公开(公告)号：US20240200114A1

公开(公告)日：2024-06-20

申请号：US18285018

申请日：2022-04-01

申请人： GINKGO BIOWORKS, INC.

发明人： Guillaume Beaudoin ,  Alexandra Exner ,  Annapurna Kamineni ,  Matthew McMahon ,  Joshua Trueheart

IPC分类号： C12P19/56 ,  C12N9/02 ,  C12N9/10 ,  C12N9/14 ,  C12N9/90 ,  C12P19/60

CPC分类号： C12P19/56 ,  C12N9/0071 ,  C12N9/1051 ,  C12N9/14 ,  C12N9/90 ,  C12P19/60 ,  C12Y114/19 ,  C12Y204/01017 ,  C12Y303/02009 ,  C12Y504/99007

摘要： Described in this application are proteins and host cells involved in methods of producing mogrol precursors, mogrol, and/or mogrosides.

公开(公告)号：US20240150774A1

公开(公告)日：2024-05-09

申请号：US18344219

申请日：2023-06-29

申请人： Genomatica, Inc.

发明人： Derek L. GREENFIELD ,  Andreas W. Schirmer ,  Elizabeth J. Clarke ,  Eli S. Groban ,  Bernardo M. Da Costa ,  Zhihao Hu ,  Kevin Holden ,  Noah Helman

IPC分类号： C12N15/70 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12P7/04 ,  C12P7/6409 ,  C12P7/6436

CPC分类号： C12N15/70 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1288 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P7/04 ,  C12P7/6409 ,  C12P7/6436 ,  C12Y101/011 ,  C12Y103/01009 ,  C12Y103/0101 ,  C12Y203/01039 ,  C12Y203/01041 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y207/08007 ,  C12Y402/01059 ,  C12Y503/03014 ,  C12Y604/01002

摘要： The disclosure relates to recombinant host cells including strain modifications effective to improve titer, yield and/or productivity of fatty acid derivatives. The disclosure further relates to cell cultures including the recombinant host cells for the fermentative production of fatty acid derivatives and compositions thereof.

公开(公告)号：US11970723B2

公开(公告)日：2024-04-30

申请号：US18187984

申请日：2023-03-22

申请人： Jiangnan University

发明人： Tao Zhang ,  Mengying Hu ,  Bo Jiang ,  Mengli Li

IPC分类号： C12N9/90 ,  C12N15/75

CPC分类号： C12N9/90 ,  C12N15/75

摘要： The present disclosure discloses a strain producing D-allulose 3-epimerase and application thereof, and belongs to the technical field of bioengineering. The present disclosure provides a method for improving the expression of D-allulose 3-epimerase by screening promoters and optimizing RBS thereof. The recombinant Bacillus subtilis constructed using thevectors pP43NMK-hag and pP43NMK-hag-RBS4 provided by the present disclosure improves the enzyme activity of a target gene D-allulose 3-epimerase, and theenzyme activities in shake flasks upon transformation are 1.30 times and 1.69 times that of an original vector. The present disclosure further provides a non-antibiotic resistance vector and a non-antibiotic resistance recombinant B. subtilis strain. Using the non-antibiotic resistance strain B. subtilis 1A751-dal-/pP43NMK-hag-RBS4-dpe-dal provided by the present disclosure, the highest fermentation enzyme activity in a shake flask is 24.72 U/mL, and the enzyme activity in a fermenter is 714.8 U/mL.

