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    Serial-line-scan-encoded multi-color fluorescence microscopy and imaging flow cytometry
    71.
    发明授权
    Serial-line-scan-encoded multi-color fluorescence microscopy and imaging flow cytometry 有权
    串行扫描编码的多色荧光显微镜和成像流式细胞术

    公开(公告)号：US08639012B2

    公开(公告)日：2014-01-28

    申请号：US12726628

    申请日：2010-03-18

    申请人： Xin Heng ,  Paul Patt

    发明人： Xin Heng ,  Paul Patt

    IPC分类号： G06K9/00

    CPC分类号： G01N21/6486 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/04 ,  G01J3/10 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/1425 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421

    摘要： A system for performing high-speed, high-resolution imaging cytometry utilizes a line-scan sensor. A cell to be characterized is transported past a scan region. An optical system focuses an image of a portion of the scan region onto at least one linear light sensor, and repeated readings of light falling on the sensor are taken while a cell is transported though the scan region. The system may image cells directly, or may excite fluorescence in the cells and image the resulting light emitted from the cell by fluorescence. The system may provide a narrow band of illumination at the scan region. The system may include various filters and imaging optics that enable simultaneous multicolor fluorescence imaging cytometry. Multiple linear sensors may be provided, and images gathered by the individual sensors may be combined to construct an image having improved signal-to-noise characteristics.

    摘要翻译： 用于执行高速，高分辨率成像细胞计数的系统利用线扫描传感器。 待特征化的细胞被运送通过扫描区域。 光学系统将扫描区域的一部分的图像聚焦到至少一个线性光传感器上，并且在单元格被传送通过扫描区域的同时拍摄落在传感器上的光的重复读数。 该系统可以直接对细胞进行成像，或者可以激发细胞中的荧光，并通过荧光对从细胞发出的所得光进行成像。 该系统可以在扫描区域提供窄带照明。 该系统可以包括能够同时进行多色荧光成像细胞计数的各种滤光器和成像光学元件。 可以提供多个线性传感器，并且可以组合由各个传感器收集的图像以构建具有改进的信噪比特性的图像。

    DEVICE AND METHOD FOR MULTIPARAMETRIC MEASUREMENTS OF MICROPARTICLES IN A FLUID
    74.
    发明申请
    DEVICE AND METHOD FOR MULTIPARAMETRIC MEASUREMENTS OF MICROPARTICLES IN A FLUID 有权
    流体中微量元素多元测量的装置和方法

    公开(公告)号：US20130308122A1

    公开(公告)日：2013-11-21

    申请号：US13983082

    申请日：2011-11-15

    申请人： Benoit Merchez ,  Didier Chemien ,  Alexandra Urankar

    发明人： Benoit Merchez ,  Didier Chemien ,  Alexandra Urankar

    IPC分类号： G01N21/47 ,  G01N21/64 ,  G01N27/04

    CPC分类号： G01N21/47 ,  G01N15/1434 ,  G01N15/147 ,  G01N21/51 ,  G01N21/64 ,  G01N21/645 ,  G01N27/04 ,  G01N2015/1037

    摘要： The invention relates to an electro-optical device for measurements of flow for the characterization of microparticles, comprising a measurement chamber (CUM) in which there circulates the flow of the fluid to be characterized, at least two luminous sources (S1,S2) of disjoint spectra, a device for measuring resistivity (RES), and at least three other detectors (D1,D2,D3) each allowing the measurement of an optical parameter, the optical parameters being chosen from among fluorescence (FL), extinction (EXT), wide angle diffraction (SSC) and small angle diffraction (FSC).

    摘要翻译： 本发明涉及一种用于测量微粒表征的流动的电光装置，包括测量室（CUM），其中使要表征的流体的流动循环至少两个光源（S1，S2），其中， 分离光谱，用于测量电阻率（RES）的装置和至少三个其他检测器（D1，D2，D3），每个允许测量光学参数，光学参数选自荧光（FL），消光（EXT） ，广角衍射（SSC）和小角度衍射（FSC）。

    Detection of circulating tumor cells using imaging flow cytometry
    75.
    发明授权
    Detection of circulating tumor cells using imaging flow cytometry 有权
    使用成像流式细胞术检测循环肿瘤细胞

    公开(公告)号：US08548219B2

    公开(公告)日：2013-10-01

    申请号：US13396333

    申请日：2012-02-14

    申请人： William Ortyn ,  David Basiji ,  Luchuan Liang ,  Vidya Venkatachalam ,  Philip Morrissey

