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公开(公告)号：US20220356206A1
公开(公告)日：2022-11-10
申请号：US17872326
申请日：2022-07-25
IPC分类号： C07K1/36 , C07K1/34 , C07K1/22 , C07K1/26 , B01D69/10 , B01D15/38 , B01D57/02 , B01D15/20 , B01D15/18 , B01D71/68 , B01D63/10 , B01D69/02 , B01D69/14 , C07K16/00 , B01D59/10
摘要： Provided herein are, inter alia, biological manufacturing and downstream purification processes.
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公开(公告)号：US10962537B2
公开(公告)日：2021-03-30
申请号：US16095653
申请日：2017-04-21
申请人： Memphasys Limited
IPC分类号： B01D57/02 , B01D69/02 , B01D71/44 , B01D67/00 , B01D71/38 , C07K1/26 , G01N33/561 , B01D69/10 , B01D71/52
摘要： The present invention relates to polymeric membranes. In particular, the present invention relates to the use of membranes comprising polyvinyl alcohol in electrophoresis.
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3.发明申请公开(公告)号：US20190389902A1
公开(公告)日：2019-12-26
申请号：US16281649
申请日：2019-02-21
发明人： Paul T. Nix , Marcus J. Horn
IPC分类号： C07K1/26 , C08F220/56 , G01N27/447
摘要： A method of collecting one or more target macromolecules in a capture membrane by gel electrophoresis is disclosed, as well as a kit for macromolecule isolation and recovery including: a preformed gel; a capture device; an insertion guide; and optionally, a migration gauge.
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公开(公告)号：US10352897B2
公开(公告)日：2019-07-16
申请号：US15607403
申请日：2017-05-26
发明人： Thomas Jackson
摘要： An expanding cam lock for use with an electrophoresis system is disclosed herein. The cam lock allows the simultaneous use of multiple slab gel cassettes in first and second buffer core assemblies in an electrophoresis system while maintaining the necessary compressive force to create a liquid-tight seal between the anode and cathode buffer solutions. In one example embodiment, the expanding cam lock includes a base plate with a first surface adapted to engage the first buffer core assembly and a follower plate having second surface adapted to engage the second buffer core assembly, buffer dam or buffer displacement dam. The base plate and the follower plate are slidably coupled together and are designed for insertion between the first buffer core assembly and the second buffer core assembly, buffer dam or buffer displacement dam in the electrophoresis container. A cam is positioned between and moveably coupled with the base plate and the follower plate. The cam is movable from a first position to a second position to urge the first and second surfaces to secure the gel cassette to the first and second buffer core assemblies. Also provided herein is a buffer displacement dam. Also provided herein are kits and assemblies which incorporate the expanding cam and buffer displacement dam described herein.
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公开(公告)号：US10156546B2
公开(公告)日：2018-12-18
申请号：US14768986
申请日：2014-02-19
IPC分类号： G01N27/447 , G01N27/327 , B01L3/00 , F04B19/00 , G01N33/483 , C07K1/26
摘要： A device for the detection of target entities, the device including: a fluidic channel to conduct a liquid sample containing at least one type of target entity to be detected; and mutually spaced electrodes disposed along the fluidic channel; wherein the mutually spaced electrodes are functionalized to selectively bond to the at least one type of target entity and are configured to electro-hydrodynamically pump the liquid sample along the fluidic channel on application of a signal to the electrodes, such that selectivity of attachment of the at least one type of target entity to the electrodes is determined by at least one parameter of the signal.
