公开(公告)号：US12234573B2

公开(公告)日：2025-02-25

申请号：US17506733

申请日：2021-10-21

Applicant: CHUGAI SEIYAKU KABUSHIKI KAISHA

Inventor： Tomoyuki Igawa ,  Shojiro Kadono ,  Naoka Hironiwa ,  Mika Sakurai

IPC: C40B40/10 ,  A61K39/395 ,  C07K16/28 ,  C07K16/42 ,  C12N15/09 ,  C40B30/04 ,  C40B40/08

Abstract: The present inventors have successfully prepared a library consisting essentially of a plurality of antigen-binding molecules differing in sequence from each other, the antigen-binding molecules each comprising an antibody variable region that has binding activity against a first antigen and a second antigen different from the first antigen, but does not bind to the first antigen and the second antigen at the same time. Use of the library of the present invention allows the obtainment of a variable region having enhanced ability to bind to the first antigen and the production of a bispecific antibody against the first antigen and a cancer antigen. Moreover, the present inventors have also successfully prepared an antigen-binding molecule comprising an antibody variable region that has binding activity against three different antigens, but does not bind to these antigens at the same time.

公开(公告)号：US20250061962A1

公开(公告)日：2025-02-20

申请号：US18617186

申请日：2024-03-26

Applicant: TRIPLEBAR BIO, INC.

Inventor： Jeremy AGRESTI ,  Andres ORNELAS VARGAS ,  Kevin Gregory HOFF

IPC: G16B20/00 ,  C12N15/10 ,  C40B30/04 ,  G16B20/50 ,  G16B35/10 ,  G16B40/00 ,  G16B99/00

Abstract: Provided herein are systems and methods for screening desirable biological variants using a high-throughput integrated system. The integrated system may be configured to input a plurality of parameters from functional studies of biological variants under applied conditions, in conjunction with integrated libraries of biological variants, and filter the inputs to produce desirable biological variants based on an input performance requirement. The system may output optimized strains, molecules, or novel molecules expected to have a desirable functional characteristic. Accordingly, the methods and systems disclosed herein enable multi-parametric studies of biological diversity and conditional diversity in systems biology.

公开(公告)号：US12227737B2

公开(公告)日：2025-02-18

申请号：US18061273

申请日：2022-12-02

Applicant: University Health Network ,  Sinai Health System

Inventor： Daniel Diniz De Carvalho ,  Shu Yi Shen ,  Rajat Singhania ,  Scott Victor Bratman ,  Ankur Ravinarayana Chakravarthy

IPC: C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6886 ,  C40B30/04 ,  G01N33/53

Abstract: There is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 mg of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.

公开(公告)号：US12181480B2

公开(公告)日：2024-12-31

申请号：US17111403

申请日：2020-12-03

Applicant: Adimab, LLC

Inventor： Piotr Bobrowicz ,  Amber D. Hanna ,  Jerry M. Thomas

IPC: C40B30/04 ,  C12N15/10 ,  G01N33/68

Abstract: The present invention relates, inter alia, to polyspecificity reagents, methods of making the same, and methods of using the same in, inter alia, the selection, screening, enrichment, and identification of non-polyspecific, and thus developable, polypeptides.

公开(公告)号：US12146134B2

公开(公告)日：2024-11-19

申请号：US15996222

申请日：2018-06-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC: C12N15/10 ,  B01F33/302 ,  B01F33/3031 ,  B01J19/00 ,  B01L3/00 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/04 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6876 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N33/536 ,  G01N33/543 ,  G01N33/68 ,  B01F23/41 ,  B01L7/00 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/6869 ,  G01N21/64 ,  G01N33/542

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

公开(公告)号：US12139815B2

公开(公告)日：2024-11-12

申请号：US17586687

申请日：2022-01-27

Applicant: Lawrence Livermore National Security, LLC

Inventor： Magdalena Franco ,  Nicole Collette ,  Patrik D'Haeseleer

IPC: C40B30/04 ,  C40B40/10 ,  G16B20/30 ,  G16B40/20

Abstract: A newly developed generalizable screening method enables efficient identification of epitopes, in the proteomes of pathogenic agent is provided. Also disclosed are systems that enable the method as well as epitopes discovered using the method.