    发明人： William Ortyn ,  David Basiji ,  Luchuan Liang ,  Vidya Venkatachalam ,  Philip Morrissey

    IPC分类号： G06K9/00 ,  G01J3/28

    CPC分类号： G01N15/147 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5005 ,  G01N33/505 ,  G01N2021/6441

    摘要： Photometric and morphometric features derived from multi-mode imagery of cells in flow are used as a cell analyzer to determine if a marker corresponding to a cancer cell or precancerous cell is present in the population of cells imaged. An imaging system simultaneously acquires a plurality of images for each cell passing through the field of view of the imaging system. Acquiring a plurality of different images (i.e., bright field, dark field, and fluorescent images) facilitates the determination of different morphological and morphometric parameters. Simultaneously acquiring the plurality of images enables relatively large populations of cells to be rapidly imaged, so that relatively small numbers of cancer cells in a large population of cells can be detected. Initially, known cancer cells are imaged to enable a marker to be identified. Then, a sample that may include cancer cells is imaged to determine if the marker is present.

    摘要翻译： 作为细胞分析仪，使用从流动细胞的多模式图像得到的光度和形态测定特征，以确定对应于癌细胞或癌前细胞的标志物是否存在于成像细胞群体中。 成像系统同时获取通过成像系统的视野的每个单元的多个图像。 获取多个不同的图像（即，亮场，暗场和荧光图像）有助于确定不同的形态和形态测量参数。 同时获取多个图像使得能够快速成像相对较大数量的细胞，从而可以检测大量细胞中相对少数量的癌细胞。 首先，将已知的癌细胞成像以使得能够鉴定标记。 然后，可以包含癌细胞的样品被成像以确定标记物是否存在。

    METHODS AND APPARATUS FOR CLASSIFICATION AND QUANTIFICATION OF MULTIFUNCTIONAL OBJECTS
    76.
    发明申请
    METHODS AND APPARATUS FOR CLASSIFICATION AND QUANTIFICATION OF MULTIFUNCTIONAL OBJECTS 审中-公开
    多功能对象的分类和量化方法与装置

    公开(公告)号：US20130244909A1

    公开(公告)日：2013-09-19

    申请号：US13790662

    申请日：2013-03-08

    申请人： FIREFLY BIOWORKS, INC.

    发明人： Andreas Windemuth ,  Daniel Pregibon ,  Davide Marini

    IPC分类号： G01N33/50 ,  G06M3/08

    CPC分类号： G01N33/50 ,  G01N15/1427 ,  G01N15/1429 ,  G01N15/1459 ,  G01N15/147 ,  G01N35/00732 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  G06M3/08

    摘要： A method may include accessing data regarding a number of events, where the events were detected by a particle detection apparatus, and identifying a number of groups in the events. Each of the groups includes two or more events, and each event includes one or more of a time stamp, a time duration, a fluorescence intensity, a scatter measurement, a radio frequency signal, a magnetic signal, a neutron scattering measurement, a light scattering measurement, an electron scattering measurement, an audio signal, an acoustic signal, a mechanical signal, an electrical resistance, a thermal property, and a width of fluorescence signal. The method may include identifying a subset of the groups as a number of objects detected by the particle detection apparatus, where each object is identified based at least in part upon one or more quantities, where each quantity is identified by or derived from the respective two or more events.

    摘要翻译： 方法可以包括访问关于事件的数量的数据，其中事件由粒子检测装置检测到，并且识别事件中的组的数量。 每个组包括两个或更多个事件，并且每个事件包括时间戳，持续时间，荧光强度，散射测量，射频信号，磁信号，中子散射测量，光 散射测量，电子散射测量，音频信号，声学信号，机械信号，电阻，热性质和荧光信号的宽度。 所述方法可以包括将所述组的子集识别为由所述粒子检测装置检测到的多个对象，其中每个对象至少部分地基于一个或多个数量来识别，其中每个数量由相应的两个 或更多的事件。

    EFFICIENT HAPLOID CELL SORTING FLOW CYTOMETER SYSTEMS
    77.
    发明申请
    EFFICIENT HAPLOID CELL SORTING FLOW CYTOMETER SYSTEMS 审中-公开
    有效的HAPLOID细胞分流流式细胞仪系统

    公开(公告)号：US20130224843A1

    公开(公告)日：2013-08-29

    申请号：US13764548

    申请日：2013-02-11

    申请人： XY, LLC

    发明人： Kenneth M. Evans ,  Thomas B. Gillilgan

    IPC分类号： C12Q3/00

    CPC分类号： C12Q3/00 ,  C12N5/0612 ,  G01N15/147 ,  G01N15/1475 ,  G01N33/5005 ,  G01N2015/149 ,  Y10T436/12

    摘要： A flow cytometry system (1) for sorting haploid cells, specifically irradiatable sperm cells, with an intermittingly punctuated radiation emitter (56). Embodiments include a beam manipulator (21) and even split radiation beams directed to multiple nozzles (5). Differentiation of sperm characteristics with increased resolution may efficiently allow differentiated sperm cells to be separated higher speeds and even into subpopulations having higher purity.

