公开(公告)号：US08945360B2

公开(公告)日：2015-02-03

申请号：US12691440

申请日：2010-01-21

申请人： Shane Petersen ,  Cory Panattoni ,  Craig Rowell

发明人： Shane Petersen ,  Cory Panattoni ,  Craig Rowell

IPC分类号： C07K1/26 ,  G01N27/447

CPC分类号： G01N27/44747 ,  C07K1/26 ,  Y10S436/826

摘要： Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components.

摘要翻译： 提供高分辨率的聚丙烯酰胺凝胶作为蛋白质分离的电泳介质，并且通过包含牛磺酸，天冬酰胺或两者作为两性电解质与三（羟甲基） - 氨基甲烷或双（2- 羟乙基）氨基 - 三（羟甲基）甲烷作为缓冲液，加上其他常规组分。

公开(公告)号：US06777246B2

公开(公告)日：2004-08-17

申请号：US10025378

申请日：2001-12-18

申请人： Christopher C. Lawrence ,  Armen B. Shanafelt

发明人： Christopher C. Lawrence ,  Armen B. Shanafelt

IPC分类号： G01N33546

CPC分类号： G01N33/54346 ,  G01N33/5306 ,  G01N33/54393 ,  Y10S436/825 ,  Y10S436/826

摘要： A reagent for use in immunoassays reduces interference in particle agglutination assays. The reagent contains particles having covalently bound antibodies and a tertiary amine compound of formula (I): N(R1—X)(R2—Y)(R3—Z)  (I). The moieties R1, R2, and R3 are independently alkyl or alkyl ether. The moieties X, Y, and Z are independently —OH, —O—R4, —S—R4, —C(═O)—OH, —C(═O)—OR4, or —C(═O)—NHR4 (R4 is alkyl).

摘要翻译： 用于免疫测定的试剂降低了颗粒凝集测定中的干扰。 试剂含有具有共价结合抗体的颗粒和式（I）的叔胺化合物：部分R 1，R 2和R 3独立地为烷基或烷基醚。 部分X，Y和Z独立地为-OH，-OR 4，-SR 4，-C（= O）-OH，-C（= O）-OR 4或-C（ = O）-NHR 4（R 4是烷基）。

公开(公告)号：US06740527B1

公开(公告)日：2004-05-25

申请号：US08863121

申请日：1997-05-27

申请人： Show-Chu Wong ,  Sylvia Khoo

发明人： Show-Chu Wong ,  Sylvia Khoo

IPC分类号： G01N3348

CPC分类号： G01N33/5094 ,  G01N33/721 ,  Y10S435/962 ,  Y10S435/967 ,  Y10S436/826 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/115831 ,  Y10T436/2525

摘要： Cyanide-free reagents for the determination of hemoglobin and leukocytes present in a blood sample comprise an aqueous solutions of at least one quaternary ammonium salt, preferably selected from the group of alkyltrimethylammonium salts, alkyldimethylbenzylammonium salts or alkylpyridium salts consisting of tetradecyltrimethyl ammonium bromide (TTAB), dodecyltrimethyl ammonium chloride, cetyltrimethyl ammonium bromide, hexadecyltrimethyl ammonium bromide and benzalkonium chlorides, and hydroxylamine salts, especially hydrochloride, sulfate and phosphates and other acid salts. The method involves mixing the reagent with a diluent-diluted blood sample, presenting it to an absorbance spectrophotometer and measuring the resulting optical density as an indicator of hemoglobin concentration. This cyanide-free reagent could be used solely for hemoglobin determinations, or, it can also be used in leukocyte counting and sizing using hematology instrumentation.

