公开(公告)号：US20220170065A1

公开(公告)日：2022-06-02

申请号：US17521410

申请日：2021-11-08

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Liping Feng ,  William B. Brasso ,  Susan M. Kircher ,  Vanda White ,  Song Shi ,  Xiao Mo ,  Tuan-Linh Ngoc Nguyen ,  Adrien P. Malick ,  Jon E. Salomon ,  John D. Mantlo

IPC: C12Q1/04 ,  G01N33/68 ,  G01N33/483

Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

公开(公告)号：US11193158B2

公开(公告)日：2021-12-07

申请号：US16734826

申请日：2020-01-06

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Liping Feng ,  William B. Brasso ,  Susan M. Kircher ,  Vanda White ,  Song Shi ,  Xiao Mo ,  Tuan-Linh Ngoc Nguyen ,  Adrien P. Malick ,  Jon E. Salomon ,  John D. Mantlo

IPC: C12Q1/04 ,  G01N33/68 ,  G01N33/483 ,  H01J49/16 ,  H01J49/40

Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

公开(公告)号：US20170204448A1

公开(公告)日：2017-07-20

申请号：US15472759

申请日：2017-03-29

Applicant: Becton, Dickinson and Company

Inventor： Liping Feng ,  William B. Brasso ,  Susan M. Kircher ,  Vanda White ,  Song Shi ,  Xiao Mo ,  Tuan-Linh Ngoc Nguyen ,  Adrien P. Malick ,  Jon E. Salomon ,  John D. Mantlo ,  Mary R. Votta ,  Ben Turng ,  Donald R. Callihan ,  Wendy Louise Williams

IPC: C12Q1/04 ,  G01N33/483

CPC classification number: C12Q1/04 ,  G01N33/4833 ,  G01N33/6851 ,  H01J49/164 ,  H01J49/40

Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

公开(公告)号：US20200149085A1

公开(公告)日：2020-05-14

申请号：US16734826

申请日：2020-01-06

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Liping Feng ,  William B. Brasso ,  Susan M. Kircher ,  Vanda White ,  Song Shi ,  Xiao Mo ,  Tuan-Linh Ngoc Nguyen ,  Adrien P. Malick ,  Jon E. Salomon ,  John D. Mantlo

IPC: C12Q1/04 ,  G01N33/68 ,  G01N33/483

Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

公开(公告)号：US10557162B2

公开(公告)日：2020-02-11

申请号：US15472759

申请日：2017-03-29

Applicant: Becton, Dickinson and Company

Inventor： Liping Feng ,  William B. Brasso ,  Susan M. Kircher ,  Vanda White ,  Song Shi ,  Xiao Mo ,  Tuan-Linh Ngoc Nguyen ,  Adrien P. Malick ,  Jon E. Salomon ,  John D. Mantlo

IPC: C12Q1/04 ,  G01N33/68 ,  G01N33/483 ,  H01J49/16 ,  H01J49/40

Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.