公开(公告)号：US20160265028A1

公开(公告)日：2016-09-15

申请号：US15032541

申请日：2014-10-28

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Kyung KIM ,  Nakwon CHOI ,  Dong Jin LEE ,  Seungwon JUNG

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01J2219/00317 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01L3/50851 ,  B01L2200/16 ,  B01L2300/069 ,  B01L2300/0819 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/519

Abstract: A porous structure according to the present invention has a polymerase chain reaction (PCT) primer inside pores thereof, and hence, even an inner portion thereof can be used unlike general structures of which only surfaces are used for amplification and detection, thereby maximizing reactivity. In addition, the differentiating of the kinds of primers contained in respective structures leads to detection of several kinds of target nucleic acids at the same and real-time analysis thereof at the same time, and thus is useful for multiplex real-time PCR.

Abstract translation: 根据本发明的多孔结构在其孔内具有聚合酶链式反应（PCT）引物，因此，与其仅使用表面用于扩增和检测的一般结构相比，甚至可以使用其内部部分，从而使反应性最大化。 此外，各结构中所含的引物种类的差异导致同时检测多种目标核酸并进行实时分析，因此可用于多重实时PCR。

公开(公告)号：US20160263577A1

公开(公告)日：2016-09-15

申请号：US15022879

申请日：2014-09-18

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  SLIPCHIP CORPORATION

Inventor： Rustem F. ISMAGILOV ,  Feng SHEN ,  Liang LI ,  David SELCK ,  Joe BAKER ,  Espir KAHATT ,  Chris DA COSTA

IPC: B01L3/00 ,  G01N1/40

CPC classification number: B01L3/527 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/523 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/041 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/045 ,  B01L2300/046 ,  B01L2300/047 ,  B01L2300/0672 ,  B01L2300/069 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2300/161 ,  B01L2300/1855 ,  B01L2300/1877 ,  B01L2400/0409 ,  B01L2400/043 ,  B01L2400/0457 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0493 ,  B01L2400/0644 ,  B01L2400/0677 ,  B01L2400/0683 ,  G01N1/4077

Abstract: The present invention relates to fluidic systems for controlling one or more fluids and/or one or more reagents. These systems can be used in combination with one or more devices for assaying, processing, and/or storing samples. In particular, the systems and related methods can allow for dispensing fluid in a controlled manner and/or introducing pause(s) when implementing assays or processes.

Abstract translation: 本发明涉及用于控制一种或多种流体和/或一种或多种试剂的流体系统。 这些系统可以与用于测定，处理和/或存储样品的一个或多个装置组合使用。 特别地，系统和相关方法可以允许以受控的方式分配流体和/或在实施测定或过程时引入暂停。

公开(公告)号：US20160238595A1

公开(公告)日：2016-08-18

申请号：US15030232

申请日：2014-10-21

Applicant: The Regents of the University of California

Inventor： Amy E. Herr ,  Robert Lin

IPC: G01N33/543 ,  G01N27/447 ,  G01N33/68 ,  B01L3/00 ,  G01N33/545 ,  G01N33/561

CPC classification number: G01N33/54366 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/12 ,  B01L2300/0627 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/12 ,  B01L2400/0421 ,  G01N27/44726 ,  G01N27/44791 ,  G01N33/54353 ,  G01N33/545 ,  G01N33/561 ,  G01N33/6845

Abstract: Microfluidic devices for determining whether an analyte is present in a sample are provided. The microfluidic devices include a polymeric medium that includes a first analyte detection domain having a first covalently bound capture member that specifically binds to a first analyte, and a second analyte detection domain having a second covalently bound capture member that specifically binds to a second analyte. Also provided are methods of using the subject microfluidic device, systems and kits that use the subject microfluidic devices, as well as methods of producing the same.

Abstract translation: 提供用于确定样品中是否存在分析物的微流体装置。 微流体装置包括聚合物介质，其包括具有特异性结合第一分析物的第一共价结合捕获构件的第一分析物检测区域和具有特异性结合第二分析物的第二共价结合捕获构件的第二分析物检测区域。 还提供了使用本发明的微流体装置，使用本发明的微流体装置的系统和试剂盒的方法以及其制备方法。

公开(公告)号：US20160178625A1

公开(公告)日：2016-06-23

申请号：US15006153

申请日：2016-01-26

Applicant: EMD Millipore Corporation

Inventor： Chris Scott ,  Phillip Clark ,  Kurt E. Greenizen ,  Ryan A. Amara ,  Robert Colonna

IPC: G01N33/558 ,  B01L9/00 ,  B01L3/00

CPC classification number: G01N33/558 ,  B01J20/28033 ,  B01J20/28035 ,  B01J20/2804 ,  B01L3/5023 ,  B01L9/52 ,  B01L2200/025 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0809 ,  B01L2300/12 ,  B01L2300/126 ,  B01L2400/0487 ,  B01L2400/049 ,  G01N33/54366 ,  G01N33/56911 ,  G01N2469/10 ,  G01N2469/20

Abstract: The embodiments disclosed herein are directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.

