公开(公告)号：US20160265022A1

公开(公告)日：2016-09-15

申请号：US15031958

申请日：2014-10-24

Applicant: Becton, Dickinson and Company

Inventor： Mei Yang-Woytowitz ,  Brent Pohl ,  Gary F. Hershner ,  Dwight Livingston ,  Eric Ursprung ,  Gerard Lotz ,  Kevin Bailey ,  Ammon David Lentz ,  Michael A. Brasch ,  Ming-hsiung Yeh ,  Patrick Shawn Beaty ,  Charles C. Yu ,  Timothy M. Wiles ,  Liping Feng ,  Ben Turng ,  Xiaofei Chang ,  Patrick R. Murray

IPC: C12Q1/02 ,  C12M1/00 ,  A61J1/20 ,  C12M1/24 ,  A61J1/05 ,  A61J1/14

Abstract: An apparatus and associated methods of use for a controlled combination of reagents is disclosed. The apparatus includes a vessel 400, a vessel insert 220, and a cap element 200. The vessel 400 has a body portion 410 for receiving a biological sample. The vessel insert 220 receives at least one reagent therein. Preferably, the vessel insert 220 is received in a portion 420 of the vessel 400. The cap element 200 is attached to the vessel 400 to secure the vessel insert 220 in the vessel 400. During use, the vessel insert 220 is adapted to release its contents when the biological sample is introduced into the body portion 410 of the vessel 400 upon application of an intermixing force to the vessel insert 220. A variety of intermixing forces may be applied, depending upon the embodiment of the present invention and its associated methods of use.

Abstract translation: 公开了用于受控组合试剂的装置和相关联的使用方法。 该装置包括容器400，容器插入件220和盖元件200.容器400具有容纳生物样品的主体部分410。 血管插入物220在其中接收至少一种试剂。 优选地，容器插入件220容纳在容器400的部分420中。盖元件200附接到容器400以将容器插入件220固定在容器400中。在使用期间，容器插入件220适于释放其 当向容器插入件220施加混合力时将生物样品引入容器400的主体部分410中的内容物。根据本发明的实施方案及其相关的方法可以应用各种混合力 使用。

公开(公告)号：US20200340029A1

公开(公告)日：2020-10-29

申请号：US16926926

申请日：2020-07-13

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Mei Yang-Woytowitz ,  Brent Pohl ,  Gary F. Hershner ,  Dwight Livingston ,  Eric Ursprung ,  Gerard Lotz ,  Kevin Bailey ,  Ammon David Lentz ,  Michael A. Brasch ,  Ming-hsiung Yeh ,  Patrick Shawn Beaty ,  Charles C. Yu ,  Timothy M. Wiles ,  Liping Feng ,  Ben Turng ,  Xiaofei Chang ,  Patrick R. Murray

IPC: C12Q1/02 ,  A61J1/05 ,  C12M1/24 ,  A61J1/14 ,  A61J1/20 ,  B01L3/00 ,  C12M1/00

Abstract: An apparatus and associated methods of use for a controlled combination of reagents is disclosed. The apparatus includes a vessel 400, a vessel insert 220, and a cap element 200. The vessel 400 has a body portion 410 for receiving a biological sample. The vessel insert 220 receives at least one reagent therein. Preferably, the vessel insert 220 is received in a portion 420 of the vessel 400. The cap element 200 is attached to the vessel 400 to secure the vessel insert 220 in the vessel 400. During use, the vessel insert 220 is adapted to release its contents when the biological sample is introduced into the body portion 410 of the vessel 400 upon application of an intermixing force to the vessel insert 220. A variety of intermixing forces may be applied, depending upon the embodiment of the present invention and its associated methods of use.

