公开(公告)号：US20240192164A1

公开(公告)日：2024-06-13

申请号：US18356156

申请日：2023-07-20

Applicant: ProteinSimple

Inventor： Kevin TRAN ,  Jessica DERMODY ,  Joshua I. MOLHO ,  Surashree KULKARNI ,  Annegret BOGE

IPC: G01N27/447 ,  G01N1/40 ,  G01N21/64 ,  G01N33/68

CPC classification number: G01N27/44726 ,  G01N1/40 ,  G01N21/6486 ,  G01N33/6854 ,  G01N2001/4038 ,  G01N1/4044

Abstract: Some embodiments described herein relate to a method that includes wetting a microfluidic device with a sample containing biological cells. The chip can contain multiple wells. The sample can be allowed to “settle,” such that individual cells migrate into and/or are captured by various wells. Analytes from the cells can be electrophoretically separated. A voltage can be applied across the chip causing analytes to migrate from the wells and into the separation medium under electromotive force, where they can be separated based, for example, on their mobility. After separation, a reagent can be applied to the chip. The reagent can be configured to increase a probe's ability to bind to at least a subset of the analytes and facilitate detection and/or quantification of the analytes. The analytes can then be probed, for example, with an antibody.

公开(公告)号：US20210325377A1

公开(公告)日：2021-10-21

申请号：US16932441

申请日：2020-07-17

Applicant: ProteinSimple

Inventor： Irina Georgievna KAZAKOVA ,  Crystal TRAN ,  Jessica DERMODY ,  Annegret BOGE ,  Tom YANG

IPC: G01N33/536 ,  G01N33/533 ,  G01N33/535

Abstract: Some embodiments described herein relate to systems and methods operable to combine immunoassay and Total Protein techniques in a single sample run. Some embodiments described herein allow for multiple sequential immunoassays to be performed in the same microfluidic device. Some embodiments described herein relate to stripping reagents operable to remove primary antibodies associated with immunoassays. Such stripping reagents can allow for additional immunoassays and/or Total Protein assays to be performed on the same sample.

公开(公告)号：US20210325401A1

公开(公告)日：2021-10-21

申请号：US16932445

申请日：2020-07-17

Applicant: ProteinSimple

Inventor： Irina Georgievna KAZAKOVA ,  Crystal TRAN ,  Jessica DERMODY ,  Annegret BOGE ,  Tom YANG

IPC: G01N33/68 ,  G01N33/53

Abstract: Some embodiments described herein relate to systems and methods operable to combine immunoassay and Total Protein techniques in a single sample run. Some embodiments described herein allow for multiple sequential immunoassays to be performed in the same microfluidic device. Some embodiments described herein relate to stripping reagents operable to remove primary antibodies associated with immunoassays. Such stripping reagents can allow for additional immunoassays and/or Total Protein assays to be performed on the same sample.

公开(公告)号：US20220155284A1

公开(公告)日：2022-05-19

申请号：US17589350

申请日：2022-01-31

Applicant: ProteinSimple

Inventor： Irina Georgievna KAZAKOVA ,  Crystal TRAN ,  Jessica DERMODY ,  Annegret BOGE ,  Tom Weisan YANG

IPC: G01N33/536 ,  G01N33/533 ,  G01N33/535 ,  C07K1/26 ,  G01N33/53 ,  G01N33/559 ,  G01N33/68

Abstract: Some embodiments described herein relate to systems and methods operable to combine immunoassay and Total Protein techniques in a single sample run. Some embodiments described herein allow for multiple sequential immunoassays to be performed in the same microfluidic device. Some embodiments described herein relate to stripping reagents operable to remove primary antibodies associated with immunoassays. Such stripping reagents can allow for additional immunoassays and/or Total Protein assays to be performed on the same sample.