公开(公告)号：US20210325401A1

公开(公告)日：2021-10-21

申请号：US16932445

申请日：2020-07-17

Applicant: ProteinSimple

Inventor： Irina Georgievna KAZAKOVA ,  Crystal TRAN ,  Jessica DERMODY ,  Annegret BOGE ,  Tom YANG

IPC: G01N33/68 ,  G01N33/53

Abstract: Some embodiments described herein relate to systems and methods operable to combine immunoassay and Total Protein techniques in a single sample run. Some embodiments described herein allow for multiple sequential immunoassays to be performed in the same microfluidic device. Some embodiments described herein relate to stripping reagents operable to remove primary antibodies associated with immunoassays. Such stripping reagents can allow for additional immunoassays and/or Total Protein assays to be performed on the same sample.

公开(公告)号：US10746733B2

公开(公告)日：2020-08-18

申请号：US16296618

申请日：2019-03-08

Applicant: ProteinSimple

Inventor： Charles Stanwood ,  Noel Daigneault ,  Jeffrey Branciforte

IPC: B01L3/00 ,  G05D7/00 ,  C12M1/40 ,  F16K31/00 ,  G01N33/543 ,  G01N33/53

Abstract: A microfluidic device for conducting a fluid assay includes an injection-molded (or “molded”) fluidics layer having at least one microfluidic channel configured to allow assay fluids to flow there-along, the channel having channel side walls, a channel bottom, and a channel 3D geometry, and the fluidics layer being made from injection-molded liquid silicone (or PDMS). Having the fluidics layer made from injection molded liquid silicone enables smaller-sized channel features, such as microfluidic valves and pistons, smaller channel dimensions and spacing (providing smaller device footprint, higher device capacity and other benefits), and various geometries for the channels and channel features.

公开(公告)号：US09766206B2

公开(公告)日：2017-09-19

申请号：US14039995

申请日：2013-09-27

Applicant: ProteinSimple

Inventor： Tom W. Yang ,  David John Roach

IPC: G01N27/447 ,  C07K1/28 ,  C07K1/26

CPC classification number: G01N27/44782 ,  C07K1/26 ,  C07K1/285 ,  G01N27/44704 ,  G01N27/44726 ,  G01N27/44791

Abstract: An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided.

公开(公告)号：US20160370319A1

公开(公告)日：2016-12-22

申请号：US15160636

申请日：2016-05-20

Applicant: ProteinSimple

Inventor： Joshua I. Molho ,  Kelly Gardner ,  Danh C. Tran ,  Eric Jabart ,  David Nilson ,  Yuri Kouchnir ,  Michael Huston ,  Colin B. Kennedy ,  David John Roach

IPC: G01N27/447

CPC classification number: G01N27/44782 ,  G01N27/44704 ,  G01N27/44726 ,  G01N27/44739 ,  G01N27/44752 ,  G01N27/44756

Abstract: A system for assaying a biological sample for a presence of a target analyte includes an assaying device and a computer controller. The assaying device includes a housing, a receptacle disposed in the housing, and a source of activation energy. The receptacle is configured to accept an electrophoresis cell. The electrophoresis cell has a recess area configured to accept a chip configured to accept the biological sample. The chip includes a polymeric separation medium with activatable functional groups that covalently bond to the target analyte when activated. The source of activation energy is configured to supply activation energy to activate the activatable functional groups. The computer controller is operably coupled to the source of activation energy and is configured to activate the source of activation energy to direct an application of activation energy to the polymeric separation medium to activate the activatable functional groups.

Abstract translation: 用于测定生物样品以存在目标分析物的系统包括测定装置和计算机控制器。 测定装置包括壳体，设置在壳体中的容器和激活能源。 容器被配置为接受电泳池。 电泳槽具有凹陷区域，其被配置为接受构造成接受生物样品的芯片。 该芯片包括具有可激活官能团的聚合物分离介质，其在被激活时共价键合到目标分析物。 活化能源被配置为提供活化能以激活可激活的官能团。 计算机控制器可操作地耦合到激活能源，并且被配置为激活激活能量源以将激活能量的施加引导到聚合物分离介质以激活可激活的官能团。

公开(公告)号：US20150070699A1

公开(公告)日：2015-03-12

申请号：US14222776

申请日：2014-03-24

Applicant: ProteinSimple

Inventor： Frederick David King ,  Clark Adrien Merchant ,  Peter Oma

IPC: G01N15/02

CPC classification number: G01N15/0227 ,  G01N15/0211 ,  G01N15/1463 ,  G01N21/51 ,  G01N21/53 ,  G01N21/64 ,  G01N2015/1486 ,  G01N2015/1493

Abstract: A method and a particle analyzer are provided for determining a particle size distribution of a liquid sample including particles of a lower size range, particles of an intermediate size range, and particles of an upper size range. A dark-field image frame is captured in which the particles of the lower size range and the particles of the intermediate size range are resolved, and a bright-field image frame is captured in which the particles of the intermediate size range and the particles of the upper size range are resolved. Absolute sizes of the particles of the intermediate size range and the particles of the upper size range are determined from the bright-field image frame. Calibrated sizes of the particles of the lower size range are determined from the dark-field image frame by using the particles of the intermediate size range as internal calibration standards.

