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公开(公告)号：US10343142B2
公开(公告)日：2019-07-09
申请号：US16189894
申请日：2018-11-13
发明人： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
IPC分类号： B01J20/24 , B01D15/38 , B01J20/286 , C07K1/22 , B01J20/32 , C07K14/31 , C07K16/00 , C07K17/00 , B01J20/28 , B01J20/285 , B01J20/289 , C07K17/10
摘要： The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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2.发明申请公开(公告)号：US20190060869A1
公开(公告)日：2019-02-28
申请号：US16189894
申请日：2018-11-13
发明人： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
IPC分类号： B01J20/24 , B01D15/38 , C07K16/00 , B01J20/32 , C07K17/10 , B01J20/28 , B01J20/285 , B01J20/289 , C07K14/31 , C07K1/22 , B01J20/286 , C07K17/00
CPC分类号： B01J20/24 , B01D15/3809 , B01J20/28019 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , B01J2220/54 , C07K1/22 , C07K14/31 , C07K16/00 , C07K17/00 , C07K17/10 , C07K2317/55
摘要： The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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公开(公告)号：US10213765B2
公开(公告)日：2019-02-26
申请号：US15603285
申请日：2017-05-23
发明人： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
IPC分类号： C07K1/22 , B01D15/38 , B01J20/24 , B01J20/286 , B01J20/32 , C07K14/31 , C07K16/00 , C07K17/00 , B01J20/28 , B01J20/285 , B01J20/289 , C07K17/10
摘要： The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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4.发明申请公开(公告)号：US20170259244A1
公开(公告)日：2017-09-14
申请号：US15603285
申请日：2017-05-23
发明人： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
IPC分类号： B01J20/24 , C07K14/31 , C07K17/10 , B01D15/38 , B01J20/28 , B01J20/285 , B01J20/289 , B01J20/32 , C07K1/22 , C07K16/00
CPC分类号： B01J20/24 , B01D15/3809 , B01J20/28019 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , B01J2220/54 , C07K1/22 , C07K14/31 , C07K16/00 , C07K17/00 , C07K17/10 , C07K2317/55
摘要： The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIF) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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公开(公告)号：US20190263856A1
公开(公告)日：2019-08-29
申请号：US16348534
申请日：2017-11-14
发明人： Johan Ohman , Mats Ander , Therese Graner , Anneli Jorsback , Jinyu Zou
IPC分类号： C07K1/22 , B01J20/24 , B01J20/285 , B01J20/286 , B01J20/32 , B01D15/38
摘要： The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells.
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公开(公告)号：US09663559B2
公开(公告)日：2017-05-30
申请号：US15063471
申请日：2016-03-07
发明人： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
CPC分类号： B01J20/24 , B01D15/3809 , B01J20/28019 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , B01J2220/54 , C07K1/22 , C07K14/31 , C07K16/00 , C07K17/00 , C07K17/10 , C07K2317/55
摘要： The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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