公开(公告)号：US09926344B2

公开(公告)日：2018-03-27

申请号：US14420831

申请日：2013-08-29

Applicant: GlaxoSmithKline Biologicals SA

Inventor： Fabio Bagnoli ,  Fabiana Falugi ,  Guido Grandi ,  Massimo Mariani ,  Mikkel Nissum ,  Michele Pallaoro ,  Silvana Savino

IPC: C07K14/31 ,  A61K39/085 ,  A61K39/00

CPC classification number: C07K14/31 ,  A61K39/085 ,  A61K2039/55505

Abstract: Elimination of disulphide bond formation of cysteine-containing S. aureus antigens enhances antigen stability. The invention provides variant forms of cysteine-containing S. aureus antigen with a point mutation that replaces, deletes or modifies the cysteine residue.

公开(公告)号：US20180064800A9

公开(公告)日：2018-03-08

申请号：US15127537

申请日：2015-03-24

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Fabio BAGNOLI ,  Luigi FIASCHI ,  Maria SCARSELLI

IPC: A61K39/085 ,  C07K14/31

CPC classification number: A61K39/085 ,  A61K35/74 ,  A61K2039/55505 ,  A61K2039/55511 ,  A61K2039/55577 ,  A61K2039/645 ,  A61K2039/70 ,  C07K14/31 ,  C07K2319/00 ,  C07K2319/40

Abstract: Mutant protein A of Staphylococcus aureus (SpA) with decreased affinity for the Fcγ portion of human IgG is provided.

公开(公告)号：US09890199B2

公开(公告)日：2018-02-13

申请号：US15103173

申请日：2013-12-09

Applicant: OLYMVAX BIOPHARMACEUTICALS INC. ,  THIRD MILITARY MEDICAL UNIVERSITY, PLA

Inventor： Quanming Zou ,  Hao Zeng ,  Shaowen Fan ,  Lu Lu ,  Qiang Feng ,  Jinyong Zhang ,  Haiming Jing ,  Yandong Dong ,  Yi Wu ,  Changzhi Cai

IPC: C07K14/31 ,  A61K39/085 ,  G01N33/569 ,  A61K39/40 ,  C07K16/12 ,  C12N15/70 ,  A61K39/00

CPC classification number: C07K14/31 ,  A61K39/085 ,  A61K39/40 ,  A61K2039/55505 ,  A61K2039/575 ,  C07K16/12 ,  C07K16/1271 ,  C07K2319/00 ,  C12N15/70 ,  G01N33/56938 ,  G01N2333/31 ,  G01N2469/20 ,  G01N2800/26

Abstract: Provided is staphylococcus protein A expressed by a mutational Staphylococcus aureus and its coding sequence, as well as a vector, host bacteria, composition or kit which contains the coding sequence of the mutational protein. Also provided is the use of the mutational protein and the composition thereof in the preparation of vaccines, therapeutic antibodies, diagnostic kits and the like, and for the prevention, treatment and detection of infections by Staphylococcus aureus. Also provided are methods for producing, fermenting and purifying the mutational protein.

公开(公告)号：US09873729B2

公开(公告)日：2018-01-23

申请号：US14776319

申请日：2014-03-17

Applicant: AFFIBODY AB

Inventor： Caroline Ekblad ,  Elin Gunneriusson ,  Malin Lindborg ,  Lars Abrahmsen ,  John Löblom ,  Torbjörn Gräslund ,  Johan Seijsing

IPC: C07K14/745 ,  C07K14/735 ,  C07K14/74 ,  C07K14/31 ,  C07K14/435 ,  A61K38/00

Abstract: The present disclosure relates to a class of engineered polypeptides having a binding affinity for the neonatal Fc receptor (in the following referred to as FcRn), and provides an FcRn binding polypeptide comprising the sequence EX2X3X4AX6X7 EIRWLPNL X16X17X18QRX21 AFIX25X26LX28X29 (SEQ ID NO: 1075). The present disclosure also relates to the use of such an FcRn binding polypeptide as an agent for modifying pharmacokinetic and pharmacodynamic properties and as a therapeutic agent.

公开(公告)号：US20170253673A1

公开(公告)日：2017-09-07

申请号：US15515936

申请日：2015-09-30

Applicant: BIOASTRA TECHNOLOGIES INC.

Inventor： Christopher KENT ,  Wilms BAILLE ,  Marc GAUTHIER ,  Yi ZHAO ,  Nicolas COTTENYE

IPC: C07K17/08 ,  C08G81/02 ,  C07K1/32 ,  C07K16/06 ,  B01D15/38 ,  C07K1/22 ,  C07K1/34 ,  C07K1/36 ,  B01D15/36 ,  C07K14/31 ,  C07K1/113

CPC classification number: C07K17/08 ,  B01D15/361 ,  B01D15/3809 ,  B01D15/3823 ,  B01D15/3876 ,  B01D15/388 ,  C07K1/113 ,  C07K1/22 ,  C07K1/32 ,  C07K1/34 ,  C07K1/36 ,  C07K14/195 ,  C07K14/31 ,  C07K14/315 ,  C07K16/00 ,  C07K16/065 ,  C07K2317/20 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2319/00 ,  C08G81/02 ,  C09D101/12 ,  C09D101/284 ,  C09D103/02

Abstract: There are provided efficient and cost-effective methods for purifying biomolecules in solution phase using stimuli-responsive protein-polymer conjugates. The protein-polymer conjugates comprise a target biomolecule-binding protein conjugated to a stimuli-responsive polymer and are reusable.

