公开(公告)号：US20230062228A1

公开(公告)日：2023-03-02

申请号：US17870563

申请日：2022-07-21

Applicant: ProteinSimple

Inventor： Cheng ZHOU ,  Hui XU ,  Roland Y. WANG ,  David J. ROACH ,  Jessica DERMODY ,  Tom Weisan YANG ,  Jiaqi WU

IPC: B01L3/00

Abstract: Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.

公开(公告)号：US20250033043A1

公开(公告)日：2025-01-30

申请号：US18733288

申请日：2024-06-04

Applicant: ProteinSimple

Inventor： Cheng ZHOU ,  Hui XU ,  Roland Y. WANG ,  David J. ROACH ,  Jessica DERMODY ,  Tom Weisan YANG ,  Jiaqi WU

IPC: B01L3/00

Abstract: Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.

公开(公告)号：US20240310326A1

公开(公告)日：2024-09-19

申请号：US18415025

申请日：2024-01-17

Applicant: ProteinSimple

Inventor： Hui XU ,  Uyen NGUYEN

IPC: G01N27/447

CPC classification number: G01N27/44756 ,  G01N27/44791

Abstract: Embodiments described herein generally relate to cartridges suitable for performing electrophoretic separation of analytes. Cartridges described herein are particularly well suited for reuse. Cartridges described herein can include a reservoir disposed between a capillary and a container containing a run buffer. The reservoir can inhibit run buffer from intruding into the capillary, thereby allowing repeated electrophoretic separations to be more consistent, more accurate, and/or more reliable.

公开(公告)号：US20230272003A1

公开(公告)日：2023-08-31

申请号：US18301858

申请日：2023-04-17

Applicant: ProteinSimple

Inventor： Hui XU ,  Kenneth SWARTZ

IPC: C07K1/26 ,  G01N1/40 ,  G01N21/76 ,  G01N33/543 ,  G01N33/68 ,  C07K1/28 ,  G01N1/20 ,  G06V10/141 ,  G06V10/50 ,  G06V20/69

CPC classification number: C07K1/26 ,  G01N1/40 ,  G01N21/76 ,  G01N33/54366 ,  G01N33/6803 ,  C07K1/28 ,  G01N1/20 ,  G06V10/141 ,  G06V10/507 ,  G06V20/693 ,  G06V20/695 ,  G01N2001/4038 ,  G01N2021/1765

Abstract: Some embodiments described herein relate to a method that includes separating an analyte-containing sample via electrophoresis in a capillary. The capillary is loaded with a chemiluminescence agent, such as luminol, that is configured to react with the analyte (e.g., HRP-conjugated proteins) to produce a signal indicative of a concentration and/or quantity of analyte at each location along the length of the capillary. A first image of the capillary containing the analytes and the chemiluminescence agent is captured over a first period of time. A second image of the capillary containing the analytes and the chemiluminescence agent is captured over a second, longer, period of time. A concentration and/or quantity of a first population of analytes at a first location is determined using the first image, and a concentration and/or quantity of a second population of analytes at a second location is determined using the second image.

公开(公告)号：US20210356427A1

公开(公告)日：2021-11-18

申请号：US17385553

申请日：2021-07-26

Applicant: ProteinSimple

Inventor： Hui XU ,  Uyen NGUYEN

IPC: G01N27/447

Abstract: Embodiments described herein generally relate to cartridges suitable for performing electrophoretic separation of analytes. Cartridges described herein are particularly well suited for reuse. Cartridges described herein can include a reservoir disposed between a capillary and a container containing a run buffer. The reservoir can inhibit run buffer from intruding into the capillary, thereby allowing repeated electrophoretic separations to be more consistent, more accurate, and/or more reliable.

公开(公告)号：US20210231607A1

公开(公告)日：2021-07-29

申请号：US17063123

申请日：2020-10-05

Applicant: ProteinSimple

Inventor： David J. ROACH ,  Tom Weisan YANG ,  Hui XU

IPC: G01N27/447

Abstract: A system includes a housing, a cartridge retainer disposed within the housing, a detection assembly disposed within the housing, and a reagent tray holder movably disposed in the housing. The cartridge retainer configured to receive a capillary cartridge having a capillary. The detection assembly includes at least one emitter, a first detector, and a second detector. The detection assembly is configured to transition between a first configuration, in which the first detector detects a first output of the at least one emitter, and a second configuration, in which the second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of the capillary cartridge in fluid communication with a reagent volume.

