公开(公告)号：US20230272003A1

公开(公告)日：2023-08-31

申请号：US18301858

申请日：2023-04-17

Applicant: ProteinSimple

Inventor： Hui XU ,  Kenneth SWARTZ

IPC: C07K1/26 ,  G01N1/40 ,  G01N21/76 ,  G01N33/543 ,  G01N33/68 ,  C07K1/28 ,  G01N1/20 ,  G06V10/141 ,  G06V10/50 ,  G06V20/69

CPC classification number: C07K1/26 ,  G01N1/40 ,  G01N21/76 ,  G01N33/54366 ,  G01N33/6803 ,  C07K1/28 ,  G01N1/20 ,  G06V10/141 ,  G06V10/507 ,  G06V20/693 ,  G06V20/695 ,  G01N2001/4038 ,  G01N2021/1765

Abstract: Some embodiments described herein relate to a method that includes separating an analyte-containing sample via electrophoresis in a capillary. The capillary is loaded with a chemiluminescence agent, such as luminol, that is configured to react with the analyte (e.g., HRP-conjugated proteins) to produce a signal indicative of a concentration and/or quantity of analyte at each location along the length of the capillary. A first image of the capillary containing the analytes and the chemiluminescence agent is captured over a first period of time. A second image of the capillary containing the analytes and the chemiluminescence agent is captured over a second, longer, period of time. A concentration and/or quantity of a first population of analytes at a first location is determined using the first image, and a concentration and/or quantity of a second population of analytes at a second location is determined using the second image.

公开(公告)号：US20190285551A1

公开(公告)日：2019-09-19

申请号：US16423787

申请日：2019-05-28

Applicant: ProteinSimple

Inventor： Hui XU ,  Kenneth SWARTZ

IPC: G01N21/76 ,  G01N33/68 ,  G01N1/40 ,  G01N33/543

Abstract: Some embodiments described herein relate to a method that includes separating an analyte-containing sample via electrophoresis in a capillary. The capillary is loaded with a chemiluminescence agent, such as luminol, that is configured to react with the analyte (e.g., HRP-conjugated proteins) to produce a signal indicative of a concentration and/or quantity of analyte at each location along the length of the capillary. A first image of the capillary containing the analytes and the chemiluminescence agent is captured over a first period of time. A second image of the capillary containing the analytes and the chemiluminescence agent is captured over a second, longer, period of time. A concentration and/or quantity of a first population of analytes at a first location is determined using the first image, and a concentration and/or quantity of a second population of analytes at a second location is determined using the second image.