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公开(公告)号：US20160334613A1
公开(公告)日：2016-11-17
申请号：US15149855
申请日：2016-05-09
Applicant: OLYMPUS CORPORATION
Inventor： Hiroshi ISHIWATA
CPC classification number: G02B21/361 , G02B21/0024 , G02B21/0032 , G02B21/22 , G02B21/367
Abstract: A three-dimensional information obtaining apparatus includes an illumination optical system that illuminates a sample with a light sheet, an imaging device that has a two-dimensional imaging element and that captures an image of the sample illuminated by the illumination optical system, an observation optical system that forms, on the two-dimensional imaging element, a plurality of optical images of the sample observed from a plurality of different directions, and an arithmetic device that calculates three-dimensional information of the sample from a plurality of pieces of image data of the sample. A thickness D of the light sheet satisfies D≦8·PP/β/NA when PP>λ·β/(4·NA) is satisfied, where PP is a pixel pitch of the two-dimensional imaging element, λ is a wavelength of observation light, β is a magnification of the plurality of optical images, and NA is a numerical aperture on an object side of the observation optical system.
Abstract translation: 一种三维信息获取装置包括：用光照射样品的照明光学系统，具有二维成像元件并且捕获由照明光学系统照射的样本的图像的成像装置，观察光学 在二维成像元件上形成从多个不同方向观察到的样本的多个光学图像的运算装置，以及从多个图像数据的多个图像数据计算样本的三维信息的运算装置， 例子。 当满足PP>λ·β/（4·NA）时，光片的厚度D满足D≤8·PP /β/ NA，其中PP是二维成像元件的像素间距，λ是波长 的观察光，β是多个光学图像的倍率，NA是观察光学系统的物体侧的数值孔径。
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2.发明申请公开(公告)号：US20160171679A1
公开(公告)日：2016-06-16
申请号：US14970924
申请日：2015-12-16
Applicant: OLYMPUS CORPORATION
Inventor： Hiroshi ISHIWATA
CPC classification number: G02B21/367 , G02B7/34 , G02B21/18 , H04N13/106 , H04N13/207 , H04N13/211 , H04N13/218 , H04N13/254 , H04N2013/0081 , H04N2013/0092
Abstract: A three-dimensional position information acquiring method includes acquiring a first image of a first optical image, acquiring a second image of a second optical image, and performing a predetermined computation using data of the first image and data of the second image, wherein acquisition of the first image is performed based on light beams having passed through a first area, acquisition of the second image is performed based on light beams having passed through a second area, the position of the center of the first area and the position of the center of the second area are both away from the optical axis in a plane perpendicular to the optical axis, the first area and the second area respectively include at least portions that do not overlap with each other, and three dimensional position information about an observed object is acquired by the predetermined computation.
Abstract translation: 三维位置信息获取方法包括获取第一光学图像的第一图像，获取第二光学图像的第二图像，以及使用第一图像的数据和第二图像的数据执行预定的计算，其中获取 基于通过第一区域的光束执行第一图像，基于通过第二区域的光束，第一区域的中心位置和中心位置的位置来执行第二图像的获取 第二区域在与光轴垂直的平面中都远离光轴，第一区域和第二区域分别至少包括彼此不重叠的部分，并且获取关于被观察物体的三维位置信息 通过预定的计算。
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公开(公告)号：US20150185460A1
公开(公告)日：2015-07-02
申请号：US14571244
申请日：2014-12-15
Applicant: OLYMPUS CORPORATION
Inventor： Eiji NAKASHO , Hiroshi ISHIWATA
CPC classification number: G02B21/16 , G02B21/0056 , G02B21/008
Abstract: An image forming method for a biological sample includes calculating a component corresponding to a phase distribution of the sample and a component corresponding to a matter other than the phase distribution according to a plurality of pieces of images with different image contrasts, to form a normalized phase component; separating the phase component image into a plurality of frequency components according to spatial frequencies of the image; merging the phase distribution of the refraction component and the phase distribution of the structure component calculated by applying a deconvolution process to each of the frequency components using an optical response characteristic corresponding to each, to calculate the phase distribution, and forming a phase distribution image from the calculated phase distribution; and merging the phase distribution image with an image of the sample in which a biochemical phenomenon and/or a physical phenomenon in the sample are visualized.
