公开(公告)号：US12018240B2

公开(公告)日：2024-06-25

申请号：US17562815

申请日：2021-12-27

申请人： STEMCELL TECHNOLOGIES CANADA INC.

发明人： Maisam Dadgar ,  Jacquelyn L. Sikora Hanson ,  Armon R. Sharei

IPC分类号： G01N33/68 ,  B01L3/00 ,  C07C309/65 ,  C07C309/73 ,  C12M1/26 ,  C12M3/06 ,  G01N21/33 ,  G01N21/64 ,  G01N33/52

CPC分类号： C12M23/16 ,  B01L3/502707 ,  C12M33/12 ,  B01L2200/12 ,  B01L2300/0819

摘要： A microfluidic chip for causing the delivery of a payload to a cell comprises a plurality of constrictions configured to allow a cell suspension to flow through one or more of the plurality of constrictions from a first fluid flow region to a second fluid flow region within the microfluidic chip, wherein a cross-sectional width of each of the plurality of constrictions is less than a diameter of cells in the cell suspension, such that membranes of the cells are perturbed when passing through the constrictions such that a payload is able to pass through the perturbed cell membranes, and wherein a quotient of a cross-sectional area over a cross-sectional perimeter of each of the plurality of constrictions is greater than greater than or equal to 0.5 μm.

公开(公告)号：US12017226B2

公开(公告)日：2024-06-25

申请号：US18298971

申请日：2023-04-11

申请人： Ancestry.com DNA, LLC

发明人： Thomas E. Blair ,  Tyler Fredric Allan ,  Bryce Twede ,  Jeremy Johnson

IPC分类号： G01N33/68 ,  A01N1/02 ,  B01L3/00 ,  B81B1/00 ,  B81C1/00 ,  C07C309/65 ,  C07C309/73 ,  F04B43/12 ,  G01N1/40 ,  G01N21/33 ,  G01N21/64 ,  G01N33/52 ,  G01N35/10

CPC分类号： B01L3/523 ,  B01L3/50825 ,  B01L2200/0689 ,  B01L2200/087 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/049 ,  B01L2300/0832

摘要： A sample collection system can include a sample collection vessel having a sample collection chamber with an opening configured to receive a sample into the sample collection chamber. The sample collection system can additionally include a selectively movable sleeve valve configured to associate with the opening of the sample collection chamber. The sample collection system can include a sealing cap that is configured to associate with the selectively movable sleeve valve and with the sample collection vessel. The sealing cap can include a reagent chamber having reagent(s) stored therein, and when the sealing cap is associated with the sample collection vessel, the selectively movable sleeve valve opens, dispensing the reagent(s) into the sample collection chamber. When the selectively moveable sleeve associates with the sample collection chamber, an outer sleeve slides relative to an inner vessel, opening the sleeve and dispensing reagent into the sample collection chamber.

公开(公告)号：US12005445B2

公开(公告)日：2024-06-11

申请号：US17323228

申请日：2021-05-18

申请人： KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY

发明人： Bin Xin ,  Iman S. Roqan

IPC分类号： G01N33/68 ,  A01N1/02 ,  B01L3/00 ,  B81B1/00 ,  B81C1/00 ,  C07C309/65 ,  C07C309/73 ,  F04B43/12 ,  G01N1/40 ,  G01N21/33 ,  G01N21/64 ,  G01N33/52 ,  G01N35/10

CPC分类号： B01L3/502715 ,  B81B1/006 ,  B81C1/00119 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0819 ,  B81B2201/05 ,  B81B2203/033 ,  B81B2203/0338 ,  B81B2203/04 ,  B81C2201/0197

摘要： A method for making ion-crystal semiconductor material based micro- and/or nanowires, MNWs, embedded into a semiconductor substrate, includes forming a structure into the semiconductor substrate, wherein the structure has each of a width and a depth less than 10 μm; pumping an ion-crystal semiconductor material as an ion solution into the structure, wherein the pumping is achieved exclusively due to capillary forces; flowing the ion solution through the structure to fill the structure; crystallizing the ion-crystal semiconductor material inside the structure to form the MNWs; and adding electrodes to ends of the MNWs.

公开(公告)号：US11977024B2

公开(公告)日：2024-05-07

申请号：US17644504

申请日：2021-12-15

申请人： MLS ACQ, Inc.

