公开(公告)号：US06537754B1

公开(公告)日：2003-03-25

申请号：US09535789

申请日：2000-03-28

申请人： Andrei Gudkov ,  Igor B. Roninson

发明人： Andrei Gudkov ,  Igor B. Roninson

IPC分类号： C12Q168

CPC分类号： C12N15/113 ,  A61K38/00 ,  C07K14/47 ,  C12N9/90 ,  C12N15/1034 ,  C12N15/1137 ,  C12N15/1138 ,  C12N15/67 ,  C12N2310/18 ,  C12N2310/321 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Y599/01003 ,  G01N2800/44 ,  Y10T436/143333

摘要： The invention provides diagnostic assays for assessing the sensitivity or resistance to DNA damaging agents and microtubule-directed chemotherapeutic drugs of malignant cells in a tumor or tissue. The assay provided involves determining gene expression levels of kinesin genes in the malignant cells, wherein under-expression of kinesin is found in cells resistant to DNA damaging agents and sensitive to microtubule-directed chemotherapeutic drugs, and over-expression of kinesin is found in cells sensitive to DNA damaging agents and resistant to microtubule-directed chemotherapeutic drugs.

摘要翻译： 本发明提供用于评估肿瘤或组织中恶性细胞的DNA损伤剂和微管导向化学治疗药物的敏感性或抗性的诊断测定。 提供的测定涉及确定恶性细胞中驱动蛋白基因的基因表达水平，其中在对DNA损伤剂有抗性且对微管导向的化学治疗药物敏感的细胞中发现驱动蛋白的低表达，并且在细胞中发现驱动蛋白的过表达 对DNA损伤剂敏感，对微管导向的化学治疗药具有抗性。

公开(公告)号：US5811234A

公开(公告)日：1998-09-22

申请号：US39385

申请日：1993-09-07

申请人： Igor B. Roninson ,  Tatyana Holzmayer ,  Choi Kyunghee ,  Andrei Gudkov

发明人： Igor B. Roninson ,  Tatyana Holzmayer ,  Choi Kyunghee ,  Andrei Gudkov

IPC分类号： A61K48/00 ,  A61P31/12 ,  C07K14/00 ,  C12N1/21 ,  C12N5/10 ,  C12N9/90 ,  C12N15/00 ,  C12N15/09 ,  C12N15/10 ,  C12N15/113 ,  C12N15/63 ,  C12N15/67 ,  C12N15/867 ,  C12P21/02 ,  C12Q1/02 ,  F02D41/20 ,  F16K31/06 ,  G02B6/44 ,  H01F7/18 ,  C12Q1/68

CPC分类号： H01F7/1877 ,  C12N15/1072 ,  C12N15/1079 ,  C12N15/1082 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/1138 ,  C12N15/67 ,  C12N9/90 ,  C12Y599/01003 ,  C12N2310/18 ,  C12N2310/321

摘要： Methods for isolating and identifying genetic elements that are capable of inhibiting gene function are disclosed, as well as genetic elements isolated or identified according to the method of the invention and host cells modified by genetic modification using genetic suppressor elements according to the invention.

摘要翻译： PCT No.PCT / US91 / 07492 Sec。 371日期：1993年7月9日 102（e）日期1993年7月9日PCT 1991年10月11日PCT PCT。 出版物WO92 / 07071 日期：1992年04月30日公开了分离和鉴定能够抑制基因功能的遗传元件的方法，以及根据本发明方法分离或鉴定的遗传元件，以及使用遗传抑制因子通过遗传修饰修饰的宿主细胞 本发明。

公开(公告)号：US5753432A

公开(公告)日：1998-05-19

申请号：US204740

申请日：1994-03-02

申请人： Andrei Gudkov ,  Alexander Kazarov ,  Ilya Mazo ,  Igor B. Roninson

发明人： Andrei Gudkov ,  Alexander Kazarov ,  Ilya Mazo ,  Igor B. Roninson

IPC分类号： C12N15/09 ,  A61K31/70 ,  A61K38/00 ,  A61K48/00 ,  A61P35/00 ,  C07H21/04 ,  C07K14/47 ,  C12N5/10 ,  C12N9/90 ,  C12N15/10 ,  C12N15/113 ,  C12N15/67 ,  C12P21/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/53 ,  G01N33/574 ,  C12Q1/68 ,  C12N15/12 ,  C12N15/64

CPC分类号： C12N9/90 ,  C07K14/4703 ,  C12N15/1034 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/1138 ,  C12N15/67 ,  C12Y599/01003 ,  G01N33/574 ,  A61K38/00 ,  C12N2310/18 ,  C12N2310/321 ,  G01N2800/44

摘要： The invention provides genetic suppressor elements that confer the transformed phenotype of malignant mammalian cells upon untransformed cells, methods for identifying and obtaining such elements, methods for isolating and identifying genes corresponding to such elements, and methods of using such elements. The invention also provides genes corresponding to the GSEs of the invention.

