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公开(公告)号：US10350307B2
公开(公告)日：2019-07-16
申请号：US15707074
申请日：2017-09-18
Applicant: General Electric Company
Inventor： Brian Michael Davis , John Richard Nelson , Wei Gao
Abstract: A method for in vivo RNA or protein expression is provided. The method includes introducing a double-stranded concatemeric DNA into a eukaryotic cell to generate a desired RNA or protein. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences, wherein each of the plurality of tandem repeat sequences comprises an expression sequence. The double-stranded concatemeric DNA comprises one or more phosphorothioated nucleotides, wherein a ratio of phosphorothioated nucleotides to total nucleotides in the double-stranded concatemeric DNA is at least 1:1600. A eukaryotic cell comprising an exogeneous, double-stranded concatemeric DNA comprising the plurality of tandem repeat sequences is also provided.
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2.发明申请公开(公告)号：US20190083655A1
公开(公告)日：2019-03-21
申请号：US15707074
申请日：2017-09-18
Applicant: General Electric Company
Inventor： Brian Michael Davis , John Richard Nelson , Wei Gao
IPC: A61K48/00
CPC classification number: A61K48/0066 , C12N15/63 , C12N15/67 , C12Q2531/125
Abstract: A method for in vivo RNA or protein expression is provided. The method includes introducing a double-stranded concatemeric DNA into a eukaryotic cell to generate a desired RNA or protein. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences, wherein each of the plurality of tandem repeat sequences comprises an expression sequence. The double-stranded concatemeric DNA comprises one or more phosphorothioated nucleotides, wherein a ratio of phosphorothioated nucleotides to total nucleotides in the double-stranded concatemeric DNA is at least 1:1600. A eukaryotic cell comprising an exogeneous, double-stranded concatemeric DNA comprising the plurality of tandem repeat sequences is also provided.
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公开(公告)号：US20190071704A1
公开(公告)日：2019-03-07
申请号：US16041734
申请日：2018-07-20
Applicant: GENERAL ELECTRIC COMPANY
Inventor： John Richard Nelson , Robert Scott Duthie , Erik Leeming Kvam , Wei Gao
Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
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公开(公告)号：US20190003940A1
公开(公告)日：2019-01-03
申请号：US15639511
申请日：2017-06-30
Applicant: General Electric Company , University of Akron
Inventor： Arunkumar Natarajan , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Wei Gao , Kwok Pong Chan , Lakshmi Sireesha Kaanumalle , Abraham Joy , Nicholas Nun
Abstract: A method includes providing a biological sample, providing a sample collection device, wherein the sample collection device includes a sample binding surface including a photodegradable polymer configured to bind the biological sample, contacting the biological sample with the sample binding surface of the sample collection device, and irradiating the sample binding surface and the bound biological sample using light emitted from a light source to initiate degradation of the photodegradable polymer of the sample binding surface to cause release of the biological sample.
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公开(公告)号：US10077459B2
公开(公告)日：2018-09-18
申请号：US15145838
申请日：2016-05-04
Applicant: General Electric Company
Inventor： John Richard Nelson , Robert Scott Duthie , Erik Leeming Kvam , Wei Gao
CPC classification number: C12P21/00 , C12N15/63 , C12N15/67 , C12Q2531/125
Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
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Patent Agency Ranking
公开(公告)号：US20170321239A1
公开(公告)日：2017-11-09
申请号：US15145838
申请日：2016-05-04
Applicant: General Electric Company
Inventor： John Richard Nelson , Robert Scott Duthie , Erik Leeming Kvam , Wei Gao
IPC: C12P21/00
CPC classification number: C12P21/00 , C12N15/63 , C12N15/67 , C12Q2531/125
Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
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公开(公告)号：US09625355B2
公开(公告)日：2017-04-18
申请号：US14557169
申请日：2014-12-01
Applicant: GENERAL ELECTRIC COMPANY
Inventor： John Richard Nelson , Wei Gao , Christopher Michael Puleo , Todd Frederick Miller , Christine Lynne Pitner , David Andrew Shoudy , Alex David Corwin
CPC classification number: C12N15/1013 , B01L3/502 , B01L2200/025 , B01L2200/0689 , B01L2300/06 , B01L2300/0654 , B01L2300/0861 , C12Q1/6806 , G01N1/02 , G01N1/10 , G01N1/40 , G01N2001/028 , G01N2001/4038
Abstract: A system for extracting material from a region of interest includes a fluid delivery base comprising an inlet channel and an outlet channel formed within the fluid delivery base; a gasket affixed to the fluid delivery base, wherein the gasket comprises at least one opening exposing an open end of the inlet channel and an open end of the outlet channel; a support comprising a sample-supporting surface facing the gasket and an opposing surface; and an alignment member coupled to the opposing surface in a fixed position and such that the support is positioned between the fluid delivery base and the alignment member, wherein one or both of the alignment member or the fluid delivery base are biased towards one another by a force (e.g., a magnet or spring force) and wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.
