公开(公告)号：US06815217B2

公开(公告)日：2004-11-09

申请号：US09463547

申请日：2000-01-21

申请人： Johann Karl ,  Helmut Lenz ,  Friedemann Krause ,  Peter Finckh ,  Hans Hornauer ,  Johann Berger

发明人： Johann Karl ,  Helmut Lenz ,  Friedemann Krause ,  Peter Finckh ,  Hans Hornauer ,  Johann Berger

IPC分类号： G01N33553

CPC分类号： G01N33/54366 ,  C12Q1/6834 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/973 ,  Y10S435/974

摘要： A solid phase with at least one test area is described which contains reagents for the detection of at least one analyte in a sample, wherein the solid phase additionally comprises at least one control area for the detection of interfering reactions.

摘要翻译： 描述了具有至少一个测试区域的固相，其包含用于检测样品中至少一种分析物的试剂，其中所述固相还包含用于检测干扰反应的至少一个控制区域。

公开(公告)号：US06770489B1

公开(公告)日：2004-08-03

申请号：US09621341

申请日：2000-07-21

申请人： Keiji Enpuku

发明人： Keiji Enpuku

IPC分类号： G01N33553

CPC分类号： G01N33/54326

摘要： The present invention relates to an immunoassay and an apparatus for the same. A method of the present invention comprises following processes: (1) an analyte is labeled to detect antigen-antibody reaction, (2) the magnetic material label is magnetized by a magnetic field, (3) the magnetized magnetic material label detected by a SQUID which detect a magnetic field having right angle to the magnetic field. At the same time, the present invention contains an apparatus to execute the method provided by the present invention. The apparatus comprises a magnetic field generation means that generates a magnetic field to magnetize the labels. The apparatus comprises a SQUID that measures magnetic field.

摘要翻译： 本发明涉及一种免疫测定及其装置。 本发明的方法包括以下过程：（1）标记分析物以检测抗原 - 抗体反应，（2）磁性材料标记被磁场磁化，（3）由SQUID检测到的磁化磁性材料标签 其检测与磁场成直角的磁场。 同时，本发明包含执行本发明提供的方法的装置。 该装置包括产生磁场来磁化标签的磁场产生装置。 该装置包括测量磁场的SQUID。

公开(公告)号：US06696304B1

公开(公告)日：2004-02-24

申请号：US09512013

申请日：2000-02-24

申请人： Travis Parker Davies

发明人： Travis Parker Davies

IPC分类号： G01N33553

CPC分类号： G01N33/54346 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/105831

摘要： A process, a test kit, calibration standards, and the method of preparing such standards as useful for the qualitative and/or quantitative determination of total solid phase- or microparticle-immobilized, amine-containing reactants such as proteins is provided. The operating principle of the invention is distinct from a classical immunoassay based on antibody-antigen immune interaction or from standard calorimetric proteins assays of soluble proteins and allows the detection of as low as attogram (10−18 gm) levels of total analyte per microparticle.

摘要翻译： 提供了一种方法，测试试剂盒，校准标准品和制备用于定量和/或定量测定总固相或微粒固定的含胺反应物如蛋白质的标准品的方法。 本发明的工作原理不同于基于抗体 - 抗原免疫相互作用的经典免疫测定法或来自可溶性蛋白质的标准量热蛋白测定法，并且允许检测到低至至少（10 -18）gm水平的总分析物 微粒。

公开(公告)号：US06548309B1

公开(公告)日：2003-04-15

申请号：US09044677

申请日：1998-03-19

申请人： Norman James Moore ,  Vincent Anthony Sy

发明人： Norman James Moore ,  Vincent Anthony Sy

IPC分类号： G01N33553

CPC分类号： G01N1/4077 ,  B01L3/5023 ,  B01L9/54 ,  B01L2300/0681 ,  B01L2300/0825 ,  G01N1/34 ,  Y10S436/808 ,  Y10S436/814 ,  Y10S436/818 ,  Y10S436/825

摘要： In qualitative or quantitative assays which employ a test device comprising a test strip pretreated for detection of a specified target analyte dissolved in a liquid medium, the presence of solid, semisolid and/or colloidal materials in the liquid medium may interfere with the assay results. The present invention involves collecting the sample from a flowing or quiescent pool of liquid also containing solid, semisolid or colloidal material with a swab comprised of a handle and a mass of fibrous material or foamed, open cell material which, when immersed in and thoroughly wetted by the liquid, entraps solid, semisolid and/or colloidal material and delivers only the liquid to the sample receiving zone of the test strip.