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公开(公告)号：US10072041B2
公开(公告)日：2018-09-11
申请号：US13985369
申请日：2012-02-15
申请人： Oleg Melnyk , Nathalie Ollivier , Reda Mhidia , Julien Dheur
发明人： Oleg Melnyk , Nathalie Ollivier , Reda Mhidia , Julien Dheur
摘要： Method for preparing a peptide assembly of n fragments and n−1 amino acids bearing a thiol function, represented by the formula: A1-C1-A2-C2-A3- . . . -Ci−1-Ai- . . . -Cn−1-An (I) in which A1, A2, A3, . . . Ai . . . , An are peptide fragments, C1, C2, C3 . . . Ci−1 . . . Cn−1 are amino acid residues bearing a thiol function, n is comprised between 3 and 50, and i is 2 to n, in which a peptide-thioester is prepared of formula: A1-SR (II) in which A1 is a peptide fragment and SR is an alkyl thioester residue, R being alkyl optionally substituted, starting from a bis(2-sulphanylethyl)amino peptide.
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7.发明申请公开(公告)号：US20180088080A1
公开(公告)日：2018-03-29
申请号：US15716149
申请日：2017-09-26
IPC分类号： G01N27/447 , B01L9/06 , B01D59/42 , C07K1/26
CPC分类号： G01N27/44743 , B01D59/42 , B01L9/065 , B82B1/00 , C07K1/26 , C25B7/00 , G01N27/4473 , G01N27/44782 , G01N30/7266 , G01N2035/00237
摘要： The combined capillary electrophoresis electrospray mass spectrometry apparatus has a circuit to handle excess current allows separations under a wide range of electrophoretic conditions. The apparatus includes an electrospray with an emitter and an electrospray interface connected with a separation capillary configured to transport a sample with an injection end and a distal end. The injection end of the separation capillary is inserted into a reservoir containing a background electrolyte and the distal end is threaded within the electrospray interface and sized and shaped to mate with the electrospray interface. A power supply is electrically connected to the injection end and an amplifier at least one first diode positioned between the amplifier and the distal end allows current to flow to the distal end only. A second diode positioned between the distal end and a ground configured to allow current flow to the ground.
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公开(公告)号：US09733212B2
公开(公告)日：2017-08-15
申请号：US13715812
申请日：2012-12-14
发明人： Roumen Bogoev , Douglas Kang
IPC分类号： G01N27/416 , C07K1/13 , C07K1/26
CPC分类号： G01N27/4163 , C07K1/13 , C07K1/26 , Y10T436/105831
摘要： Pre-labeled protein standards useful in electrophoresis that have sharp, consistent separation characteristics that are substantially the same as those of their unlabeled counterparts are provided. The invention provides pre-labeled protein standard sets that include a plurality of labeled proteins that are labeled on a first amino acid, in which side reactions of the label with amino acids not targeted for labeling are reduced.
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公开(公告)号：US09709526B2
公开(公告)日：2017-07-18
申请号：US14258160
申请日：2014-04-22
IPC分类号： G01N27/447 , C07K1/26
CPC分类号： G01N27/447 , C07K1/26 , G01N27/44747
摘要： A method of characterizing proteins has been developed that includes providing a sample that contains a plurality of proteins to be characterized, wherein at least a first protein of the plurality of proteins is in its native form. Additionally, the method includes contacting the sample containing the plurality of proteins with a solution to form a sample solution, and then contacting the sample solution with a gel. The plurality of proteins is subsequently separated via electrophoresis within the gel, which includes an electrophoresis solution.
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公开(公告)号：US20150226703A1
公开(公告)日：2015-08-13
申请号：US14577824
申请日：2014-12-19
发明人： Shane Petersen , Cory Panattoni , Craig Rowell
IPC分类号： G01N27/447 , C07K1/26
CPC分类号： G01N27/44747 , C07K1/26 , Y10S436/826
摘要： Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components.
摘要翻译： 提供高分辨率的聚丙烯酰胺凝胶作为蛋白质分离的电泳介质，并且通过包含牛磺酸，天冬酰胺或两者作为两性电解质与三（羟甲基） - 氨基甲烷或双（2- 羟乙基）氨基 - 三（羟甲基）甲烷作为缓冲液，加上其他常规组分。
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