公开(公告)号：US11840719B2

公开(公告)日：2023-12-12

申请号：US16926926

申请日：2020-07-13

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Mei Yang-Woytowitz ,  Brent Pohl ,  Gary F. Hershner ,  Dwight Livingston ,  Eric Ursprung ,  Gerard Lotz ,  Kevin Bailey ,  Ammon David Lentz ,  Michael A. Brasch ,  Ming-hsiung Yeh ,  Patrick Shawn Beaty ,  Charles C. Yu ,  Timothy M. Wiles ,  Liping Feng ,  Ben Turng ,  Xiaofei Chang ,  Patrick R. Murray

IPC: C12Q1/02 ,  A61J1/05 ,  C12M1/24 ,  A61J1/14 ,  A61J1/20 ,  B01L3/00 ,  C12C1/02 ,  C12M1/00

CPC classification number: C12Q1/02 ,  A61J1/05 ,  A61J1/1406 ,  A61J1/1418 ,  A61J1/2006 ,  A61J1/2089 ,  A61J1/2093 ,  B01L3/502 ,  C12C1/02 ,  C12M23/08 ,  C12M23/38 ,  C12M29/00 ,  B01L2200/0621 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/087 ,  B01L2300/0832 ,  G01N2800/26

Abstract: An apparatus and associated methods of use for a controlled combination of reagents is disclosed. The apparatus includes a vessel 400, a vessel insert 220, and a cap element 200. The vessel 400 has a body portion 410 for receiving a biological sample. The vessel insert 220 receives at least one reagent therein. Preferably, the vessel insert 220 is received in a portion 420 of the vessel 400. The cap element 200 is attached to the vessel 400 to secure the vessel insert 220 in the vessel 400. During use, the vessel insert 220 is adapted to release its contents when the biological sample is introduced into the body portion 410 of the vessel 400 upon application of an intermixing force to the vessel insert 220. A variety of intermixing forces may be applied, depending upon the embodiment of the present invention and its associated methods of use.

公开(公告)号：US20160265022A1

公开(公告)日：2016-09-15

申请号：US15031958

申请日：2014-10-24

Applicant: Becton, Dickinson and Company

Inventor： Mei Yang-Woytowitz ,  Brent Pohl ,  Gary F. Hershner ,  Dwight Livingston ,  Eric Ursprung ,  Gerard Lotz ,  Kevin Bailey ,  Ammon David Lentz ,  Michael A. Brasch ,  Ming-hsiung Yeh ,  Patrick Shawn Beaty ,  Charles C. Yu ,  Timothy M. Wiles ,  Liping Feng ,  Ben Turng ,  Xiaofei Chang ,  Patrick R. Murray

IPC: C12Q1/02 ,  C12M1/00 ,  A61J1/20 ,  C12M1/24 ,  A61J1/05 ,  A61J1/14

Abstract: An apparatus and associated methods of use for a controlled combination of reagents is disclosed. The apparatus includes a vessel 400, a vessel insert 220, and a cap element 200. The vessel 400 has a body portion 410 for receiving a biological sample. The vessel insert 220 receives at least one reagent therein. Preferably, the vessel insert 220 is received in a portion 420 of the vessel 400. The cap element 200 is attached to the vessel 400 to secure the vessel insert 220 in the vessel 400. During use, the vessel insert 220 is adapted to release its contents when the biological sample is introduced into the body portion 410 of the vessel 400 upon application of an intermixing force to the vessel insert 220. A variety of intermixing forces may be applied, depending upon the embodiment of the present invention and its associated methods of use.

Abstract translation: 公开了用于受控组合试剂的装置和相关联的使用方法。 该装置包括容器400，容器插入件220和盖元件200.容器400具有容纳生物样品的主体部分410。 血管插入物220在其中接收至少一种试剂。 优选地，容器插入件220容纳在容器400的部分420中。盖元件200附接到容器400以将容器插入件220固定在容器400中。在使用期间，容器插入件220适于释放其 当向容器插入件220施加混合力时将生物样品引入容器400的主体部分410中的内容物。根据本发明的实施方案及其相关的方法可以应用各种混合力 使用。

公开(公告)号：US20130172309A1

公开(公告)日：2013-07-04

申请号：US13692189

申请日：2012-12-03

Applicant: Becton, Dickinson and Company

Inventor： Craig C. Whiteford ,  Charles C. Yu

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/68 ,  C12Q2600/16 ,  Y10S435/975

Abstract: Compositions and methods for the rapid and sensitive detection of a carbapenemase in a sample are provided. The compositions include novel primer and probe compositions for use in detecting the presence of this enzyme in a sample, particularly using PCR methods. These primers and probe sets can be used in amplification methods (such as PCR, particularly quantitative PCR) and packaged into kits for use in amplification methods for the purpose of detecting carbapenemase in a test sample, particularly a patient sample, particularly a direct sample. Thus, in one embodiment, the present invention provides for novel oligonucleotide primers set forth in SEQ ID NOs:1, 2, 4, 5, 7, 8, 14, 15, 17, 18, and 20, and the novel oligonucleotide probe sequences set forth in SEQ ID NOs:3, 6, 9, 16, and 19. These sequences can be used in a method of detecting carbapenemase in a sample.