公开(公告)号：US11965197B2

公开(公告)日：2024-04-23

申请号：US17197468

申请日：2021-03-10

申请人： RENSSELAER POLYTECHNIC INSTITUTE

发明人： Mattheos Koffas ,  John Andrew Jones

IPC分类号： C12N9/90 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/52 ,  C12P7/42 ,  C12P7/52 ,  C12P17/06 ,  C12P19/58

CPC分类号： C12P19/58 ,  C12N9/0006 ,  C12N9/0071 ,  C12N9/0093 ,  C12N9/1037 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12N15/52 ,  C12P7/42 ,  C12P7/52 ,  C12P17/06 ,  C12Y101/01219 ,  C12Y114/11009 ,  C12Y117/01003 ,  C12Y203/01074 ,  C12Y403/01023 ,  C12Y505/01006 ,  C12Y602/01012 ,  C12Y602/01014

摘要： Disclosed herein are novel microbial polycultures of two or more cell strains, capable of producing flavanones, flavonoids, and anthocyanidin-3-O-glucosides, and methods of use thereof. Also disclosed is a microbial cell capable of producing phenylpropanoic acids, and methods of use thereof.

公开(公告)号：US11959073B2

公开(公告)日：2024-04-16

申请号：US17244180

申请日：2021-04-29

申请人： SEATTLE CHILDREN'S HOSPITAL

发明人： Andrew M. Scharenberg ,  Michael T. Certo ,  Kamila Sabina Gwiazda

IPC分类号： C12N15/62 ,  A61K9/00 ,  A61K35/28 ,  A61K38/45 ,  A61K38/46 ,  A61K38/52 ,  C12N9/12 ,  C12N9/16 ,  C12N9/22 ,  C12N9/90 ,  C12N15/10 ,  A61K35/12

CPC分类号： C12N15/102 ,  A61K9/0019 ,  A61K35/28 ,  A61K38/45 ,  A61K38/465 ,  A61K38/52 ,  C12N9/1252 ,  C12N9/22 ,  C12N9/90 ,  C12N15/62 ,  A61K2035/124 ,  C07K2319/60 ,  C12N2800/80 ,  C12N2840/203 ,  A61K38/45 ,  A61K2300/00 ,  A61K38/465 ,  A61K2300/00 ,  A61K38/52 ,  A61K2300/00

摘要： The present disclosure relates to the co-expression of an endonuclease with an end-processing enzyme for the purpose of enhanced processing of the polynucleotide ends generated by endonuclease cleavage.

公开(公告)号：US20240099257A1

公开(公告)日：2024-03-28

申请号：US18163499

申请日：2023-02-02

申请人： Altria Client Services LLC

发明人： Jesse Frederick ,  Chengalrayan Kudithipudi ,  Dongmei Xu

IPC分类号： A01H6/82 ,  A01H5/10 ,  A01H5/12 ,  C07K14/415 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12N15/82

CPC分类号： A01H6/823 ,  A01H5/10 ,  A01H5/12 ,  C07K14/415 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  C12N15/8227 ,  C12N15/8237 ,  C12N15/8261 ,  C12Y205/01054 ,  C12Y402/01033 ,  C12Y501/03015

摘要： The present disclosure provides metabolic signatures and genetic markers for tracking enhanced nitrogen utilization efficiency phenotypes in tobacco plants and for introgressing enhanced nitrogen utilization efficiency phenotypes into tobacco plants. The disclosure also provides tobacco plants comprising enhanced nitrogen utilization efficiency and methods to the creation of tobacco plants comprising enhanced nitrogen utilization efficiency. The disclosure also provides recombinant polynucleotides and polypeptides for enhancing nitrogen utilization efficiency in modified tobacco plants and tobacco plants comprising the provided recombinant polynucleotides and polypeptides.

公开(公告)号：US11938153B2

公开(公告)日：2024-03-26

申请号：US16499760

申请日：2018-03-30

申请人： THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

发明人： Rachel Lynn ,  Crystal Mackall ,  Tom J. Wandless ,  Evan Weber

IPC分类号： A61K35/17 ,  A61K31/5025 ,  A61K31/506 ,  A61P35/00 ,  C07K14/705 ,  C07K14/725 ,  C07K16/30 ,  C12N5/0783 ,  C12N9/90

