公开(公告)号：US12066617B2

公开(公告)日：2024-08-20

申请号：US17226734

申请日：2021-04-09

申请人： UCHICAGO ARGONNE, LLC

发明人： Gary P. Wiederrecht ,  Stephen K. Gray ,  Xiewen Wen ,  Sushovit Adhikari ,  Cristian Leonardo Cortes ,  David J. Gosztola

IPC分类号： G02B21/18 ,  G02F1/37 ,  G06T5/50 ,  G01J3/28

CPC分类号： G02B21/18 ,  G02F1/37 ,  G06T5/50 ,  G01J3/2823 ,  G06T2207/10056

摘要： A system and methods for ghost imaging second harmonic generation microscopy. Imaging data is collected in parallel, providing faster imagine reconstruction and enabling reconstruction in scattering environments. Ghost imaging, split light beam interacting with a target and a second light beam unimpeded and not required to pass through the same background. A second harmonic generation image is reconstructed from the detected photons.

公开(公告)号：US12038630B1

公开(公告)日：2024-07-16

申请号：US18102747

申请日：2023-01-29

申请人： Designs for Vision, Inc.

发明人： Richard E. Feinbloom ,  Moty Solomon

IPC分类号： G02C9/04 ,  G02B21/18 ,  G02B21/36 ,  G02B23/08 ,  G02B23/14 ,  G02B23/16 ,  G02B25/00 ,  G02B27/14 ,  A61B90/00 ,  G02B5/20 ,  H04N7/18

CPC分类号： G02C9/04 ,  G02B21/18 ,  G02B21/361 ,  G02B21/362 ,  G02B23/08 ,  G02B23/14 ,  G02B23/16 ,  G02B25/004 ,  G02B25/007 ,  G02B25/008 ,  G02B27/144 ,  A61B90/361 ,  G02B5/20 ,  G02B25/001 ,  H04N7/183

摘要： A telescopic/image capture device that allows for the concurrent viewing and capturing an image of an object, wherein the captured image is magnified to the same level than that of the viewed image. Further included are filters incorporated into the device to allow for the attenuation of light in undesired wavelengths while allowing light in desired wavelengths to pass unattenuated. In still a further aspect of the invention, a second telescopic lens is incorporated into the path of light to be captured by the image capture device, wherein the image captured and recorded is of a greater magnification than that of the user viewable image.

公开(公告)号：US20240210671A1

公开(公告)日：2024-06-27

申请号：US18254448

申请日：2021-12-13

申请人： Washington University

发明人： Matthew Lew ,  Oumeng Zhang

IPC分类号： G02B21/36 ,  G02B21/18 ,  G02B27/14 ,  G02B27/28

CPC分类号： G02B21/361 ,  G02B21/18 ,  G02B27/14 ,  G02B27/283 ,  G02B27/286

摘要： An imaging system uses a multi-view reflector (MVR) to simultaneously measure the three-dimensional (3D) position and orientation of a light emitter. The MVR is positioned at a back focal plane (BFP) of a light collecting optical system, and uses pyramid reflectors and/or conical reflectors to reflect light in at least two regions of the BFP to at least two detection channels of a detector. The at least two detection channels produce images that are used to determine the 3D position and orientation of a light emitter. The system may be used, for example, to image the structure of molecules or organelles in a cell, track the movement of molecules in a cell, or to study the interaction of molecules within a cell.

公开(公告)号：US20230393409A1

公开(公告)日：2023-12-07

申请号：US18249981

申请日：2021-10-15

申请人： Leica Instruments (Singapore) Pte. Ltd.

发明人： Gao YANG

IPC分类号： G02B27/14 ,  G02B21/18

CPC分类号： G02B27/144 ,  G02B21/18

摘要： An optical beam splitter including an at least partially semi-transparent layer. The at least partially semi-transparent layer includes at least one first region having a first transmission coefficient and a first reflection coefficient for visible light and at least one second region having a second transmission coefficient for visible light. The first transmission coefficient is larger than 0 and the first reflection coefficient is larger than 0. The second transmission coefficient differs from the first transmission coefficient. The optical beam splitter is configured so that visible light passing the at least partially semi-transparent layer through the at least one first region propagates along a first optical output path of the optical beam splitter and visible light reflected by the at least one first region propagates along a second optical output path of the optical beam splitter.