摘要翻译： 用于测定存在于血液样品中的血红蛋白和白细胞的无氰试剂包含至少一种季铵盐的水溶液，优选选自烷基三甲基铵盐，烷基二甲基苄基铵盐或由十四烷基三甲基溴化铵（TTAB）组成的烷基吡啶盐， 十六烷基三甲基氯化铵，十六烷基三甲基溴化铵，十六烷基三甲基溴化铵和苯扎氯铵，以及羟胺盐，特别是盐酸盐，硫酸盐和磷酸盐等酸盐。 该方法包括将试剂与稀释剂稀释的血液样品混合，将其呈现给吸光度分光光度计，并测量所得光密度作为血红蛋白浓度的指标。 该无氰试剂可用于血红蛋白测定，也可用于使用血液学仪器进行白细胞计数和上浆。

公开(公告)号：US20040096900A1

公开(公告)日：2004-05-20

申请号：US10433923

申请日：2003-12-17

发明人： David Laurie ,  Alexander Kirkley Barnes ,  Michael James Gardner

IPC分类号： G01N033/53

CPC分类号： G01N33/82 ,  Y10S436/817 ,  Y10S436/825 ,  Y10S436/826 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255

摘要： The present invention relates to a method for measuring the amount of vitamin D metabolite in a sample of plasma or serum, comprising the step of (a) adding to a serum or plasma sample a non-competitive displacement agent to effect separation of any vitamin D metabolite in the sample form protein to which it is bound, such that any vitamin D metabolite may be detected and/or measured; and (b) detecting or measuring the amount of vitamin D metabolite in the sample of (a). Such methods are useful in determining the vitamin D status of the subject, for example in the diagnosis of disease. Also provide are agents and kits for use in performing the methods of the invention.

摘要翻译： 本发明涉及一种用于测量血浆或血清样品中维生素D代谢物的量的方法，其包括以下步骤：（a）向血清或血浆样品中加入非竞争性置换剂以分解任何维生素D 样品中的代谢物形成与其结合的蛋白质，使得可以检测和/或测量任何维生素D代谢物; 和（b）检测或测量（a）样品中维生素D代谢物的量。 这样的方法可用于确定受试者的维生素D状态，例如在疾病的诊断中。 还提供用于实施本发明方法的试剂和试剂盒。

公开(公告)号：US20020160353A1

公开(公告)日：2002-10-31

申请号：US10099498

申请日：2002-03-13

申请人： Quest Diagnostics Investments Incorporated

发明人： Lata Sundaram ,  Ann Hoang ,  Stan Shimizu ,  Elizabeth R. Umelo-Njaka ,  Darcy A. Thomas

IPC分类号： A01N001/02 ,  C12N009/00

CPC分类号： G01N33/78 ,  Y10S436/804 ,  Y10S436/817 ,  Y10S436/826 ,  Y10T436/105831 ,  Y10T436/108331

摘要： This invention relates to preservative solutions for peptides, for example, parathyroid hormone, insulin-like growth factor binding protein 3 (IGFBP3), adrenocorticotropic hormone (ACTH) and mixtures thereof. Preferably, the peptides preserved by the solutions are non-reconstituted. In one embodiment, the preservative solutions comprise a polyvinyl alcohol, EDTA component and molybdic component.

摘要翻译： 本发明涉及肽的防腐溶液，例如甲状旁腺激素，胰岛素样生长因子结合蛋白3（IGFBP3），促肾上腺皮质激素（ACTH）及其混合物。 优选地，由溶液保存的肽不被重构。 在一个实施方案中，防腐剂溶液包含聚乙烯醇，EDTA组分和钼组分。

公开(公告)号：US06423505B1

公开(公告)日：2002-07-23

申请号：US09406013

申请日：1999-09-24

申请人： Kenneth A. Davis

发明人： Kenneth A. Davis

IPC分类号： G01N3353

CPC分类号： G01N33/56977 ,  C07K16/2896 ,  G01N33/56972 ,  G01N2333/70539 ,  G01N2333/70553 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/80 ,  Y10S436/808 ,  Y10S436/826

摘要： Improved methods, reagents, and kits for quantitation of HLA-DR and/or CD11b expression on peripheral blood cells are presented. Inclusion of a lysosomotropic amine, such as chloroquine, during staining stabilizes HLA-DR and CD11b expression. Use of a novel anti-CD14 conjugate, anti-CD14-PerCP/CY5.5, permits the ready discrimination of monocytes. The improved methods, reagents, and kits may be used to assess immune competence, and to direct and monitor immunostimulatory therapies in septic patients exhibiting monocyte deactivation.