公开(公告)号：US20240344241A1

公开(公告)日：2024-10-17

申请号：US18701094

申请日：2022-10-14

Applicant: THE UNIVERSITY OF QUEENSLAND ,  The University of Tokyo

Inventor： David James CRAIK ,  Simon John DE VEER ,  Yen-Hua HUANG ,  Joakim Erik SWEDBERG ,  Hiroaki SUGA ,  Wenyu LIU ,  Toby PASSIOURA

IPC: C40B30/04 ,  C12N15/10 ,  C12Q1/02 ,  C12R1/19 ,  G01N33/68

CPC classification number: C40B30/04 ,  C12N15/1062 ,  C12Q1/02 ,  G01N33/6845 ,  C12R2001/19 ,  G01N2500/04

Abstract: Disclosed are proteinaceous coagulation factor XIIa (FXIIa) inhibitors and their use for treating or inhibiting the development of a condition in which inhibiting FXIIa stimulates or effects treatment or inhibition of the development of the condition. Suitable conditions include thromboembolism-associated conditions such as acute coronary syndrome, stroke, deep vein thrombosis and pulmonary embolism, a thrombosis, a thrombosis-associated hematologic disorder such as sickle cell disease or thrombophilia, and an inflammatory condition or a condition related to the kallikrein-kinin system such as hereditary angioedema, multiple sclerosis, rheumatoid arthritis or lupus. The proteinaceous FXIIa inhibitors are also useful for treating or inhibiting thrombus and/or embolus formation. In vitro methods for identifying a disulfide rich peptide which binds to a target substance are also disclosed.

公开(公告)号：US12110611B2

公开(公告)日：2024-10-08

申请号：US17573389

申请日：2022-01-11

Applicant: BIOATLA, INC.

Inventor： Jay M. Short ,  Hwai Wen Chang ,  Gerhard Frey

IPC: C40B30/04 ,  C07K16/00 ,  C07K16/28 ,  C07K16/30 ,  C12N15/10 ,  C40B40/08 ,  C40B40/10

CPC classification number: C40B30/04 ,  C07K16/00 ,  C07K16/005 ,  C07K16/2803 ,  C07K16/2809 ,  C07K16/30 ,  C12N15/1058 ,  C12N15/1093 ,  C40B40/08 ,  C40B40/10 ,  C07K2317/14 ,  C07K2317/31 ,  C07K2317/56 ,  C07K2317/90 ,  C07K2317/94

Abstract: Methods of generating conditionally active biologic proteins, in particular therapeutic or diagnostic proteins, which are more active at an aberrant condition than at a normal physiological condition. The methods include discovery methods using libraries of proteins and assays employing physiological concentrations of components of bodily fluids. The conditionally active biologic proteins may be further evolved, conjugated to other molecules, masked, reduced in activity by attaching a cleavable moiety. Criteria for selecting starting proteins for the discovery methods, as well as formats of the proteins are also disclosed.

公开(公告)号：US12092635B2

公开(公告)日：2024-09-17

申请号：US18386066

申请日：2023-11-01

Applicant: 10X GENOMICS, INC.

Inventor： Tarjei Sigurd Mikkelsen ,  Eswar Prasad Ramachandran Iyer ,  Andrew Kohlway ,  Luigi Jhon Alvarado Martinez ,  Katherine Pfeiffer ,  Andrew D. Price

IPC: G01N33/532 ,  C07K14/74 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C40B30/04 ,  C40B50/06 ,  C40B70/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/548 ,  G01N33/569 ,  G01N33/58

CPC classification number: G01N33/532 ,  C07K14/70539 ,  C12N15/1037 ,  C12N15/1055 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/5032 ,  G01N33/505 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/5308 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/548 ,  G01N33/56977 ,  G01N33/58 ,  C12N2310/20 ,  C12N2320/10 ,  C12Q2537/164 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/1015 ,  C40B30/04 ,  C40B50/06 ,  C40B70/00

Abstract: The present disclosure provides methods of processing or analyzing a sample. A method for processing a sample may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule), barcoding the probe-nucleic acid molecule complex, and performing extension, denaturation, and amplification processes. A method for processing a sample may comprise hybridizing first and second probes to adjacent or non-adjacent target regions of a nucleic acid molecule (e.g., an RNA molecule), linking the first and second probes to provide a probe-linked nucleic acid molecule, and barcoding the probe-linked nucleic acid molecule. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well. One or more processes of the methods described herein may be performed on a cell, such as a permeabilized cell.

公开(公告)号：US20240279642A1

公开(公告)日：2024-08-22

申请号：US18570580

申请日：2022-06-14

Applicant: Protillion Biosciences, Inc.

Inventor： Curtis James LAYTON ,  Pavanapuresan Pushpagiri VAIDYANATHAN ,  Michael Roy GOTRIK

IPC: C12N15/10 ,  C40B30/04 ,  C40B40/08 ,  G16B35/20

CPC classification number: C12N15/1062 ,  C40B30/04 ,  C40B40/08 ,  G16B35/20

Abstract: Methods, systems, and composition for the analysis of polypeptides and the generation of polypeptide libraries are disclosed. Analysis of polypeptide library may be used to generate polypeptide with particular characteristics. Antibodies with high affinities may be generated using the methods, systems and compositions disclosed.