公开(公告)号：US12023670B2

公开(公告)日：2024-07-02

申请号：US17870563

申请日：2022-07-21

Applicant: ProteinSimple

Inventor： Cheng Zhou ,  Hui Xu ,  Roland Y. Wang ,  David J. Roach ,  Jessica Dermody ,  Tom Weisan Yang ,  Jiaqi Wu

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502753 ,  B01L2200/04 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2400/0406 ,  B01L2400/0421

Abstract: Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.

公开(公告)号：US12216082B2

公开(公告)日：2025-02-04

申请号：US18345798

申请日：2023-06-30

Applicant: ProteinSimple

Inventor： David J. Roach ,  Tom Weisan Yang ,  Hui Xu

IPC: G01N27/447 ,  G01N21/05 ,  G01N30/74

Abstract: A system includes a housing, a cartridge retainer disposed within the housing, a detection assembly disposed within the housing, and a reagent tray holder movably disposed in the housing. The cartridge retainer configured to receive a capillary cartridge having a capillary. The detection assembly includes at least one emitter, a first detector, and a second detector. The detection assembly is configured to transition between a first configuration, in which the first detector detects a first output of the at least one emitter, and a second configuration, in which the second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of the capillary cartridge in fluid communication with a reagent volume.

公开(公告)号：US11726058B2

公开(公告)日：2023-08-15

申请号：US17063123

申请日：2020-10-05

Applicant: ProteinSimple

Inventor： David J. Roach ,  Tom Weisan Yang ,  Hui Xu

IPC: G01N27/453 ,  G01N27/447 ,  G01N21/05 ,  G01N30/74

CPC classification number: G01N27/44791 ,  G01N27/447 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44747 ,  G01N21/05 ,  G01N27/44721 ,  G01N27/44726 ,  G01N27/44795 ,  G01N30/74

Abstract: A system includes a housing, a cartridge retainer disposed within the housing, a detection assembly disposed within the housing, and a reagent tray holder movably disposed in the housing. The cartridge retainer configured to receive a capillary cartridge having a capillary. The detection assembly includes at least one emitter, a first detector, and a second detector. The detection assembly is configured to transition between a first configuration, in which the first detector detects a first output of the at least one emitter, and a second configuration, in which the second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of the capillary cartridge in fluid communication with a reagent volume.

公开(公告)号：US11420202B1

公开(公告)日：2022-08-23

申请号：US17463326

申请日：2021-08-31

Applicant: ProteinSimple

Inventor： Cheng Zhou ,  Hui Xu ,  Roland Y. Wang ,  David J. Roach ,  Jessica Dermody ,  Tom Weisan Yang ,  Jiaqi Wu

IPC: B01L3/00

Abstract: Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.

公开(公告)号：US10794860B2

公开(公告)日：2020-10-06

申请号：US16033808

申请日：2018-07-12

Applicant: ProteinSimple

Inventor： David J. Roach ,  Tom Weisan Yang ,  Hui Xu

IPC: G01N27/453 ,  G01N27/447 ,  G01N21/05 ,  G01N30/74

Abstract: A system includes a housing, a cartridge retainer disposed within the housing, a detection assembly disposed within the housing, and a reagent tray holder movably disposed in the housing. The cartridge retainer configured to receive a capillary cartridge having a capillary. The detection assembly includes at least one emitter, a first detector, and a second detector. The detection assembly is configured to transition between a first configuration, in which the first detector detects a first output of the at least one emitter, and a second configuration, in which the second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of the capillary cartridge in fluid communication with a reagent volume.

公开(公告)号：US08940232B2

公开(公告)日：2015-01-27

申请号：US13778757

申请日：2013-02-27

Applicant: ProteinSimple

Inventor： David J. Roach ,  Tom W. Yang ,  Roger A. O'Neill ,  Robert T. Loder, Jr.

IPC: B01L9/00 ,  B01L3/02 ,  G01N1/14 ,  G01N35/10 ,  F16L41/00 ,  G01N35/04

CPC classification number: F16L41/00 ,  G01N35/04 ,  G01N35/1065 ,  G01N2035/1034 ,  G01N2035/1039 ,  G01N2035/1051 ,  G01N2035/1062 ,  Y10T137/0318 ,  Y10T137/85938 ,  Y10T436/11 ,  Y10T436/119163 ,  Y10T436/2575

Abstract: An automated assay system is described with stations for placement of materials to be used in an assay of materials inside capillaries and an automated gripper for manipulating capillaries. The system includes a separation and immobilization station where reactions inside the capillaries take place and a detector station where photoemissions from the capillary reactions are detected. The photoemissions from the capillaries may be displayed as line graphs or in columns of a pseudo-gel image resembling the familiar Western gel blot. An automated control system has a user interface by which an operator can select a run protocol and define the locations of samples and reagents to be used in the protocol run: Following the setup the control system will cause the automated system to execute the protocol, then display the results in a selected display format.

Abstract translation: 描述了用于放置用于毛细管内部的材料测定的材料的站的自动测定系统和用于操纵毛细管的自动化夹具。 该系统包括分离和固定站，其中毛细管内部发生反应，并且检测到来自毛细血管反应的光摄取的检测器站。 来自毛细血管的光摄取可以显示为线图或类似于熟悉的Western凝胶印迹的假凝胶图像的列。 自动控制系统具有用户界面，操作者可以通过该界面选择运行协议并定义要在协议运行中使用的样本和试剂的位置：设置完成后，控制系统将使自动化系统执行协议，然后 以选定的显示格式显示结果。

公开(公告)号：US20130167937A1

公开(公告)日：2013-07-04

申请号：US13778757

申请日：2013-02-27

Applicant: ProteinSimple

Inventor： David J. Roach ,  Tom W. Yang ,  Roger A. O'Neil ,  Robert T. Loder, JR. ,  Peter B. Vander Horn

IPC: F16L41/00

CPC classification number: F16L41/00 ,  G01N35/04 ,  G01N35/1065 ,  G01N2035/1034 ,  G01N2035/1039 ,  G01N2035/1051 ,  G01N2035/1062 ,  Y10T137/0318 ,  Y10T137/85938 ,  Y10T436/11 ,  Y10T436/119163 ,  Y10T436/2575

Abstract: An automated assay system is described with stations for placement of materials to be used in an assay of materials inside capillaries and an automated gripper for manipulating capillaries. The system includes a separation and immobilization station where reactions inside the capillaries take place and a detector station where photoemissions from the capillary reactions are detected. The photoemissions from the capillaries may be displayed as line graphs or in columns of a pseudo-gel image resembling the familiar Western gel blot. An automated control system has a user interface by which an operator can select a run protocol and define the locations of samples and reagents to be used in the protocol run: Following the setup the control system will cause the automated system to execute the protocol, then display the results in a selected display format.

Abstract translation: 描述了用于放置用于毛细管内部的材料测定的材料的站的自动测定系统和用于操纵毛细管的自动化夹具。 该系统包括分离和固定站，其中毛细管内部发生反应，并且检测到来自毛细血管反应的光摄取的检测器站。 来自毛细血管的光摄取可以显示为线图或类似于熟悉的Western凝胶印迹的假凝胶图像的列。 自动控制系统具有用户界面，操作者可以通过该界面选择运行协议并定义要在协议运行中使用的样本和试剂的位置：设置完成后，控制系统将使自动化系统执行协议，然后 以选定的显示格式显示结果。