摘要翻译： 在采用包含预处理以检测溶解在液体介质中的特定目标分析物的试纸的测试装置的定性或定量测定中，液体介质中固体，半固体和/或胶体材料的存在可能干扰测定结果。 本发明涉及从具有固体，半固体或胶体材料的流动或静止液体的液体池中收集样品，其中包括手柄和一批纤维材料或泡沫的开孔细胞材料，当浸入并充分润湿 通过液体，捕获固体，半固体和/或胶体材料，并仅将液体输送到测试条的样品接收区。

公开(公告)号：US06468810B1

公开(公告)日：2002-10-22

申请号：US09600816

申请日：2000-07-24

申请人： Matti Korpela

发明人： Matti Korpela

IPC分类号： G01N33553

CPC分类号： G01N35/10 ,  B03C1/284 ,  G01N33/54326 ,  G01N35/0098

摘要： A device for transfer suitable for capturing and releasing microparticles binding an immobilized substance, which includes a magnet as well as either an extendable membrane, shapable membrane or magnet's coating such that the membrane or coating pressing tightly against the magnet's surface separates the magnet from the microparticles but does not substantially weaken the magnetic field directed at the microparticles. Also disclosed is a method for transferring a microparticle immobilized substance from a first vessel to a second vessel where the microparticles are of a magnetic or magnetizable material or the microparticles are attached to a magnetic or magnetizable body and the microparticles with the substance immobilized thereupon are captured with the aid of a magnet submerged in the first vessel, the magnet along with the microparticles captured thereupon are transferred to the second vessel and released from the magnet's influence.

摘要翻译： 一种用于捕获和释放结合固定化物质的微粒的转移装置，其包括磁体以及可延伸膜，可成形膜或磁体的涂层，使得紧密抵靠磁体表面的膜或涂层将磁体与微粒分离 但是基本上不会弱化指向微粒的磁场。 还公开了一种将微粒固定物质从第一容器转移到第二容器的方法，其中微粒是磁性或可磁化材料，或者微粒附着到磁性或可磁化体上，并且捕获固定有物质的微粒被捕获 借助于淹没在第一容器中的磁体，将磁体与其上捕获的微粒一起转移到第二容器并从磁体的影响中释放。

公开(公告)号：US06455324B1

公开(公告)日：2002-09-24

申请号：US09658000

申请日：2000-09-08

申请人： Joel S. Douglas

发明人： Joel S. Douglas

IPC分类号： G01N33553

CPC分类号： G01N33/525 ,  B01L3/5023 ,  B01L3/5027 ,  B01L2200/0605 ,  B01L2200/0689 ,  B01L2300/069 ,  B01L2300/0825 ,  B01L2300/0887 ,  B01L2400/0406 ,  C12Q1/54 ,  G01N33/66 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/805 ,  Y10S436/808 ,  Y10S436/809 ,  Y10S436/81 ,  Y10S436/823 ,  Y10T436/117497

摘要： The invention provides a method for measuring the amount of analyte in a sample of biological fluid using a simple low sample volume reagent test strip with a built in metering system. The test strip may comprise a microtitration zone to prevent oversampling and an integrated capillary to prevent problems associated with short sampling and act as means of absorbing the fluid sample. The test strip comprises a wicking layer and a reaction matrix embossed layer in the form of a pillow assembled into a microtitration pocket formed in the strip. The test strip is used in single use applications such as the determination of the concentration of glucose in blood.

摘要翻译： 本发明提供一种使用具有内置计量系统的简单的低样本体积试剂测试条来测量生物流体样品中分析物的量的方法。 测试条可以包括微量滴定区以防止过采样和集成毛细管以防止与短取样相关的问题，并且作为吸收流体样品的手段。 测试条包括芯组形式的芯吸层和反应基质压花层，所述枕头组装成形成在条带中的微量滴定袋。 测试条用于单次使用应用中，例如测定血液中葡萄糖的浓度。

公开(公告)号：US06432630B1

公开(公告)日：2002-08-13

申请号：US09254310

申请日：1999-12-16

申请人： Gert Blankenstein

发明人： Gert Blankenstein

IPC分类号： G01N33553

CPC分类号： B03C5/028 ,  B01D57/02 ,  B01J19/0093 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/084 ,  B03C1/035 ,  B03C5/026 ,  G01N15/1031 ,  G01N15/1056 ,  G01N15/1484 ,  G01N33/56966 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/149

摘要： A micro flow system is provided for separating particles, comprising a microfabricated member having a flow channel (5) defined therein for guiding a flow of a fluid containing the particles through the flow channel, first inlet means (2) positioned at one end of the flow channel for entering the fluid into the flow channel, first outlet means (7) positioned at the other end of the flow channel for discharging the fluid from the flow channel, the flow of the fluid containing the particles being controlled in such a way that one particle at the time passes a cross section of the flow channel, the member being positioned in a field that is substantially perpendicular to a longitudinal axis of the flow channel so that particles residing in the flow channel and being susceptible to the filed across the flow channel are deflected in the direction of the field. Further, a micro flow system is provided for analyzing components of a fluid comprising a microfabricated member having a flow channel defined herein for guiding a flow of a fluid from the flow channel, first inlet means for entering particles into the flow channel, first outlet means for discharging of fluid from the flow channel and a plurality of assay sites located in the flow channel and comprising immobilized reagents whereby the fluid may be analyzed for a plurality of components while residing in the flow channel.

