公开(公告)号：US11420202B1

公开(公告)日：2022-08-23

申请号：US17463326

申请日：2021-08-31

Applicant: ProteinSimple

Inventor： Cheng Zhou ,  Hui Xu ,  Roland Y. Wang ,  David J. Roach ,  Jessica Dermody ,  Tom Weisan Yang ,  Jiaqi Wu

IPC: B01L3/00

Abstract: Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.

公开(公告)号：US12023670B2

公开(公告)日：2024-07-02

申请号：US17870563

申请日：2022-07-21

Applicant: ProteinSimple

Inventor： Cheng Zhou ,  Hui Xu ,  Roland Y. Wang ,  David J. Roach ,  Jessica Dermody ,  Tom Weisan Yang ,  Jiaqi Wu

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502753 ,  B01L2200/04 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2400/0406 ,  B01L2400/0421

Abstract: Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.