公开(公告)号：US11104880B2

公开(公告)日：2021-08-31

申请号：US16155576

申请日：2018-10-09

申请人： Inguran, LLC

发明人： Gary Durack ,  Jeffrey D. Wallace ,  Gary P. Vandre ,  Lon A. Westfall ,  Jeremy T. Hatcher ,  Niraj V. Nayak

IPC分类号： G01N33/50 ,  G01N33/48 ,  G01N21/64 ,  G01N15/14 ,  C12Q1/02 ,  C12N5/076 ,  C12N5/071 ,  A01N1/02 ,  C12Q3/00 ,  C12N5/07 ,  G01N21/63 ,  G01N15/00 ,  G01N15/10

摘要： A system for sorting a mixture of sperm cells in a fluid flow path, including stained sperm cells from two distinguishable groups, namely X chromosome bearing sperm and Y chromosome bearing sperm. The system and method can include an electromagnetic radiation source for exciting fluorescence emissions from the stained particles, a photodetector for detecting the fluorescence emissions from the stained particles, a processor for classifying the stained particles; and a photo-damaging laser for damaging selected particles in the flow path.

公开(公告)号：US10100278B2

公开(公告)日：2018-10-16

申请号：US15179722

申请日：2016-06-10

申请人： Inguran, LLC

发明人： Gary Durack ,  Jeffrey D. Wallace ,  Gary P. Vandre ,  Lon A. Westfall ,  Jeremy T. Hatcher ,  Niraj V. Nayak

IPC分类号： C12N5/071 ,  C12N5/076 ,  G01N15/14 ,  G01N21/64 ,  C12Q1/02 ,  C12Q3/00 ,  G01N33/48 ,  G01N33/50 ,  A01N1/02 ,  G01N15/00 ,  G01N15/10

摘要： A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.

公开(公告)号：US20140220620A1

公开(公告)日：2014-08-07

申请号：US14206832

申请日：2014-03-12

申请人： INGURAN, LLC

发明人： Gary Durack ,  Jeffrey D. Wallace ,  Gary P. Vandre ,  Lon A. Westfall ,  Jeremy T. Hatcher ,  Niraj V. Nayak

IPC分类号： G01N33/50

CPC分类号： C12N5/0612 ,  A01N1/0284 ,  C12N5/06 ,  C12N5/061 ,  C12Q1/02 ,  C12Q3/00 ,  G01N15/1468 ,  G01N15/147 ,  G01N21/63 ,  G01N21/6428 ,  G01N33/48 ,  G01N33/5005 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2021/6439 ,  Y10T436/10 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/25 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： A multi-channel system and methods for sorting particles according to one or more characteristics of the particles. The system includes multiple flow cytometry units, each unit can have a nozzle for producing a fluid stream containing a desired population of particles in a mixture of particles. Each of the units may be operable to sort said desired population of particles by interrogating the fluid stream with a beam of electromagnetic radiation and classifying particles based on one or more characteristics of the particles. The system also includes a common fluid delivery system for delivering sheath fluid to each flow cytometer unit for producing respective fluid streams.

摘要翻译： 一种多通道系统和根据颗粒的一个或多个特征分选颗粒的方法。 该系统包括多个流式细胞术单元，每个单元可以具有用于产生在颗粒混合物中含有所需粒子群的流体流的喷嘴。 每个单元可以可操作以通过用电磁辐射束询问流体流并基于颗粒的一个或多个特性对颗粒进行分类来对所需的颗粒群进行分类。 该系统还包括用于将鞘液输送到每个流式细胞仪单元以产生相应流体流的常用流体输送系统。

公开(公告)号：US08709825B2

公开(公告)日：2014-04-29

申请号：US13782459

申请日：2013-03-01

申请人： Inguran, LLC

发明人： Gary Durack ,  Jeffrey D. Wallace ,  Gary P. Vandre ,  Lon A. Westfall ,  Jeremy T. Hatcher ,  Niraj V. Nayak

IPC分类号： G01N1/10 ,  G01N1/14 ,  G01N33/48

CPC分类号： C12N5/0612 ,  A01N1/0284 ,  C12N5/06 ,  C12N5/061 ,  C12Q1/02 ,  C12Q3/00 ,  G01N15/1468 ,  G01N15/147 ,  G01N21/63 ,  G01N21/6428 ,  G01N33/48 ,  G01N33/5005 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2021/6439 ,  Y10T436/10 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/25 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： A nozzle for use in a flow cytometer and a method of orienting particles with a baffle disposed within a nozzle. The baffle may be a baffle plate positioned in a fluid flow path to deflect a fluid stream and to orient particles in a desired orientation as they exit the nozzle.