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公开(公告)号：US20160153868A1
公开(公告)日：2016-06-02
申请号：US14557169
申请日：2014-12-01
Applicant: GENERAL ELECTRIC COMPANY
Inventor： John Richard Nelson , Wei Gao , Christopher Michael Puleo , Todd Frederick Miller , Christine Lynne Pitner , David Andrew Shoudy , Alex David Corwin
IPC: G01N1/10
CPC classification number: C12N15/1013 , B01L3/502 , B01L2200/025 , B01L2200/0689 , B01L2300/06 , B01L2300/0654 , B01L2300/0861 , C12Q1/6806 , G01N1/02 , G01N1/10 , G01N1/40 , G01N2001/028 , G01N2001/4038
Abstract: A system for extracting material from a region of interest includes a fluid delivery base comprising an inlet channel and an outlet channel formed within the fluid delivery base; a gasket affixed to the fluid delivery base, wherein the gasket comprises at least one opening exposing an open end of the inlet channel and an open end of the outlet channel; a support comprising a sample-supporting surface facing the gasket and an opposing surface; and an alignment member coupled to the opposing surface in a fixed position and such that the support is positioned between the fluid delivery base and the alignment member, wherein one or both of the alignment member or the fluid delivery base are biased towards one another by a force (e.g., a magnet or spring force) and wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.
Abstract translation: 用于从感兴趣区域提取材料的系统包括流体输送基底，其包括形成在流体输送基底内的入口通道和出口通道; 固定到流体输送基座上的垫圈，其中垫圈包括暴露入口通道的开口端和出口通道的开口端的至少一个开口; 支撑件，其包括面向所述垫圈的样品支撑表面和相对表面; 以及对准构件，其在固定位置联接到所述相对表面，并且使得所述支撑件定位在所述流体输送基座和所述对准构件之间，其中所述对准构件或所述流体输送基座中的一个或两个被朝向彼此偏置 力（例如，磁体或弹簧力），并且其中流体输送基座可与支撑件分离并且构造成沿着样品支撑表面的平面移动以与对准构件对准。
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公开(公告)号：US20170175105A1
公开(公告)日：2017-06-22
申请号：US15453719
申请日：2017-03-08
Applicant: General Electric Company
Inventor： John Richard Nelson , Wei Gao , Christopher Michael Puleo , Todd Frederick Miller , Christine Lynne Pitner , David Andrew Shoudy , Alex David Corwin
CPC classification number: C12N15/1013 , B01L3/502 , B01L2200/025 , B01L2200/0689 , B01L2300/06 , B01L2300/0654 , B01L2300/0861 , C12Q1/6806 , G01N1/02 , G01N1/10 , G01N1/40 , G01N2001/028 , G01N2001/4038
Abstract: A system for extracting material from a region of interest includes a fluid delivery base comprising an inlet channel and an outlet channel formed within the fluid delivery base, a gasket affixed to the fluid delivery base, wherein the gasket comprises at least one opening exposing an open end of the inlet channel and an open end of the outlet channel; a support comprising a sample-supporting surface facing the gasket and an opposing surface; and an alignment member coupled to the opposing surface in a fixed position and such that the support is positioned between the fluid delivery base and the alignment member, wherein one or both of the alignment member or the fluid delivery base are biased towards one another by a force (e.g., a magnet or spring force) and wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.
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公开(公告)号：US10598577B2
公开(公告)日：2020-03-24
申请号：US15639511
申请日：2017-06-30
Applicant: General Electric Company , University of Akron
Inventor： Arunkumar Natarajan , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Wei Gao , Kwok Pong Chan , Lakshmi Sireesha Kaanumalle , Abraham Joy , Nicholas Nun
Abstract: A method includes providing a biological sample, providing a sample collection device, wherein the sample collection device includes a sample binding surface including a photodegradable polymer configured to bind the biological sample, contacting the biological sample with the sample binding surface of the sample collection device, and irradiating the sample binding surface and the bound biological sample using light emitted from a light source to initiate degradation of the photodegradable polymer of the sample binding surface to cause release of the biological sample.
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