摘要翻译： 提出了用于定量外周血细胞上HLA-DR和/或CD11b表达的改进的方法，试剂和试剂盒。 在染色期间包含溶酶体胺，如氯喹，稳定了HLA-DR和CD11b的表达。 使用抗CD14结合物，抗CD14-PerCP / CY5.5，可以准确鉴别单核细胞。 可以使用改进的方法，试剂和试剂盒来评估免疫能力，并且指导和监测显示单核细胞失活的脓毒症患者的免疫刺激疗法。

公开(公告)号：US06337178B2

公开(公告)日：2002-01-08

申请号：US09843866

申请日：2001-04-30

申请人： Tomoko Takeshita ,  Jun Otomo

发明人： Tomoko Takeshita ,  Jun Otomo

IPC分类号： C12M300

CPC分类号： G01F1/00 ,  G01N2333/726 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/826 ,  Y10T436/108331

摘要： Histamine may be quantitatively measured by performing the following steps. First, an oocyte that expresses histamine receptors is held in a recess formed at the bottom of a vessel. Then, first and second electrodes are inserted into the oocyte. Subsequently, the membrane potential of the oocyte is measured by using the first electrode to stabilize this membrane potential at a predetermined level by driving a current through the second electrode using circuitry for clamping the membrane potential of the oocyte. A sample is then infused into a fine reacting tube having an antigen immobilized on its inner surface together with some buffer solution to promote a histamine releasing reaction. The solution containing histamines that is released in the fine reacting tube is transferred to the vessel to make contact with the oocyte in the vessel. Finally, an electric response of the oocyte caused by the contact with the solution is detected and the concentration of the histamine released in the histamine releasing reaction in the fine reacting tube is determined. The entire blood or mast cell suspension may be used as a sample without pretreatment.

摘要翻译： 组胺可以通过以下步骤进行定量测定。 首先，表达组胺受体的卵母细胞被保持在形成于血管底部的凹陷中。 然后，将第一和第二电极插入卵母细胞。 随后，通过使用第一电极通过使用用于钳位卵母细胞的膜电位的电路驱动通过第二电极的电流来将该膜电位稳定在预定水平来测量卵母细胞的膜电位。 然后将样品输入到具有固定在其内表面上的抗原与一些缓冲溶液的细小反应管中，以促进组胺释放反应。 将包含在细反应管中释放的组胺的溶液转移到容器中以与容器中的卵母细胞接触。 最后，检测由与溶液接触引起的卵母细胞的电响应，并确定微反应管中组胺释放反应中释放的组胺浓度。 整个血液或肥大细胞悬浮液可以用作样品而不进行预处理。

公开(公告)号：US6071522A

公开(公告)日：2000-06-06

申请号：US460040

申请日：1995-06-02

申请人： Wayne Robert Thomas ,  Geoffrey Alexander Stewart ,  Keven J. Turner ,  Richard John Simpson

发明人： Wayne Robert Thomas ,  Geoffrey Alexander Stewart ,  Keven J. Turner ,  Richard John Simpson

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K39/00 ,  A61K39/35 ,  A61P37/00 ,  A61P37/08 ,  C07G99/00 ,  C07H21/04 ,  C07K14/00 ,  C07K14/435 ,  C07K19/00 ,  C12N1/20 ,  C12N1/21 ,  C12N15/00 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12R1/19 ,  G01N33/564

CPC分类号： C07K14/43531 ,  A61K38/00 ,  A61K39/00 ,  Y10S436/826

摘要： Recombinant DNA expression vectors or cloning vehicles capable of expressing a polypeptide displaying the antigenicity of an allergen of house dust mites of the genus Dermatophagoides. A synthetic protein or polypeptide displaying antigenicity of all or a portion of an allergen of house dust mites of the genus Dermatophagoides. Methods of diagnosing and treating such allergies.

