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公开(公告)号：US20220170065A1
公开(公告)日：2022-06-02
申请号：US17521410
申请日：2021-11-08
Applicant: BECTON DICKINSON AND COMPANY
Inventor： Liping Feng , William B. Brasso , Susan M. Kircher , Vanda White , Song Shi , Xiao Mo , Tuan-Linh Ngoc Nguyen , Adrien P. Malick , Jon E. Salomon , John D. Mantlo
IPC: C12Q1/04 , G01N33/68 , G01N33/483
Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.
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公开(公告)号：US09995745B2
公开(公告)日：2018-06-12
申请号：US14932693
申请日：2015-11-04
Applicant: ARBOR VITA CORPORATION , Becton, Dickinson and Company
Inventor： Adrien P. Malick , Virginia M. Crews , Julie L. Rosales , Carrie S. Ferguson , Jeff H. Bruton , Robert J. Beadenkopf , John Mantlo
IPC: G01N33/543 , C12N15/52 , C07K1/14 , G01N33/569 , G01N1/40
CPC classification number: G01N33/54393 , C07K1/145 , C12N15/52 , C12N2710/20022 , G01N1/4044 , G01N33/56983 , G01N2333/025 , G01N2333/16 , G01N2469/10
Abstract: Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.
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公开(公告)号：US11193158B2
公开(公告)日：2021-12-07
申请号：US16734826
申请日：2020-01-06
Applicant: BECTON DICKINSON AND COMPANY
Inventor： Liping Feng , William B. Brasso , Susan M. Kircher , Vanda White , Song Shi , Xiao Mo , Tuan-Linh Ngoc Nguyen , Adrien P. Malick , Jon E. Salomon , John D. Mantlo
IPC: C12Q1/04 , G01N33/68 , G01N33/483 , H01J49/16 , H01J49/40
Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.
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4.发明申请公开(公告)号：US20200087702A1
公开(公告)日：2020-03-19
申请号：US16677092
申请日：2019-11-07
Applicant: BECTON DICKINSON AND COMPANY
Inventor： Susan M. Kircher , Vanda White , William B. Brasso , Dyan Luper , James Y. Zhou , Julie L. Rosales , Jeffery H. Bruton , John D. Mantlo , Adrien P. Malick , Donald R. Callihan , Ben Turng , Liping Feng , Curtis M. Gosnell , Patrick Shawn Beaty , John P. Douglass
Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.
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公开(公告)号：US20170204448A1
公开(公告)日：2017-07-20
申请号：US15472759
申请日：2017-03-29
Applicant: Becton, Dickinson and Company
Inventor： Liping Feng , William B. Brasso , Susan M. Kircher , Vanda White , Song Shi , Xiao Mo , Tuan-Linh Ngoc Nguyen , Adrien P. Malick , Jon E. Salomon , John D. Mantlo , Mary R. Votta , Ben Turng , Donald R. Callihan , Wendy Louise Williams
IPC: C12Q1/04 , G01N33/483
CPC classification number: C12Q1/04 , G01N33/4833 , G01N33/6851 , H01J49/164 , H01J49/40
Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.
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Patent Agency Ranking
6.发明授权公开(公告)号：US11225681B2
公开(公告)日：2022-01-18
申请号：US16677092
申请日：2019-11-07
Applicant: BECTON DICKINSON AND COMPANY
Inventor： Susan M. Kircher , Vanda White , William B. Brasso , Dyan Luper , James Y. Zhou , Julie L. Rosales , Jeffery H. Bruton , John D. Mantlo , Adrien P. Malick , Donald R. Callihan , Ben Turng , Liping Feng , Curtis M. Gosnell , Patrick Shawn Beaty , John P. Douglass
Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.
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公开(公告)号：US20200149085A1
公开(公告)日：2020-05-14
申请号：US16734826
申请日：2020-01-06
Applicant: BECTON DICKINSON AND COMPANY
Inventor： Liping Feng , William B. Brasso , Susan M. Kircher , Vanda White , Song Shi , Xiao Mo , Tuan-Linh Ngoc Nguyen , Adrien P. Malick , Jon E. Salomon , John D. Mantlo
IPC: C12Q1/04 , G01N33/68 , G01N33/483
Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.
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公开(公告)号：US10557162B2
公开(公告)日：2020-02-11
申请号：US15472759
申请日：2017-03-29
Applicant: Becton, Dickinson and Company
Inventor： Liping Feng , William B. Brasso , Susan M. Kircher , Vanda White , Song Shi , Xiao Mo , Tuan-Linh Ngoc Nguyen , Adrien P. Malick , Jon E. Salomon , John D. Mantlo
IPC: C12Q1/04 , G01N33/68 , G01N33/483 , H01J49/16 , H01J49/40
Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.
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9.发明授权公开(公告)号：US10519482B2
公开(公告)日：2019-12-31
申请号：US14381388
申请日：2013-02-28
Applicant: Becton, Dickinson and Company
Inventor： Susan M. Kircher , Vanda White , William B. Brasso , Dyan Luper , James Y. Zhou , Julie L. Rosales , Jeffery H. Bruton , John D. Mantlo , Adrien P. Malick , Donald R. Callihan , Ben Turng , Liping Feng , Curtis M. Gosnell , Patrick Shawn Beaty , John P. Douglass
Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.
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公开(公告)号：US20160216258A1
公开(公告)日：2016-07-28
申请号：US14932693
申请日：2015-11-04
Applicant: ARBOR VITA CORPORATION , Becton, Dickinson and Company
Inventor： Adrien P. Malick , Virginia M. Crews , Julie L. Rosales , Carrie S. Ferguson , Jeff H. Bruton , Robert J. Beadenkopf , John Mantlo
IPC: G01N33/543 , G01N33/569 , G01N1/40
CPC classification number: G01N33/54393 , C07K1/145 , C12N15/52 , C12N2710/20022 , G01N1/4044 , G01N33/56983 , G01N2333/025 , G01N2333/16 , G01N2469/10
Abstract: Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.
Abstract translation: 提供了从细胞如含有病毒蛋白的细胞或细胞样品中产生蛋白质提取物的方法。 一般来说，所述方法可以包括：将细胞的pH增加到至少约pH 10.0的pH以产生中间体组合物，然后在非离子洗涤剂如聚氧乙烯烷基醚的存在下中和 中间体组合物的pH以产生蛋白质提取物。 这些方法可以结合用于检测样品中的一种或多种靶蛋白的方法，例如病毒蛋白。 还提供了用于实践主题方法的系统，试剂盒和组合物。
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