摘要翻译： 用于流式细胞计数器的喷嘴和设置在喷嘴内的带有挡板的颗粒定向的方法。 挡板可以是定位在流体流动路径中的挡板，以偏转流体流并且当它们离开喷嘴时以所需取向定向颗粒。

公开(公告)号：US08691584B2

公开(公告)日：2014-04-08

申请号：US13776379

申请日：2013-02-25

申请人： Inguran, LLC

发明人： Gary Durack ,  Jeffrey D. Wallace ,  Gary P. Vandre ,  Lon A. Westfall ,  Jeremy T. Hatcher ,  Niraj V. Nayak

IPC分类号： G01N33/48 ,  C12N5/076 ,  C12Q1/02 ,  G01N21/63 ,  C12N5/07

CPC分类号： C12N5/0612 ,  A01N1/0284 ,  C12N5/06 ,  C12N5/061 ,  C12Q1/02 ,  C12Q3/00 ,  G01N15/1468 ,  G01N15/147 ,  G01N21/63 ,  G01N21/6428 ,  G01N33/48 ,  G01N33/5005 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2021/6439 ,  Y10T436/10 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/25 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： A method of analyzing particles in a fluid stream including focusing a generally elliptical beam spot to a width less than the size of the particles. As particles pass through the beam spot electromagnetic radiation from the particles which is then detected and converted into electrical signals indicative of characteristics of the particles. The electrical signals may be digitally sampled to provide digital information which may be analyzed to extract information and to classify the particles.

摘要翻译： 一种分析流体流中的颗粒的方法，包括将基本上椭圆形的光束点聚焦到小于颗粒尺寸的宽度。 当颗粒通过来自颗粒的束斑电磁辐射时，其被检测并转换成指示颗粒特征的电信号。 电信号可以被数字采样以提供数字信息，其可以被分析以提取信息并对颗粒进行分类。

公开(公告)号：US20130224786A1

公开(公告)日：2013-08-29

申请号：US13782417

申请日：2013-03-01

申请人： INGURAN, LLC

发明人： Gary Durack ,  Jeffrey D. Wallace ,  Gary P. Vandre ,  Lon A. Westfall ,  Jeremy T. Hatcher ,  Niraj V. Nayak

IPC分类号： C12N5/071

CPC分类号： C12N5/0612 ,  A01N1/0284 ,  C12N5/06 ,  C12N5/061 ,  C12Q1/02 ,  C12Q3/00 ,  G01N15/1468 ,  G01N15/147 ,  G01N21/63 ,  G01N21/6428 ,  G01N33/48 ,  G01N33/5005 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2021/6439 ,  Y10T436/10 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/25 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.

摘要翻译： 一种用于根据一种或多种颗粒特性分级颗粒的多通道装置。 该装置包括多个流式细胞术单元，每个流式细胞术单元可操作以通过用电磁辐射束询问含有颗粒的流体流来分类颗粒混合物中的颗粒。 流式细胞术单元共享包括以下至少一个的综合平台：（1）通常的颗粒供应; （2）共同住房; （3）用于控制单元的操作的通用处理器; （4）用于从各单元接收和处理信息的通用处理器; 和（5）普通流体输送系统。 集成平台可以包括电磁辐射的共同来源。 本发明的方法包括使用共享整合平台的多个流式细胞仪单元来进行诸如分析或分选颗粒的流量计操作。

公开(公告)号：US20130224726A1

公开(公告)日：2013-08-29

申请号：US13762003

申请日：2013-02-07

申请人： INGURAN, LLC

发明人： Gary Durack ,  Jeffrey D. Wallace ,  Gary P. Vandre ,  Lon A. Westfall ,  Jeremy T. Hatcher ,  Niraj V. Nayak

IPC分类号： C12N5/071

CPC分类号： C12N5/0612 ,  A01N1/0284 ,  C12N5/06 ,  C12N5/061 ,  C12Q1/02 ,  C12Q3/00 ,  G01N15/1468 ,  G01N15/147 ,  G01N21/63 ,  G01N21/6428 ,  G01N33/48 ,  G01N33/5005 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2021/6439 ,  Y10T436/10 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/25 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.