公开(公告)号：US20230310555A1

公开(公告)日：2023-10-05

申请号：US18003835

申请日：2021-06-30

申请人： CIBUS BIOTECHNOLOGIES, INC.

发明人： F.C. Thomas ALLNUTT

IPC分类号： A61K38/46 ,  C12N15/85 ,  C12N15/11 ,  C12N9/22 ,  C12N15/90 ,  C12N9/12 ,  C12N9/90 ,  A61K31/7088 ,  A61K48/00 ,  A61P31/20

CPC分类号： A61K38/465 ,  C12N15/85 ,  C12N15/11 ,  C12N15/111 ,  C12N9/22 ,  C12N15/907 ,  C12N9/1252 ,  C12N9/90 ,  C12Y599/01003 ,  A61K31/7088 ,  A61K48/0066 ,  A61P31/20 ,  C12N2800/107 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2800/22

摘要： The present disclosure concerns methods and compositions for inhibiting replication of viruses in mammalian cells. In some cases the virus can be African Swine Fever virus, or related viruses. The methods described herein can make use of programmable nucleases.

公开(公告)号：US20230227799A1

公开(公告)日：2023-07-20

申请号：US18047589

申请日：2022-10-18

申请人： Oxford Nanopore Technologies PLC

发明人： Andrew John Heron ,  James Anthony Clarke ,  Ruth Moysey ,  Elizabeth Jayne Wallace ,  Mark John Bruce ,  Lakmal Jayasinghe ,  Domenico Caprotti ,  Szabolcs Soeroes ,  Luke McNeill ,  David Antoni Alves ,  Rebecca Victoria Bowen ,  John Milton

IPC分类号： C12N9/14 ,  C12N9/90 ,  C12Q1/6827

CPC分类号： C12N9/14 ,  C12Y306/04012 ,  C12N9/90 ,  C12Y599/01003 ,  C12Y599/01002 ,  C12Q1/6827 ,  C07K2319/80

摘要： The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

公开(公告)号：US20180305755A1

公开(公告)日：2018-10-25

申请号：US16009735

申请日：2018-06-15

申请人： Fenfei LENG ,  Xiaoduo ZHI ,  Samantha DAGES ,  Kelley DAGES

发明人： Fenfei LENG ,  Xiaoduo ZHI ,  Samantha DAGES ,  Kelley DAGES

IPC分类号： C12Q1/6876 ,  G01N33/50 ,  C12N15/63 ,  C12N15/10 ,  C12Q1/533 ,  C12Q1/02 ,  C12N9/90 ,  C12Q1/66 ,  C12N15/70

CPC分类号： C12Q1/6876 ,  C12N9/90 ,  C12N15/1086 ,  C12N15/63 ,  C12N15/70 ,  C12Q1/025 ,  C12Q1/533 ,  C12Q1/66 ,  C12Q2600/158 ,  C12Y599/01003 ,  G01N33/5023

摘要： The disclosure describes the effects of transcription mediated from a promoter on the transcription mediated by divergently coupled supercoiling-sensitive promoter. Transcription initiated from a promoter inhibits transcription mediated by a specific supercoiling-sensitive promoter that is divergently coupled to the promoter. A gyrase inhibitor relieves this inhibition and substantially increases the transcription mediated by the specific supercoiling-sensitive promoter that is divergently coupled to another promoter. Accordingly, the invention pertains to a method for identifying a compound as a gyrase inhibitor or not a gyrase inhibitor based on differential expression of genes under the control of divergently coupled promoters in the presence of the compound. Another embodiment of the invention provides an assay for identifying one or more compounds from a library of compounds as a gyrase inhibitor. Polynucleotides and cells containing such polynucleotides that are suitable for carrying out the methods described herein are also provided.

公开(公告)号：US20180023091A1

公开(公告)日：2018-01-25

申请号：US15547084

申请日：2016-01-29

申请人： MEIOGENIX ,  INSTITUT CURIE ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6)

发明人： GIACOMO BASTIANELLI ,  ALEXANDRE SERERO ,  ALAIN NICOLAS

IPC分类号： C12N15/82 ,  C12N9/90 ,  C12N15/90 ,  C12N15/81 ,  C12N15/65 ,  C12N9/22 ,  C12N15/11

CPC分类号： C12N15/8213 ,  C07K2319/09 ,  C12N9/22 ,  C12N9/90 ,  C12N15/102 ,  C12N15/11 ,  C12N15/63 ,  C12N15/65 ,  C12N15/81 ,  C12N15/902 ,  C12N15/905 ,  C12N2310/20 ,  C12N2800/22 ,  C12Y599/01003

摘要： The present invention relates to a fusion protein comprising a Cas9 domain and a Spo11 domain, as well as the use of this protein to induce targeted meiotic recombinations in a eukaryotic cell.