Abstract translation: 本文公开的实施方案涉及用于在印迹膜上进行化合物检测的装置。 该装置包括多个层，包括印迹膜下面的多孔支撑层，印迹膜上方的流量分布器，以及任选的流量分配器上的孔，以将液体包含到期望的区域并允许 降低这种液体的起始体积。 优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜，并且支撑层是格栅或烧结的多孔材料。 分配器和支撑件保持在一起以在膜周围形成一个信封。 在这样做时优选使用铰链，夹子和其它这样的装置。

公开(公告)号：US09370732B2

公开(公告)日：2016-06-21

申请号：US14181656

申请日：2014-02-15

Applicant: Douglas T. Gjerde

Inventor： Douglas T. Gjerde

IPC: G01N1/34 ,  B01D15/38 ,  G01N1/40 ,  G01N15/00 ,  G01N15/10 ,  G01N35/04 ,  G01N15/14 ,  B01L3/02 ,  G01N35/10

CPC classification number: B01D15/22 ,  B01D15/14 ,  B01D15/3809 ,  B01D15/424 ,  B01L3/0275 ,  B01L2200/0647 ,  B01L2300/069 ,  G01N1/34 ,  G01N1/405 ,  G01N15/1463 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2035/0434 ,  G01N2035/1053

Abstract: This invention relates to devices and methods for purifying biological cells. For example, viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a pipette tip column. Methods of the invention can aid research, diagnosis and treatment of cancer.

Abstract translation: 本发明涉及用于纯化生物细胞的装置和方法。 例如，可以使用移液管尖端柱从复杂的生物样品中纯化可行的肿瘤，茎，免疫和精子细胞。 本发明的方法可以帮助癌症的研究，诊断和治疗。

公开(公告)号：US09366631B2

公开(公告)日：2016-06-14

申请号：US13191952

申请日：2011-07-27

Applicant: Eugene Tan ,  Heung Chuan Lam ,  Valery Leonidovich Bogdanov ,  Gregory John Kellogg ,  John A. Wright ,  Ulrich Hans Thomann ,  Richard F. Selden

Inventor： Eugene Tan ,  Heung Chuan Lam ,  Valery Leonidovich Bogdanov ,  Gregory John Kellogg ,  John A. Wright ,  Ulrich Hans Thomann ,  Richard F. Selden

IPC: G01N21/64 ,  B01L3/00 ,  G01N27/447 ,  B01L7/00

CPC classification number: B01L7/52 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/16 ,  B01L2300/1822 ,  B01L2300/1844 ,  B01L2300/1894 ,  B01L2400/0421 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N21/6402 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6486 ,  G01N27/44726 ,  G01N27/44743 ,  G01N27/44782 ,  G01N27/44791 ,  G01N2021/6441 ,  G01N2201/06113 ,  Y10T436/2575

Abstract: The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.

Abstract translation: 本公开提供了完全集成的微流体系统以进行核酸分析。 这些过程包括样品收集，核酸提取和纯化，扩增，测序和分离和检测。 本公开还提供了用于分离和检测生物分子的光学检测系统和方法。 特别地，本发明的各个方面能够在一个或多个微流体室或通道内同时分离和检测多个生物分子，通常是荧光染料标记的核酸。 核酸可以用至少6种染料进行标记，每种染料具有独特的峰值发射波长。 本发明的系统和方法对于DNA片段上浆应用尤其有用，例如通过遗传指纹图谱和DNA测序应用进行人类鉴定，如临床诊断。

公开(公告)号：US09365019B2

公开(公告)日：2016-06-14

申请号：US14312061

申请日：2014-06-23

Applicant: Xerox Corporation

Inventor： Jing Zhou ,  Mandakini Kanungo ,  Nancy Y. Jia ,  Wei Hong

IPC: B32B37/00 ,  B05C11/00 ,  B32B37/06 ,  B32B37/04 ,  B32B37/10 ,  B32B37/18 ,  B30B15/06

CPC classification number: B32B37/0046 ,  B01L3/5023 ,  B01L2200/12 ,  B01L2300/069 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/126 ,  B01L2300/161 ,  B05C11/00 ,  B30B15/062 ,  B30B15/064 ,  B32B37/04 ,  B32B37/06 ,  B32B37/10 ,  B32B37/18 ,  B32B2307/728 ,  B32B2307/73 ,  B32B2309/02 ,  B32B2535/00

Abstract: An apparatus for distributing a hydrophobic material in a hydrophilic substrate includes a first plate, a second plate, a heater operatively connected to the first plate and configured to heat the first plate to a first temperature that is greater than a second temperature of the second plate, and an actuator configured to move the first plate and second plate together with a hydrophilic substrate between the plates. The first plate and second plate form a temperature gradient to enable a hydrophobic material on one side of a hydrophilic substrate that faces the second plate to penetrate into the hydrophilic substrate towards the first plate.