摘要翻译： 一种用于根据一种或多种颗粒特性分级颗粒的多通道装置。 该装置包括多个流式细胞术单元，每个流式细胞术单元可操作以通过用电磁辐射束询问含有颗粒的流体流来分类颗粒混合物中的颗粒。 流式细胞术单元共享包括以下至少一个的综合平台：（1）通常的颗粒供应; （2）共同住房; （3）用于控制单元的操作的通用处理器; （4）用于从各单元接收和处理信息的通用处理器; 和（5）普通流体输送系统。 集成平台可以包括电磁辐射的共同来源。 本发明的方法包括使用共享整合平台的多个流式细胞仪单元来进行诸如分析或分选颗粒的流量计操作。

公开(公告)号：US20190040356A1

公开(公告)日：2019-02-07

申请号：US16155576

申请日：2018-10-09

申请人： Inguran, LLC

发明人： Gary Durack ,  Jeffrey D. Wallace ,  Gary P. Vandre ,  Lon A. Westfall ,  Jeremy T. Hatcher ,  Niraj V. Nayak

IPC分类号： C12N5/071 ,  C12Q1/02 ,  C12Q3/00 ,  G01N15/14 ,  C12N5/076 ,  G01N33/48 ,  G01N33/50 ,  A01N1/02 ,  G01N21/64 ,  G01N15/00 ,  G01N15/10

摘要： A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.

公开(公告)号：US20160326489A1

公开(公告)日：2016-11-10

申请号：US15179722

申请日：2016-06-10

申请人： Inguran, LLC

发明人： Gary Durack ,  Jeffrey D. Wallace ,  Gary P. Vandre ,  Lon A. Westfall ,  Jeremy T. Hatcher ,  Niraj V. Nayak

IPC分类号： C12N5/071 ,  G01N21/64 ,  G01N15/14

CPC分类号： C12N5/0612 ,  A01N1/0284 ,  C12N5/06 ,  C12N5/061 ,  C12Q1/02 ,  C12Q3/00 ,  G01N15/1468 ,  G01N15/147 ,  G01N21/63 ,  G01N21/6428 ,  G01N33/48 ,  G01N33/5005 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2021/6439 ,  Y10T436/10 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/25 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.

摘要翻译： 一种多通道系统，用于根据包括用于产生电磁辐射束的公共电磁辐射源的一种或多种特性来分类颗粒混合物中的颗粒，以及分束器，用于产生用于引导多个电磁束的多个电磁辐射束 辐射到与多通道系统的每个流动通道相关联的每个询问位置。

公开(公告)号：US09377390B2

公开(公告)日：2016-06-28

申请号：US14683936

申请日：2015-04-10

申请人： Inguran, LLC

发明人： Gary Durack ,  Jeffrey D. Wallace ,  Gary P. Vandre ,  Lon A. Westfall ,  Jeremy T. Hatcher ,  Niraj V. Nayak

IPC分类号： G01N33/48 ,  C12N5/076 ,  C12Q1/02 ,  G01N15/14 ,  C12N5/071 ,  A01N1/02 ,  C12Q3/00 ,  G01N33/50

CPC分类号： C12N5/0612 ,  A01N1/0284 ,  C12N5/06 ,  C12N5/061 ,  C12Q1/02 ,  C12Q3/00 ,  G01N15/1468 ,  G01N15/147 ,  G01N21/63 ,  G01N21/6428 ,  G01N33/48 ,  G01N33/5005 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2021/6439 ,  Y10T436/10 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/25 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.

摘要翻译： 一种多通道系统，用于根据包括用于产生电磁辐射束的公共电磁辐射源的一种或多种特性来分类颗粒混合物中的颗粒，以及分束器，用于产生用于引导多个电磁束的多个电磁辐射束 辐射到与多通道系统的每个流动通道相关联的每个询问位置。