公开(公告)号：US20220260525A1

公开(公告)日：2022-08-18

申请号：US17560837

申请日：2021-12-23

Applicant: ProteinSimple

Inventor： Joshua I. MOLHO ,  Kelly GARDNER ,  Danh C. TRAN ,  Eric JABART ,  David NILSON ,  Yuri KOUCHNIR ,  Michael HUSTON ,  Colin B. KENNEDY ,  David John ROACH

IPC: G01N27/447

Abstract: A system for assaying a biological sample for a presence of a target analyte includes an assaying device and a computer controller. The assaying device includes a housing, a receptacle disposed in the housing, and a source of activation energy. The receptacle is configured to accept an electrophoresis cell. The electrophoresis cell has a recess area configured to accept a chip configured to accept the biological sample. The chip includes a polymeric separation medium with activatable functional groups that covalently bond to the target analyte when activated. The source of activation energy is configured to supply activation energy to activate the activatable functional groups. The computer controller is operably coupled to the source of activation energy and is configured to activate the source of activation energy to direct an application of activation energy to the polymeric separation medium to activate the activatable functional groups.

公开(公告)号：US20240210352A1

公开(公告)日：2024-06-27

申请号：US18241684

申请日：2023-09-01

Applicant: ProteinSimple

Inventor： Roland Y. WANG ,  Philip H. CHAO ,  Allen R. BORONKAY ,  David J. ROACH ,  Hui XU ,  Joshua I. MOLHO ,  Tom Weisan YANG

IPC: G01N27/447

CPC classification number: G01N27/44743 ,  G01N27/4473

Abstract: Some embodiments described herein relate to capillary-containing cartridges suitable for use with capillary electrophoresis instruments. Embodiments described herein generally relate to cartridges that include a transfer capillary coupled to a separation capillary. The transfer capillary can be configured to be disposed in sample reservoirs and/or buffer reservoirs. Suction applied through a sheath interface of the transfer capillary and the separation capillary can draw sample/buffer from such reservoirs and bring the sample/buffer into contact with the separation capillary. The separation capillary can be configured for separation of analytes contained within the sample, for example when an electric potential (i.e., voltage) is applied across the separation capillary.

公开(公告)号：US20240192164A1

公开(公告)日：2024-06-13

申请号：US18356156

申请日：2023-07-20

Applicant: ProteinSimple

Inventor： Kevin TRAN ,  Jessica DERMODY ,  Joshua I. MOLHO ,  Surashree KULKARNI ,  Annegret BOGE

IPC: G01N27/447 ,  G01N1/40 ,  G01N21/64 ,  G01N33/68

CPC classification number: G01N27/44726 ,  G01N1/40 ,  G01N21/6486 ,  G01N33/6854 ,  G01N2001/4038 ,  G01N1/4044

Abstract: Some embodiments described herein relate to a method that includes wetting a microfluidic device with a sample containing biological cells. The chip can contain multiple wells. The sample can be allowed to “settle,” such that individual cells migrate into and/or are captured by various wells. Analytes from the cells can be electrophoretically separated. A voltage can be applied across the chip causing analytes to migrate from the wells and into the separation medium under electromotive force, where they can be separated based, for example, on their mobility. After separation, a reagent can be applied to the chip. The reagent can be configured to increase a probe's ability to bind to at least a subset of the analytes and facilitate detection and/or quantification of the analytes. The analytes can then be probed, for example, with an antibody.