公开(公告)号：US20200087702A1

公开(公告)日：2020-03-19

申请号：US16677092

申请日：2019-11-07

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Susan M. Kircher ,  Vanda White ,  William B. Brasso ,  Dyan Luper ,  James Y. Zhou ,  Julie L. Rosales ,  Jeffery H. Bruton ,  John D. Mantlo ,  Adrien P. Malick ,  Donald R. Callihan ,  Ben Turng ,  Liping Feng ,  Curtis M. Gosnell ,  Patrick Shawn Beaty ,  John P. Douglass

IPC: C12Q1/14 ,  C12Q1/24 ,  C12N1/02 ,  C12N1/20

Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

公开(公告)号：US11225681B2

公开(公告)日：2022-01-18

申请号：US16677092

申请日：2019-11-07

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Susan M. Kircher ,  Vanda White ,  William B. Brasso ,  Dyan Luper ,  James Y. Zhou ,  Julie L. Rosales ,  Jeffery H. Bruton ,  John D. Mantlo ,  Adrien P. Malick ,  Donald R. Callihan ,  Ben Turng ,  Liping Feng ,  Curtis M. Gosnell ,  Patrick Shawn Beaty ,  John P. Douglass

IPC: C12Q1/14 ,  C12Q1/24 ,  C12N1/20 ,  C12N1/02

Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

公开(公告)号：US10519482B2

公开(公告)日：2019-12-31

申请号：US14381388

申请日：2013-02-28

Applicant: Becton, Dickinson and Company

Inventor： Susan M. Kircher ,  Vanda White ,  William B. Brasso ,  Dyan Luper ,  James Y. Zhou ,  Julie L. Rosales ,  Jeffery H. Bruton ,  John D. Mantlo ,  Adrien P. Malick ,  Donald R. Callihan ,  Ben Turng ,  Liping Feng ,  Curtis M. Gosnell ,  Patrick Shawn Beaty ,  John P. Douglass

IPC: C12Q1/14 ,  C12Q1/24 ,  C12N1/02 ,  C12N1/20 ,  C12N1/04 ,  C12Q1/06

Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

公开(公告)号：US20150125895A1

公开(公告)日：2015-05-07

申请号：US14381388

申请日：2013-02-28

Applicant: Becton, Dickinson and Company

Inventor： Susan M. Kircher ,  Vanda White ,  William B. Brasso ,  Dyan Luper ,  James Y. Zhou ,  Julie L. Rosales ,  Jeffery H. Bruton ,  John D. Mantlo ,  Adrien P. Malick ,  Donald R. Callihan ,  Ben Turng ,  Liping Feng ,  Curtis M. Gosnell ,  Patrick Shawn Beaty ,  John P. Douglass

IPC: C12Q1/14 ,  C12N1/20 ,  C12Q1/24

CPC classification number: C12Q1/14 ,  C12N1/02 ,  C12N1/20 ,  C12Q1/24

Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

Abstract translation: 本文公开的各种实施方案提供了用于从阳性血培养样品中快速分离存活的微生物细胞（包括肺炎链球菌）的试剂和方法。 所得到的微生物颗粒可用于鉴定和基于生长的方法如抗微生物敏感性测试。 本文所述的缓冲剂可以含有碱溶液，非离子洗涤剂，硫醇和任选的氯化铵。 所公开的方法提供了一种用于仅从一个样品制备管和离心同时从PBC样品中快速分离和浓缩活的微生物的方法，同时从哺乳动物血细胞中除去可能干扰鉴定方法的细胞碎片。