公开(公告)号：US09933341B2

公开(公告)日：2018-04-03

申请号：US14390596

申请日：2013-04-04

Applicant: Becton, Dickinson and Company

Inventor： Xiao Li ,  Yongqiang Zhang ,  Kenneth Anthony Kopher ,  John D. Mantlo ,  William Alfred Pope ,  Song Shi ,  Axel A. Yup

IPC: C12Q1/68 ,  G01N1/30 ,  C12Q1/06 ,  C12Q1/04

CPC classification number: G01N1/30 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2001/302

Abstract: Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.

公开(公告)号：US09645057B2

公开(公告)日：2017-05-09

申请号：US14774392

申请日：2013-04-04

Applicant: Becton, Dickinson and Company

Inventor： Xiao Li ,  Kenneth Anthony Kopher ,  John D. Mantlo ,  William Alfred Pope ,  Song Shi ,  Axel A. Yup

IPC: C12Q1/68 ,  G01N1/30 ,  C12Q1/06 ,  C12Q1/04

CPC classification number: G01N1/30 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2001/302

Abstract: A process for determining one of the presence, absence, or total of microorganisms (e.g. bacteria) in a sample. According to the process, a biological sample containing complex matrices is obtained. The sample is first combined with a resin to adsorb complex matrices from the sample. The resin is removed from the biological sample. The sample so prepared is then analyzed by flow cytometry.

公开(公告)号：US20160011087A1

公开(公告)日：2016-01-14

申请号：US14774392

申请日：2013-04-04

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Xiao Li ,  Kenneth Anthony Kopher ,  John D. Mantlo ,  William Alfred Pope ,  Song Shi ,  Axel A. Yup

IPC: G01N1/30 ,  C12Q1/04

CPC classification number: G01N1/30 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2001/302

Abstract: A process for determining one of the presence, absence, or total of microorganisms (e.g. bacteria) in a sample. According to the process, a biological sample containing complex matrices is obtained. The sample is first combined with a resin to adsorb complex matrices from the sample. The resin is removed from the biological sample. The sample so prepared is then analyzed by flow cytometry.

Abstract translation: 确定样品中微生物（例如细菌）的存在，不存在或总计之一的方法。 根据该方法，得到含有复合基质的生物样品。 样品首先与树脂组合以从样品中吸附复杂的基质。 从生物样品中除去树脂。 然后通过流式细胞术分析如此制备的样品。

公开(公告)号：US20150118677A1

公开(公告)日：2015-04-30

申请号：US14390596

申请日：2013-04-04

Applicant: Becton, Dickinson and Company

Inventor： Xiao Li ,  Yongqiang Zhang ,  Kenneth Anthony Kopher ,  John D. Mantlo ,  William Alfred Pope ,  Song Shi ,  Axel A. Yup

IPC: C12Q1/06 ,  G01N1/30

CPC classification number: G01N1/30 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2001/302

Abstract: Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.

Abstract translation: 本文描述了用于鉴定和分析样品中的至少一种微生物（例如细菌）的方法和试剂，并且通过流式细胞术分析样品时降低了获得的背景信号强度。 通过将样品与背景信号降低分子或与猝灭剂共价连接的核酸染色组合来制备样品。 在用核酸染色剂染色之前，可以用树脂去除样品中的一部分颗粒物质。