公开(公告)号：US20130079247A1

公开(公告)日：2013-03-28

申请号：US13683214

申请日：2012-11-21

Applicant: SEQUENOM, INC.

Inventor： Xian-Wei YAO ,  Chao LIN ,  Paul HEANEY ,  Thomas BECKER ,  Aaron A. HANSON ,  Michael C. WILLIS

IPC: G01N1/10

CPC classification number: B01J19/0046 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/0244 ,  B01L3/0248 ,  B01L2200/0657 ,  B82Y30/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N1/10 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/10 ,  G01N35/1016 ,  G01N35/1065 ,  G01N2035/00158 ,  G01N2035/1037 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/119163 ,  Y10T436/24 ,  Y10T436/2575

Abstract: A slotted pin tool, a delivery system containing the pin tool, a substrate for use in the system and methods using the pin tool and system are provided. The slotted pin tool contains a plurality of pins having slotted ends designed to fit around each loci of material deposited on a surface, such as a microarray, without contacting any of the deposited material. Sample is delivered by contacting the pin tool with the surface; the amount delivered is proportional to the velocity of the pin tool as it contacts the surface or the velocity of the liquid when movement of the pin is halted.

Abstract translation: 提供了一种开槽针工具，包含销工具的输送系统，用于该系统的基板和使用该销工具和系统的方法。 开槽销工具包含多个销，其具有开槽端，设计成适合于沉积在诸如微阵列之类的表面上的每个材料位置，而不接触任何沉积材料。 通过将销工具与表面接触来传送样品; 当销的运动停止时，传递的量与销工具的接触表面的速度或液体的速度成比例。

公开(公告)号：US20130079242A1

公开(公告)日：2013-03-28

申请号：US13624386

申请日：2012-09-21

Applicant: The Arizona Board of Regents, A body Corporate

Inventor： Stephen Albert Johnston ,  Phillip Stafford

IPC: G01N33/53

CPC classification number: G01N33/543 ,  C40B40/10 ,  G01N33/53 ,  G01N33/54306 ,  G01N33/6845 ,  G01N33/6854

Abstract: The invention provides arrays of compound for use in profiling samples. The arrays include compounds bind to components of the samples at relatively low affinities. The avidity of compounds binding to components of the samples can be increased by forming arrays such that multivalent components of the samples (e.g., antibodies or cells) can bind to more than one molecule of a compound at the same time. When a sample is applied to an array under such conditions, the compounds of the array bind to component(s) of the sample with significantly different avidities generating a profile characteristic of the sample.

Abstract translation: 本发明提供用于分析样品的化合物阵列。 阵列包括以相对低的亲和力结合样品的组分的化合物。 可以通过形成阵列使得样品（例如，抗体或细胞）的多价成分能够同时结合多于一种化合物的分子来增加与样品组分结合的化合物的亲合力。 当在这样的条件下将样品应用于阵列时，阵列的化合物与样品的组分结合，具有显着不同的avidities，产生样品的特性曲线。

公开(公告)号：US08399384B2

公开(公告)日：2013-03-19

申请号：US13161441

申请日：2011-06-15

Applicant: Robert Charles Ladner

Inventor： Robert Charles Ladner

IPC: C40B40/02 ,  C40B40/08

CPC classification number: C12N15/1037 ,  C07K16/00 ,  C07K16/005 ,  C07K2317/565 ,  C40B40/02 ,  C40B40/08 ,  C40B40/10

Abstract: Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies.

公开(公告)号：US08361933B2

公开(公告)日：2013-01-29

申请号：US12563897

申请日：2009-09-21

Applicant: Patrick S. Daugherty ,  Paul H. Bessette ,  Jeffrey Rice

Inventor： Patrick S. Daugherty ,  Paul H. Bessette ,  Jeffrey Rice

IPC: C40B40/02

CPC classification number: C12N15/1037 ,  C07K14/245 ,  C12N15/1044 ,  C40B30/04 ,  C40B40/10 ,  G01N33/554

Abstract: Disclosed herein are expression vectors which display a passenger polypeptide on the outer surface of a biological entity. As disclosed herein the displayed passenger polypeptide is capable of interacting or binding with a given ligand. Also disclosed are methods of making and using the expression vectors. N/C terminal fusion expression vectors and methods of making and using are also disclosed.