发明人： Martin L. Spartz

IPC分类号： G01N33/68 ,  A01N1/02 ,  B01J19/00 ,  B01J23/42 ,  B01L3/00 ,  C07C309/65 ,  C07C309/73 ,  G01N1/40 ,  G01N21/01 ,  G01N21/31 ,  G01N21/33 ,  G01N21/35 ,  G01N21/3504 ,  G01N21/64 ,  G01N27/414 ,  G01N33/52 ,  G01N33/532 ,  G01N33/543 ,  G01N33/569 ,  G01N33/72 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10

CPC分类号： G01N21/3504 ,  B01J19/0013 ,  B01J23/42 ,  G01N2021/3595

摘要： A gas analysis system and method using a spectrometer, such as a Fourier transform infrared spectrometer, utilizes a reactor, such as a catalytic reactor, for providing interference spectra. The gas is pressurized and chilled to remove water prior to the spectrometer.

公开(公告)号：US11965878B2

公开(公告)日：2024-04-23

申请号：US17171829

申请日：2021-02-09

申请人： Southern Methodist University

发明人： Ali Beskok ,  Anil Koklu ,  Jungchih Chiao

IPC分类号： G01N33/68 ,  B01L3/00 ,  B61L3/24 ,  C07C309/65 ,  C07C309/73 ,  C08J9/236 ,  C11D3/384 ,  G01N21/33 ,  G01N21/64 ,  G01N27/02 ,  G01N27/447 ,  G01N33/487 ,  G01N33/52 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/5302 ,  B01L3/502 ,  C11D3/3845 ,  G01N27/028 ,  G01N27/44726 ,  G01N33/48721 ,  G01N33/5438 ,  B01J2219/00813 ,  B01L2300/0645 ,  B01L2300/088 ,  B01L2300/161 ,  B61L3/243 ,  C08J9/236

摘要： An apparatus and method for detecting one or more target molecules includes a hydrophobic substrate, and a sensor. The sensor includes two or more electrodes disposed on the hydrophobic substrate and separated from one another by a gap, a plurality of nanostructures formed on or within an upper surface of each electrode, a plurality of binding molecules attached to the plurality of nanostructures, wherein the plurality of binding molecules are configured to bind with the one or more target molecules, and wherein the upper surface of each electrode and the plurality of nanostructures are hydrophilic, and may further detect two or more analytes with two or more sensors that detect two or more different modalities, such as, electrical, optical fluorescence, optical resonance, magnetic detection, or acoustic waves.

公开(公告)号：US11953436B2

公开(公告)日：2024-04-09

申请号：US17181610

申请日：2021-02-22

申请人： ProteinSimple

发明人： James McElroy ,  Christopher Heger

IPC分类号： G01N21/33 ,  A01N1/02 ,  B01L3/00 ,  C07C309/65 ,  C07C309/73 ,  G01N1/40 ,  G01N21/01 ,  G01N21/31 ,  G01N21/64 ,  G01N27/414 ,  G01N33/52 ,  G01N33/532 ,  G01N33/543 ,  G01N33/569 ,  G01N33/58 ,  G01N33/72 ,  G01N35/04

CPC分类号： G01N21/6428 ,  G01N33/582 ,  G01N2440/20 ,  G01N2500/00

摘要： Embodiments described herein relate to devices, and methods for quantifying thiol content in a sample containing a mixture of proteins or protein isoforms. The method includes conjugating a portion of the sample with free thiol detection binders, separating the contents in the portion of the sample into separated protein isoforms, detecting fluorescence signals associated with each separated protein isoform, and quantifying, based on the fluorescence signals, a relative amount of free thiol associated with each separated protein isoform. In some instances, the method includes quantifying the amount of each separated protein isoform based on absorbance signals associated with each separated protein isoform. In some instances, the fluorescence and/or absorbance signals associated with protein isoforms conjugated with detection binders can be compared with the corresponding signals associated with unconjugated protein isoforms. In some instances, the method further includes applying a reducing agent and quantifying total-thiol content in the sample.

公开(公告)号：US11859027B2

公开(公告)日：2024-01-02

申请号：US16741821

申请日：2020-01-14

申请人： CARBON, INC.

