公开(公告)号：US20220260525A1

公开(公告)日：2022-08-18

申请号：US17560837

申请日：2021-12-23

Applicant: ProteinSimple

Inventor： Joshua I. MOLHO ,  Kelly GARDNER ,  Danh C. TRAN ,  Eric JABART ,  David NILSON ,  Yuri KOUCHNIR ,  Michael HUSTON ,  Colin B. KENNEDY ,  David John ROACH

IPC: G01N27/447

Abstract: A system for assaying a biological sample for a presence of a target analyte includes an assaying device and a computer controller. The assaying device includes a housing, a receptacle disposed in the housing, and a source of activation energy. The receptacle is configured to accept an electrophoresis cell. The electrophoresis cell has a recess area configured to accept a chip configured to accept the biological sample. The chip includes a polymeric separation medium with activatable functional groups that covalently bond to the target analyte when activated. The source of activation energy is configured to supply activation energy to activate the activatable functional groups. The computer controller is operably coupled to the source of activation energy and is configured to activate the source of activation energy to direct an application of activation energy to the polymeric separation medium to activate the activatable functional groups.

公开(公告)号：US20240264115A1

公开(公告)日：2024-08-08

申请号：US18440572

申请日：2024-02-13

Applicant: ProteinSimple

Inventor： Tom W. YANG ,  David John ROACH

IPC: G01N27/447 ,  C07K1/26 ,  C07K1/28

CPC classification number: G01N27/44782 ,  G01N27/44704 ,  C07K1/26 ,  C07K1/285 ,  G01N27/44726 ,  G01N27/44791

Abstract: An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided.

公开(公告)号：US20220236221A1

公开(公告)日：2022-07-28

申请号：US17718037

申请日：2022-04-11

Applicant: ProteinSimple

Inventor： David John ROACH ,  Hui XU ,  Tom Weisan YANG

IPC: G01N27/447 ,  B01D57/02

Abstract: Embodiments described herein relate to single-channel free-flow electrophoresis devices or apparatuses, and methods for separating and collecting analytes of interest from a sample by sequentially adjusting the pH value of the electrolyte buffers and separating the analyte of interest according to the corresponding isoelectric points of the analyte of interest. The method includes flowing a sample through a single center channel, applying an electric field perpendicular to a flow direction of the sample via an anolyte channel and a catholyte channel that are parallel to the center channel, and then collecting a fraction of the analyte of interest in accordance with their respective isoelectric points.

公开(公告)号：US20180106758A1

公开(公告)日：2018-04-19

申请号：US15707600

申请日：2017-09-18

Applicant: ProteinSimple

Inventor： Tom W. YANG ,  David John ROACH

IPC: G01N27/447 ,  C07K1/26 ,  C07K1/28

CPC classification number: G01N27/44782 ,  C07K1/26 ,  C07K1/285 ,  G01N27/44704 ,  G01N27/44726 ,  G01N27/44791

Abstract: An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided.