公开(公告)号：US20240060064A1

公开(公告)日：2024-02-22

申请号：US18467259

申请日：2023-09-14

申请人： Codexis, Inc.

发明人： Scott J. Novick ,  Xiang Yi ,  Nikki Dellas ,  Oscar Alvizo ,  Jovana Nazor ,  Da Duan ,  Vesna Mitchell ,  Jonathan Vroom ,  Santhosh Sivaramakrishnan ,  Nandhitha Subramanian ,  Jeffrey C. Moore ,  Mark Huffman ,  Agustina Rodriguez-Granillo ,  Deeptak Verma ,  Grant S. Murphy ,  Nicholas Marshall ,  Jay Russell ,  Keith A. Canada

IPC分类号： C12N9/90 ,  C12N15/52 ,  C12N15/74 ,  C12N15/81

CPC分类号： C12N9/90 ,  C12N15/52 ,  C12N15/74 ,  C12N15/815 ,  C12Y504/02007

摘要： The present invention provides engineered phosphopentomutase (PPM) enzymes, polypeptides having PPM activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PPM enzymes are also provided. The present invention further provides compositions comprising the PPM enzymes and methods of using the engineered PPM enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

公开(公告)号：US20240052333A1

公开(公告)日：2024-02-15

申请号：US18271132

申请日：2021-12-28

申请人： YAMAGUCHI UNIVERSITY

发明人： Osao Adachi ,  Toshiharu Yakushi ,  Naoya Kataoka ,  Kazunobu Matsushita

IPC分类号： C12N9/90 ,  C12N11/02 ,  C12P19/02

CPC分类号： C12N9/90 ,  C12N11/02 ,  C12P19/02 ,  C12Y503/01007

摘要： The present invention has an object to provide a D-mannose isomerase of membrane-bound form having the optimal reaction pH in acidic region.
The membrane-bound D-mannose isomerase derived from acetic acid bacteria is produced. The acetic acid bacteria are preferably belong to the genera of Acetobacter, Gluconobacter, or Gluconacetobacter. Furthermore, the membrane-bound D-mannose isomerase from the acetic acid bacteria is used to produce D-fructose from D-mannose.

公开(公告)号：US11884949B2

公开(公告)日：2024-01-30

申请号：US16984900

申请日：2020-08-04

申请人： Antheia, Inc.

发明人： Christina D. Smolke ,  Isis Trenchard ,  Kristy M. Hawkins ,  Catherine Thodey

IPC分类号： C12P17/12 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52

CPC分类号： C12P17/12 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y402/99

摘要： A method of producing promorphinan, morphinan, nal-opioid, and nor-opioid alkaloid products through the increased conversion of a promorphinan alkaloid to a morphinan alkaloid. The method comprises contacting the promorphinan alkaloid with at least one enzyme. Contacting the promorphinan alkaloid with the at least one enzyme converts the promorphinan alkaloid to a morphinan alkaloid.

公开(公告)号：US20230399669A1

公开(公告)日：2023-12-14

申请号：US18207453

申请日：2023-06-08

申请人： Chr. Hansen HMO GmbH

发明人： Stefan Jennewein ,  Dirk Wartenberg ,  Katja Parschat

IPC分类号： C12P19/12 ,  C12N1/20 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/90 ,  C12P19/04 ,  C12P19/18

CPC分类号： C12P19/12 ,  C12N1/20 ,  C12N9/1051 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/90 ,  C12P19/04 ,  C12P19/18 ,  C12N2500/34 ,  C12Y204/01038 ,  C12Y207/01004 ,  C12Y207/01011 ,  C12Y207/01056 ,  C12Y207/07009 ,  C12Y301/03011 ,  C12Y501/03002 ,  C12Y504/02002

摘要： Disclosed are genetically engineered microbial cells for the production of oligosaccharides comprising a galactose-β1,4-glucose moiety at their reducing end, wherein said microbial cells are able to produce said oligosaccharides in the absence of exogenously added lactose, and a method of producing said oligosaccharides using said microbial cells.

