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公开(公告)号：US20230126263A1
公开(公告)日：2023-04-27
申请号：US18087772
申请日：2022-12-22
Applicant: ProteinSimple
Inventor： Jiaqi WU , Christopher HEGER
Abstract: Embodiments disclosed include systems, devices, and methods for analysis of samples containing particles used for gene delivery to determine a quality of the sample and/or an indication that the gene delivery particles are in a full, partial, and/or empty state. The present disclosure also relates to determining a protein and/or NA content in samples with known proportions of gene delivery particles in a full, partial, and/or empty state and based on the determination, establish a relationship between NA content and proportions of gene delivery particles in a full state. The present disclosure also relates to using such an established relationship to predict a proportion of the gene delivery particles in a full, partial, and/or empty state in test samples having the gene delivery particles in an unknown state.
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Patent Agency Ranking
公开(公告)号：US20250033043A1
公开(公告)日：2025-01-30
申请号：US18733288
申请日：2024-06-04
Applicant: ProteinSimple
Inventor： Cheng ZHOU , Hui XU , Roland Y. WANG , David J. ROACH , Jessica DERMODY , Tom Weisan YANG , Jiaqi WU
IPC: B01L3/00
Abstract: Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.
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3.发明申请公开(公告)号：US20180136115A1
公开(公告)日：2018-05-17
申请号：US15793434
申请日：2017-10-25
Applicant: ProteinSimple
Inventor： Jiaqi WU
CPC classification number: G01N21/25 , A61K47/68 , G01N21/31 , G01N33/6854 , G01N33/6857
Abstract: Provided herein are IEF focusing methods for determining the number of drug molecules present in at least one antibody-drug conjugate (ADC) species subpopulation. In one embodiment, the method comprises performing free solution isoelectric focusing on a sample comprising at least one ADC species, to obtain a focused sample. The absorbance of the sample at two different wavelengths is then measured, for example, over a range of pI values. Absorbance values at the two different wavelengths are compared at at least one corresponding pI value, where the at least one corresponding pI value is the pI of the ADC subpopulation. The number of drug molecules in the at least one ADC species subpopulation is then determined based on the comparison. The methods provided herein can also be used to determine the number of specific binding pair members bound to its target specific binding pair member.
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4.发明公开公开(公告)号：US20240052435A1
公开(公告)日：2024-02-15
申请号：US18493087
申请日：2023-10-24
Applicant: ProteinSimple
Inventor： Jiaqi WU , Christopher HEGER
Abstract: Embodiments disclosed include systems, devices, and methods for analysis of samples containing particles used for gene delivery to determine a quality of the sample and/or an indication that the gene delivery particles are in a full, partial, and/or empty state. The present disclosure also relates to determining a protein and/or NA content in samples with known proportions of gene delivery particles in a full, partial, and/or empty state and based on the determination, establish a relationship between NA content and proportions of gene delivery particles in a full state. The present disclosure also relates to using such an established relationship to predict a proportion of the gene delivery particles in a full, partial, and/or empty state in test samples having the gene delivery particles in an unknown state.
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公开(公告)号：US20230075533A1
公开(公告)日：2023-03-09
申请号：US17466916
申请日：2021-09-03
Applicant: ProteinSimple
Inventor： Jiaqi WU , Christopher HEGER
Abstract: Embodiments disclosed include systems, devices, and methods for analysis of samples containing particles used for gene delivery to determine a quality of the sample and/or an indication that the gene delivery particles are in a full, partial, and/or empty state. The present disclosure also relates to determining a protein and/or NA content in samples with known proportions of gene delivery particles in a full, partial, and/or empty state and based on the determination, establish a relationship between NA content and proportions of gene delivery particles in a full state. The present disclosure also relates to using such an established relationship to predict a proportion of the gene delivery particles in a full, partial, and/or empty state in test samples having the gene delivery particles in an unknown state.
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公开(公告)号：US20230062228A1
公开(公告)日：2023-03-02
申请号：US17870563
申请日：2022-07-21
Applicant: ProteinSimple
Inventor： Cheng ZHOU , Hui XU , Roland Y. WANG , David J. ROACH , Jessica DERMODY , Tom Weisan YANG , Jiaqi WU
IPC: B01L3/00
Abstract: Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.
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