Abstract translation: 用于生物样品的图像形成方法包括根据具有不同图像对比度的多个图像计算与样本的相位分布相对应的分量和与相位分布以外的事物相对应的分量，以形成标准化相位 零件; 根据图像的空间频率将相位分量图像分离成多个频率分量; 通过使用对应于每个频率分量的光响应特性来合并折射分量的相位分布和通过对每个频率分量应用去卷积处理而计算的结构分量的相位分布，以计算相位分布，并且从 计算相位分布; 并将相位分布图像与样品中的生物化学现象和/或物理现象可视化的图像合并。
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4.发明申请公开(公告)号：US20180002670A1
公开(公告)日：2018-01-04
申请号：US15631831
申请日：2017-06-23
Applicant: OLYMPUS CORPORATION
Inventor： Hiroshi ISHIWATA
CPC classification number: C12N5/0696 , G01N15/14 , G01N15/1434 , G01N15/1475 , G01N33/4833 , G01N2015/1006 , G01N2015/1454 , G06T7/00 , G06T7/0012 , G06T7/11 , G06T7/168 , G06T7/50 , G06T2207/10056 , G06T2207/30004 , G06T2207/30024
Abstract: An operation device includes: a communication unit that receives signals of images of a biological sample and outputs data to a display medium, the images being captured by a microscope that converts a phase distribution into an image intensity distribution; and a calculator that calculates a first phase distribution of the biological sample from the image signals. The calculator extracts a region having a phase amount that is not less than a specified phase amount from the first phase distribution, and generates evaluation information by using the region having a phase amount that is not less than the specified phase amount, that is an indicator used when a user evaluates a state of the biological sample; and the communication unit outputs the evaluation information to the display medium.
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Patent Agency Ranking
公开(公告)号：US20160187635A1
公开(公告)日：2016-06-30
申请号：US14980002
申请日：2015-12-28
Applicant: OLYMPUS CORPORATION
Inventor： Bunryu ARASHI , Hiroshi ISHIWATA
CPC classification number: G02B21/14 , G02B21/0032 , G02B21/06
Abstract: A phase contrast microscope includes a light source, an illumination optical system configured to irradiate an observation object with the light from the light source, and a magnifying optical system configured to magnify and project the observation object, the illumination optical system includes a ring slit, the magnifying optical system includes an objective provided with a phase film, the ring slit is disposed at a pupil position of the illumination optical system, the phase film is disposed at a position conjugate to the pupil position of the illumination optical system, and the following conditional expression (1) is satisfied: r/I≦0.25 (1) where r is a central part radius of the phase film, and I is a radius of a pupil of the objective.
Abstract translation: 相位显微镜包括光源，被配置为用来对来自光源的光照射观察对象物的照明光学系统，以及放大和投影观察对象物的放大光学系统，所述照明光学系统包括环形狭缝， 放大光学系统包括设置有相膜的物镜，环形狭缝设置在照明光学系统的光瞳位置，相位膜设置在与照明光学系统的光瞳位置共轭的位置，并且以下 条件表达式（1）满足：r / I＆nlE; 0.25（1）其中r是相膜的中心部分半径，I是物镜的光瞳的半径。
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6.发明申请公开(公告)号：US20140285650A1
公开(公告)日：2014-09-25
申请号：US14189532
申请日：2014-02-25
Applicant: OLYMPUS CORPORATION
Inventor： Hiroshi ISHIWATA
CPC classification number: G06K9/00127 , G01N15/1475 , G02B21/0056 , G02B21/0068 , G02B21/14 , G02B21/16 , G02B21/365 , G06K9/0014
Abstract: A phase distribution measurement method inside a biological sample includes taking in an optical image of the sample formed by a microscope to form a plurality of images with different image contrasts; calculating a component corresponding to phase distribution of the sample and a component corresponding to other than the phase distribution, and dividing the component corresponding to the phase distribution by the component corresponding to other than the phase distribution to forma normalized phase component image; breaking down the phase component image into a plurality of frequency components; performing a deconvolution process to each of the frequency components using an optical response character corresponding to each, and calculating phase distribution of a refraction component and phase distribution of a structure component; and calculating phase distribution of the sample by compounding the phase distribution of the refraction component and the phase distribution of the structure component.
