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公开(公告)号：US09279150B2
公开(公告)日：2016-03-08
申请号：US13840062
申请日：2013-03-15
Applicant: General Electric Company
Inventor： John Richard Nelson , Robert Scott Duthie , Gregory Andrew Grossman , Anuradha Sekher
CPC classification number: C12N9/22 , C07K1/00 , C07K14/00 , C12Q1/6806 , C12Q1/6844 , C12Q1/6858 , C12Y301/26 , C12Q2521/301 , C12Q2525/101 , C12Q2537/1376 , C12Q2527/101
Abstract: Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.
Abstract translation: 本文提供了能够切割含有肌苷的DNA序列的突变体内切核酸酶V酶。 还描述了使用这种突变体内切核酸酶V酶将切口引入到包括一种或多种肌苷的靶DNA中并使用DNA聚合酶产生靶DNA扩增子的核酸测定法和试剂。
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Patent Agency Ranking
公开(公告)号：US10518196B2
公开(公告)日：2019-12-31
申请号：US15209411
申请日：2016-07-13
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Christopher Michael Puleo , Craig Patrick Galligan , Gregory Andrew Grossman , Erik Leeming Kvam , Robert Scott Duthie , Kenneth Wayne Rigby , Paul Michael Smigelski, Jr. , Victoria Eugenia Cotero , Jason William Castle , John Donald Burczak , James Edward Rothman
IPC: B01D21/00 , B01D21/24 , G01N33/49 , B01L3/00 , G01N1/40 , C12M1/12 , C12M1/26 , B01D17/02 , B01D21/02 , C12M1/00 , C02F1/48 , C02F1/44 , C02F101/32
Abstract: A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.
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公开(公告)号：US20140093878A1
公开(公告)日：2014-04-03
申请号：US13840062
申请日：2013-03-15
Applicant: General Electric Company
Inventor： John Richard Nelson , Robert Scott Duthie , Gregory Andrew Grossman , Anuradha Sekher
CPC classification number: C12N9/22 , C07K1/00 , C07K14/00 , C12Q1/6806 , C12Q1/6844 , C12Q1/6858 , C12Y301/26 , C12Q2521/301 , C12Q2525/101 , C12Q2537/1376 , C12Q2527/101
Abstract: Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.
Abstract translation: 本文提供了能够切割含有肌苷的DNA序列的突变体内切核酸酶V酶。 还描述了使用这种突变体内切核酸酶V酶将切口引入到包括一种或多种肌苷的靶DNA中并使用DNA聚合酶产生靶DNA扩增子的核酸测定法和试剂。
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公开(公告)号：US10100292B2
公开(公告)日：2018-10-16
申请号：US15048624
申请日：2016-02-19
Applicant: General Electric Company
Inventor： John Richard Nelson , Robert Scott Duthie , Gregory Andrew Grossman , Anuradha Sekher
IPC: C12Q1/68 , C12N9/22 , C12Q1/6858 , C07K14/00 , C07K1/00 , C12Q1/6844 , C12Q1/6806
Abstract: Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.
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公开(公告)号：US20160160198A1
公开(公告)日：2016-06-09
申请号：US15048624
申请日：2016-02-19
Applicant: General Electric Company
Inventor： John Richard Nelson , Robert Scott Duthie , Gregory Andrew Grossman , Anuradha Sekher
CPC classification number: C12N9/22 , C07K1/00 , C07K14/00 , C12Q1/6806 , C12Q1/6844 , C12Q1/6858 , C12Y301/26 , C12Q2521/301 , C12Q2525/101 , C12Q2537/1376 , C12Q2527/101
Abstract: Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.
Abstract translation: 本文提供了能够切割含有肌苷的DNA序列的突变体内切核酸酶V酶。 还描述了使用这种突变体内切核酸酶V酶将切口引入到包括一种或多种肌苷的靶DNA中并使用DNA聚合酶产生靶DNA扩增子的核酸测定法和试剂。
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公开(公告)号：US20130130352A1
公开(公告)日：2013-05-23
申请号：US13729566
申请日：2012-12-28
Applicant: GENERAL ELECTRIC COMPANY
Inventor： John Richard Nelson , Robert Scott Duthie , Gregory Andrew Grossman
IPC: C12N9/12
CPC classification number: C12N9/1241 , C12Q1/6848 , C12Q2525/125 , C12Q2521/319
Abstract: Methods and kits for generating contamination-free reagents and reagent solutions for use in nucleic acid amplification are provided. Methods include processing of polymerase solutions, nucleotide solutions and primer solutions to render contaminating nucleic acid inert. The methods employ the proofreading activity of the polymerase and/or exonucleases to de-contaminate the reagents and reagent solutions. Methods and kits for contamination-free nucleic acid amplification are provided.
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