as contrast agents.

公开(公告)号：US09040679B2

公开(公告)日：2015-05-26

申请号：US13968497

申请日：2013-08-16

Applicant: General Electric Company

Inventor： Erik Leeming Kvam ,  Bing Li ,  Brian Christopher Bales

IPC: C12N15/10 ,  B01J20/24

CPC classification number: B01J20/24 ,  C12N15/1003 ,  C12N15/1006

Abstract: A solid matrix for the extraction, stabilization, and storage of nucleic acids is provided. At least one protein denaturant, and at least one acid or acid-titrated buffer reagent are impregnated in a dry state therein the matrix; and the matrix is configured to provide an acidic pH on hydration. The matrix is configured to extract nucleic acids from a sample and stabilize the extracted nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.

Abstract translation: 提供了用于提取，稳定和储存核酸的固体基质。 至少一种蛋白质变性剂和至少一种酸或酸滴定的缓冲剂在基质中以干燥状态浸渍; 并且所述基质配置成在水合时提供酸性pH。 该基质构造成从样品中提取核酸，并在环境条件下长时间地以干燥形式稳定所提取的核酸，特别是RNA。 还描述了收集和回收存储在干燥固体基质中的核酸的方法。