Abstract translation: 提供了用于快速和敏感地检测样品中碳青霉烯酶的组合物和方法。 组合物包括用于检测样品中该酶的存在的新型引物和探针组合物，特别是使用PCR方法。 这些引物和探针组可用于扩增方法（如PCR，特别是定量PCR）中，并包装成用于扩增方法的试剂盒，用于检测测试样品中的碳青霉烯酶，特别是患者样品，特别是直接样品。 因此，在一个实施方案中，本发明提供了SEQ ID NO：1,2,4,5,7,8,14,15,17,18和20所示的新的寡核苷酸引物，并且新的寡核苷酸探针序列 如SEQ ID NO：3,6,9,16和19所示。这些序列可用于检测样品中碳青霉烯酶的方法。

公开(公告)号：US20200340029A1

公开(公告)日：2020-10-29

申请号：US16926926

申请日：2020-07-13

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Mei Yang-Woytowitz ,  Brent Pohl ,  Gary F. Hershner ,  Dwight Livingston ,  Eric Ursprung ,  Gerard Lotz ,  Kevin Bailey ,  Ammon David Lentz ,  Michael A. Brasch ,  Ming-hsiung Yeh ,  Patrick Shawn Beaty ,  Charles C. Yu ,  Timothy M. Wiles ,  Liping Feng ,  Ben Turng ,  Xiaofei Chang ,  Patrick R. Murray

IPC: C12Q1/02 ,  A61J1/05 ,  C12M1/24 ,  A61J1/14 ,  A61J1/20 ,  B01L3/00 ,  C12M1/00

Abstract: An apparatus and associated methods of use for a controlled combination of reagents is disclosed. The apparatus includes a vessel 400, a vessel insert 220, and a cap element 200. The vessel 400 has a body portion 410 for receiving a biological sample. The vessel insert 220 receives at least one reagent therein. Preferably, the vessel insert 220 is received in a portion 420 of the vessel 400. The cap element 200 is attached to the vessel 400 to secure the vessel insert 220 in the vessel 400. During use, the vessel insert 220 is adapted to release its contents when the biological sample is introduced into the body portion 410 of the vessel 400 upon application of an intermixing force to the vessel insert 220. A variety of intermixing forces may be applied, depending upon the embodiment of the present invention and its associated methods of use.

公开(公告)号：US10767146B2

公开(公告)日：2020-09-08

申请号：US15031958

申请日：2014-10-24

Applicant: Becton, Dickinson and Company

Inventor： Mei Yang-Woytowitz ,  Brent Pohl ,  Gary F. Hershner ,  Eric Ursprung ,  Michael A. Brasch ,  Ming-hsiung Yeh ,  Patrick Shawn Beaty ,  Charles C. Yu ,  Timothy M. Wiles ,  Liping Feng ,  Ben Turng ,  Xiaofei Chang ,  Patrick R. Murray

IPC: C12Q1/02 ,  C12M1/00 ,  A61J1/20 ,  C12M1/24 ,  A61J1/05 ,  A61J1/14 ,  C12C1/02 ,  B01L3/00

Abstract: An apparatus and associated methods of use for a controlled combination of reagents is disclosed. The apparatus includes a vessel 400, a vessel insert 220, and a cap element 200. The vessel 400 has a body portion 410 for receiving a biological sample. The vessel insert 220 receives at least one reagent therein. Preferably, the vessel insert 220 is received in a portion 420 of the vessel 400. The cap element 200 is attached to the vessel 400 to secure the vessel insert 220 in the vessel 400. During use, the vessel insert 220 is adapted to release its contents when the biological sample is introduced into the body portion 410 of the vessel 400 upon application of an intermixing force to the vessel insert 220. A variety of intermixing forces may be applied, depending upon the embodiment of the present invention and its associated methods of use.

公开(公告)号：US09085806B2

公开(公告)日：2015-07-21

申请号：US13692189

申请日：2012-12-03

Applicant: Becton, Dickinson and Company

Inventor： Craig C. Whiteford ,  Charles C. Yu

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/68 ,  C12Q2600/16 ,  Y10S435/975

Abstract: Compositions and methods for the rapid and sensitive detection of a carbapenemase in a sample are provided. The compositions include novel primer and probe compositions for use in detecting the presence of this enzyme in a sample, particularly using PCR methods. These primers and probe sets can be used in amplification methods (such as PCR, particularly quantitative PCR) and packaged into kits for use in amplification methods for the purpose of detecting carbapenemase in a test sample, particularly a patient sample, particularly a direct sample. Thus, in one embodiment, the present invention provides for novel oligonucleotide primers set forth in SEQ ID NOs:1, 2, 4, 5, 7, 8, 14, 15, 17, 18, and 20, and the novel oligonucleotide probe sequences set forth in SEQ ID NOs:3, 6, 9, 16, and 19. These sequences can be used in a method of detecting carbapenemase in a sample.

Abstract translation: 提供了用于快速和敏感地检测样品中碳青霉烯酶的组合物和方法。 组合物包括用于检测样品中该酶的存在的新型引物和探针组合物，特别是使用PCR方法。 这些引物和探针组可用于扩增方法（如PCR，特别是定量PCR）中，并包装成用于扩增方法的试剂盒，用于检测测试样品中的碳青霉烯酶，特别是患者样品，特别是直接样品。 因此，在一个实施方案中，本发明提供了SEQ ID NO：1,2,4,5,7,8,14,15,17,18和20所示的新的寡核苷酸引物，并且新的寡核苷酸探针序列 如SEQ ID NO：3,6,9,16和19所示。这些序列可用于检测样品中碳青霉烯酶的方法。