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公开(公告)号：US11845919B2
公开(公告)日：2023-12-19
申请号：US15573266
申请日：2016-05-13
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Mei Yang-Woytowitz , Song Shi , Xiaofei Chang , James Y. Zhou , Michael A. Brasch
CPC classification number: C12M23/08 , C12M23/00 , C12M41/46 , C12N5/0634 , C12N2501/999
Abstract: A container for culturing a blood sample. The container has a reservoir that is no larger than about 40 ml in volume with culture media therein varying in amount by volume 0.5 ml to about 20 ml. The container is adapted to receive a blood sample drawn from a patient, wherein the blood volume is about 1 ml to about 20 ml. In some embodiments the ratio of blood volume to culture media volume is about 2:1 to about 1:2 and the volume of blood does not exceed about 10 ml. In some embodiments, the media is lytic media. A method for using the container to culture a blood sample is also contemplated. In such method, the container is inoculated with the blood sample. In certain embodiments, the volume of the blood sample does not exceed 10 mls.
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公开(公告)号：US20220170065A1
公开(公告)日：2022-06-02
申请号：US17521410
申请日：2021-11-08
Applicant: BECTON DICKINSON AND COMPANY
Inventor： Liping Feng , William B. Brasso , Susan M. Kircher , Vanda White , Song Shi , Xiao Mo , Tuan-Linh Ngoc Nguyen , Adrien P. Malick , Jon E. Salomon , John D. Mantlo
IPC: C12Q1/04 , G01N33/68 , G01N33/483
Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.
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公开(公告)号：US20190078043A1
公开(公告)日：2019-03-14
申请号：US15573266
申请日：2016-05-13
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Mei Yang-Woytowitz , Song Shi , Xiaofei Chang , James Y. Zhou , Michael A. Brasch
Abstract: A container for culturing a blood sample. The container has a reservoir that is no larger than about 40 ml in volume with culture media therein varying in amount by volume 0.5 ml to about 20 ml. The container is adapted to receive a blood sample drawn from a patient, wherein the blood volume is about 1 ml to about 20 ml. In some embodiments the ratio of blood volume to culture media volume is about 2:1 to about 1:2 and the volume of blood does not exceed about 10 ml. In some embodiments, the media is lytic media. A method for using the container to culture a blood sample is also contemplated. In such method, the container is inoculated with the blood sample. In certain embodiments, the volume of the blood sample does not exceed 10 mls.
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公开(公告)号：US10221453B2
公开(公告)日：2019-03-05
申请号：US15889035
申请日：2018-02-05
Applicant: Becton, Dickinson and Company
Inventor： Song Shi , Richard L. Moore , James Garrett
IPC: G01N33/74 , G01N33/92 , C12Q1/6881
Abstract: The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.
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公开(公告)号：US09933341B2
公开(公告)日：2018-04-03
申请号：US14390596
申请日：2013-04-04
Applicant: Becton, Dickinson and Company
Inventor： Xiao Li , Yongqiang Zhang , Kenneth Anthony Kopher , John D. Mantlo , William Alfred Pope , Song Shi , Axel A. Yup
CPC classification number: G01N1/30 , C12Q1/04 , C12Q1/06 , G01N2001/302
Abstract: Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.
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Patent Agency Ranking
6.发明申请METHOD FOR DIRECT AND RAPID IDENTIFICATION OF MICROORGANISMS AND ANTIMICROBIAL SUSCEPTIBILITY TESTING FROM POSITIVE BLOOD CULTURES 有权用于直接和快速鉴定微生物的方法和来自阳性血液文化的抗微生物可检测性公开(公告)号：US20130089886A1
公开(公告)日：2013-04-11
申请号：US13647072
申请日：2012-10-08
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Liping Feng , William B. Brasso , Song Shi , Ben Turng , Susan M. Kircher , Vanda White , Dyan Luper , Julie Rosales , Gretta Campbell , Adrien Malick
IPC: C12Q1/24
CPC classification number: C12Q1/24
Abstract: Methods of the invention include the isolation of intact, viable microorganism(s) from positive blood culture (“PBC”) samples for use in downstream analyses such as identification and antimicrobial susceptibility testing (“AST”). The methods involve collecting a portion of the PBC sample, adding a choline-containing solution, lysing the blood cells, isolating the viable microorganism, and performing downstream analysis of the isolated, viable microorganism. The methods can be applied to a variety of gram-positive bacteria, gram-negative bacteria, and/or yeast, and particularly to strains of S. pneumoniae.
