公开(公告)号：US20150185460A1

公开(公告)日：2015-07-02

申请号：US14571244

申请日：2014-12-15

Applicant: OLYMPUS CORPORATION

Inventor： Eiji NAKASHO ,  Hiroshi ISHIWATA

IPC: G02B21/16 ,  G02B21/00

CPC classification number: G02B21/16 ,  G02B21/0056 ,  G02B21/008

Abstract: An image forming method for a biological sample includes calculating a component corresponding to a phase distribution of the sample and a component corresponding to a matter other than the phase distribution according to a plurality of pieces of images with different image contrasts, to form a normalized phase component; separating the phase component image into a plurality of frequency components according to spatial frequencies of the image; merging the phase distribution of the refraction component and the phase distribution of the structure component calculated by applying a deconvolution process to each of the frequency components using an optical response characteristic corresponding to each, to calculate the phase distribution, and forming a phase distribution image from the calculated phase distribution; and merging the phase distribution image with an image of the sample in which a biochemical phenomenon and/or a physical phenomenon in the sample are visualized.

Abstract translation: 用于生物样品的图像形成方法包括根据具有不同图像对比度的多个图像计算与样本的相位分布相对应的分量和与相位分布以外的事物相对应的分量，以形成标准化相位 零件; 根据图像的空间频率将相位分量图像分离成多个频率分量; 通过使用对应于每个频率分量的光响应特性来合并折射分量的相位分布和通过对每个频率分量应用去卷积处理而计算的结构分量的相位分布，以计算相位分布，并且从 计算相位分布; 并将相位分布图像与样品中的生物化学现象和/或物理现象可视化的图像合并。

公开(公告)号：US20190278070A1

公开(公告)日：2019-09-12

申请号：US16292487

申请日：2019-03-05

Applicant: OLYMPUS CORPORATION

Inventor： Eiji NAKASHO ,  Hiroshi SASAKI

IPC: G02B21/00 ,  G02B21/08 ,  G02B21/36 ,  G02B5/20 ,  G02B5/00 ,  H04N5/225

Abstract: Provided are an apparatus and a method that can acquire a shaded three-dimensional image having a high contrast for a thick cell culture specimen. Provided are an apparatus and a method that observe a biological sample accommodated in a container by a modulation contrast method using a near-infrared wavelength, at least the bottom surface of the container being formed of a plastic raw material.

公开(公告)号：US20140063599A1

公开(公告)日：2014-03-06

申请号：US13893084

申请日：2013-05-13

Applicant: OLYMPUS CORPORATION

Inventor： Eiji NAKASHO

IPC: G01N21/01

CPC classification number: G01N21/01 ,  G02B21/33

Abstract: An immersion liquid retainer is an immersion liquid retainer used for an observation made with a microscope including an immersion objective. The immersion liquid retainer includes a fixing part in which a first penetration hole for viewing an observed portion of a specimen is formed and which is fixed to the specimen, and an immersion liquid retaining part where a concave part that has a bottom surface configured with a transparent flat plate and is intended to retain an immersion liquid is formed. The immersion liquid retaining part is joined to the fixing part or formed integrally with the fixing part so that the transparent flat plate covers the first penetration hole.

Abstract translation: 浸没液体保持器是用于使用具有浸没物镜的显微镜进行的观察的浸液保持器。 浸渍液保持器包括：固定部，在该固定部中形成有用于观察被检体的观察部的第一贯通孔，并固定在试样上;浸渍液保持部，其中，具有底面的凹部 透明平板，旨在保持形成浸液。 浸渍液保持部与固定部接合或与固定部一体形成，透明平板覆盖第一贯通孔。