公开(公告)号：US10767146B2

公开(公告)日：2020-09-08

申请号：US15031958

申请日：2014-10-24

Applicant: Becton, Dickinson and Company

Inventor： Mei Yang-Woytowitz ,  Brent Pohl ,  Gary F. Hershner ,  Eric Ursprung ,  Michael A. Brasch ,  Ming-hsiung Yeh ,  Patrick Shawn Beaty ,  Charles C. Yu ,  Timothy M. Wiles ,  Liping Feng ,  Ben Turng ,  Xiaofei Chang ,  Patrick R. Murray

IPC: C12Q1/02 ,  C12M1/00 ,  A61J1/20 ,  C12M1/24 ,  A61J1/05 ,  A61J1/14 ,  C12C1/02 ,  B01L3/00

Abstract: An apparatus and associated methods of use for a controlled combination of reagents is disclosed. The apparatus includes a vessel 400, a vessel insert 220, and a cap element 200. The vessel 400 has a body portion 410 for receiving a biological sample. The vessel insert 220 receives at least one reagent therein. Preferably, the vessel insert 220 is received in a portion 420 of the vessel 400. The cap element 200 is attached to the vessel 400 to secure the vessel insert 220 in the vessel 400. During use, the vessel insert 220 is adapted to release its contents when the biological sample is introduced into the body portion 410 of the vessel 400 upon application of an intermixing force to the vessel insert 220. A variety of intermixing forces may be applied, depending upon the embodiment of the present invention and its associated methods of use.

公开(公告)号：US11840719B2

公开(公告)日：2023-12-12

申请号：US16926926

申请日：2020-07-13

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Mei Yang-Woytowitz ,  Brent Pohl ,  Gary F. Hershner ,  Dwight Livingston ,  Eric Ursprung ,  Gerard Lotz ,  Kevin Bailey ,  Ammon David Lentz ,  Michael A. Brasch ,  Ming-hsiung Yeh ,  Patrick Shawn Beaty ,  Charles C. Yu ,  Timothy M. Wiles ,  Liping Feng ,  Ben Turng ,  Xiaofei Chang ,  Patrick R. Murray

IPC: C12Q1/02 ,  A61J1/05 ,  C12M1/24 ,  A61J1/14 ,  A61J1/20 ,  B01L3/00 ,  C12C1/02 ,  C12M1/00

CPC classification number: C12Q1/02 ,  A61J1/05 ,  A61J1/1406 ,  A61J1/1418 ,  A61J1/2006 ,  A61J1/2089 ,  A61J1/2093 ,  B01L3/502 ,  C12C1/02 ,  C12M23/08 ,  C12M23/38 ,  C12M29/00 ,  B01L2200/0621 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/087 ,  B01L2300/0832 ,  G01N2800/26

Abstract: An apparatus and associated methods of use for a controlled combination of reagents is disclosed. The apparatus includes a vessel 400, a vessel insert 220, and a cap element 200. The vessel 400 has a body portion 410 for receiving a biological sample. The vessel insert 220 receives at least one reagent therein. Preferably, the vessel insert 220 is received in a portion 420 of the vessel 400. The cap element 200 is attached to the vessel 400 to secure the vessel insert 220 in the vessel 400. During use, the vessel insert 220 is adapted to release its contents when the biological sample is introduced into the body portion 410 of the vessel 400 upon application of an intermixing force to the vessel insert 220. A variety of intermixing forces may be applied, depending upon the embodiment of the present invention and its associated methods of use.

公开(公告)号：US20220170065A1

公开(公告)日：2022-06-02

申请号：US17521410

申请日：2021-11-08

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Liping Feng ,  William B. Brasso ,  Susan M. Kircher ,  Vanda White ,  Song Shi ,  Xiao Mo ,  Tuan-Linh Ngoc Nguyen ,  Adrien P. Malick ,  Jon E. Salomon ,  John D. Mantlo

IPC: C12Q1/04 ,  G01N33/68 ,  G01N33/483

Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

公开(公告)号：US20130089886A1

公开(公告)日：2013-04-11

申请号：US13647072

申请日：2012-10-08

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Liping Feng ,  William B. Brasso ,  Song Shi ,  Ben Turng ,  Susan M. Kircher ,  Vanda White ,  Dyan Luper ,  Julie Rosales ,  Gretta Campbell ,  Adrien Malick