Abstract translation: 生物样品内部的相位分布测量方法包括摄取由显微镜形成的样品的光学图像，以形成具有不同图像对比度的多个图像; 计算与样本的相位分布对应的分量和与相位分布不同的分量，将与相位分布对应的分量除以与相位分布对应的分量，形成归一化相位分量图像; 将相位分量图像分解为多个频率分量; 对每个频率分量使用与每个频率分量对应的光响应特性进行去卷积处理，并计算折射分量的相位分布和结构分量的相位分布; 以及通过将折射分量的相位分布和结构分量的相位分布相组合来计算样本的相位分布。
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7.发明申请公开(公告)号：US20180259763A1
公开(公告)日：2018-09-13
申请号：US15977280
申请日：2018-05-11
Applicant: OLYMPUS CORPORATION
Inventor： Hiroshi ISHIWATA
IPC: G02B21/36 , G02B21/18 , H04N13/207 , H04N13/106 , G02B7/34 , H04N13/00
CPC classification number: G02B21/367 , G02B7/34 , G02B21/18 , H04N13/106 , H04N13/207 , H04N13/211 , H04N13/218 , H04N13/254 , H04N2013/0081 , H04N2013/0092
Abstract: A three-dimensional position information acquiring method includes acquiring an image of a first optical image; thereafter acquiring an image of a second optical image; and performing a computation using image data of the first and second optical images. Acquisition of the image of the first optical image is based on light beams having passed through a first area. Acquisition of the image of the second optical image is based on light beams having passed through a second area. The positions of the centers of the first and second areas are both away from the optical axis of an optical system in a plane perpendicular to said optical axis. The first and second areas respectively include at least portions that do not overlap with each other. Three-dimensional position information about an observed object is acquired by the computation. The first and second areas are formed at rotationally symmetric positions. The computation performs subtraction processing using data of the first and second images, generates a subtracted image and then determined whether the observed object is displaced on the front or rear side relative to an in-focus position.
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公开(公告)号：US20180217369A1
公开(公告)日：2018-08-02
申请号：US15863153
申请日：2018-01-05
Applicant: OLYMPUS CORPORATION
Inventor： Hiroshi ISHIWATA
Abstract: A phase distribution calculation method includes: moving a focal position of an optical system to a plurality of positions within a phase object, the plurality of positions being different from each other in an optical axis direction of the optical system, and acquiring an image via the optical system in each of the plurality of positions; calculating a first phase distribution of a three-dimensional region that corresponds to a plurality of images acquired in the plurality of positions, in accordance with the plurality of images; and correcting the first phase distribution in accordance with a change in the optical axis direction in a region of interest specified in the three-dimensional region.
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公开(公告)号：US20170351083A1
公开(公告)日：2017-12-07
申请号：US15607223
申请日：2017-05-26
Applicant: OLYMPUS CORPORATION
Inventor： Hiroshi ISHIWATA
CPC classification number: G02B21/367 , G02B21/0088 , G02B21/14 , G02B21/361 , G02B27/50 , G02B27/52
Abstract: A phase object visualization apparatus includes: an illumination optical system 11 that illuminates a phase object; an image formation optical system 12 that forms an image from light from sample S that corresponds to the phase object; and light blocking unit 10 for blocking light, the light blocking unit 10 being disposed between the sample S and an image plane formed by the image formation optical system 12, and including an aperture at a position decentered from the optical axis of the image formation optical system 12. The position of the aperture is such that an area occupied on the aperture by 0-order diffraction light from the sample S illuminated by the illumination optical system 11 becomes smaller than the total area of the aperture.
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