Abstract translation: 提供了一种方法和粒子分析器，用于确定包括较小尺寸范围的颗粒，中等尺寸范围的颗粒和大尺寸范围的颗粒的液体样品的粒度分布。 捕获暗场图像帧，其中分辨较小尺寸范围的颗粒和中等尺寸范围的颗粒，并且捕获亮场图像帧，其中中等尺寸范围的颗粒和颗粒的 上限范围解决。 从亮场图像帧确定中等尺寸范围的颗粒的绝对尺寸和较大尺寸范围的颗粒。 通过使用中等尺寸范围的颗粒作为内部校准标准，从暗视野图像帧确定较小尺寸范围的颗粒的校准尺寸。

公开(公告)号：US12023670B2

公开(公告)日：2024-07-02

申请号：US17870563

申请日：2022-07-21

Applicant: ProteinSimple

Inventor： Cheng Zhou ,  Hui Xu ,  Roland Y. Wang ,  David J. Roach ,  Jessica Dermody ,  Tom Weisan Yang ,  Jiaqi Wu

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502753 ,  B01L2200/04 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2400/0406 ,  B01L2400/0421

Abstract: Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.

公开(公告)号：US11953436B2

公开(公告)日：2024-04-09

申请号：US17181610

申请日：2021-02-22

Applicant: ProteinSimple

Inventor： James McElroy ,  Christopher Heger

IPC: G01N21/33 ,  A01N1/02 ,  B01L3/00 ,  C07C309/65 ,  C07C309/73 ,  G01N1/40 ,  G01N21/01 ,  G01N21/31 ,  G01N21/64 ,  G01N27/414 ,  G01N33/52 ,  G01N33/532 ,  G01N33/543 ,  G01N33/569 ,  G01N33/58 ,  G01N33/72 ,  G01N35/04

CPC classification number: G01N21/6428 ,  G01N33/582 ,  G01N2440/20 ,  G01N2500/00

Abstract: Embodiments described herein relate to devices, and methods for quantifying thiol content in a sample containing a mixture of proteins or protein isoforms. The method includes conjugating a portion of the sample with free thiol detection binders, separating the contents in the portion of the sample into separated protein isoforms, detecting fluorescence signals associated with each separated protein isoform, and quantifying, based on the fluorescence signals, a relative amount of free thiol associated with each separated protein isoform. In some instances, the method includes quantifying the amount of each separated protein isoform based on absorbance signals associated with each separated protein isoform. In some instances, the fluorescence and/or absorbance signals associated with protein isoforms conjugated with detection binders can be compared with the corresponding signals associated with unconjugated protein isoforms. In some instances, the method further includes applying a reducing agent and quantifying total-thiol content in the sample.

公开(公告)号：US11933759B2

公开(公告)日：2024-03-19

申请号：US15707600

申请日：2017-09-18

Applicant: ProteinSimple

Inventor： Tom W. Yang ,  David John Roach

IPC: G01N27/447 ,  C07K1/26 ,  C07K1/28

CPC classification number: G01N27/44782 ,  G01N27/44704 ,  C07K1/26 ,  C07K1/285 ,  G01N27/44726 ,  G01N27/44791

Abstract: An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided.

公开(公告)号：US11913904B2

公开(公告)日：2024-02-27

申请号：US17385553

申请日：2021-07-26

Applicant: ProteinSimple

Inventor： Hui Xu ,  Uyen Nguyen

IPC: G01N27/447 ,  G01N27/453

CPC classification number: G01N27/44756 ,  G01N27/44791

Abstract: Embodiments described herein generally relate to cartridges suitable for performing electrophoretic separation of analytes. Cartridges described herein are particularly well suited for reuse. Cartridges described herein can include a reservoir disposed between a capillary and a container containing a run buffer. The reservoir can inhibit run buffer from intruding into the capillary, thereby allowing repeated electrophoretic separations to be more consistent, more accurate, and/or more reliable.

公开(公告)号：US11535900B1

公开(公告)日：2022-12-27

申请号：US17554830

申请日：2021-12-17

Applicant: ProteinSimple

Inventor： Jiaqi Wu ,  Christopher Heger

IPC: A61K38/00 ,  C07K1/00 ,  C07K1/14 ,  C12N9/80 ,  C12N9/82 ,  C12Q1/70 ,  G16B20/00

Abstract: Embodiments disclosed include systems, devices, and methods for analysis of samples containing particles used for gene delivery to determine a quality of the sample and/or an indication that the gene delivery particles are in a full, partial, and/or empty state. The present disclosure also relates to determining a protein and/or NA content in samples with known proportions of gene delivery particles in a full, partial, and/or empty state and based on the determination, establish a relationship between NA content and proportions of gene delivery particles in a full state. The present disclosure also relates to using such an established relationship to predict a proportion of the gene delivery particles in a full, partial, and/or empty state in test samples having the gene delivery particles in an unknown state.