公开(公告)号：US11808707B2

公开(公告)日：2023-11-07

申请号：US16854213

申请日：2020-04-21

申请人： THERMO ELECTRON SCIENTIFIC INSTRUMENTS LLC

发明人： George L. Skupniewicz ,  Francis J. Deck

IPC分类号： G01N21/65 ,  G02B27/10 ,  G02B26/08 ,  G02B21/00 ,  G02B21/18 ,  G02B27/14 ,  G02B21/36 ,  G01N21/64 ,  G02B21/16

CPC分类号： G01N21/65 ,  G01N21/64 ,  G02B21/0004 ,  G02B21/16 ,  G02B21/18 ,  G02B21/362 ,  G02B26/0816 ,  G02B27/1006 ,  G02B27/141 ,  G01N2201/0636 ,  G01N2201/06113 ,  G01N2201/084

摘要： An embodiment of a module system configured to interface with a microscope is described that comprises an input optical fiber configured to provide an excitation light beam from an external light source; dynamic alignment mirrors configured to adjust the position of the beams paths of the excitation light beam on a first plane; a coupling comprising a first end configured to engage with a complementary end, wherein the excitation light reflects off a turning mirror and travels along a beam path on a second plane through an orifice in the coupling; and an output optical fiber for delivering light from a sample to an external detector, wherein the light from the sample travels along the beam path on the second plane through the orifice in the coupling, reflects off the turning mirror and travels along one of the beam paths on the first plane to the output optical fiber.

公开(公告)号：US20230213748A1

公开(公告)日：2023-07-06

申请号：US18008442

申请日：2021-11-15

申请人： MACHINE VISION LIGHTING INC.

发明人： Shigeki MASUMURA

IPC分类号： G02B21/18

CPC分类号： G02B21/18

摘要： To make an object to be observed observable with high resolution and to make an inclination angle of a surface of the object to be observed recognizable over a wide range.
An image observation system 100 provided with a lighting optical system 116 irradiating an object to be observed W with an illumination light and an observation optical system 122 collecting object light from the object to be observed W to guide to a detector 126, the image observation system 100 comprising an objective lens 122A opposed to the object to be observed W, a beam splitter 116B disposed on an opposite side to the object to be observed W with respect to the objective lens 122A, and a relay image RI of the illumination light splitting member 114 for dividing wavelength regions R, G and B of the illumination light into a plurality of different solid angle regions IS1, IS2 and IS3, being disposed in front of the objective lens 122A.

公开(公告)号：US11674897B2

公开(公告)日：2023-06-13

申请号：US17698215

申请日：2022-03-18

申请人： Purdue Research Foundation

发明人： Delong Zhang ,  Ji-Xin Cheng

IPC分类号： G01N21/3563 ,  G01N21/17 ,  C12Q1/02 ,  G01J5/22 ,  G01J5/00 ,  G02B21/02 ,  G02B21/06 ,  G02B21/18 ,  H01S5/34

CPC分类号： G01N21/3563 ,  C12Q1/02 ,  G01J5/22 ,  G01N21/171 ,  G01J2005/0077 ,  G01N2021/1712 ,  G01N2201/0612 ,  G01N2201/0697 ,  G02B21/02 ,  G02B21/06 ,  G02B21/18 ,  H01S5/3401

摘要： Systems and methods for sensing vibrational absorption induced photothermal effect via a visible light source. A Mid-infrared photothermal probe (MI-PTP, or MIP) approach achieves 10 mM detection sensitivity and sub-micron lateral spatial resolution. Such performance exceeds the diffraction limit of infrared microscopy and allows label-free three-dimensional chemical imaging of live cells and organisms. Distributions of endogenous lipid and exogenous drug inside single cells can be visualized. MIP imaging technology may enable applications from monitoring metabolic activities to high-resolution mapping of drug molecules in living systems, which are beyond the reach of current infrared microscopy.