摘要翻译： 重组DNA表达载体或能够表达显示Dermatophagoides属的房尘螨变应原的抗原性的多肽的克隆载体。 显示Dermatophagoides属的尘螨全部或部分变应原的抗原性的合成蛋白质或多肽。 诊断和治疗这种过敏症的方法。

公开(公告)号：US6004762A

公开(公告)日：1999-12-21

申请号：US892488

申请日：1997-07-14

申请人： Doris B. Tse ,  Hui-Min Chung ,  Leonardus H. T. Van der Ploeg

发明人： Doris B. Tse ,  Hui-Min Chung ,  Leonardus H. T. Van der Ploeg

IPC分类号： G01N1/30 ,  G01N33/50 ,  G01N33/569 ,  G01N33/574

CPC分类号： G01N33/56966 ,  G01N1/30 ,  G01N33/5005 ,  G01N33/57492 ,  Y10S435/96 ,  Y10S435/962 ,  Y10S436/813 ,  Y10S436/826 ,  Y10T436/25 ,  Y10T436/2525

摘要： This invention provides a method for preserving cells which comprises the steps of (a) suspending cells in a physiologically-acceptable, isotonic medium; and (b) fixing the cells so suspended at a temperature of less than about 10.degree. C. under sufficiently hypertonic conditions so as to disperse the cells in a single, unagglutinated state, thereby preserving cells. This invention also provides a method for detecting cells separated from a sample which have been preserved according to the aforementioned method. This invention also provides a method for visualizing cells. Also provided is a method for detecting a metabolic process in cells present in a sample. This invention also provides a method for detecting the presence of rare cells in a sample which specifically possess on their surfaces a moiety recognized by a known ligand comprising preserving cells separated from the sample according to the aforementioned method for preserving cells. The subject invention also provides for quantitatively determining and isolating rare cells detected according to the aformentioned method. Further provided are methods for determining whether a fetus is afflicted with a genetic abnormality, determining the gender of a fetus, determining whether a subject is afflicted with a tumor, and detecting in a subject the presence of cells expressing a malignant phenotype, each method comprising preserving cells according to the aforementioned method for preserving cells.

摘要翻译： 本发明提供了一种保存细胞的方法，其包括以下步骤：（a）将细胞悬浮在生理上可接受的等渗介质中; 和（b）在足够高渗条件下将细胞悬浮在小于约10℃的温度下，以将细胞分散在单一未凝集状态，从而保留细胞。 本发明还提供一种根据上述方法检测从样品分离的细胞的方法。 本发明还提供了一种可视化细胞的方法。 还提供了用于检测样品中存在的细胞中的代谢过程的方法。 本发明还提供了一种用于检测样品中稀有细胞的存在的方法，该样品在其表面上具有由已知配体识别的部分，其包含根据上述保存细胞的方法从样品分离的保存细胞。 本发明还提供了定量测定和分离根据上述方法检测的稀有细胞。 进一步提供的方法是确定胎儿是否患有遗传异常，确定胎儿的性别，确定受试者是否患有肿瘤，以及在受试者中检测表达恶性表型的细胞的存在，每种方法包括 根据上述保存细胞的方法保存细胞。

公开(公告)号：US5773212A

公开(公告)日：1998-06-30

申请号：US734435

申请日：1996-10-16

申请人： Steve David Figard

发明人： Steve David Figard

IPC分类号： G01N33/53 ,  G01N33/531 ,  G01N33/543 ,  G01N33/576

CPC分类号： G01N33/531 ,  G01N33/5306 ,  G01N33/54393 ,  G01N33/5767 ,  Y10S436/82 ,  Y10S436/826

摘要： An aqueous composition suitable for use when performing immunological procedures. The composition includes at least one biological buffer, dithiothreitol (alternatively referred to herein as "DTT"), and ethylene glycol. The composition can also include at least one biological detergent, at least one source of positive and negative counterions, e.g., salt, and at least one viscosity modifier, e.g., sugar. The buffer also can include at least one preservative, such as sodium azide. The pH of the composition preferably ranges from about 6.4 to 7.2. A kit containing the composition is also disclosed.

摘要翻译： 适合在进行免疫程序时使用的水性组合物。 组合物包括至少一种生物缓冲液，二硫苏糖醇（本文中称为“DTT”）和乙二醇。 组合物还可以包括至少一种生物洗涤剂，至少一种正和负抗衡离子源，例如盐，和至少一种粘度调节剂，例如糖。 缓冲液还可以包括至少一种防腐剂，例如叠氮化钠。 组合物的pH优选为约6.4至7.2。 还公开了含有该组合物的试剂盒。