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    Gel for isoelectric focusing
    2.
    发明授权
    Gel for isoelectric focusing 有权
    凝胶等电聚焦

    公开(公告)号：US08858771B2

    公开(公告)日：2014-10-14

    申请号：US13370362

    申请日：2012-02-10

    申请人： Bengt Bjellqvist ,  Kristina Uhlen

    发明人： Bengt Bjellqvist ,  Kristina Uhlen

    IPC分类号： G01N27/447 ,  C07K1/28

    CPC分类号： C07K1/28 ,  G01N27/44747 ,  G01N27/44795

    摘要： The present invention relates to the field of electrophoresis and more specifically to a gel or strip for separating peptide components by isoelectric focusing by producing IPG (immobilized pH gradient) gels or strips in a novel way before focusing. More closely, the peptides are focused in a novel IPG gel including an uneven or non-linear pH gradient having at least three separate stepwise arranged pH-intervals. After focusing the peptide resolution is high and the peptides are evenly distributed along the gel.

    摘要翻译： 本发明涉及电泳领域，更具体地涉及通过在聚焦之前以新颖的方式生产IPG（固定化pH梯度）凝胶或条带，通过等电聚焦分离肽组分的凝胶或条带。 更紧密地，肽聚焦在包含具有至少三个分开的逐步排列的pH间隔的不均匀或非线性pH梯度的新型IPG凝胶中。 聚焦后肽分辨率高，肽沿凝胶均匀分布。

    GEL FOR ISOELECTRIC FOCUSING
    4.
    发明申请
    GEL FOR ISOELECTRIC FOCUSING 有权
    凝胶聚焦

    公开(公告)号：US20120138464A1

    公开(公告)日：2012-06-07

    申请号：US13370362

    申请日：2012-02-10

    申请人： BENGT BJELLQVIST ,  KRISTINA UHLÉN

    发明人： BENGT BJELLQVIST ,  KRISTINA UHLÉN

    IPC分类号： C07K1/28

    CPC分类号： C07K1/28 ,  G01N27/44747 ,  G01N27/44795

    摘要： The present invention relates to the field of electrophoresis and more specifically to a gel or strip for separating peptide components by isoelectric focusing by producing IPG (immobilised pH gradient) gels or strips in a novel way before focusing. More closely, the peptides are focused in a novel IPG gel including an uneven or non-linear pH gradient having at least three separate stepwise arranged pH-intervals. After focusing the peptide resolution is high and the peptides are evenly distributed along the gel.

    摘要翻译： 本发明涉及电泳领域，更具体地涉及通过在聚焦之前以新颖的方式生产IPG（固定化pH梯度）凝胶或条带，通过等电聚焦分离肽组分的凝胶或条带。 更紧密地，肽聚焦在包含具有至少三个分开的逐步排列的pH间隔的不均匀或非线性pH梯度的新型IPG凝胶中。 聚焦后肽分辨率高，肽沿凝胶均匀分布。

    External gradient chromatofocusing
    5.
    发明授权
    External gradient chromatofocusing 失效
    外部梯度色谱聚焦

    公开(公告)号：US07790025B2

    公开(公告)日：2010-09-07

    申请号：US10556562

    申请日：2004-05-14

    申请人： Latchezar I. Tsonev ,  Allen G. Hirsh

    发明人： Latchezar I. Tsonev ,  Allen G. Hirsh

    IPC分类号： B01D15/08

    CPC分类号： B01D15/168 ,  B01D15/362 ,  B01D15/363 ,  B01D15/426 ,  C07K1/16 ,  C07K1/18 ,  C07K1/26 ,  C07K1/28 ,  G01N30/02

    摘要： The invention provides novel methods for the separation of charged molecules such as proteins according to the isoelectric points (pI's) and includes the systems and buffering compositions employed for isolating charged molecules. The invention further provides for modifications to the above described chromatographic methods that enable the separation of charged molecules exhibiting virtually identical pI's by shifting both the buffer's pKa and the pI's of the eluted charged molecules while they are traversing the ion exchange column.

    摘要翻译： 本发明提供了用于根据等电点（pI）分离带电分子如蛋白质的新方法，并且包括用于分离带电分子的系统和缓冲组合物。 本发明进一步提供对上述色谱方法的修改，其能够通过在洗脱的带电荷分子横过离子交换柱的同时移动缓冲液的pKa和洗脱的带电分子的pI来分离表现出实质上相同的pI的带电分子。

    Method and apparatus for separating biological molecules
    6.
    发明授权
    Method and apparatus for separating biological molecules 失效
    用于分离生物分子的方法和装置

    公开(公告)号：US06979390B2

    公开(公告)日：2005-12-27

    申请号：US10450498

    申请日：2001-10-23

    申请人： Giles Hugo William Sanders ,  John Hassard ,  Stuart Hassard ,  Dimitrios Sideris

    发明人： Giles Hugo William Sanders ,  John Hassard ,  Stuart Hassard ,  Dimitrios Sideris

    IPC分类号： C07K1/24 ,  C07K1/28 ,  G01N27/447 ,  G01N27/453

    CPC分类号： G01N27/44795 ,  C07K1/24 ,  C07K1/28 ,  G01N27/44773

    摘要： A system for separating biological molecules includes a chip (10) on which there is an electrophoresis separation microchannel (16). At the start of the channel there is an isoelectric focusing section (14) containing a gel having a pH gradient. Molecules to be separated are placed in the focusing section (14) and move to an equilibrium position under the influence of an electric field. The electrophoretic state of the molecules is then changed, and a high voltage potential applied across the entire length of the focusing and separation sections. The molecules migrate under the influence of the electric field from the focusing section into the separation section where their position and velocity are tracked. From a knowledge of position and velocity, a user can determine both charge/mass ratio and isoelectric equilibrium point for each molecule. The gels can be replicated in the chip to allow parallel analysis, and hence comparisons, to take place.

