公开(公告)号：US20240200019A1

公开(公告)日：2024-06-20

申请号：US18288954

申请日：2022-04-28

申请人： Ginkgo Bioworks, Inc.

发明人： Annapurna Kamineni ,  Arthur J. Shaw ,  Vasiliki Tsakraklides

IPC分类号： C12N1/16 ,  C12N9/10 ,  C12N9/88 ,  C12N15/81 ,  C12P5/00 ,  C12P7/6454 ,  C12P7/6463 ,  C12R1/645

CPC分类号： C12N1/165 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/815 ,  C12P5/007 ,  C12P7/6454 ,  C12P7/6463 ,  C07K2319/00 ,  C12R2001/645 ,  C12Y203/01008 ,  C12Y401/02009

摘要： Aspects of the disclosure are directed to genetically modified yeast cells and methods for use. Certain aspects are directed to recombinant yeast cells comprising exogenous nucleic acid sequences encoding phosphotransacetylase and/or phosphoketolase proteins, including a phosphoketolase protein from Clostridium acetobutylicum. Also disclosed are methods for generating recombinant yeast cells and methods of use of such cells for production of one or more products, including lipids, oils, fatty acids, and triacylglycerides.

公开(公告)号：US11572546B2

公开(公告)日：2023-02-07

申请号：US16942509

申请日：2020-07-29

申请人： GINKGO BIOWORKS, INC.

发明人： Vasiliki Tsakraklides ,  Annapurna Kamineni

IPC分类号： C12N9/02 ,  C12P7/64 ,  C12N15/81

摘要： The current methods and compositions provide for nucleotide sequences of promoters from Yarrowia lipolytica which may be used to drive gene expression in a cell. In some aspects, the promoters are useful for modulating lipid production in oleaginous organisms such as yeast.

公开(公告)号：US11634737B2

公开(公告)日：2023-04-25

申请号：US15534818

申请日：2015-12-09

申请人： Ginkgo Bioworks, Inc.

发明人： Vasiliki Tsakraklides ,  Elena E. Brevnova ,  Jonathan Friedlander ,  Annapurna Kamineni ,  Arthur J. Shaw, IV

IPC分类号： C12N1/16 ,  C12P7/6463 ,  C12N9/10 ,  C12N15/80

摘要： Disclosed are transformed cells comprising one or more genetic modifications that affect the lipid content of the cell, e.g., by increasing the concentration of oleic acid in the cell relative to an unmodified cell of the same type. Also disclosed are methods for modifying the lipid content of a cell by increasing the activity of one or more proteins in the cell and/or by decreasing the activity of one or more proteins in the same cell.

公开(公告)号：US20240200114A1

公开(公告)日：2024-06-20

申请号：US18285018

申请日：2022-04-01

申请人： GINKGO BIOWORKS, INC.

发明人： Guillaume Beaudoin ,  Alexandra Exner ,  Annapurna Kamineni ,  Matthew McMahon ,  Joshua Trueheart

IPC分类号： C12P19/56 ,  C12N9/02 ,  C12N9/10 ,  C12N9/14 ,  C12N9/90 ,  C12P19/60

CPC分类号： C12P19/56 ,  C12N9/0071 ,  C12N9/1051 ,  C12N9/14 ,  C12N9/90 ,  C12P19/60 ,  C12Y114/19 ,  C12Y204/01017 ,  C12Y303/02009 ,  C12Y504/99007

摘要： Described in this application are proteins and host cells involved in methods of producing mogrol precursors, mogrol, and/or mogrosides.

公开(公告)号：US20240026395A1

公开(公告)日：2024-01-25

申请号：US18138472

申请日：2023-04-24

申请人： Ginkgo Bioworks, Inc.

发明人： Vasiliki Tsakraklides ,  Elena E. Brevnova ,  Jonathan Friedlander ,  Annapurna Kamineni ,  Arthur J. Shaw, IV

IPC分类号： C12P7/6463 ,  C12N9/10 ,  C12N15/80 ,  C12N1/16

CPC分类号： C12P7/6463 ,  C12Y203/01158 ,  C12Y203/01015 ,  C12Y203/01199 ,  C12N9/1029 ,  C12N15/80 ,  C12Y203/0102 ,  C12N1/16 ,  Y02E50/10

摘要： Disclosed are transformed cells comprising one or more genetic modifications that affect the lipid content of the cell, e.g., by increasing the concentration of oleic acid in the cell relative to an unmodified cell of the same type. Also disclosed are methods for modifying the lipid content of a cell by increasing the activity of one or more proteins in the cell and/or by decreasing the activity of one or more proteins in the same cell.