CPC分类号： A61K35/17 ,  A61K31/5025 ,  A61K31/506 ,  A61P35/00 ,  C07K14/7051 ,  C07K14/70521 ,  C07K14/70578 ,  C07K16/3084 ,  C12N5/0636 ,  C12N9/90 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/33 ,  C12N2501/727 ,  C12N2510/00

摘要： The present invention relates to T cell compositions and methods of using the same in the context of therapy and treatment. In particular, the invention provides chimeric antigen receptor (CAR) T cells that are modified to maintain functionality under conditions in which unmodified CAR T cells display exhaustion. Compositions and methods disclosed herein find use in inhibiting or reversing CAR T cell exhaustion (e.g., by modulating CAR surface expression) thereby enhancing CAR T cell function. Compositions and methods of the invention fmd use in both clinical and research settings, for example, within the fields of biology, immunology, medicine, and oncology.

公开(公告)号：US20240093258A1

公开(公告)日：2024-03-21

申请号：US18038067

申请日：2021-11-24

申请人： Manus Bio Inc.

发明人： Ajikumar PARAYIL KUMARAN ,  Christine Nicole S. SANTOS ,  Jason DONALD ,  Aaron LOVE ,  Yiying ZHENG ,  Adel GHADERI ,  Vineet SHASTRY ,  Lu CHEN ,  Christopher TOOMEY ,  Hannah LYNCH ,  Eric NIEMINEN

IPC分类号： C12P19/60 ,  C12N9/10 ,  C12N9/90

CPC分类号： C12P19/60 ,  C12N9/1051 ,  C12N9/90 ,  C12Y204/01017 ,  C12Y503/01009

摘要： In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)-dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.

公开(公告)号：US11919283B2

公开(公告)日：2024-03-05

申请号：US16300608

申请日：2017-05-11

申请人： ROCKWOOL INTERNATIONAL A/S

发明人： Thomas Hjelmgaard ,  Lars Hald ,  Thomas Tielemann

IPC分类号： B32B37/12 ,  B32B5/12 ,  B32B5/26 ,  B32B7/12 ,  B32B15/14 ,  B32B19/04 ,  B32B37/14 ,  B32B37/18 ,  B32B38/00 ,  C03C13/06 ,  C03C25/26 ,  C03C25/32 ,  C03C25/321 ,  C03C25/328 ,  C08J5/04 ,  C08L1/28 ,  C08L3/02 ,  C08L5/12 ,  C08L89/06 ,  C09J5/00 ,  C09J11/06 ,  C09J101/28 ,  C09J103/02 ,  C09J105/00 ,  C09J105/04 ,  C09J105/06 ,  C09J105/12 ,  C09J189/00 ,  C09J189/06 ,  D04H1/413 ,  D04H1/4209 ,  D04H1/4218 ,  D04H1/4266 ,  D04H1/587 ,  D04H1/593 ,  D04H1/64 ,  D04H1/724 ,  D04H1/74 ,  D04H3/002 ,  D04H3/004 ,  E04B1/74 ,  E04B1/80 ,  E04B1/88 ,  E04B1/94 ,  E04C2/284 ,  E04D3/35 ,  E04F13/08 ,  C12N9/02 ,  C12N9/06 ,  C12N9/08 ,  C12N9/10 ,  C12N9/90 ,  E04B1/76