公开(公告)号：US20190011365A1

公开(公告)日：2019-01-10

申请号：US15752417

申请日：2016-09-06

申请人： DIRECT GENOMICS CO., LTD.

发明人： Liangjin Ge ,  Luyang Zhao ,  Ping Wu ,  Qin Yan

IPC分类号： G01N21/64 ,  G02B21/18 ,  G02B21/16 ,  G02B21/06

CPC分类号： G01N21/6452 ,  G01N21/6458 ,  G01N21/648 ,  G01N2021/6419 ,  G02B21/0076 ,  G02B21/06 ,  G02B21/16 ,  G02B21/18 ,  G02B21/245 ,  G02B27/141

摘要： The present disclosure discloses a total internal reflection fluorescence imaging system and a sequencing device. The total internal reflection fluorescence imaging system includes a first imaging system and a second imaging system. The first imaging system includes N separate laser light paths. The second imaging system determines the changes of the height of the sample by means of the differences of the positions at which the second receiving device acquires the second images, and performs automatic compensation according to the changes of the height of the sample, so as to ensure that the sample is always at a focused position. The embodiment can perform light path adjustment of the N separate laser light paths respectively by means of the second imaging system, so as to realize that the N separate laser light paths have the same penetration depth. Therefore no further light path adjustment is required when switching between different light sources, thus saving the time consuming for light path adjustment.

公开(公告)号：US20180259763A1

公开(公告)日：2018-09-13

申请号：US15977280

申请日：2018-05-11

申请人： OLYMPUS CORPORATION

发明人： Hiroshi ISHIWATA

IPC分类号： G02B21/36 ,  G02B21/18 ,  H04N13/207 ,  H04N13/106 ,  G02B7/34 ,  H04N13/00

CPC分类号： G02B21/367 ,  G02B7/34 ,  G02B21/18 ,  H04N13/106 ,  H04N13/207 ,  H04N13/211 ,  H04N13/218 ,  H04N13/254 ,  H04N2013/0081 ,  H04N2013/0092

摘要： A three-dimensional position information acquiring method includes acquiring an image of a first optical image; thereafter acquiring an image of a second optical image; and performing a computation using image data of the first and second optical images. Acquisition of the image of the first optical image is based on light beams having passed through a first area. Acquisition of the image of the second optical image is based on light beams having passed through a second area. The positions of the centers of the first and second areas are both away from the optical axis of an optical system in a plane perpendicular to said optical axis. The first and second areas respectively include at least portions that do not overlap with each other. Three-dimensional position information about an observed object is acquired by the computation. The first and second areas are formed at rotationally symmetric positions. The computation performs subtraction processing using data of the first and second images, generates a subtracted image and then determined whether the observed object is displaced on the front or rear side relative to an in-focus position.

公开(公告)号：US10073259B2

公开(公告)日：2018-09-11

申请号：US15220100

申请日：2016-07-26

申请人： Ventana Medical Systems, Inc.

发明人： Karl Garsha ,  Michael Otter

IPC分类号： G02B21/36 ,  G01N21/64 ,  G02B21/00 ,  G02B21/18

CPC分类号： G02B21/367 ,  G01N21/64 ,  G01N21/6458 ,  G02B21/00 ,  G02B21/18 ,  G02B21/361 ,  G02B2207/113

摘要： A microscope-based system and method for simultaneous imaging of several object planes, of a three-dimensional (3D) sample, associated with different depths throughout the sample. The system includes a polyfocal optical portion, adapted to create a plurality of optical channels each of which is associated with an image of a corresponding object plane, and a spectrally-selective portion, adapted to transform the spectral distribution of the image-forming beam of light to a corresponding spatial distribution. The image, registered by a detector, includes an image of an object plane and an image of the spatially-coded spectral distribution. The method effectuates the simultaneous multispectral imaging of the several object planes. The required data-acquisition time is several fold shorter than that taken by a conventional multispectral microscope-based imaging system.