公开(公告)号：US20230332188A1

公开(公告)日：2023-10-19

申请号：US18043978

申请日：2021-09-03

申请人： Novozymes A/S ,  Microbiogen Pty. Ltd.

发明人： Roberto Nobuyuki Maeda ,  Philip John Livingstone Bell

IPC分类号： C12P7/10 ,  C12N1/18 ,  C12N15/81 ,  C12N9/12 ,  C12N9/10 ,  C12N9/90

CPC分类号： C12P7/10 ,  C12N1/185 ,  C12N15/81 ,  C12N9/1205 ,  C12N9/1022 ,  C12N9/90 ,  C12Y202/01001 ,  C12Y202/01002 ,  C12Y501/03001 ,  C12Y503/01006 ,  C12Y503/02005 ,  C12Y207/01017 ,  C12Y503/01005 ,  C12R2001/865

摘要： The present invention relates to processes for producing ethanol comprising saccharifying cellulosic or starch-containing material and fermenting the saccharified material with a fermenting microorganism to produce ethanol. The fermenting organism is Saccharomyces cerevisiae strain MBG5151 (deposited under Accession No. Y-67971 at the Agricultural Research Service Culture Collection (NRRL), Illinois 61604 U.S.A.), Saccharomyces cerevisiae strain MBG5248 (deposited under Accession No. Y-68015 at the Agricultural Research Service Culture Collection (NRRL), Illinois 61604 U.S.A.) or a fermenting organism that has properties that the same or about the same as that of Saccharomyces cerevisiae MBG5151 or MBG5248.

公开(公告)号：US20230313254A1

公开(公告)日：2023-10-05

申请号：US18054195

申请日：2022-11-10

申请人： Conagen Inc.

发明人： Guohong Mao ,  Michael Batten ,  Oliver Yu ,  Timothy Joseph Hanly ,  Phillip James Hunt ,  Hongfang Liu ,  Yang Luo

IPC分类号： C12P19/24 ,  C12N9/90 ,  C12N15/70 ,  C12N15/81

CPC分类号： C12P19/24 ,  C12N9/90 ,  C12N15/70 ,  C12N15/81 ,  C12Y501/03 ,  C12R2001/19

摘要： Provided herein are methods for identifying and isolating polynucleotides coding for polypeptides having D-allulose 3-epimerase activity from a wide variety of microorganisms. Also provided are nucleic acid constructs, vectors and recombinant host cells comprising the polynucleotides coding for D-allulose 3-epimerase activity as well as methods for producing allulose from fructose using said recombinant host cells having D-allulose 3-epimerase activity or the D-allulose 3-epimerase enzyme of said recombinant host cells having D-allulose 3-epimerase activity.

公开(公告)号：US20230313210A1

公开(公告)日：2023-10-05

申请号：US18024647

申请日：2021-09-03

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

发明人： Eric H. Lyons ,  Scott Zentack ,  David A. Baltrus

IPC分类号： C12N15/78 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  A61K8/99 ,  A61Q11/00 ,  A23K10/16 ,  A23K50/40

CPC分类号： C12N15/78 ,  C12N9/1007 ,  C12N9/88 ,  C12Y402/99021 ,  C12N9/90 ,  C12Y504/04002 ,  C12N9/1029 ,  C12Y203/01084 ,  A61K8/99 ,  A61Q11/00 ,  A23K10/16 ,  A23K50/40

摘要： Described herein are engineered bacteria that can produce a volatile compound and formulations thereof. Also described herein are methods of improving the breath odor of a mammal by administering the engineered bacteria or formulation thereof to the oral cavity of the mammal.

公开(公告)号：US20230310555A1

公开(公告)日：2023-10-05

申请号：US18003835

申请日：2021-06-30

申请人： CIBUS BIOTECHNOLOGIES, INC.