公开(公告)号：US09750795B2

公开(公告)日：2017-09-05

申请号：US14365071

申请日：2012-12-11

Applicant: The Trustees of the University of Pennsylvania

Inventor： David B. Weiner ,  Matthew P. Morrow

IPC: C12N15/57 ,  C12N15/31 ,  C12N15/62 ,  C12N9/52 ,  A61K39/085 ,  C12Q1/68 ,  C07K14/31 ,  A61K38/20

CPC classification number: A61K39/085 ,  A61K38/20 ,  A61K38/208 ,  A61K38/2086 ,  C07K14/31 ,  C12Q1/689

Abstract: Nucleic acid molecules which encode an MRSA PBP2a protein or a fragment thereof which comprises at least 245 amino acid are disclosed. Compositions comprising the nucleic acid molecules are disclosed. Novel proteins which comprise a MRSA PBP2a protein or a fragment thereof which comprises at least 245 amino acid are disclosed are disclosed. Methods of inducing an immune response against MRSA PBP2a are disclosed, as are methods of treating an individual who has been diagnosed with MRSA and methods of preventing MRSA infection in an individual.

公开(公告)号：US09745340B2

公开(公告)日：2017-08-29

申请号：US14436150

申请日：2013-10-25

Applicant: AFFIBODY AB

Inventor： Per Jonasson ,  Pär Eklund

IPC: C07K1/22 ,  C07K14/00 ,  C07K1/14 ,  C07K1/16 ,  C07K14/315 ,  C07K14/31

CPC classification number: C07K1/22 ,  C07K14/00 ,  C07K14/31 ,  C07K14/315 ,  C07K2319/70

Abstract: The disclosure provides a method of separation of at least one ABD-containing molecule present in a liquid from other constituents in the liquid, comprising a step of affinity separation, in which step is used, as affinity ligand, an ABD binding polypeptide comprising an ABD binding motif BM, which motif consists of an amino acid sequence selected from (SEQ ID NO: 166) EX2X3X4AX6X7EIX10X11LPNLX16X17X18QX20X21AFIX25X26LX28D and amino acid sequences with at least 89% identity thereto.

公开(公告)号：US20170175183A1

公开(公告)日：2017-06-22

申请号：US15439173

申请日：2017-02-22

Applicant: Jingyue Ju ,  Zengmin Li ,  Sergey Kalachikov ,  Carl Fuller

Inventor： Jingyue Ju ,  Zengmin Li ,  Sergey Kalachikov ,  Carl Fuller

IPC: C12Q1/68 ,  C12N9/12 ,  C12N9/96 ,  C07K14/31

CPC classification number: C12Q1/6874 ,  A61K47/545 ,  A61K47/62 ,  A61K47/6889 ,  A61K47/69 ,  C07K14/31 ,  C12N9/1252 ,  C12N9/96 ,  C12Y207/07007

Abstract: The present disclosure relates to compositions and methods based on a fast, efficient chemical reaction for conjugating a pore-forming protein, such as α-hemolysin, to a biomolecule, such as antibodies, receptors, and enzymes, such as DNA polymerase, and the use of such pore-forming protein conjugates in nanopore devices and methods.

公开(公告)号：US09670262B2

公开(公告)日：2017-06-06

申请号：US14471400

申请日：2014-08-28

Applicant: Lupin Limited

Inventor： Shardul Salunkhe ,  Bhaskarjyoti Prasad ,  Praveen Muneshwar ,  Anjali Apte Deshpande ,  Sriram Padmanabhan

IPC: C07H21/04 ,  C12N9/00 ,  C12P21/06 ,  A61K39/00 ,  A61K39/02 ,  C07K14/635 ,  C07K14/31 ,  C07K14/485 ,  C07K14/49 ,  C07K14/535 ,  C07K14/54 ,  C07K14/55 ,  C07K14/555 ,  C12N9/72 ,  C12N9/96

CPC classification number: C07K14/635 ,  C07K14/31 ,  C07K14/485 ,  C07K14/49 ,  C07K14/535 ,  C07K14/5403 ,  C07K14/5406 ,  C07K14/5409 ,  C07K14/5412 ,  C07K14/5431 ,  C07K14/55 ,  C07K14/555 ,  C07K2319/35 ,  C07K2319/50 ,  C12N9/6459 ,  C12N9/96 ,  C12P21/06 ,  C12Y304/21069

Abstract: The present invention relates to modified SAK gene having amino acid SEQ ID 2. The present invention further relates to process for cloning and expressing modified SAK gene fusion protein which imparts improved stability to the heterologous protein of interest. Further the invention relates to process of purification of recombinant heterologous proteins from bacterial inclusion bodies using modified SAK.

公开(公告)号：US09663559B2

公开(公告)日：2017-05-30

申请号：US15063471

申请日：2016-03-07

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Martin Hall ,  Sture Larsson ,  Andreas Muranyi ,  Gustav Rodrigo ,  Jinyu Zou ,  Per-Mikael Aberg

IPC: C07K14/31 ,  B01D15/38 ,  B01J20/286 ,  C07K1/22 ,  B01J20/32 ,  C07K16/00 ,  C07K17/00

CPC classification number: B01J20/24 ,  B01D15/3809 ,  B01J20/28019 ,  B01J20/285 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/52 ,  B01J2220/54 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K17/00 ,  C07K17/10 ,  C07K2317/55

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.