CPC分类号： B32B37/12 ,  B32B5/12 ,  B32B5/26 ,  B32B7/12 ,  B32B15/14 ,  B32B19/04 ,  B32B19/041 ,  B32B37/1207 ,  B32B37/146 ,  B32B37/18 ,  B32B38/0004 ,  C03C13/06 ,  C03C25/26 ,  C03C25/32 ,  C03C25/321 ,  C03C25/328 ,  C08J5/043 ,  C08L1/286 ,  C08L3/02 ,  C08L5/12 ,  C08L89/06 ,  C09H11/00 ,  C09J5/00 ,  C09J11/06 ,  C09J101/28 ,  C09J101/286 ,  C09J103/02 ,  C09J105/00 ,  C09J105/04 ,  C09J105/06 ,  C09J105/12 ,  C09J189/005 ,  C09J189/06 ,  D04H1/413 ,  D04H1/4209 ,  D04H1/4218 ,  D04H1/4266 ,  D04H1/587 ,  D04H1/593 ,  D04H1/64 ,  D04H1/724 ,  D04H1/74 ,  D04H3/002 ,  D04H3/004 ,  E04B1/74 ,  E04B1/80 ,  E04B1/88 ,  E04B1/94 ,  E04C2/284 ,  E04D3/352 ,  E04F13/0866 ,  B32B2037/1215 ,  B32B2037/1253 ,  B32B2037/1269 ,  B32B38/164 ,  B32B2250/20 ,  B32B2250/40 ,  B32B2255/02 ,  B32B2255/26 ,  B32B2262/108 ,  B32B2305/20 ,  B32B2305/72 ,  B32B2307/304 ,  B32B2307/732 ,  B32B2309/02 ,  B32B2315/14 ,  B32B2317/00 ,  B32B2419/06 ,  B32B2607/00 ,  C03C2213/00 ,  C03C2218/11 ,  C08J2301/28 ,  C08J2303/02 ,  C08J2389/06 ,  C08J2405/00 ,  C08J2405/04 ,  C08J2405/06 ,  C08J2405/12 ,  C08J2491/00 ,  C08J2493/00 ,  C08L2201/52 ,  C08L2205/03 ,  C09J2400/146 ,  C09J2401/00 ,  C09J2403/00 ,  C09J2405/00 ,  C09J2489/00 ,  C12N9/0022 ,  C12N9/0051 ,  C12N9/0059 ,  C12N9/0065 ,  C12N9/0071 ,  C12N9/1044 ,  C12N9/90 ,  C12Y104/03013 ,  C12Y108/03002 ,  C12Y110/03001 ,  C12Y111/01007 ,  C12Y114/18001 ,  C12Y203/01013 ,  C12Y203/02013 ,  C12Y503/04001 ,  D10B2505/20 ,  E04B2001/742 ,  E04B2001/743 ,  E04B2001/745 ,  E04B2001/7683 ,  C09J189/06 ,  C08L5/12

摘要： The present invention relates to a method of providing thermal and/or acoustic insulation to a structure, comprising the steps of: providing a substrate which comprises fibres; applying the substrate to the structure; blending the substrate with a binder composition before, during or after application of the substrate to the structure; allowing curing of the binder composition after the substrate and the binder composition have been applied to the structure; wherein the binder composition comprises at least one hydrocolloid. The present invention also relates to an insulated structure obtainable by said method.

公开(公告)号：US20240060078A1

公开(公告)日：2024-02-22

申请号：US17906611

申请日：2021-03-17

申请人： The Coca-Cola Company

发明人： Alec J. HAYES ,  Christopher P. MERCOGLIANO ,  Tengfang HUANG ,  Fayaz KHAZI

IPC分类号： C12N15/82 ,  A23L27/30 ,  C12N9/02 ,  C12N9/10 ,  C12N9/14 ,  C12N9/90 ,  C12N15/52

CPC分类号： C12N15/8243 ,  A23L27/36 ,  C12N9/0071 ,  C12N9/1051 ,  C12N9/14 ,  C12N9/90 ,  C12N15/52 ,  C12Y504/99033

摘要： The present disclosure presents a solution to producing mogrosides and mogroside-based sweeteners having low or no calorie. By using recombinant gene and plant transformation techniques, non-native genes encoding mogroside pathway enzymes are introduced/implemented into the genome of a plant hereby forming a transgenic plant, wherein the plant by the native genome thereof prior to transformation may not produce mogrosides naturally. Such transgenic plant and a progeny thereof are enabled to produce non-native mogrol precursors, mogrol, mogrosides, and/or metabolites or derivatives thereof.