发明人： F.C. Thomas ALLNUTT

IPC分类号： A61K38/46 ,  C12N15/85 ,  C12N15/11 ,  C12N9/22 ,  C12N15/90 ,  C12N9/12 ,  C12N9/90 ,  A61K31/7088 ,  A61K48/00 ,  A61P31/20

CPC分类号： A61K38/465 ,  C12N15/85 ,  C12N15/11 ,  C12N15/111 ,  C12N9/22 ,  C12N15/907 ,  C12N9/1252 ,  C12N9/90 ,  C12Y599/01003 ,  A61K31/7088 ,  A61K48/0066 ,  A61P31/20 ,  C12N2800/107 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2800/22

摘要： The present disclosure concerns methods and compositions for inhibiting replication of viruses in mammalian cells. In some cases the virus can be African Swine Fever virus, or related viruses. The methods described herein can make use of programmable nucleases.

公开(公告)号：US11773421B2

公开(公告)日：2023-10-03

申请号：US17263401

申请日：2019-07-26

申请人： Global Bioenergies

发明人： Alfredo Alarcon Yanez ,  Samia Boudah ,  Romain Chayot

IPC分类号： C12P19/02 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90

CPC分类号： C12P19/02 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90 ,  C12Y202/01002 ,  C12Y301/03001 ,  C12Y301/03002 ,  C12Y301/03021 ,  C12Y301/03023 ,  C12Y301/03038 ,  C12Y301/03074 ,  C12Y401/02 ,  C12Y504/02002 ,  C12Y504/02008

摘要： Described is a method for the production of fructose-6-phosphate (F6P) from dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P) comprising the steps of:

(a) enzymatically converting dihydroxyacetone phosphate (DHAP) into dihydroxyacetone (DHA); and
(b) enzymatically converting the thus produced dihydroxyacetone (DHA) and glyceraldehyde-3-phosphate (G3P) into fructose-6-phosphate (F6P); or

comprising the steps of:

(a′) enzymatically converting glyceraldehyde-3-phosphate (G3P) into glyceraldehyde; and
(b′) enzymatically converting the thus produced glyceraldehyde together with dihydroxyacetone phosphate (DHAP) into fructose-1-phosphate (F1P); and
(c′) enzymatically converting the thus produced fructose-1-phosphate (F1P) into fructose-6-phosphate (F6P).

公开(公告)号：US20230279456A1

公开(公告)日：2023-09-07

申请号：US18061585

申请日：2022-12-05

申请人： Jiangnan University

发明人： Tao ZHANG ,  Bo JIANG ,  Miaomiao HU ,  Mengli LI

IPC分类号： C12P19/26 ,  C12N15/70 ,  C12N9/10 ,  C12N9/12 ,  C12N9/38 ,  C12N9/90

CPC分类号： C12P19/26 ,  C12N15/70 ,  C12N9/1051 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/2471 ,  C12N9/90 ,  C12Y204/01038 ,  C12Y204/01222 ,  C12Y207/01006 ,  C12Y501/03002 ,  C12Y504/02002 ,  C12Y207/07012

摘要： The disclosure discloses genetically engineered bacteria producing lacto-N-neotetraose and a production method thereof, and belongs to the fields of metabolic engineering and food biotechnology. To solve the problem of low yield of lacto-N-neotetraose produced by a microbial method in the prior art, through exogenous expression of lgtA and lgtB, reasonable combination and regulation of overexpression of lacY, pgm, galE, galT and galK in a lacto-N-neotetraose synthesis pathway, knockout of lacZ expression in an Escherichia coli host, and optimization of a carbon source in the culture process, the disclosure achieves the objectives of regulating the carbon flux of a metabolic pathway and improving the yield of lacto-N-neotetraose. In a shake flask experiment, the yield of lacto-N-neotetraose produced by E. coli increased from 304 mg/L to 1031 mg/L, laying a foundation for industrial production of the lacto-N-neotetraose.