公开(公告)号：US08313940B2

公开(公告)日：2012-11-20

申请号：US11963317

申请日：2007-12-21

Applicant: Michael J. Heller ,  Eugene Tu

Inventor： Michael J. Heller ,  Eugene Tu

IPC: C12M1/36 ,  G01N15/06 ,  G01N27/00

CPC classification number: B82Y10/00 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00713 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01L3/502707 ,  B01L3/5085 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2400/0421 ,  B82Y5/00 ,  C07B2200/11 ,  C07H21/00 ,  C07K1/04 ,  C07K1/045 ,  C07K1/047 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B60/14 ,  G11C13/0014 ,  G11C13/0019 ,  G11C19/00 ,  H01L24/95 ,  H01L25/50 ,  H01L2924/10253 ,  H01L2924/12043 ,  C12Q2565/515 ,  C12Q2565/607 ,  H01L2924/00

Abstract: A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridization, antibody/antigen reaction, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Abstract translation: 设计制造了一种自寻址的自组装微电子器件，主动执行和控制微步形式的多步分子生物反应。 这些反应包括核酸杂交，抗体/抗原反应，诊断和生物聚合物合成。 该器件可以使用微光刻和微加工技术制造。 该设备可以电子控制特定绑定实体到特定微位置的传输和附着。 特异性结合实体包括分子生物学分子，例如核酸和多肽。 该装置随后可以控制分析物或反应物在寻址的特定微位置的转运和反应。 该装置能够浓缩分析物和反应物，去除非特异性结合的分子，为DNA杂交反应提供严格控制，并改善分析物的检测。 该设备可以电子复制。

公开(公告)号：US20120238466A1

公开(公告)日：2012-09-20

申请号：US13478689

申请日：2012-05-23

Applicant: Michael P. Nova ,  Andrew E. Senyei ,  Zahra Parandoosh ,  Gary S. David ,  Xiao-Yi Xiao

Inventor： Michael P. Nova ,  Andrew E. Senyei ,  Zahra Parandoosh ,  Gary S. David ,  Xiao-Yi Xiao

IPC: C40B30/04

CPC classification number: C40B30/04 ,  B01J19/0046 ,  B01J2219/00295 ,  B01J2219/00299 ,  B01J2219/00308 ,  B01J2219/00333 ,  B01J2219/00344 ,  B01J2219/00461 ,  B01J2219/00463 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/0054 ,  B01J2219/00542 ,  B01J2219/00547 ,  B01J2219/00549 ,  B01J2219/00551 ,  B01J2219/0056 ,  B01J2219/00563 ,  B01J2219/00567 ,  B01J2219/00569 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/5027 ,  B01L3/502707 ,  B01L2300/0609 ,  B82Y10/00 ,  C07K1/00 ,  C07K1/04 ,  C07K1/047 ,  C40B40/06 ,  C40B40/10 ,  C40B60/08 ,  C40B60/14 ,  C40B70/00 ,  G01N35/00732 ,  G01N35/00871 ,  G01N2015/149 ,  G01N2035/00782 ,  G11C13/0014 ,  G11C13/0019 ,  G11C13/025

Abstract: In a multiplexed assay, each molecule of a plurality of molecules is attached to a support matrix with a substrate adapted for attachment and/or synthesis of molecules and an integrally-formed memory device with an optically-encoded identifier to uniquely identify the molecule attached to the substrate. The molecules are exposed to one or more processing conditions then placed within the path of an optical detector adapted to read the optically-encoded identifier and measure biochemical processes on each support matrix. The support matrices may be singulated to be read by the optical detector one at a time.

Abstract translation: 在多重分析中，多个分子的每个分子连接到具有适于附着和/或合成分子的底物的支持基质和具有光学编码标识符的整体形成的存储器件，以唯一地识别附着于 底物。 分子暴露于一个或多个处理条件，然后放置在适于读取光学编码标识符并测量每个支撑矩阵上的生物化学过程的光学检测器的路径内。 支持矩阵可以一次被光学检测器单独读取。

公开(公告)号：US20120228386A1

公开(公告)日：2012-09-13

申请号：US13476809

申请日：2012-05-21

Applicant: Lei WU ,  Xiaobo Wang ,  Guoliang Tao ,  Junquan Xu ,  Jing Cheng ,  Mingxian Huang ,  Baoquan Sun ,  Wei Shao ,  Litian Liu ,  Depu Chen ,  David M. Rothwarf ,  Weiping Yang

Inventor： Lei WU ,  Xiaobo Wang ,  Guoliang Tao ,  Junquan Xu ,  Jing Cheng ,  Mingxian Huang ,  Baoquan Sun ,  Wei Shao ,  Litian Liu ,  Depu Chen ,  David M. Rothwarf ,  Weiping Yang

IPC: G06K19/00 ,  G06K19/06 ,  G06K19/02

CPC classification number: C40B40/12 ,  B01J19/0046 ,  B01J2219/00457 ,  B01J2219/00468 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/0054 ,  B01J2219/00542 ,  B01J2219/00547 ,  B01J2219/00549 ,  B01J2219/00556 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00655 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00707 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  B01J2219/00734 ,  B01J2219/0074 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2400/0415 ,  B01L2400/043 ,  B03C5/028 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  C07B2200/11 ,  C07K1/047 ,  C40B40/06 ,  C40B40/10 ,  G01N33/54366 ,  G01N33/54386 ,  G06K19/06009 ,  G06K19/06046 ,  G06K19/06196 ,  Y10T428/24917

Abstract: This invention relates generally to the field of moiety or molecule analysis, isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a substrate; and b) a photorecognizable coding pattern on said substrate. Preferably, the microdevice does not comprise an anodized metal surface layer. Methods and kits for isolating, detecting and manipulating moieties, and synthesizing libraries using the microdevices are also provided. The invention further provides two-dimensional optical encoders and uses thereof. In certain embodiments, the invention provides a microdevice, which microdevice comprises: a) a magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating, detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided.

