公开(公告)号：US20240318125A1

公开(公告)日：2024-09-26

申请号：US18613621

申请日：2024-03-22

Applicant: DEKA Products Limited Partnership

Inventor： Matthew White ,  Kevin Kim ,  Bryan A. Finseth

IPC: C12M1/36 ,  A61B10/00 ,  C12M1/00 ,  C12M1/24 ,  C12M1/33

CPC classification number: C12M41/48 ,  A61B10/0096 ,  C12M23/08 ,  C12M23/28 ,  C12M27/18 ,  C12M29/00 ,  C12M29/04 ,  C12M29/20 ,  C12M45/02 ,  C12M45/05 ,  C12M45/06

Abstract: A system and method for automatically digesting, culturing and growing a biopsy. The digester is a closed fluid transfer system, including disposable and durable parts, that receives a biopsy, washes the biopsy tissue, digests the tissue to separate the cells from each other, and seeds the cells in a culture flask. In one use, the system receives cells from a patient, expands and multiplies them, and returns them to the patient. A controller coordinates the motion of a plurality of pumps and the status of a plurality of valves, the pumps and the plurality of valves moving the fluid throughout the system. A sensor system assists the controller in volume and flow rate control of the fluids in the system, and in controlling the position of a tube providing air and fluids to a biopsy vial that includes the biopsy.

公开(公告)号：US20240279585A1

公开(公告)日：2024-08-22

申请号：US18651024

申请日：2024-04-30

Applicant: Global Life Sciences Solutions USA LLC

Inventor： Weston Blaine Griffin ,  Alex D. Corwin ,  Xiaohua Zhang ,  Reginald Donovan Smith ,  Zhen Liu ,  Chengkun Zhang ,  Vandana Keskar ,  Brian Michael Davis ,  Kashan Shaikh

IPC: C12M1/00 ,  C12M1/04 ,  C12M1/12 ,  C12M1/24 ,  C12M1/26 ,  C12M1/34 ,  C12N5/0783

CPC classification number: C12M29/04 ,  C12M23/08 ,  C12M23/24 ,  C12M25/02 ,  C12M25/06 ,  C12M29/18 ,  C12M29/26 ,  C12M33/00 ,  C12M41/46 ,  C12N5/0636

Abstract: In an embodiment, a tubing module for a bioprocessing system includes a first tubing holder block configured to receive at least one pump tube and hold the at least one pump tube in position for selective engagement with a peristaltic pump, a second tubing holder block configured to receive a plurality of pinch valve tubes and hold each pinch valve tube of the plurality of pinch valve tubes in position for selective engagement with a respective actuator of a pinch valve array, and wherein the first tubing holder block and the second tubing holder block are interconnected.

公开(公告)号：US20240279581A1

公开(公告)日：2024-08-22

申请号：US18631536

申请日：2024-04-10

Applicant: City of Hope ,  California Institute of Technology

Inventor： Colin Andrew Cook ,  Yuman Fong ,  YU-Chong Tai ,  Saswati Chatterjee

IPC: C12M1/00 ,  C12M1/04 ,  C12M1/12 ,  C12M1/22 ,  C12M1/24 ,  C12N5/071

CPC classification number: C12M23/34 ,  C12M23/08 ,  C12M23/10 ,  C12M23/24 ,  C12M23/38 ,  C12M23/58 ,  C12M25/04 ,  C12M25/06 ,  C12N5/0602

Abstract: A cell cultivation apparatus for cultivating microorganisms and growing cells at high density is provided. The apparatus includes a membrane comprising multiple surface features on a first side of the membrane for cell placement. The surface features comprising one or more compartments within which a cell can be located. The membrane includes a material that is at least partially permeable to gas. A second side of the membrane defines a gas region. The second side of the membrane is separated from the first side of the membrane by the membrane. The apparatus further includes a media region for receiving media. The compartments are configured to at least partially reduce media flow shear forces on one or more cells in the compartments. The surface features may be ridges, protrusions, fins, wells, and/or posts.

公开(公告)号：US11987781B2

公开(公告)日：2024-05-21

申请号：US17360585

申请日：2021-06-28

Applicant: LG Chem, Ltd.

Inventor： Eun Byeol Ko ,  Jung Hye Won ,  Nak Hee Choi ,  Ji Seok Lee ,  Eun Yeong Jin ,  Hae Sung Yun

IPC: C12M1/107 ,  C12M1/00 ,  C12M1/04 ,  C12M1/24 ,  C12M1/34

CPC classification number: C12M23/36 ,  C12M23/08 ,  C12M23/38 ,  C12M29/00 ,  C12M41/14 ,  C12M41/34 ,  C12M41/46

Abstract: The present invention relates to a gas collection device and is directed to a gas collection device for collecting a gas which is generated while microorganisms are cultured in a super absorbent polymer product. The gas collection device may comprise a constant temperature chamber having an interior that is configured to be maintained at a set temperature; a culture flask unit located inside the constant temperature chamber and configured to culture a bacteria therein; an adsorption unit located outside the constant temperature chamber and configured to receive a gas inside the culture flask unit; a pump unit connected to a rear end of the adsorption unit and configured to suck the gas inside the culture flask unit into the adsorption unit; and a mass flow controller located outside the constant temperature chamber and configured to control a flow rate of the gas sucked into the adsorption unit.