Abstract translation: 本发明的方法包括从用于下游分析如鉴定和抗微生物敏感性试验（AST）的阳性血培养（PBC）样品中分离完整的活的微生物。 所述方法包括收集一部分PBC样品，加入含胆碱的溶液，裂解血细胞，分离活微生物，以及进行分离的活的微生物的下游分析。 该方法可以应用于各种革兰氏阳性细菌，革兰氏阴性细菌和/或酵母，特别是肺炎链球菌菌株。
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公开(公告)号：US20180002752A1
公开(公告)日：2018-01-04
申请号：US15649098
申请日：2017-07-13
Applicant: Becton, Dickinson and Company
Inventor： Song Shi , Richard L. Moore , James Garrett
CPC classification number: C12Q1/6881 , C12Q2600/158 , G01N33/74 , G01N33/92 , G01N2333/90209 , G01N2333/91051 , G01N2800/26
Abstract: The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.
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公开(公告)号：US09708661B2
公开(公告)日：2017-07-18
申请号：US14743735
申请日：2015-06-18
Applicant: Becton, Dickinson and Company
Inventor： Song Shi , Richard L. Moore , James Garrett
CPC classification number: C12Q1/6881 , C12Q2600/158 , G01N33/74 , G01N33/92 , G01N2333/90209 , G01N2333/91051 , G01N2800/26
Abstract: The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.
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公开(公告)号：US20160011087A1
公开(公告)日：2016-01-14
申请号：US14774392
申请日：2013-04-04
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Xiao Li , Kenneth Anthony Kopher , John D. Mantlo , William Alfred Pope , Song Shi , Axel A. Yup
CPC classification number: G01N1/30 , C12Q1/04 , C12Q1/06 , G01N2001/302
Abstract: A process for determining one of the presence, absence, or total of microorganisms (e.g. bacteria) in a sample. According to the process, a biological sample containing complex matrices is obtained. The sample is first combined with a resin to adsorb complex matrices from the sample. The resin is removed from the biological sample. The sample so prepared is then analyzed by flow cytometry.
Abstract translation: 确定样品中微生物（例如细菌）的存在，不存在或总计之一的方法。 根据该方法，得到含有复合基质的生物样品。 样品首先与树脂组合以从样品中吸附复杂的基质。 从生物样品中除去树脂。 然后通过流式细胞术分析如此制备的样品。
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公开(公告)号：US20150231626A1
公开(公告)日：2015-08-20
申请号：US14426555
申请日：2013-09-04
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Song Shi , Mei Yang , Klaus W. Berndt , Robert J. Losada , Jamieson W. Crawford , Ravi Attri , Christopher A. Battles , Benjamin R. Bartfeld , Gregory R. Hires
CPC classification number: B01L3/50 , B01L3/502 , B01L3/50215 , B01L3/5082 , B01L3/50825 , B01L2300/06 , B01L2300/0636 , B01L2300/0663 , G01N1/40
Abstract: A method for detecting the presence of a microorganism in a fluid sample is disclosed. The method includes providing a fluid specimen within a specimen collection container having a sidewall defining an interior therein. The interior includes a mechanical separator adapted for separating the fluid sample into first and second phases within the specimen collection container and a sensing element capable of detecting the presence of a microorganism disposed therein. The method includes subjecting the specimen collection container to applied rotational force to isolate a concentrated microorganism region. The method also includes detecting by a sensing element the presence or absence of a microorganism within the concentrated microorganism region.
Abstract translation: 公开了一种用于检测流体样品中微生物的存在的方法。 该方法包括在具有限定其内部的侧壁的样本收集容器内提供流体样本。 内部包括适于将流体样品分离成样品收集容器内的第一和第二相的机械分离器和能够检测其中存在的微生物的感测元件。 该方法包括使样本收集容器施加旋转力以分离浓缩的微生物区域。 该方法还包括通过感测元件检测浓缩微生物区域内微生物的存在或不存在。
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