公开(公告)号：US08765443B2

公开(公告)日：2014-07-01

申请号：US13556687

申请日：2012-07-24

申请人： Nei-Li Chan ,  Tsai-Kun Li ,  Chyuan-Chuan Wu ,  Ying-Ren Wang

发明人： Nei-Li Chan ,  Tsai-Kun Li ,  Chyuan-Chuan Wu ,  Ying-Ren Wang

IPC分类号： C07H21/04 ,  C12N9/90 ,  C07H15/252 ,  G01N23/20

CPC分类号： C12Y599/01003 ,  C07K2299/00 ,  C12N9/90 ,  Y10T436/143333

摘要： Disclosed herein are a crystal of a human TOPII (hTOPII)-DNA binary complex, the method for preparing the same and the use thereof. The hTOPII-DNA binary complex includes an hTOPII portion that contains an hTOPII core domain (hTOPIIcore), and a synthetic double-stranded DNA in complex with the hTOPII portion. The synthetic double-stranded DNA has a first DNA strand comprising nucleotide positions 3 to 20 of the sequence of 5′-NNNCCGAGCNNNNGCTCGGNNN-3′ (SEQ ID NO: 1), wherein N is any one of adenine, thymine, cytosine, or guanine, and a second DNA strand complementary to the first DNA strand.

摘要翻译： 本文公开了人TOPII（hTOPII）-DNA二元复合物的结晶，其制备方法及其用途。 hTOPII-DNA二元复合物包括含有hTOPII核心结构域（hTOPIIcore）的hTOPII部分和与hTOPII部分复合的合成双链DNA。 合成双链DNA具有包含5'-NNNCCGAGCNNNNGCTCGGNNN-3'（SEQ ID NO：1）序列的3至20个核苷酸位置的第一个DNA链，其中N是腺嘌呤，胸腺嘧啶，胞嘧啶或鸟嘌呤中的任何一个 和与第一DNA链互补的第二DNA链。

公开(公告)号：US20070054267A1

公开(公告)日：2007-03-08

申请号：US10524860

申请日：2003-08-27

申请人： Takeshi Koizumi ,  Yoko Nishiyama ,  Satoshi Yamamoto ,  Masafumi Fukuyama ,  Katsunori Furuhata ,  Kenji Oonaka

发明人： Takeshi Koizumi ,  Yoko Nishiyama ,  Satoshi Yamamoto ,  Masafumi Fukuyama ,  Katsunori Furuhata ,  Kenji Oonaka

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/689 ,  C12N9/90 ,  C12Y599/01003 ,  Y02A50/451

摘要： To produce a high-performance specific gene amplification primer for detecting, quantifying, or identifying Vibrio vulnificus, having low risk of misidentification and practically sufficient amplification efficiency and amplification specificity. We have determined partial nucleotide sequences of gyrB, rpoD, and recA genes of Vibrio vulnificus and the closely related species, revealed their phylogenetic relationship, and then identified nucleotides characteristic to Vibrio vulnificus. Thus, we have made it possible to design a probe having high specificity and a gene amplification primer having high specificity and excellent amplification efficiency, both of which contain the characteristic nucleotides.

摘要翻译： 制备用于检测，定量或鉴定创伤弧菌的高性能特异性基因扩增引物，具有低错误识别风险和实际上足够的扩增效率和扩增特异性。 我们确定了创伤弧菌和密切相关物种的gyrB，rpoD和recA基因的部分核苷酸序列，揭示了其系统发育关系，然后确定了创伤弧菌特征的核苷酸序列。 因此，可以设计具有高特异度的探针和具有高特异性和优异的扩增效率的基因扩增引物，两者都含有特征性核苷酸。

公开(公告)号：US10000807B2

公开(公告)日：2018-06-19

申请号：US15254553

申请日：2016-09-01

申请人： Fenfei Leng ,  Xiaoduo Zhi ,  Samantha Dages ,  Kelley Dages

发明人： Fenfei Leng ,  Xiaoduo Zhi ,  Samantha Dages ,  Kelley Dages

IPC分类号： C12Q1/6876 ,  C12N15/70 ,  C12N9/90 ,  C12Q1/66 ,  C12Q1/68

CPC分类号： C12Q1/6876 ,  C12N9/90 ,  C12N15/1086 ,  C12N15/63 ,  C12N15/70 ,  C12Q1/025 ,  C12Q1/533 ,  C12Q1/66 ,  C12Q2600/158 ,  C12Y599/01003 ,  G01N33/5023

摘要： The disclosure describes the effects of transcription mediated from a promoter on the transcription mediated by divergently coupled supercoiling-sensitive promoter. Transcription initiated from a promoter inhibits transcription mediated by a specific supercoiling-sensitive promoter that is divergently coupled to the promoter. A gyrase inhibitor relieves this inhibition and substantially increases the transcription mediated by the specific supercoiling-sensitive promoter that is divergently coupled to another promoter. Accordingly, the invention pertains to a method for identifying a compound as a gyrase inhibitor or not a gyrase inhibitor based on differential expression of genes under the control of divergently coupled promoters in the presence of the compound. Another embodiment of the invention provides an assay for identifying one or more compounds from a library of compounds as a gyrase inhibitor. Polynucleotides and cells containing such polynucleotides that are suitable for carrying out the methods described herein are also provided.