Abstract translation: 本发明一般涉及部分或分子分析，分离，检测和操作以及文库合成领域。 特别地，本发明提供了一种微型器件，该微型器件包括：a）衬底; 和b）在所述衬底上的可识别的编码图案。 优选地，微器件不包括阳极化金属表面层。 还提供了用于分离，检测和操作部分以及使用微器件合成文库的方法和试剂盒。 本发明还提供二维光学编码器及其用途。 在某些实施方案中，本发明提供一种微型装置，该微型装置包括：a）可磁化物质; 以及b）可识别的编码图案，其中所述微器件具有优先磁化轴。 还提供了用于使用微器件分离，检测和操作部分和合成文库的系统和方法。

公开(公告)号：US20120142541A1

公开(公告)日：2012-06-07

申请号：US13147257

申请日：2010-02-04

Applicant: Gregory Paul Winter ,  Christian Heinis ,  Elise Bernard ,  David Loakes ,  Marina Vaysburd

Inventor： Gregory Paul Winter ,  Christian Heinis ,  Elise Bernard ,  David Loakes ,  Marina Vaysburd

IPC: C40B30/00 ,  C40B40/10

CPC classification number: C12N15/1037 ,  A61K47/55 ,  A61K47/64 ,  A61K47/643 ,  C07K14/001 ,  C12N15/1044 ,  C12N15/1058 ,  C40B40/08 ,  C40B40/10 ,  C40B50/06 ,  G01N33/531

Abstract: The invention relates to a method for modifying one or more peptide ligands, comprising polypeptides covalently linked to a molecular scaffold at two or more amino acid residues, comprising the steps of providing one or more peptide ligands, wherein the polypeptide comprises two or more reactive groups which form a covalent linkage to the molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; exposing the peptide ligands to one or more proteases; and sorting the ligands according to the extent of proteolytic cleavage.

Abstract translation: 本发明涉及一种修饰一种或多种肽配体的方法，其包括在两个或更多个氨基酸残基处与分子支架共价连接的多肽，包括提供一个或多个肽配体的步骤，其中所述多肽包含两个或多个反应性基团 其形成与分子支架的共价键，以及至少一个环，其包含在两个所述反应性基团之间对位的两个或更多个氨基酸的序列; 将肽配体暴露于一种或多种蛋白酶; 并根据蛋白水解切割的程度对配体进行分选。

公开(公告)号：US20120123098A1

公开(公告)日：2012-05-17

申请号：US13374202

申请日：2011-12-14

Applicant: Bhatt Ramesh ,  Lawrence Horowitz ,  Li Xu

Inventor： Bhatt Ramesh ,  Lawrence Horowitz ,  Li Xu

IPC: C07K19/00

CPC classification number: C07K16/00 ,  C07K16/005 ,  C07K16/1018 ,  C07K16/18 ,  C07K16/22 ,  C07K16/241 ,  C07K2317/34 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2319/00 ,  C40B40/10

Abstract: The invention concerns constructs and libraries comprising antibody surrogate light chain sequences. In particular, the invention concerns constructs comprising VpreB sequences, optionally partnered with another polypeptide, such as, for example, antibody heavy chain variable domain sequences, and libraries containing the same.

Abstract translation: 本发明涉及包含抗体替代轻链序列的构建体和文库。 特别地，本发明涉及包含任选与另一多肽例如抗体重链可变结构域序列组合的VpreB序列的构建体和含有该多肽的文库。

公开(公告)号：US08178652B2

公开(公告)日：2012-05-15

申请号：US13168131

申请日：2011-06-24

Applicant: Michal Lebl

Inventor： Michal Lebl

IPC: A61K33/00

CPC classification number: C07K1/045 ,  B01D17/0202 ,  B01D17/0217 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00326 ,  B01J2219/00351 ,  B01J2219/00421 ,  B01J2219/00466 ,  B01J2219/0049 ,  B01J2219/005 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00599 ,  B01J2219/00605 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B04B5/0407 ,  B04B5/0421 ,  C07K1/04 ,  C40B40/06 ,  C40B40/10 ,  C40B50/08 ,  C40B60/14 ,  G01N35/028 ,  G01N2035/00495

Abstract: A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis or purification (extraction). In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.

Abstract translation: 公开了一种简单有效的装置和方法，用于分离用于高通量组合有机合成或平行提取大型文库或有机化合物的大量阵列的方法中的不混溶或部分混溶液体层。 该装置和方法无论作为组合有机合成或纯化（提取）的自动机器人或手动系统的一部分是有用的。 在优选实施例中，公开了用于分离与微量滴定板型阵列的反应容器相容的不混溶或部分可混溶液体的层的装置和方法。 发现基于离心的液体去除的另一种应用是在生物测定中进行洗涤或在改性底物上进行合成。