公开(公告)号：US06180378B2

公开(公告)日：2001-01-30

申请号：US09239778

申请日：1999-01-29

申请人： Siyuan Shen ,  An-Fei Hsu ,  Thomas A. Foglia ,  Shu-I Tu

发明人： Siyuan Shen ,  An-Fei Hsu ,  Thomas A. Foglia ,  Shu-I Tu

IPC分类号： C12N1114

CPC分类号： C12N9/0065 ,  C07K17/14 ,  C12N9/0069 ,  C12N11/14 ,  Y02P20/588 ,  Y10S530/811 ,  Y10S977/747 ,  Y10S977/793 ,  Y10S977/897 ,  Y10S977/898 ,  Y10S977/906

摘要： Immobilized bioactive protein compositions are prepared containing a bioactive protein such as an enzyme intercalated into galleries of a phyllosilicate, and a crosslinking compound crosslinking the phyllosilicate and the bioactive protein. The phyllosilicate may contain sodium or alkylammonium ions and be montmorillonite. The protein may be lipoxygenase, and crosslinking compounds include tetramethyl orthosilicate, tetraethoxy silicate, propyltrimethoxy silicate, polydimethylortho silicate and methyltrimethoxy silicate. The composition is prepared by delaminating a sodium-saturated phyllosilicate, mixing a bioactive protein with the delaminated phyllosilicate and crosslinking with a crosslinking compound. After crosslinking, the composition may be vacuum dried and ground. The composition can also be prepared by delaminating a montmorillonite, saturating the delaminated montmorillonite with sodium ions, mixing the resultant montmorillonite with an enzyme, adding tetramethyl orthosilicate, allowing crosslinking, and drying. Activities of up to 170% of free protein are achieved using the immobilized bioactive protein compositions, and the compositions retain up to 98% original activity after being stored at room temperature for two weeks.

摘要翻译： 制备含有生物活性蛋白质如插入到页硅酸盐的画廊中的酶以及交联页硅酸盐和生物活性蛋白质的交联化合物的固定的生物活性蛋白质组合物。 页硅酸盐可以含有钠或烷基铵离子并且是蒙脱石。 蛋白质可以是脂氧合酶，交联化合物包括原硅酸四甲酯，四乙氧基硅酸酯，丙基三甲氧基硅酸酯，聚二甲基硅氧烷和甲基三甲氧基硅酸酯。 通过使饱和的页硅酸盐分层，将生物活性蛋白质与分层的页硅酸盐混合并与交联化合物交联来制备组合物。 交联后，可将组合物真空干燥并研磨。 组合物也可以通过使蒙脱石分层，用钠离子饱和分解的蒙脱石，将所得蒙脱石与酶混合，加入原硅酸四甲酯，进行交联和干燥来制备。 使用固定的生物活性蛋白质组合物达到高达170％的游离蛋白质的活性，并且在室温下保存两周后，组合物保留高达98％的原始活性。