    摘要翻译： 一种流式细胞术系统（1），用于将单倍体细胞（特别是可照射的精子细胞）与中间标记的辐射发射器（56）分离。 实施例包括光束操纵器（21）以及指向多个喷嘴（5）的均匀的分束辐射束。 精子特征与分辨率的分化可以有效地使分化精子细胞被分离得更高，甚至可以分离出具有较高纯度的亚群。

    FLOW CYTOMETER METHOD AND APPARATUS
    78.
    发明申请
    FLOW CYTOMETER METHOD AND APPARATUS 有权
    流量计方法和装置

    公开(公告)号：US20130224787A1

    公开(公告)日：2013-08-29

    申请号：US13782459

    申请日：2013-03-01

    申请人： INGURAN, LLC

    发明人： Gary Durack ,  Jeffrey D. Wallace ,  Gary P. Vandre ,  Lon A. Westfall ,  Jeremy T. Hatcher ,  Niraj V. Nayak

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0612 ,  A01N1/0284 ,  C12N5/06 ,  C12N5/061 ,  C12Q1/02 ,  C12Q3/00 ,  G01N15/1468 ,  G01N15/147 ,  G01N21/63 ,  G01N21/6428 ,  G01N33/48 ,  G01N33/5005 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2021/6439 ,  Y10T436/10 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/25 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

    摘要： A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.

    摘要翻译： 一种用于根据一种或多种颗粒特性分级颗粒的多通道装置。 该装置包括多个流式细胞术单元，每个流式细胞术单元可操作以通过用电磁辐射束询问含有颗粒的流体流来分类颗粒混合物中的颗粒。 流式细胞术单元共享包括以下至少一个的综合平台：（1）通常的颗粒供应; （2）共同住房; （3）用于控制单元的操作的通用处理器; （4）用于从各单元接收和处理信息的通用处理器; 和（5）普通流体输送系统。 集成平台可以包括电磁辐射的共同来源。 本发明的方法包括使用共享整合平台的多个流式细胞仪单元来进行诸如分析或分选颗粒的流量计操作。

    SYSTEMS AND METHODS FOR SORTING PARTICLES
    79.
    发明申请
    SYSTEMS AND METHODS FOR SORTING PARTICLES 有权
    用于分离颗粒的系统和方法

    公开(公告)号：US20130224727A1

    公开(公告)日：2013-08-29

    申请号：US13762014

    申请日：2013-02-07

    申请人： INGURAN, LLC

    发明人： Gary Durack ,  Jeffrey D. Wallace ,  Gary P. Vandre ,  Lon A. Westfall ,  Jeremy T. Hatcher ,  Niraj V. Nayak

    IPC分类号： C12Q3/00

    CPC分类号： C12N5/0612 ,  A01N1/0284 ,  C12N5/06 ,  C12N5/061 ,  C12Q1/02 ,  C12Q3/00 ,  G01N15/1468 ,  G01N15/147 ,  G01N21/63 ,  G01N21/6428 ,  G01N33/48 ,  G01N33/5005 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2021/6439 ,  Y10T436/10 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/25 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

    摘要： A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.

    摘要翻译： 一种用于根据一种或多种颗粒特性分级颗粒的多通道装置。 该装置包括多个流式细胞术单元，每个流式细胞术单元可操作以通过用电磁辐射束询问含有颗粒的流体流来分类颗粒混合物中的颗粒。 流式细胞术单元共享包括以下至少一个的综合平台：（1）通常的颗粒供应; （2）共同住房; （3）用于控制单元的操作的通用处理器; （4）用于从各单元接收和处理信息的通用处理器; 和（5）普通流体输送系统。 集成平台可以包括电磁辐射的共同来源。 本发明的方法包括使用共享整合平台的多个流式细胞仪单元来进行诸如分析或分选颗粒的流量计操作。