公开(公告)号：US20060258019A1

公开(公告)日：2006-11-16

申请号：US11461163

申请日：2006-07-31

申请人： Andrea Chow ,  Anne Kopf-Sill ,  J. Parce ,  Robert Dubrow

发明人： Andrea Chow ,  Anne Kopf-Sill ,  J. Parce ,  Robert Dubrow

IPC分类号： B01L3/00

CPC分类号： B01D61/18 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0457 ,  Y10T137/2496 ,  Y10T137/2499 ,  Y10T137/2501 ,  Y10T137/2514 ,  Y10T137/2516 ,  Y10T436/11 ,  Y10T436/2575

摘要： Methods and devices for delivering fluids into microfluidic device body structures are described. The methods and devices include the use of fluid manifolds that are integrated or interchangeable with device body structures. Methods of fabricating manifolds are also provided.

摘要翻译： 描述了将流体输送到微流体装置体结构中的方法和装置。 所述方法和装置包括使用与装置主体结构集成或可互换的流体歧管。 还提供制造歧管的方法。

公开(公告)号：US20060275817A1

公开(公告)日：2006-12-07

申请号：US11460550

申请日：2006-08-11

申请人： J. Parce ,  Theo Nikiforov ,  Tammy Mehta ,  Anne Kopf-Sill ,  Andrea Chow ,  Michael Knapp

发明人： J. Parce ,  Theo Nikiforov ,  Tammy Mehta ,  Anne Kopf-Sill ,  Andrea Chow ,  Michael Knapp

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6874 ,  B01F13/0059 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6869 ,  C40B60/14 ,  G01N27/44756 ,  G01N35/0098 ,  G01N2035/00574 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2535/101 ,  C12Q2563/173 ,  C12Q2525/186 ,  C12Q2527/125

摘要： Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.

摘要翻译： 核苷酸和核苷酸类似物通过合成方法的并入/测序用于各种测序。 包含3'-封闭基团的核苷酸类似物用于提供可逆的链终止，用于通过合成进行测序。 典型的封闭基团包括磷酸基和氨基甲酸酯基。 使用荧光核苷酸进行测序，通过掺入荧光标记的核苷酸进行检测，任选地随后进行光漂白，并使用嵌入染料检测通过插入检测合成测序中的未标记核苷酸的添加。 微流控器件，包括粒子阵列，用于测序方法。

公开(公告)号：US20070157973A1

公开(公告)日：2007-07-12

申请号：US11702368

申请日：2007-02-03

申请人： Ring-Ling Chien ,  J. Parce ,  Andrea Chow ,  Anne Kopf-Sill

发明人： Ring-Ling Chien ,  J. Parce ,  Andrea Chow ,  Anne Kopf-Sill

IPC分类号： F15C1/04 ,  F04B41/06

CPC分类号： B01L9/527 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N11/08 ,  G01N27/44791 ,  G01N2035/1044 ,  G05D7/0694 ,  Y10T137/2076 ,  Y10T137/218 ,  Y10T137/86131

摘要： Improved microfluidic devices, systems, and methods allow selective transportation of fluids within microfluidic channels of a microfluidic network by applying, controlling, and varying pressures at a plurality of reservoirs. Modeling the microfluidic network as a series of nodes connected together by channel segments and determining the flow resistance characteristics of the channel segments may allow calculation of fluid flows through the channel segments resulting from a given pressure configuration at the reservoirs. To effect a desired flow within a particular channel or series of channels, reservoir pressures may be identified using the network model. Viscometers or other flow sensors may measure flow characteristics within the channels, and the measured flow characteristics can be used to calculate pressures to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems can optionally be used in conjunction with electrokinetic or other fluid transport mechanisms.

公开(公告)号：US20050005684A1

公开(公告)日：2005-01-13

申请号：US10916270

申请日：2004-08-11

申请人： Ring-Ling Chien ,  J. Parce ,  Andrea Chow ,  Anne Kopf-Sill

发明人： Ring-Ling Chien ,  J. Parce ,  Andrea Chow ,  Anne Kopf-Sill

IPC分类号： G01N11/04 ,  B01L3/00 ,  B01L9/00 ,  G01N11/08 ,  G01N27/06 ,  G01N27/447 ,  G01N35/08 ,  G01N35/10 ,  G01N37/00 ,  G05D7/06 ,  G01N27/26

CPC分类号： B01L9/527 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N11/08 ,  G01N27/44791 ,  G01N2035/1044 ,  G05D7/0694 ,  Y10T137/2076 ,  Y10T137/218 ,  Y10T137/86131

摘要： Improved microfluidic devices, systems, and methods allow selective transportation of fluids within microfluidic channels of a microfluidic network by applying, controlling, and varying pressures at a plurality of reservoirs. Modeling the microfluidic network as a series of nodes connected together by channel segments and determining the flow resistance characteristics of the channel segments may allow calculation of fluid flows through the channel segments resulting from a given pressure configuration at the reservoirs. To effect a desired flow within a particular channel or series of channels, reservoir pressures may be identified using the network model. Viscometers or other flow sensors may measure flow characteristics within the channels, and the measured flow characteristics can be used to calculate pressures to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems can optionally be used in conjunction with electrokinetic or other fluid transport mechanisms.

