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公开(公告)号：US10308690B2
公开(公告)日：2019-06-04
申请号：US14961164
申请日：2015-12-07
发明人： Gustav Rodrigo , Mats Ander , Göran Baurén , Tomas Björkman
IPC分类号： C07K14/31 , B01J20/32 , B01D15/38 , C07K1/22 , B01J20/286 , C07K14/745 , C07K16/00
摘要： The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, comprising at least the mutation wherein the glutamine residue at position 9 has been mutated to a tryptophan, leucine, glutamic acid, valine or lysine. The invention also discloses multimers of the polypeptide, as well as separation matrices comprising the multimers or polypeptides.
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公开(公告)号：US20190144511A1
公开(公告)日：2019-05-16
申请号：US16252015
申请日：2019-01-18
发明人： Gustav Rodrigo , Mats Ander , Tomas Bjorkman
IPC分类号： C07K14/195 , C07K16/00 , C07K1/22
摘要： A kappa light chain-binding polypeptide comprising or consisting essentially of one or more mutated binding domains of Peptostreptococcus Protein L.
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公开(公告)号：US10112980B2
公开(公告)日：2018-10-30
申请号：US15598685
申请日：2017-05-18
发明人： Mats Ander , Goran Bauren , Tomas Bjorkman , Gustav Rodrigo
IPC分类号： C07K14/31 , C07K14/745 , C07K16/00 , C07K1/22 , B01D15/38 , B01J20/32 , B01J20/286
摘要： The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamic acid residue at position 15 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.
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公开(公告)号：US20170320919A1
公开(公告)日：2017-11-09
申请号：US15526895
申请日：2015-12-11
发明人： Gustav Rodrigo , Mats Ander , Tomas Bjorkman
IPC分类号： C07K14/195
CPC分类号： C07K14/195 , C07K1/22 , C07K16/00 , C07K2317/55 , C07K2317/569 , C07K2319/02
摘要： The invention discloses kappa light chain-binding polypeptide comprising a mutated binding domain of Peptostreptococcus protein L, wherein at least one asparagine residue of a parental domain defined by, or having at least 95% or 98% sequence homology with, SEQ ID NO: 2-6 or 2 has been mutated to another amino acid residue which is not asparagine, proline or cysteine.
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公开(公告)号：US10730908B2
公开(公告)日：2020-08-04
申请号：US15798784
申请日：2017-10-31
发明人： Annika Forss , Mats Ander , Tomas Bjorkman , Hans Blom , Jesper Hansson , Gustav Rodrigo
IPC分类号： C07K1/16 , B01D15/08 , C07K1/22 , C07K16/00 , B01J20/286 , B01J20/285 , B01D15/18 , B01D15/34 , C07K14/31
摘要： The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.
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公开(公告)号：US10208091B2
公开(公告)日：2019-02-19
申请号：US15526849
申请日：2015-12-11
发明人： Gustav Rodrigo , Mats Ander , Tomas Bjorkman
IPC分类号： C07K14/195 , C07K1/22 , C07K16/00
摘要： A kappa light chain-binding polypeptide comprising or consisting essentially of one or more binding domains of Peptostreptococcus Protein L, each of said domains being selected from the group consisting of domain 2, domain 3 and domain 4.
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公开(公告)号：US20180327512A1
公开(公告)日：2018-11-15
申请号：US15525688
申请日：2015-11-11
发明人： Gustav Rodrigo , Tomas Bjorkman , Mats Ander
IPC分类号： C07K16/46 , B01J20/289 , B01J20/32 , B01D15/38
CPC分类号： C07K16/46 , B01D15/3809 , B01J20/289 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/31 , C07K16/00 , C07K16/065
摘要： An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.
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公开(公告)号：US20170334954A1
公开(公告)日：2017-11-23
申请号：US15526941
申请日：2015-11-16
发明人： Gustav Rodrigo , Tomas Bjorkman , Mats Ander
CPC分类号： C07K16/46 , B01D15/3809 , B01J20/289 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/31 , C07K16/00 , C07K16/065
摘要： An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:22, SEQ ID NO 51 or SEQ ID NO 52 wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO:4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
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公开(公告)号：US20190023735A1
公开(公告)日：2019-01-24
申请号：US16115756
申请日：2018-08-29
IPC分类号： C07K1/16 , B01J20/32 , C07K1/36 , B01J20/28 , B01J20/26 , B01D15/38 , G01N33/544 , B01J20/289 , B01J41/20 , B01D15/36 , B01D15/32
摘要： The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R1-L1-N(R3)-L2-R, immobilized on a support, wherein R1 is a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L1 is either a methylene group or a covalent bond; R2 is a five-or six-membered, substituted or non-substituted ring structure; L2 is either a methylene group or a covalent bond; R3 is a methyl group; and wherein if R1 is a hydroxyethyl group and L1 is a covalent bond, R2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.
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公开(公告)号：US10124328B2
公开(公告)日：2018-11-13
申请号：US15407240
申请日：2017-01-16
IPC分类号： B01J39/26 , B01D15/18 , B01D15/36 , B01J20/286 , B01J20/32 , B01D15/38 , B01J20/26 , B01J20/28 , B01J20/289 , B01J20/30 , C07H1/08 , C07K1/22 , B01D15/34 , B01J20/24 , G01N30/88
摘要： The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.
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