公开(公告)号：US08907312B2

公开(公告)日：2014-12-09

申请号：US13212459

申请日：2011-08-18

申请人： Xin Heng ,  Paul Patt

发明人： Xin Heng ,  Paul Patt

IPC分类号： G01N15/06 ,  H01J3/14 ,  G01N15/14 ,  G01N21/64

CPC分类号： G01N15/1434 ,  G01N15/1436 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/645 ,  G01N2021/6421

摘要： A flow cytometry system includes a flow element through which a cell is transported in a flowing fluid. The flow element includes a bore bounded by a wall. A light source is configured to illuminate the cell. An optical system receives light emanating from the cell and directs at least some of the received light to a light sensor. The optical system includes a numerical-aperture-increasing lens at a wall of the flow element. At least some of the received light passes through the numerical-aperture-increasing lens. The flow cytometry system may also include a beam splitter that directs two wavelength bands of the emanating light such that light in two wavelength band preferentially reach different sensing locations via different paths. The system may also include an optical element placed in one of the paths, shifting a focal location of the affected path to compensate for chromatic aberration of the numerical-aperture-increasing lens.

摘要翻译： 流式细胞术系统包括流动元件，细胞在流动元件中以流动的流体输送。 流动元件包括由壁限定的孔。 光源被配置为照亮单元。 光学系统接收从单元发出的光并且将至少一些所接收的光引导到光传感器。 光学系统在流动元件的壁处包括数字孔径增加透镜。 所接收的光中的至少一些通过数字孔径增加透镜。 流式细胞术系统还可以包括分束器，其引导发射光的两个波长带，使得两个波长带中的光优先通过不同的路径到达不同的感测位置。 该系统还可以包括放置在路径之一中的光学元件，移动受影响的路径的焦点位置以补偿数值孔径增加透镜的色差。

公开(公告)号：US08609363B2

公开(公告)日：2013-12-17

申请号：US13298682

申请日：2011-11-17

申请人： Xin Heng ,  Eugene Breniman

发明人： Xin Heng ,  Eugene Breniman

IPC分类号： C12Q1/02 ,  C12Q1/00

CPC分类号： C12Q1/02 ,  G01N2015/1486

摘要： Cell counts that distinguish between live and dead cells while providing an accurate count of the total of live and dead cells are obtained by the use of a vital stain in conjunction with illumination of the cell population and the taking of light images at different wavelengths, one which is not absorbed by the stain and one that is absorbed by the stain. Masking and inaccuracies in the counting of dead cells is thereby avoided.

摘要翻译： 通过使用生命染色结合细胞群的照射和摄取不同波长的光图像获得活细胞和死细胞之间的细胞计数，同时提供活细胞和死细胞的总数的精确计数。 其不被污渍吸收并被污渍所吸收。 从而避免了死细胞计数中的掩蔽和不准确。

公开(公告)号：US08411282B2

公开(公告)日：2013-04-02

申请号：US13157245

申请日：2011-06-09

申请人： Xiquan Cui ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis ,  Xin Heng

发明人： Xiquan Cui ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis ,  Xin Heng

IPC分类号： G01B9/02

CPC分类号： G02B21/0056 ,  G01N21/553 ,  G02B21/18 ,  G02B21/361

摘要： A differential interference contrast (DIC) determination device and method utilizes an illumination source, a layer having a pair of two apertures that receive illumination from the illumination source, and a photodetector to receive Young's interference from the illumination passing through the pair of two apertures. In addition, a surface wave assisted optofluidic microscope and method utilize an illumination source, a fluid channel having a layer with at least one aperture as a surface, and a photodetector that receives a signal based on the illumination passing through the aperture. The layer is corrugated (e.g., via fabrication) and parameters of the corrugation optimize the signal received on the photodetector.

摘要翻译： 差分干涉对比度（DIC）确定装置和方法利用照明源，具有一对两个孔的层，其接收来自照明源的照明，以及光电检测器，用于接收来自穿过该对两个孔的照明的杨氏干扰。 此外，表面波辅助光流体显微镜和方法利用照明源，具有至少一个孔的层作为表面的流体通道，以及基于穿过孔的照明而接收信号的光电检测器。 该层是波纹状的（例如通过制造），并且波纹的参数优化了在光电检测器上接收的信号。

公开(公告)号：US08189204B2

公开(公告)日：2012-05-29

申请号：US12792059

申请日：2010-06-02

申请人： Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis ,  Guoan Zheng

发明人： Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis ,  Guoan Zheng

IPC分类号： G01B9/02

CPC分类号： G02B21/0056 ,  B82Y20/00 ,  G01N21/554 ,  G02B6/1226 ,  G02B21/10 ,  G02B21/14 ,  G02B27/52

摘要： Embodiments of the present invention relate to a surface wave enabled darkfield aperture structure comprising an aperture layer, a aperture in the aperture layer and a plurality of grooves around the aperture. The aperture layer has a first and second surface. The plurality of grooves is in the first surface. A surface wave propagates along at least the first surface. The plurality of grooves is configured to generate a darkfield at the aperture by modifying the surface wave to cancel out direct transmission of a uniform incident light field received by the aperture.