摘要翻译： 改进的微流体装置，系统和方法允许通过在多个储存器处施加，控制和改变压力来选择性地输送微流体网络的微流体通道内的流体。 将微流体网络建模为通过通道段连接在一起的一系列节点并且确定通道段的流动阻力特性可以允许计算通过在储存器处的给定压力构造导致的通道段的流体流动。 为了在特定通道或一系列通道内实现期望的流量，可以使用网络模型来识别储层压力。 粘度计或其他流量传感器可以测量通道内的流量特性，并且可以使用测量的流量特性来计算产生期望流量的压力。 多储层压力调节器和压力控制器系统可以可选地与电动或其它流体输送机构结合使用。

公开(公告)号：US20080085521A1

公开(公告)日：2008-04-10

申请号：US11867626

申请日：2007-10-04

申请人： Michael Knapp ,  Jill Baker ,  Andrea Chow ,  Anne Kopf-Sill ,  Michael Spaid

发明人： Michael Knapp ,  Jill Baker ,  Andrea Chow ,  Anne Kopf-Sill ,  Michael Spaid

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851 ,  B01L3/5027 ,  B01L3/502761 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1833 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/6818 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N2021/6441 ,  C12Q2565/102 ,  C12Q2537/143 ,  C12Q2545/113 ,  C12Q2531/113 ,  C12Q2565/629 ,  C12Q2527/143

摘要： Methods and systems for performing single molecule amplification for detection, quantification and statistical analysis of nucleic acids are provided. Methods and systems are provided for determining and quantifying lengths of nucleic acids of interest.

摘要翻译： 提供了用于进行单分子扩增用于核酸检测，定量和统计分析的方法和系统。 提供了用于确定和定量感兴趣的核酸的长度的方法和系统。

公开(公告)号：US20080090244A1

公开(公告)日：2008-04-17

申请号：US11873753

申请日：2007-10-17

申请人： Michael Knapp ,  Jill Baker ,  Andrea Chow ,  Anne Kopf-Sill ,  Michael Spaid

发明人： Michael Knapp ,  Jill Baker ,  Andrea Chow ,  Anne Kopf-Sill ,  Michael Spaid

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/1827 ,  B01L2400/0406 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q2531/113 ,  C12Q2565/629 ,  C12Q2527/143 ,  C12Q2547/107 ,  C12Q2537/157

摘要： Methods are provided for detecting low copy nucleic acids of interest in a sample. In one method, a sample comprising a nucleic acid of interest is aliquotted into a plurality of reaction mixtures, at least two of which are single-copy reaction mixtures. The reaction mixtures are subjected to one or more amplification reactions while flowing through a channel of a microfluidic device. At least one of the reaction mixtures is formulated in an aqueous phase of an emulsion comprising aqueous droplets suspended in an immiscible liquid. The nucleic acid of interest is present as a single copy in at least one aqueous droplet of the aqueous phase prior to performing the amplification reaction(s). Amplification is performed on the reaction mixture when it is formulated in the emulsion. The nucleic acid is continuously flowed during a plurality of steps of the method.

摘要翻译： 提供了用于检测样品中感兴趣的低拷贝核酸的方法。 在一种方法中，将包含感兴趣的核酸的样品等分成多个反应混合物，其中至少两个是单拷贝反应混合物。 反应混合物在流过微流体装置的通道时进行一次或多次扩增反应。 将至少一种反应混合物配制在包含悬浮在不混溶液体中的水性液滴的乳液的水相中。 感兴趣的核酸在进行扩增反应之前以水相的至少一个水滴形式存在于单拷贝中。 当将其配制在乳液中时，对反应混合物进行扩增。 在该方法的多个步骤期间，核酸连续流动。

公开(公告)号：US20050153459A1

公开(公告)日：2005-07-14

申请号：US11005331

申请日：2004-12-06

申请人： Anne Kopf-Sill ,  Steven Sundberg ,  Andrea Chow ,  Claudia Poglitsch

发明人： Anne Kopf-Sill ,  Steven Sundberg ,  Andrea Chow ,  Claudia Poglitsch

IPC分类号： B01F13/00 ,  B01L3/00 ,  G01N1/38 ,  G01N27/447 ,  G01N1/00

CPC分类号： B01L3/5027 ,  B01F13/0059 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/049 ,  B01L2400/084 ,  G01N1/38 ,  G01N27/44791 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25625 ,  Y10T436/2575

摘要： Flow rates in a microfluidic device are modulated after performing serial dilutions by flow reduction channels that draw fluid from the main channel, thus reducing the flow rate. The reduction in flow rate and/or use of smaller dimension channels allow reduced reagent consumption. In addition, multiple flow reduction channels are used for multiple concentration measurements and for performing multiple assays simultaneously on a single sample. Also included are microfluidic devices and integrated systems for performing assays using serial dilutions, single pressure sources, multiple concentration measurements, and reduced reagent consumption. Devices comprising flow reduction channels are also used to suppress pressure perturbations from spontaneous injection.