公开(公告)号：US20240183820A1

公开(公告)日：2024-06-06

申请号：US18345798

申请日：2023-06-30

Applicant: ProteinSimple

Inventor： David J. ROACH ,  Tom Weisan YANG ,  Hui XU

IPC: G01N27/447 ,  G01N21/05 ,  G01N30/74

CPC classification number: G01N27/44791 ,  G01N27/447 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44747 ,  G01N21/05 ,  G01N27/44721 ,  G01N27/44726 ,  G01N27/44795 ,  G01N30/74

Abstract: A system includes a housing, a cartridge retainer disposed within the housing, a detection assembly disposed within the housing, and a reagent tray holder movably disposed in the housing. The cartridge retainer configured to receive a capillary cartridge having a capillary. The detection assembly includes at least one emitter, a first detector, and a second detector. The detection assembly is configured to transition between a first configuration, in which the first detector detects a first output of the at least one emitter, and a second configuration, in which the second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of the capillary cartridge in fluid communication with a reagent volume.

公开(公告)号：US20190285551A1

公开(公告)日：2019-09-19

申请号：US16423787

申请日：2019-05-28

Applicant: ProteinSimple

Inventor： Hui XU ,  Kenneth SWARTZ

IPC: G01N21/76 ,  G01N33/68 ,  G01N1/40 ,  G01N33/543

Abstract: Some embodiments described herein relate to a method that includes separating an analyte-containing sample via electrophoresis in a capillary. The capillary is loaded with a chemiluminescence agent, such as luminol, that is configured to react with the analyte (e.g., HRP-conjugated proteins) to produce a signal indicative of a concentration and/or quantity of analyte at each location along the length of the capillary. A first image of the capillary containing the analytes and the chemiluminescence agent is captured over a first period of time. A second image of the capillary containing the analytes and the chemiluminescence agent is captured over a second, longer, period of time. A concentration and/or quantity of a first population of analytes at a first location is determined using the first image, and a concentration and/or quantity of a second population of analytes at a second location is determined using the second image.

公开(公告)号：US20180321189A1

公开(公告)日：2018-11-08

申请号：US16033808

申请日：2018-07-12

Applicant: ProteinSimple

Inventor： David J. ROACH ,  Tom Weisan YANG ,  Hui XU

IPC: G01N27/447

Abstract: A system includes a housing, a cartridge retainer disposed within the housing, a detection assembly disposed within the housing, and a reagent tray holder movably disposed in the housing. The cartridge retainer configured to receive a capillary cartridge having a capillary. The detection assembly includes at least one emitter, a first detector, and a second detector. The detection assembly is configured to transition between a first configuration, in which the first detector detects a first output of the at least one emitter, and a second configuration, in which the second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of the capillary cartridge in fluid communication with a reagent volume.

公开(公告)号：US20240210352A1

公开(公告)日：2024-06-27

申请号：US18241684

申请日：2023-09-01

Applicant: ProteinSimple

Inventor： Roland Y. WANG ,  Philip H. CHAO ,  Allen R. BORONKAY ,  David J. ROACH ,  Hui XU ,  Joshua I. MOLHO ,  Tom Weisan YANG

IPC: G01N27/447

CPC classification number: G01N27/44743 ,  G01N27/4473

Abstract: Some embodiments described herein relate to capillary-containing cartridges suitable for use with capillary electrophoresis instruments. Embodiments described herein generally relate to cartridges that include a transfer capillary coupled to a separation capillary. The transfer capillary can be configured to be disposed in sample reservoirs and/or buffer reservoirs. Suction applied through a sheath interface of the transfer capillary and the separation capillary can draw sample/buffer from such reservoirs and bring the sample/buffer into contact with the separation capillary. The separation capillary can be configured for separation of analytes contained within the sample, for example when an electric potential (i.e., voltage) is applied across the separation capillary.