    摘要翻译： 用于分离生物分子的系统包括其上存在电泳分离微通道（16）的芯片（10）。 在通道的开始处，存在含有具有pH梯度的凝胶的等电聚焦部分（14）。 要分离的分子被放置在聚焦部分（14）中并在电场的影响下移动到平衡位置。 然后改变分子的电泳状态，并且在聚焦和分离部分的整个长度上施加高电压电位。 分子在电场的影响下从聚焦部分迁移到其位置和速度被跟踪的分离部分。 根据位置和速度的知识，用户可以确定每个分子的电荷/质量比和等电点平衡点。 凝胶可以在芯片中复制，以便进行并行分析，从而进行比较。

    Tandem isotachophoresis/zone electrophoresis method and system
    8.
    发明申请
    Tandem isotachophoresis/zone electrophoresis method and system 有权
    串联等电泳/区域电泳方法和系统

    公开(公告)号：US20020079223A1

    公开(公告)日：2002-06-27

    申请号：US09933993

    申请日：2001-08-20

    发明人： Stephen J. Williams ,  Hong Dong Tan ,  Hung Pin Kao ,  Wyatt N. Vreeland

    IPC分类号： G01N027/26 ,  G01N027/447

    CPC分类号： B01L3/5027 ,  B01L3/502753 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/049 ,  C07K1/26 ,  C07K1/28 ,  G01N27/44773

    摘要： A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte. With the application of a voltage potential across the microchannel, charged components in the sample stack by isotachophoresis, and electrolytic hydroxyl or hydrogen ions formed by electrolysis at the upstream-end electrode migrate into the trailing-edge ion buffer, titrating the titratable species therein, where the concentration of the titratable species in the trailing-edge electrolyte is selected, in relation to the lengths of the upstream channel region and sample-loading volume, to permit the sample to stack into a relatively small sample volume before electrolytic-ion migration from the upstream electrode into and through the sample-volume region is effective to overtake the charged sample components. With continued application of an electric potential across the channel ends, charged sample components in the stacked sample volume separate by zone electrophoresis.

    摘要翻译： 公开了一种分离具有给定负电荷并包含在样品中的组分的方法。 在一个实施方案中，该方法包括将具有样品的微通道加载到放置在具有选定浓度的可滴定物质的后缘电解质和前沿电解质之间。 通过在微通道上施加电压电位，通过等速电泳施加样品叠层中的带电组分，并且通过在上游端电极处电解形成的电解羟基或氢离子迁移到后缘离子缓冲液中，滴定其中的可滴定物质， 其中选择后缘电解质中的可滴定物质的浓度，相对于上游通道区域的长度和样品加载体积，以允许样品在电离离子迁移之前堆积成相对小的样品体积 进入和通过样品体积区域的上游电极有效地超过带电的样品组分。 通过在通道末端继续施加电位，通过区域电泳分离堆叠样品体积中的带电样品组分。

    Proton concentration topographies, methods and devices for producing the same
    10.
    发明授权
    Proton concentration topographies, methods and devices for producing the same 有权
    质子浓度分布图，生产方法和装置

    公开(公告)号：US09028664B2

    公开(公告)日：2015-05-12

    申请号：US13390352

    申请日：2010-08-18

    申请人： Uri Sivan ,  Elad Brod

    发明人： Uri Sivan ,  Elad Brod

    IPC分类号： G01N27/447 ,  B01D57/02 ,  B01D61/44 ,  C07K1/28 ,  G01N21/80

    CPC分类号： G01N27/44795 ,  B01D57/02 ,  B01D61/445 ,  C07K1/28 ,  G01N21/80 ,  G01N27/44747 ,  G01N27/44773

    摘要： A device for isoelectric focusing. The device comprises a focusing container configured to contain an electrolyte solution and having a longitudinal axis and at least one electrolysis unit mounted in a close proximity to the longitudinal axis. Each electrolysis unit injects an ion flow into the focusing container so as to create a pH gradient having a plurality of steps in the electrolyte solution, along the longitudinal axis. Each step has a substantially uniform pH level and the pH gradient is defined by at least one pH ramp between every two sequential steps of the plurality of steps.

    摘要翻译： 等电聚焦装置。 该装置包括配置成容纳电解质溶液并具有纵向轴线的至少一个电解单元的聚焦容器，并且至少一个电解单元安装在靠近纵向轴线的位置。 每个电解单元将离子流注入到聚焦容器中，以沿着纵向轴线产生在电解液中具有多个步骤的pH梯度。 每个步骤具有基本上均匀的pH水平，并且pH梯度由多个步骤的每两个连续步骤之间的至少一个pH斜坡限定。