公开(公告)号：US20050135655A1

公开(公告)日：2005-06-23

申请号：US11036722

申请日：2005-01-13

申请人： Anne Kopf-Sill ,  Andrea Chow ,  Peter Jann ,  Morten Jensen ,  Michael Spaid ,  Colin Kennedy ,  Michael Kennedy

发明人： Anne Kopf-Sill ,  Andrea Chow ,  Peter Jann ,  Morten Jensen ,  Michael Spaid ,  Colin Kennedy ,  Michael Kennedy

IPC分类号： B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  G01N21/05 ,  G01N21/64 ,  G01N27/447 ,  G01N35/00 ,  G01N37/00 ,  G01L9/18

CPC分类号： G01N21/6452 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/54 ,  B01L9/527 ,  B01L2200/023 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/147 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/645 ,  G01N27/44721 ,  G01N27/44782 ,  G01N35/0099 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421

摘要： Analytical systems and methods that use a modular interface structure for providing an interface between a sample substrate and an analytical unit, where the analytical unit typically has a particular interface arrangement for implementing various analytical and control functions. Using a number of variants for each module of the modular interface structure advantageously provides cost effective and efficient ways to perform numerous tests using a particular substrate or class of substrates with a particular analytical and control systems interface arrangement. Improved optical illumination and detection system for simultaneously analyzing reactions or conditions in multiple parallel microchannels are also provided. Increased throughput and improved emissions detection is provided by the present invention by simultaneously illuminating multiple parallel microchannels at a non-normal incidence using an excitation beam including multiple excitation frequencies, and simultaneously detecting emissions from the substances in the microchannels in a direction normal to the substrate using a detection module with multiple detectors.

摘要翻译： 分析系统和方法使用模块化接口结构来提供样品基底和分析单元之间的界面，其中分析单元通常具有用于实现各种分析和控制功能的特定界面布置。 使用模块化接口结构的每个模块的许多变体有利地提供了使用具有特定分析和控制系统接口布置的特定基板或类别的基板执行大量测试的成本有效和有效的方式。 还提供了用于同时分析多个并行微通道中的反应或条件的改进的光学照明和检测系统。 通过使用包括多个激发频率的激发束同时照射非法线入射的多个平行微通道并且同时在垂直于衬底的方向上检测来自微通道中的物质的发射，本发明提供了增加的吞吐量和改进的排放物检测 使用具有多个检测器的检测模块。

公开(公告)号：US20050042639A1

公开(公告)日：2005-02-24

申请号：US10845996

申请日：2004-05-14

申请人： Michael Knapp ,  Jill Baker ,  Andrea Chow ,  Anne Kopf-Sill ,  Michael Spaid

发明人： Michael Knapp ,  Jill Baker ,  Andrea Chow ,  Anne Kopf-Sill ,  Michael Spaid

IPC分类号： B01L3/00 ,  B01L7/00 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6851 ,  B01L3/5027 ,  B01L3/502761 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1833 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/6818 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N2021/6441 ,  C12Q2565/102 ,  C12Q2537/143 ,  C12Q2545/113 ,  C12Q2531/113 ,  C12Q2565/629 ,  C12Q2527/143

摘要： Methods and systems for performing single molecule amplification for detection, quantification and statistical analysis of nucleic acids are provided. Methods and systems are provided for determining and quantifying lengths of nucleic acids of interest.

摘要翻译： 提供了用于进行单分子扩增用于核酸检测，定量和统计分析的方法和系统。 提供了用于确定和定量感兴趣的核酸的长度的方法和系统。

公开(公告)号：US20050241941A1

公开(公告)日：2005-11-03

申请号：US11176805

申请日：2005-07-06

申请人： J. Parce ,  Anne Kopf-Sill ,  Luc Bousse

发明人： J. Parce ,  Anne Kopf-Sill ,  Luc Bousse

IPC分类号： G01N31/20 ,  B01J19/00 ,  B01L3/00 ,  C12Q1/04 ,  C12Q1/25 ,  G01N27/26 ,  G01N27/27 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G01N33/574 ,  G01N35/08 ,  G01N37/00

CPC分类号： G01N33/502 ,  B01J19/0093 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00952 ,  B01J2219/00961 ,  B01J2219/0097 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0487 ,  G01N27/44791 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/5064 ,  G01N33/5091 ,  G01N33/5097 ,  G01N33/5304 ,  G01N33/57415 ,  G01N35/08 ,  Y10S366/02 ,  Y10S435/81 ,  Y10S435/817 ,  Y10S436/804 ,  Y10S436/805 ,  Y10S436/806 ,  Y10T436/11 ,  Y10T436/110833 ,  Y10T436/113332 ,  Y10T436/117497 ,  Y10T436/25

摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.

摘要翻译： 本发明提供了可用于进行高通量筛选测定的新型微流体装置和方法。 特别地，本发明的装置和方法可用于筛选大量不同的化合物，因为它们对多种化学品，优选生物化学体系的影响。