摘要翻译： 本发明的实施例涉及一种表面波使能暗场孔结构，其包括孔径层，孔径层中的孔和围绕孔的多个凹槽。 孔层具有第一和第二表面。 多个槽位于第一表面。 表面波沿着至少第一表面传播。 多个槽被配置为通过修改表面波来在孔处产生暗场，以消除由孔接收的均匀入射光场的直接透射。

公开(公告)号：US20110075254A1

公开(公告)日：2011-03-31

申请号：US12792059

申请日：2010-06-02

申请人： Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis ,  Guoan Zheng

发明人： Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis ,  Guoan Zheng

IPC分类号： G02B21/10 ,  G02B21/14

CPC分类号： G02B21/0056 ,  B82Y20/00 ,  G01N21/554 ,  G02B6/1226 ,  G02B21/10 ,  G02B21/14 ,  G02B27/52

摘要： Embodiments of the present invention relate to a surface wave enabled darkfield aperture structure comprising an aperture layer, a aperture in the aperture layer and a plurality of grooves around the aperture. The aperture layer has a first and second surface. The plurality of grooves is in the first surface. A surface wave propagates along at least the first surface. The plurality of grooves is configured to generate a darkfield at the aperture by modifying the surface wave to cancel out direct transmission of a uniform incident light field received by the aperture.

摘要翻译： 本发明的实施例涉及一种表面波使能暗场孔结构，其包括孔径层，孔径层中的孔和围绕孔的多个凹槽。 孔层具有第一和第二表面。 多个槽位于第一表面。 表面波沿着至少第一表面传播。 多个槽被配置为通过修改表面波来在孔处产生暗场，以消除由孔接收的均匀入射光场的直接透射。

公开(公告)号：US09068916B2

公开(公告)日：2015-06-30

申请号：US13039990

申请日：2011-03-03

申请人： Xin Heng

发明人： Xin Heng

IPC分类号： G06K9/00 ,  G01N15/14 ,  G01N21/64

CPC分类号： G01N15/147 ,  G01N15/1475 ,  G01N15/1484 ,  G01N21/6428 ,  G01N2015/149

摘要： A microassembled imaging cytometer includes a sensing location that undergoes relative motion with a cell. Light from a light source is focused by a focusing element to a plurality of focused illumination spots or lines at the sensing location, illuminating the cell as the cell traverses the sensing location. A collection lens collects light emanating from the cell and refocuses the collected light onto an array light sensor. The focusing element may include an array of microlenses having spherical or aspheric surfaces. The system may include a processing unit that constructs a digital image of the cell based at least in part on signals produced by the array light sensor indicating the intensity and distribution of light falling on the array light sensor. The system may characterize cells using light emanating from the cells by fluorescence.

摘要翻译： 微组装成像细胞计数器包括与细胞经历相对运动的感测位置。 来自光源的光通过聚焦元件聚焦到感测位置处的多个聚焦照明点或线，当小区穿过感测位置时照亮该小区。 收集透镜收集从细胞发出的光并将收集的光重新聚焦到阵列光传感器上。 聚焦元件可以包括具有球面或非球面的微透镜阵列。 系统可以包括处理单元，其至少部分地基于由阵列光传感器产生的指示落在阵列光传感器上的光的强度和分布的信号构成单元的数字图像。 该系统可以使用通过荧光从细胞发出的光来表征细胞。

公开(公告)号：US09041938B2

公开(公告)日：2015-05-26

申请号：US13344550

申请日：2012-01-05

申请人： Guoan Zheng ,  Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer

发明人： Guoan Zheng ,  Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer

IPC分类号： G01B9/02 ,  G02B6/122 ,  B82Y20/00 ,  G01N21/552 ,  G02B5/00 ,  G02B21/00 ,  G02B21/14 ,  G02B27/52

CPC分类号： G02B6/1226 ,  B82Y20/00 ,  G01N21/554 ,  G02B5/008 ,  G02B21/0032 ,  G02B21/004 ,  G02B21/0056 ,  G02B21/0068 ,  G02B21/14 ,  G02B27/52

摘要： A surface wave assisted system having an aperture layer with a surface and an aperture, and a plurality of grooves around the aperture. The plurality of grooves is configured to generate an optical transfer function at the aperture by inducing a surface wave for interfering with transmission of light of a range of spatial frequency.