IPC: C12Q1/24

CPC classification number: C12Q1/24

Abstract: Methods of the invention include the isolation of intact, viable microorganism(s) from positive blood culture (“PBC”) samples for use in downstream analyses such as identification and antimicrobial susceptibility testing (“AST”). The methods involve collecting a portion of the PBC sample, adding a choline-containing solution, lysing the blood cells, isolating the viable microorganism, and performing downstream analysis of the isolated, viable microorganism. The methods can be applied to a variety of gram-positive bacteria, gram-negative bacteria, and/or yeast, and particularly to strains of S. pneumoniae.

Abstract translation: 本发明的方法包括从用于下游分析如鉴定和抗微生物敏感性试验（AST）的阳性血培养（PBC）样品中分离完整的活的微生物。 所述方法包括收集一部分PBC样品，加入含胆碱的溶液，裂解血细胞，分离活微生物，以及进行分离的活的微生物的下游分析。 该方法可以应用于各种革兰氏阳性细菌，革兰氏阴性细菌和/或酵母，特别是肺炎链球菌菌株。

公开(公告)号：US11193158B2

公开(公告)日：2021-12-07

申请号：US16734826

申请日：2020-01-06

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Liping Feng ,  William B. Brasso ,  Susan M. Kircher ,  Vanda White ,  Song Shi ,  Xiao Mo ,  Tuan-Linh Ngoc Nguyen ,  Adrien P. Malick ,  Jon E. Salomon ,  John D. Mantlo

IPC: C12Q1/04 ,  G01N33/68 ,  G01N33/483 ,  H01J49/16 ,  H01J49/40

Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

公开(公告)号：US20200087702A1

公开(公告)日：2020-03-19

申请号：US16677092

申请日：2019-11-07

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Susan M. Kircher ,  Vanda White ,  William B. Brasso ,  Dyan Luper ,  James Y. Zhou ,  Julie L. Rosales ,  Jeffery H. Bruton ,  John D. Mantlo ,  Adrien P. Malick ,  Donald R. Callihan ,  Ben Turng ,  Liping Feng ,  Curtis M. Gosnell ,  Patrick Shawn Beaty ,  John P. Douglass

IPC: C12Q1/14 ,  C12Q1/24 ,  C12N1/02 ,  C12N1/20

Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

公开(公告)号：US20170204448A1

公开(公告)日：2017-07-20

申请号：US15472759

申请日：2017-03-29

Applicant: Becton, Dickinson and Company

Inventor： Liping Feng ,  William B. Brasso ,  Susan M. Kircher ,  Vanda White ,  Song Shi ,  Xiao Mo ,  Tuan-Linh Ngoc Nguyen ,  Adrien P. Malick ,  Jon E. Salomon ,  John D. Mantlo ,  Mary R. Votta ,  Ben Turng ,  Donald R. Callihan ,  Wendy Louise Williams

IPC: C12Q1/04 ,  G01N33/483

CPC classification number: C12Q1/04 ,  G01N33/4833 ,  G01N33/6851 ,  H01J49/164 ,  H01J49/40

Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

公开(公告)号：US11225681B2

公开(公告)日：2022-01-18

申请号：US16677092

申请日：2019-11-07

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Susan M. Kircher ,  Vanda White ,  William B. Brasso ,  Dyan Luper ,  James Y. Zhou ,  Julie L. Rosales ,  Jeffery H. Bruton ,  John D. Mantlo ,  Adrien P. Malick ,  Donald R. Callihan ,  Ben Turng ,  Liping Feng ,  Curtis M. Gosnell ,  Patrick Shawn Beaty ,  John P. Douglass

IPC: C12Q1/14 ,  C12Q1/24 ,  C12N1/20 ,  C12N1/02

Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.