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    LABELLED COMPOUNDS AND METHODS FOR MASS SPECTROMETRY-BASED QUANTIFICATION
    1.
    发明申请
    LABELLED COMPOUNDS AND METHODS FOR MASS SPECTROMETRY-BASED QUANTIFICATION 审中-公开

    公开(公告)号：US20190187152A1

    公开(公告)日：2019-06-20

    申请号：US16327182

    申请日：2017-08-17

    Applicant: BiognoSYS AG

    Inventor： Lukas REITER ,  Susanne Leslie MÜLLER

    IPC: G01N33/68

    CPC classification number: G01N33/6848 ,  G01N33/6842 ,  G01N2440/20 ,  G01N2440/38 ,  G01N2560/00

    Abstract: Methods for peptide and/or protein quantification by mass spectrometry using labeled peptides, wherein multiple labels lead to distinct fragments for the labeled peptides and their unlabeled variant, thus facilitating data analysis and enhancing the potential for quantification. Methods for selecting the label and label position are further given, as well as sets of labeled peptides resulting from or for use in the above-mentioned methods. The methods and substances are especially useful for data-independent or multiplexed parallel reaction monitoring proteomics applications involving peptide quantification.

    ANTIBODY MANUFACTURE
    3.
    发明公开
    ANTIBODY MANUFACTURE 审中-公开

    公开(公告)号：US20230221334A1

    公开(公告)日：2023-07-13

    申请号：US18154386

    申请日：2023-01-13

    Applicant: BAYER HEALTHCARE LLC

    Inventor： Hendri TJANDRA ,  Yi LIU ,  Vu THAI

    IPC: G01N33/68 ,  C07K1/34

    CPC classification number: G01N33/6854 ,  C07K1/34 ,  G01N2440/20

    Abstract: Described herein is a method for assessing disulfide bond reduction potential of a protein of interest comprising the following steps:



    Providing a cell culture fluid sample comprising mammalian cells expressing a protein of interest at a concentration within the range of between 0.2 g/l to 10 g/l
    Filtering said cell culture fluid sample over at least one filter
    Displacing O2 in the filtered cell culture fluid sample
    Collecting at least one sample of the O2-displaced filtered cell culture fluid sample
    Separating the proteins in said at least one O2-displaced, filtered cell culture fluid sample according to their size under non-denaturing conditions
    Determining the disulfide bond reduction potential of the protein of interest.

    COMPOSITIONS AND METHODS FOR ANALYSIS OF PROTEIN SEQUENCES AND POST-TRANSLATIONAL MODIFICATIONS
    5.
    发明申请
    COMPOSITIONS AND METHODS FOR ANALYSIS OF PROTEIN SEQUENCES AND POST-TRANSLATIONAL MODIFICATIONS 审中-公开
    蛋白质序列分析和转录后修饰的组合物和方法

    公开(公告)号：US20160349269A1

    公开(公告)日：2016-12-01

    申请号：US15116609

    申请日：2015-02-04

    Applicant: Donald F. HUNT ,  Welhan WANG ,  Lichao ZHANG ,  UNIVERSITY OF VIRGINIA PATENT FOUNDATION

    Inventor： Donald F. Hunt ,  Weihan Wang ,  Lichao Zhang

    IPC: G01N33/68

    CPC classification number: G01N33/6848 ,  G01N33/6854 ,  G01N2440/00 ,  G01N2440/16 ,  G01N2440/20 ,  G01N2440/38 ,  G01N2560/00

    Abstract: The application discloses, compositions, methods, systems, and apparatuses for rapid sequence analysis of proteins, including location of post-translational modifications and disulfide bonds, is described. Limited digestion of fully denatured antibody occurs in seconds by flowing sample in 8 M urea at constant pressure through a micro column reactor containing immobilized aspergillopepsin I, resulting in a product mixture containing 3-10 kDa peptides, which is then fractionated by capillary column chromatography and analyzed by both electron transfer dissociation (ETD) and collision activated dissociation mass spectrometry. This method provides 95% sequence coverage of a mAb and detects numerous post-translational modifications. For disulfide bond location, native mAb is subjected to longer digestion times. Release of disulfide containing peptides from accessible regions of the folded protein occurs with short digestion times. The identity of peptides connected by a disulfide bond is determined using ETD and ion-ion proton transfer chemistry.

    Abstract translation: 本申请公开了用于蛋白质快速序列分析的组合物，方法，系统和装置，包括翻译后修饰和二硫键的位置。 完全变性抗体的有限消化在几秒钟内通过在8M尿素恒定压力下通过含有固定化曲霉素I的微柱反应器流动样品，产生含有3-10kDa肽的产物混合物，然后通过毛细管柱色谱法分级分离， 通过电子转移解离（ETD）和碰撞激活解离质谱分析。 该方法提供了mAb的95％序列覆盖率并检测了许多翻译后修饰。 对于二硫键位置，天然mAb经受较长的消化时间。 在折叠的蛋白质的可接近区域释放含二硫键的肽发生短消化时间。 通过二硫键连接的肽的身份使用ETD和离子离子质子转移化学测定。

    SYSTEMS AND METHODS FOR THE DETECTION AND ANALYSIS OF FREE THIOL
    9.
    发明公开
    SYSTEMS AND METHODS FOR THE DETECTION AND ANALYSIS OF FREE THIOL 审中-公开

    公开(公告)号：US20240344982A1

    公开(公告)日：2024-10-17

    申请号：US18604002

    申请日：2024-03-13

    Applicant: ProteinSimple

    Inventor： James MCELROY ,  Christopher HEGER

    IPC: G01N21/64 ,  G01N33/58

    CPC classification number: G01N21/6428 ,  G01N33/582 ,  G01N2440/20 ,  G01N2500/00

    Abstract: Embodiments described herein relate to devices, and methods for quantifying thiol content in a sample containing a mixture of proteins or protein isoforms. The method includes conjugating a portion of the sample with free thiol detection binders, separating the contents in the portion of the sample into separated protein isoforms, detecting fluorescence signals associated with each separated protein isoform, and quantifying, based on the fluorescence signals, a relative amount of free thiol associated with each separated protein isoform. In some instances, the method includes quantifying the amount of each separated protein isoform based on absorbance signals associated with each separated protein isoform. In some instances, the fluorescence and/or absorbance signals associated with protein isoforms conjugated with detection binders can be compared with the corresponding signals associated with unconjugated protein isoforms. In some instances, the method further includes applying a reducing agent and quantifying total-thiol content in the sample.

    Labelled compounds and methods for mass spectrometry-based quantification
    10.
    发明授权
    Labelled compounds and methods for mass spectrometry-based quantification 有权

    公开(公告)号：US11754571B2

    公开(公告)日：2023-09-12

    申请号：US17681223

    申请日：2022-02-25

    Applicant: BiognoSYS AG

    Inventor： Lukas Reiter ,  Susanne Leslie Müller

    IPC: G01N33/68

    CPC classification number: G01N33/6848 ,  G01N33/6842 ,  G01N2440/20 ,  G01N2440/38 ,  G01N2560/00

    Abstract: Methods for peptide and/or protein quantification by mass spectrometry using labeled peptides, wherein multiple labels lead to distinct fragments for the labeled peptides and their unlabeled variant, thus facilitating data analysis and enhancing the potential for quantification. Methods for selecting the label and label position are further given, as well as sets of labeled peptides resulting from or for use in the above-mentioned methods. The methods and substances are especially useful for data-independent or multiplexed parallel reaction monitoring proteomics applications involving peptide quantification.

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