摘要翻译： 表面波辅助系统，具有具有表面和孔径的孔层，以及围绕孔的多个凹槽。 多个槽被配置为通过诱导表面波来干扰空间频率范围的光的透射来在孔处产生光学传递函数。

公开(公告)号：US08314933B2

公开(公告)日：2012-11-20

申请号：US12398050

申请日：2009-03-04

申请人： Xiquan Cui ,  Xin Heng ,  Lap Man Lee ,  Changhuei Yang

发明人： Xiquan Cui ,  Xin Heng ,  Lap Man Lee ,  Changhuei Yang

IPC分类号： G01N21/00

CPC分类号： G01N21/53 ,  G01N21/6458

摘要： Embodiments of the present invention relate to techniques for improving optofluidic microscope (OFM) devices. One technique which may be used eliminates the aperture layer covering the light detector layer. Other techniques retain the aperture layer, reversing the relative position of the light source and light detector such that light passes through the aperture layer before passing through the fluid channel to the light detector. Another technique adds an optical tweezer for controlling the movement of objects moving through the fluid channel. Another technique adds an optical fiber bundle to relay light from light transmissive regions to a remote light detector. Another technique adds two electrodes at ends of the fluid channel to generate an electrical field capable of moving objects through the fluid channel while suppressing rotation. These techniques can be employed separately or in combination to improve the capabilities of OFM devices.

摘要翻译： 本发明的实施例涉及用于改进光流体显微镜（OFM）装置的技术。 可以使用的一种技术消除了覆盖光检测器层的孔径层。 其他技术保留孔径层，反转光源和光检测器的相对位置，使得光在通过流体通道之前通过孔层，到达光检测器。 另一种技术增加了用于控制移动通过流体通道的物体运动的光学镊子。 另一种技术增加了光纤束，以将来自光透射区域的光中继到远程光检测器。 另一种技术在流体通道的端部添加两个电极，以产生能够通过流体通道移动物体同时抑制旋转的电场。 这些技术可以单独使用或组合使用以提高OFM设备的能力。

公开(公告)号：US20120026509A1

公开(公告)日：2012-02-02

申请号：US13157245

申请日：2011-06-09

申请人： Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis

发明人： Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis

IPC分类号： G01B9/02 ,  G02B21/10 ,  G02B5/18

CPC分类号： G02B21/0056 ,  G01N21/553 ,  G02B21/18 ,  G02B21/361

摘要： A differential interference contrast (DIC) determination device and method utilizes an illumination source, a layer having a pair of two apertures that receive illumination from the illumination source, and a photodetector to receive Young's interference from the illumination passing through the pair of two apertures. In addition, a surface wave assisted optofluidic microscope and method utilize an illumination source, a fluid channel having a layer with at least one aperture as a surface, and a photodetector that receives a signal based on the illumination passing through the aperture. The layer is corrugated (e.g., via fabrication) and parameters of the corrugation optimize the signal received on the photodetector.

摘要翻译： 差分干涉对比度（DIC）确定装置和方法利用照明源，具有一对两个孔的层，其接收来自照明源的照明，以及光电检测器，用于接收来自穿过该对两个孔的照明的杨氏干扰。 此外，表面波辅助光流体显微镜和方法利用照明源，具有至少一个孔的层作为表面的流体通道，以及基于穿过孔的照明而接收信号的光电检测器。 该层是波纹状的（例如通过制造），并且波纹的参数优化了在光电检测器上接收的信号。

公开(公告)号：US20120295300A1

公开(公告)日：2012-11-22

申请号：US13298682

申请日：2011-11-17

申请人： Xin Heng ,  Eugene Breniman

发明人： Xin Heng ,  Eugene Breniman

IPC分类号： G01N21/17

CPC分类号： C12Q1/02 ,  G01N2015/1486

摘要： Cell counts that distinguish between live and dead cells while providing an accurate count of the total of live and dead cells are obtained by the use of a vital stain in conjunction with illumination of the cell population and the taking of light images at different wavelengths, one which is not absorbed by the stain and one that is absorbed by the stain. Masking and inaccuracies in the counting of dead cells is thereby avoided.

摘要翻译： 通过使用生命染色结合细胞群的照射和摄取不同波长的光图像获得活细胞和死细胞之间的细胞计数，同时提供活细胞和死细胞的总数的精确计数。 其不被污渍吸收并被污渍所吸收。 从而避免了死细胞计数中的掩蔽和不准确。