公开(公告)号：US11970682B2

公开(公告)日：2024-04-30

申请号：US17735551

申请日：2022-05-03

Applicant: CORNING INCORPORATED

Inventor： Vasiliy Nikolaevich Goral

IPC: C12M3/06 ,  C12M1/24 ,  C12M1/32 ,  C12M3/00

CPC classification number: C12M23/16 ,  C12M23/08 ,  C12M23/12 ,  C12M23/44

Abstract: A cell culture vessel includes a base defining a base plane extending in a first direction and a second direction perpendicular to the first direction, a plurality of cell culture chambers stacked one atop another, each cell culture chamber having a top, a bottom and sidewalls, each of the top, bottom and sidewalls having an interior surface, wherein at least the bottom surface has an array of microcavities supporting the culture of cells as spheroids and each bottom surface is at an angle with respect to the plane of a table or surface upon which the vessel sits. Further, liquid can flow into each cell culture chamber via an inlet and out of each cell culture chamber via an outlet. The angled cell culture surface allows the cell culture chambers to be perfused or allows media changes without dislodging spheroids from microcavities.

公开(公告)号：US11905506B2

公开(公告)日：2024-02-20

申请号：US17591747

申请日：2022-02-03

Applicant: CORNING INCORPORATED

Inventor： Gregory Roger Martin ,  Allison Jean Tanner

IPC: C12M3/00 ,  C12M1/00 ,  C12M1/24 ,  C12M1/12 ,  C12M1/04 ,  C12N5/071 ,  C12M1/34

CPC classification number: C12M23/08 ,  C12M23/04 ,  C12M23/24 ,  C12M23/34 ,  C12M23/38 ,  C12M23/58 ,  C12M25/02 ,  C12M25/04 ,  C12M25/06 ,  C12M29/04 ,  C12M41/00 ,  C12N5/0602

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

公开(公告)号：US11884912B2

公开(公告)日：2024-01-30

申请号：US16727057

申请日：2019-12-26

Applicant: Industrial Technology Research Institute

Inventor： Kuo-Hsing Wen ,  Ting-Hsuan Chen ,  Cheng-Tai Chen ,  Chien-An Chen ,  Su-Fung Chiu ,  Yung-Chi Chang ,  Nien-Jen Chou ,  Ping-Jung Wu ,  Shaw-Hwa Parng ,  Pei-Shin Jiang

IPC: C12M1/34 ,  C12M1/00 ,  C12M1/02 ,  C12M1/24

CPC classification number: C12M41/46 ,  C12M23/08 ,  C12M27/00 ,  C12M29/00 ,  C12M41/26

Abstract: A cell culture device includes a culture unit, a gas supply unit, a first pressure unit, at least one inspecting unit and a control unit. The culture unit contains a cell culture liquid. The gas supply unit, connected with the culture unit, is used for transmitting a culture gas into the culture unit. The first pressure unit, connected with the culture unit, is used for applying a pressure to the cell culture liquid in the culture unit. The at least one inspecting unit, connected with the culture unit, is used for receiving the cell culture liquid for inspection. The control unit, electrically coupled with the culture unit, the first pressure unit, the gas supply unit and the at least one inspecting unit, is used for monitoring corresponding condition parameters to determine respective operations. In addition, a cell culture method for the cell culture device is also provided.

公开(公告)号：US20230357686A1

公开(公告)日：2023-11-09

申请号：US18219266

申请日：2023-07-07

Applicant: CORNING INCORPORATED

Inventor： William Joseph Lacey ,  Gregory Roger Martin ,  Allison Jean Tanner

IPC: C12M1/24 ,  C12M1/00 ,  C12M1/12

CPC classification number: C12M23/08 ,  C12M23/34 ,  C12M25/00

Abstract: A cell culture vessel includes a vessel body, support columns, and a stabilizer device. The vessel body defines a cell culture chamber enclosed between a bottom wall and a top wall. The support column is within the cell culture chamber and extends between the top wall and the bottom wall. The stabilizer device covers a width and length of the cell culture chamber and has a column engaging structure that is sized to slidingly engage the support column such that the stabilizer device is movable along the support column as a liquid culture medium is received in the cell culture chamber. The support column guides the stabilizer device along a length of the support column as the stabilizer device rises with rising liquid level in the cell culture chamber during a liquid culture medium filling operation.

公开(公告)号：US20230303967A1

公开(公告)日：2023-09-28

申请号：US18202781

申请日：2023-05-26

Applicant: Wilson Wolf Manufacturing

Inventor： John R. Wilson

IPC: C12N5/071 ,  C12M1/04 ,  C12M1/00 ,  C12M1/24

CPC classification number: C12N5/0602 ,  C12M23/24 ,  C12M23/34 ,  C12M23/08

Abstract: This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.

公开(公告)号：US11732227B2

公开(公告)日：2023-08-22

申请号：US16629656

申请日：2018-07-13

Applicant: CORNING INCORPORATED

Inventor： William Joseph Lacey ,  Gregory Roger Martin ,  Allison Jean Tanner

IPC: C12M1/24 ,  C12M1/00 ,  C12M1/12

CPC classification number: C12M23/08 ,  C12M23/34 ,  C12M25/00

Abstract: A cell culture vessel includes a vessel body, support columns, and a stabilizer device. The vessel body defines a cell culture chamber enclosed between a bottom wall and a top wall. The support column is within the cell culture chamber and extends between the top wall and the bottom wall. The stabilizer device covers a width and length of the cell culture chamber and has a column engaging structure that is sized to slidingly engage the support column such that the stabilizer device is movable along the support column as a liquid culture medium is received in the cell culture chamber. The support column guides the stabilizer device along a length of the support column as the stabilizer device rises with rising liquid level in the cell culture chamber during a liquid culture medium filling operation.