发明人： John R. Tumbleston ,  Ariel M. Herrmann ,  Matthew Panzer ,  Tabor Baranti

IPC分类号： G01N33/68 ,  G01N21/33 ,  G01N21/64 ,  G01N33/52 ,  C07C309/65 ,  C07C309/73 ,  A01N1/02 ,  G01N1/40 ,  C08F2/01 ,  C09D11/101 ,  H05B6/06 ,  G03F7/09 ,  C08F2/48 ,  B29C64/295 ,  B29C64/393 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y40/00 ,  B33Y50/02 ,  B33Y70/00

CPC分类号： C08F2/01 ,  B29C64/295 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y40/00 ,  B33Y50/02 ,  B33Y70/00 ,  C08F2/48 ,  C09D11/101 ,  G03F7/09 ,  H05B6/06

摘要： A test cell useful for determining the photosensitivity of a photopolymerizable material, includes a support plate having a top surface portion, a bottom surface portion, and an opening extending therebetween, a light-transmissive base member removably or permanently connected to said bottom surface portion to form with said opening a well, into which well photopolymerizable material can be deposited, and a sensor comprising a thermal sensor, strain sensor, or combination thereof operatively associated with said light-transmissive base member.

公开(公告)号：US11851410B2

公开(公告)日：2023-12-26

申请号：US16652733

申请日：2018-10-01

申请人： THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO

发明人： Patrick Thomas Gunning ,  Dziyana Kraskouskaya ,  Aaron Cabral ,  Bronte Murcar-Evans ,  Krimo Toutah ,  Elvin De Araujo

IPC分类号： C07D417/00 ,  C07D257/02 ,  C07C233/44 ,  C07C309/65 ,  C07D213/38 ,  G01N21/64

CPC分类号： C07D257/02 ,  C07C233/44 ,  C07C309/65 ,  C07D213/38 ,  G01N21/64

摘要： This application relates to a method of detecting negatively charged phosphate-containing membranes and membrane components, such as in cell membranes or artificial lipid vesicles, and its use, for example, in detecting apoptosis and bacterial infection.

公开(公告)号：US20230411688A1

公开(公告)日：2023-12-21

申请号：US18030975

申请日：2021-11-30

申请人： LG ENERGY SOLUTION, LTD.

发明人： Jeong-Beom LEE ,  Ye-Eun KIM ,  Seok-Koo KIM ,  Je-Young KIM

IPC分类号： H01M10/0567 ,  H01M10/0568 ,  H01M10/0569 ,  H01M10/0525 ,  C07C309/65 ,  C07C309/06

CPC分类号： H01M10/0567 ,  H01M10/0568 ,  H01M10/0569 ,  H01M2300/0037 ,  C07C309/65 ,  C07C309/06 ,  H01M2300/0028 ,  H01M10/0525

摘要： An electrolyte for a lithium secondary battery including a wettability-improving additive having a trifluoromethylsulfonyl group. The electrolyte has reduced surface tension to improve the wettability of an electrode assembly with the electrolyte. Therefore, it is possible to ensure high electrolyte wettability even when the electrolyte includes a high-boiling point solvent or a high concentration of lithium salt.

公开(公告)号：US11845079B2

公开(公告)日：2023-12-19

申请号：US17643324

申请日：2021-12-08

申请人： International Business Machines Corporation

发明人： Vince Siu ,  Sufi Zafar ,  Benjamin Hardy Wunsch

IPC分类号： G01N33/68 ,  G01N21/33 ,  G01N21/64 ,  G01N33/52 ,  C07C309/65 ,  C07C309/73 ,  A01N1/02 ,  G01N1/40 ,  B01L3/00 ,  C12N15/11 ,  B82Y5/00

CPC分类号： B01L3/502715 ,  B01L3/50273 ,  B01L3/502753 ,  C12N15/111 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/0663 ,  B01L2300/087 ,  B01L2300/0838 ,  B01L2300/0864 ,  B01L2400/043 ,  B01L2400/06 ,  B82Y5/00 ,  C12N2310/20

摘要： A structure, apparatus, and method are disclosed. A silicon substrate with a microfluidic system that receives a sample fluid and prepares an analyte solution received by a reservoir containing a sensing surface is electrically connected to a semiconductor device. The structure includes a component for receiving a sample fluid, a component for preparing the sample fluid, a CRISPR system for cleaving a reporter species when the sample fluid contains a target sequence, a sorting component for separating the cleaved reporter species from the sample fluid and the CRISPR products, a cavity for receiving an analyte solution containing the cleaved reporter species, and a sensing surface in the cavity. The sensing surface detects electrical signals induced by reaction events in the analyte solution.