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31 条记录 (耗时 0.019 秒)

    Nanoscale array biomolecular bond enhancer device
    2.
    发明申请
    Nanoscale array biomolecular bond enhancer device 审中-公开

    公开(公告)号：US20080274916A1

    公开(公告)日：2008-11-06

    申请号：US12215239

    申请日：2008-06-26

    申请人： Patrick Denis Lincoln

    发明人： Patrick Denis Lincoln

    IPC分类号： C40B50/14

    CPC分类号： C07K1/047 ,  B82Y30/00 ,  Y10S977/789 ,  Y10S977/793 ,  Y10S977/902

    摘要： The invention teaches the use of an addressable nanoscale device to create a programmable substrate useful in selectively attracting proteins, nucleating protein crystals and growing protein crystals of a size amenable to diffraction analysis. Further taught is the use of nanoscale assemblies to create charge patterns, where such charge patterns are useful in purifying, nucleating or crystallizing protein molecules. Charge extension moieties, including water, are taught. The invention provides rapid and efficient identification, purification and detection of proteins and protein-related complexes.

    Immobilization of bioactive protein in phyllosilicates
    3.
    发明授权
    Immobilization of bioactive protein in phyllosilicates 失效
    生物活性蛋白在页硅酸盐中的固定

    公开(公告)号：US06180378B2

    公开(公告)日：2001-01-30

    申请号：US09239778

    申请日：1999-01-29

    申请人： Siyuan Shen ,  An-Fei Hsu ,  Thomas A. Foglia ,  Shu-I Tu

    发明人： Siyuan Shen ,  An-Fei Hsu ,  Thomas A. Foglia ,  Shu-I Tu

    IPC分类号： C12N1114

    CPC分类号： C12N9/0065 ,  C07K17/14 ,  C12N9/0069 ,  C12N11/14 ,  Y02P20/588 ,  Y10S530/811 ,  Y10S977/747 ,  Y10S977/793 ,  Y10S977/897 ,  Y10S977/898 ,  Y10S977/906

    摘要： Immobilized bioactive protein compositions are prepared containing a bioactive protein such as an enzyme intercalated into galleries of a phyllosilicate, and a crosslinking compound crosslinking the phyllosilicate and the bioactive protein. The phyllosilicate may contain sodium or alkylammonium ions and be montmorillonite. The protein may be lipoxygenase, and crosslinking compounds include tetramethyl orthosilicate, tetraethoxy silicate, propyltrimethoxy silicate, polydimethylortho silicate and methyltrimethoxy silicate. The composition is prepared by delaminating a sodium-saturated phyllosilicate, mixing a bioactive protein with the delaminated phyllosilicate and crosslinking with a crosslinking compound. After crosslinking, the composition may be vacuum dried and ground. The composition can also be prepared by delaminating a montmorillonite, saturating the delaminated montmorillonite with sodium ions, mixing the resultant montmorillonite with an enzyme, adding tetramethyl orthosilicate, allowing crosslinking, and drying. Activities of up to 170% of free protein are achieved using the immobilized bioactive protein compositions, and the compositions retain up to 98% original activity after being stored at room temperature for two weeks.

    摘要翻译： 制备含有生物活性蛋白质如插入到页硅酸盐的画廊中的酶以及交联页硅酸盐和生物活性蛋白质的交联化合物的固定的生物活性蛋白质组合物。 页硅酸盐可以含有钠或烷基铵离子并且是蒙脱石。 蛋白质可以是脂氧合酶，交联化合物包括原硅酸四甲酯，四乙氧基硅酸酯，丙基三甲氧基硅酸酯，聚二甲基硅氧烷和甲基三甲氧基硅酸酯。 通过使饱和的页硅酸盐分层，将生物活性蛋白质与分层的页硅酸盐混合并与交联化合物交联来制备组合物。 交联后，可将组合物真空干燥并研磨。 组合物也可以通过使蒙脱石分层，用钠离子饱和分解的蒙脱石，将所得蒙脱石与酶混合，加入原硅酸四甲酯，进行交联和干燥来制备。 使用固定的生物活性蛋白质组合物达到高达170％的游离蛋白质的活性，并且在室温下保存两周后，组合物保留高达98％的原始活性。

    Functionalized fluorescent nanocrystals, and methods for their preparation and use
    7.
    发明申请
    Functionalized fluorescent nanocrystals, and methods for their preparation and use 审中-公开

    公开(公告)号：US20080213919A1

    公开(公告)日：2008-09-04

    申请号：US12041589

    申请日：2008-03-03

    申请人： Imad Naasani

    发明人： Imad Naasani

    IPC分类号： G01N33/553 ,  B32B5/16 ,  B05D1/36 ,  G01N21/62

    CPC分类号： B82Y15/00 ,  C09K11/025 ,  C09K11/883 ,  G01N33/588 ,  Y10S977/773 ,  Y10S977/792 ,  Y10S977/793 ,  Y10S977/795 ,  Y10S977/811 ,  Y10S977/827 ,  Y10S977/83 ,  Y10T428/2991 ,  Y10T428/2993

    摘要： Functionalized fluorescent nanocrystal compositions and methods for making and using these compositions are disclosed. The compositions are fluorescent nanocrystals coated with at least one material. The coating material has chemical compounds or ligands with functional groups or moieties with conjugated electrons and moieties for imparting solubility to coated fluorescent nanocrystals in aqueous solutions. The coating material provides for functionalized fluorescent nanocrystal compositions which are water soluble, chemically stable, and emit light with a high quantum yield and/or luminescence efficiency when excited with light. The coating material may also have chemical compounds or ligands with moieties for bonding to target molecules and cells as well as moieties for cross-linking the coating. In the presence of reagents suitable for reacting to form capping layers, the compounds in the coating may form a capping layer on the fluorescent nanocrystal with the coating compounds operably bonded to the capping layer.

    Nanoscale array biomolecular bond enhancer device
    8.
    发明授权
    Nanoscale array biomolecular bond enhancer device 失效
    纳米阵列生物分子键增强剂装置

    公开(公告)号：US07417119B2

    公开(公告)日：2008-08-26

    申请号：US11333951

    申请日：2006-01-17

    申请人： Patrick Denis Lincoln

    发明人： Patrick Denis Lincoln

    IPC分类号： C07K1/00

    CPC分类号： C07K1/047 ,  B82Y30/00 ,  Y10S977/789 ,  Y10S977/793 ,  Y10S977/902

    摘要： The invention teaches the use of an addressable nanoscale device to create a programmable substrate useful in selectively attracting proteins, nucleating protein crystals and growing protein crystals of a size amenable to diffraction analysis. Further taught is the use of nanoscale assemblies to create charge patterns, where such charge patterns are useful in purifying, nucleating or crystallizing protein molecules. Charge extension moieties, including water, are taught. The invention provides rapid and efficient identification, purification and detection of proteins and protein-related complexes.

    摘要翻译： 本发明教导了使用可寻址纳米级装置来创建可用于选择性吸引蛋白质，成核蛋白质晶体和生长尺寸适于衍射分析的蛋白质晶体的可编程底物。 进一步教导的是使用纳米级组件来产生电荷模式，其中这种电荷模式可用于纯化，成核或结晶蛋白质分子。 讲授电荷延伸部分，包括水。 本发明提供蛋白质和蛋白质相关复合物的快速和有效的鉴定，纯化和检测。