Abstract translation: 用于在亲水基底中分配疏水材料的装置包括第一板，第二板，加热器，其可操作地连接到第一板，并且被配置为将第一板加热到大于第二板的第二温度的第一温度 以及构造成将第一板和第二板与亲水基板一起移动到板之间的致动器。 第一板和第二板形成温度梯度，以使亲水基底的面向第二板的一侧上的疏水材料朝向第一板渗入亲水基底。

公开(公告)号：US09346046B2

公开(公告)日：2016-05-24

申请号：US14379132

申请日：2013-03-05

Applicant: Porex Corporation

Inventor： Edward Jino Kim ,  Christopher Lynch ,  Shivananada Patrick ,  Guoqiang Mao

IPC: B01L3/02 ,  B23P19/04

CPC classification number: B01L3/0275 ,  B01L2200/025 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/141 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0832 ,  B01L2400/0672 ,  B23P19/04 ,  Y10T29/49945

Abstract: The present invention provides sintered porous plastic plugs for use in filtered serological pipettes which facilitate manufacture or assembly of the filtered serological pipettes. In one embodiment, a sintered porous plastic plug of the present invention comprises plastic and a cylindrical body, a first end and a second end, the first end having a cross-sectional area less than the cross-sectional area of the cylindrical body, and the second end having a cross-sectional area less than the cross-sectional area of the cylindrical body.

Abstract translation: 本发明提供用于过滤血清移液管的烧结多孔塑料塞，其有助于过滤血清移液管的制造或装配。 在一个实施例中，本发明的烧结多孔塑料塞包括塑料和圆筒体，第一端和第二端，第一端的横截面面积小于圆柱体的横截面面积;以及 所述第二端的横截面面积小于所述圆柱体的横截面面积。

公开(公告)号：US20160136644A1

公开(公告)日：2016-05-19

申请号：US14940662

申请日：2015-11-13

Applicant: Seiko Epson Corporation

Inventor： Kotaro IDEGAMI ,  Masato HANAMURA

IPC: B01L3/00 ,  B65D77/04 ,  C12Q1/68 ,  B65D65/38

CPC classification number: B01L3/52 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/142 ,  B01L2200/143 ,  B01L2200/16 ,  B01L2300/069 ,  B01L2300/0832 ,  B01L2300/087 ,  B01L2400/043 ,  B32B1/02 ,  B32B15/08 ,  B32B15/20 ,  B32B27/08 ,  B32B27/306 ,  B32B27/32 ,  B32B27/36 ,  B32B2255/10 ,  B32B2255/20 ,  B32B2255/205 ,  B32B2307/724 ,  B32B2307/7246 ,  B32B2307/7265 ,  B32B2439/40 ,  B32B2439/46 ,  B65D81/22 ,  B65D81/268

Abstract: A container storage receptacle includes a package, and a cleaning container that is enclosed and stored in the package, and that encloses and stores a cleaning solution for cleaning a nucleic acid binding activity solid-phase carrier which has an adsorbed nucleic acid. The cleaning solution contains alcohol, and an inner portion of the package is in a state of being saturated with alcohol vapor.

Abstract translation: 容器储存容器包括一个封装和一个封闭并存放在包装中的清洁容器，并且包围并存储一个用于清洗具有吸附核酸的核酸结合活性固相载体的清洁溶液。 清洁溶液含有醇，并且包装的内部部分处于被酒精蒸气饱和的状态。

公开(公告)号：US09341624B2

公开(公告)日：2016-05-17

申请号：US13930628

申请日：2013-06-28

Applicant: Invisible Sentinel, Inc.

Inventor： Nicholas A. Siciliano ,  Martin Joseph Bouliane

IPC: G01N33/558 ,  G01N33/569 ,  B01L3/00 ,  G01N33/53

CPC classification number: G01N33/5302 ,  B01L3/5023 ,  B01L2200/026 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2300/041 ,  B01L2300/049 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0809 ,  B01L2300/087 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56922 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/804 ,  Y10S436/805 ,  Y10S436/807

Abstract: Devices and methods for the detection of target molecules are disclosed. Devices and methods for detecting food-borne target molecules are also disclosed.