摘要翻译： 提供微流量系统用于分离颗粒，包括微型构件，其具有限定在其中的流动通道（5），用于引导包含颗粒的流体通过流动通道，第一入口装置（2）位于 用于将流体进入流动通道的流动通道，位于流动通道的另一端的第一出口装置（7），用于从流动通道排出流体，包含颗粒的流体的流动被控制为使得 一个颗粒此时通过流动通道的横截面，该构件定位在基本上垂直于流动通道的纵向轴线的场中，使得位于流动通道中的颗粒易于流过流动的场 通道沿场的方向偏转。 此外，提供了一种微流量系统，用于分析包括具有本文定义的流动通道的微加速构件的流体的部件，用于引导来自流动通道的流体流动，用于将颗粒输入到流动通道中的第一入口装置，第一出口装置 用于从流动通道排放流体和位于流动通道中的多个测定位点，并且包括固定的试剂，由此可以在流体在流动通道中同时分析多种组分。

公开(公告)号：US06399397B1

公开(公告)日：2002-06-04

申请号：US09690823

申请日：2000-10-18

申请人： David A. Zarling ,  Michel J. Rossi ,  Norman A. Peppers ,  James Kane ,  Gregory W. Faris ,  Mark J. Dyer ,  Steve Y. Ng ,  Luke V. Schneider

发明人： David A. Zarling ,  Michel J. Rossi ,  Norman A. Peppers ,  James Kane ,  Gregory W. Faris ,  Mark J. Dyer ,  Steve Y. Ng ,  Luke V. Schneider

IPC分类号： G01N33553

CPC分类号： B82Y15/00 ,  B01J2219/00315 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00707 ,  C12Q1/6816 ,  C12Q1/6818 ,  C40B60/14 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/533 ,  G01N33/582 ,  G01N33/588 ,  G01N2021/6419 ,  G01N2021/6421 ,  Y10S435/97 ,  Y10S435/973 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/25375 ,  C12Q2563/00 ,  C12Q2563/107 ,  C12Q2537/143

摘要： The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. The methods, compositions, and apparatus are suitable for the sensitive detection of multiple analytes and for various clinical and environmental sampling techniques.

摘要翻译： 本发明提供了通过用上转换标记标记探针分子来进行分析物例如生物大分子和其它分析物的敏感检测的方法，组合物和装置。 上变换标签吸收来自照明源的辐射并以一个或多个更高频率发射辐射，提供增强的信噪比和基本消除背景样品自发荧光。 方法，组合物和装置适用于多种分析物的敏感检测和各种临床和环境取样技术。

公开(公告)号：US06274387B1

公开(公告)日：2001-08-14

申请号：US09220848

申请日：1998-12-28

申请人： Syoichi Yamauchi ,  Kiyoshi Kasai

发明人： Syoichi Yamauchi ,  Kiyoshi Kasai

IPC分类号： G01N33553

CPC分类号： B01J20/28016 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/28009 ,  B01J20/28057 ,  B01J20/28069 ,  B01J20/28078 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3257 ,  B01J20/3263 ,  B01J20/327 ,  B01J2220/54 ,  C12Q1/6806 ,  Y10S428/90 ,  Y10T428/2993

摘要： A novel magnetic carrier is provided which comprises particulate silica containing a magnetic material, having polyacrylamide on the surface thereof in an amount ranging from 0.3 to 5 mmol/g in terms of monomeric acrylamide. A process for producing the magnetic carrier is also provided in which the surface of particulate silica containing a magnetic material is treated with a coupling agent, and the treated particulate silica is reacted with acrylamide and/or polyacrylamide. The magnetic carrier is useful for extraction of nucleic acid. The magnetic carrier can be produced readily by controlling the shape, the particle diameter, and the pore diameter, and is excellent in strength and adsorption efficiency. The extraction of a nucleic acid can be automated by use of the magnetic carrier.

摘要翻译： 提供了一种新的磁性载体，其包含含有磁性材料的颗粒状二氧化硅，其表面上的聚丙烯酰胺的量为单体丙烯酰胺换算为0.3-5mmol / g。 还提供一种制备磁性载体的方法，其中用偶联剂处理含有磁性材料的颗粒状二氧化硅的表面，并将经处理的颗粒状二氧化硅与丙烯酰胺和/或聚丙烯酰胺反应。 磁性载体可用于提取核酸。 通过控制形状，粒径和孔径可以容易地制造磁性载体，并且强度和吸附效率优异。 可以通过使用磁性载体自动提取核酸。

公开(公告)号：US06207398B1

公开(公告)日：2001-03-27

申请号：US09372285

申请日：1999-08-11

申请人： Chengrong Wang

发明人： Chengrong Wang

IPC分类号： G01N33553

CPC分类号： G01N33/9493 ,  A61K39/00 ,  C07K7/645

摘要： The present invention provides novel cyclosporine C (CsC) derivatives having improved protein conjugatibility and hydrolytic stability. The present invention further provides a CsC derivative conjugated to a carrier, e.g., a solid support. Preferably, the solid support is a latex or magnetic particle.

摘要翻译： 本发明提供具有改善的蛋白质相容性和水解稳定性的新型环孢菌素C（CsC）衍生物。 本发明还提供了与载体例如固体载体缀合的CsC衍生物。 优选地，固体支持物是胶乳或磁性颗粒。