公开(公告)号：US11726043B2

公开(公告)日：2023-08-15

申请号：US17199934

申请日：2021-03-12

Applicant: University of Idaho

Inventor： Andreas E. Vasdekis ,  Nava Subedi ,  Shahla Nemati

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/02 ,  G02B21/26 ,  H04N23/56 ,  H04N23/90

CPC classification number: G01N21/6486 ,  G01N21/6458 ,  G02B21/008 ,  G02B21/0048 ,  G02B21/0056 ,  G02B21/0064 ,  G02B21/0076 ,  G02B21/0088 ,  G02B21/025 ,  G02B21/26 ,  H04N23/56 ,  H04N23/90 ,  G01N2021/6471 ,  G01N2201/0675

Abstract: Certain disclosed embodiments concern an integrated imaging system that combined light-sheet microscopy, which enables considerable speed and phototoxicity gains, with quantitative-phase imaging. A method for using such imaging systems also is disclosed. In an exemplary embodiment, an integrated imaging system was used for multivariate investigation of live-cells in microfluidics.

公开(公告)号：US20190240665A1

公开(公告)日：2019-08-08

申请号：US16160816

申请日：2018-10-15

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. LIONBERGER ,  Matthew E. FOWLER ,  Phillip J. M. ELMS ,  Kevin D. LOUTHERBACK ,  Randall D. LOWE, JR. ,  Jian GONG ,  Tanner J. NEVILL ,  Gang F. WANG ,  Gregory G. LAVIEU ,  John A. TENNEY ,  Aathavan KARUNAKARAN

IPC: B01L3/00 ,  G06T7/00 ,  B01L9/00

CPC classification number: B01L3/502761 ,  B01L9/527 ,  B01L2300/0829 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N21/05 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2201/0675 ,  G06T7/0014

Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

公开(公告)号：US20180321479A1

公开(公告)日：2018-11-08

申请号：US15843692

申请日：2017-12-15

Applicant: SCREEN HOLDINGS CO., LTD. ,  The Board of Trustees of the Leland Stanford Junior University

Inventor： Joseph Russell Landry ,  Ryosuke Itoh ,  Michael J. Mandella ,  Olav Solgaard

IPC: G02B21/00 ,  G02B21/36 ,  G01N21/64

CPC classification number: G02B21/0088 ,  G01N21/6458 ,  G01N2201/0675 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/365 ,  G02B21/367

Abstract: A system, including a structured illumination stage to provide a spatially modulated imaging field is provided. The system further includes a spatial frequency modulation stage to adjust the frequency of the spatially modulated imaging field, a sample interface stage to direct the spatially modulated imaging field to a sample, and a sensor configured to receive a plurality of fluorescence emission signals from the sample. The system also includes a processor configured to reduce a sample scattering signal and to provide a fluorescence emission signal from a portion of the sample including the spatially modulated imaging field. A method for using the above system to form an image of the sample is also provided.

公开(公告)号：US10036735B2

公开(公告)日：2018-07-31

申请号：US14913958

申请日：2014-08-26

Applicant: The Regents of The University of Colorado, A Body Corporate

Inventor： Rafael Piestun ,  Hengyi Ju ,  Jacob Dove ,  Antonio Miguel Caravaca-Aguirre ,  Todd Murray ,  Donald Conkey

IPC: G01N29/44 ,  G01N21/00 ,  G01N29/24 ,  A61B5/00 ,  G01N21/17 ,  G01N29/06

CPC classification number: G01N29/2418 ,  A61B5/0095 ,  G01N21/1702 ,  G01N29/0654 ,  G01N2021/1706 ,  G01N2201/0675

Abstract: Systems and methods are disclosed to enhance three-dimensional photoacoustic imaging behind, through, or inside a scattering material. Some embodiments can increase the optical fluence in an ultrasound transducer focus and/or enhance the optical intensity using wavefront shaping before the scatterer. The photoacoustic signal induced by an object placed behind the scattering medium can serve as feedback to optimize the wavefront, enabling one order of magnitude enhancement of the photoacoustic amplitude. Using the enhanced optical intensity, the object can be scanned in two dimensions and/or a spot can be scanned by re-optimizing the wavefront before post-processing of the data to reconstruct the image. The temporal photoacoustic signal provides information to reconstruct the third-dimensional information.

公开(公告)号：US09671341B2

公开(公告)日：2017-06-06

申请号：US14795491

申请日：2015-07-09

Applicant: Carl Zeiss Microscopy GmbH

Inventor： Martin Müller ,  Nico Presser ,  Gunter Möhler

IPC: G01N21/64 ,  G02B21/00

CPC classification number: G01N21/6408 ,  G01N21/6458 ,  G01N2201/06113 ,  G01N2201/067 ,  G01N2201/0675 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/008

Abstract: Method for the operation of a laser scanning microscope. The microscope includes an illumination beam path in which at least one illumination light source is arranged, a detection beam path in which at least one photomultiplier (PMT) is incorporated as detector, and a control unit for controlling fluorescence experiments. A sample is alternately illuminated at high intensity via the control unit, and the fluorescence decay behavior of sample points and/or sample regions is subsequently detected. The PMT is switched on and off depending on the illumination mode by the control unit via a switch directly in the high-voltage supply of the PMT.

公开(公告)号：US20170074796A1

公开(公告)日：2017-03-16

申请号：US15311311

申请日：2015-05-08

Applicant: TOHOKU UNIVERSITY ,  NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY

Inventor： Akihide HIBARA ,  Manabu TOKESHI ,  Osamu WAKAO

IPC: G01N21/64

CPC classification number: G01N21/6445 ,  G01J3/0224 ,  G01J4/00 ,  G01N21/64 ,  G01N21/6456 ,  G01N33/542 ,  G01N2201/0675 ,  G01N2201/0683 ,  G02F1/13

Abstract: An excitation light source emits excitation light to a target sample. An image sensor includes pixels arranged one-dimensionally or two-dimensionally, and receives measurement light from the sample according to the excitation light. A polarization selector arranged between the sample and image sensor includes pixels arranged one-dimensionally or two-dimensionally. Each pixel receives a corresponding portion of the measurement light, selects light having a polarization direction that corresponds to a driving signal applied to the pixels, and supplies this light to the image sensor. A measurement control unit supplies the cyclic driving signal having a first period T1 and acquires data I1, I2, I3, and I4 from each pixel of the image sensor for each exposure time segment T2=T1/4 obtained by dividing the first period T1 by 4.

Abstract translation: 激发光源向目标样品发射激发光。 图像传感器包括一维或二维排列的像素，并且根据激发光接收来自样品的测量光。 布置在样本和图像传感器之间的偏振选择器包括一维或二维布置的像素。 每个像素接收测量光的对应部分，选择具有与施加到像素的驱动信号相对应的偏振方向的光，并将该光提供给图像传感器。 测量控制单元提供具有第一周期T1的循环驱动信号，并且通过将每个曝光时间段T2 = T1 / 4从图像传感器的每个像素获取数据I1，I2，I3和I4， 4。

公开(公告)号：US09488568B2

公开(公告)日：2016-11-08

申请号：US14568122

申请日：2014-12-12

Applicant: Otsuka Electronics Co., Ltd.

Inventor： Kazuhiro Sugita ,  Yusuke Yamazaki ,  Haruka Otsuka

IPC: G01N21/21

CPC classification number: G01N21/211 ,  G01N2021/213 ,  G01N2201/0675

Abstract: Provided is a polarization analysis apparatus that can quickly measure the polarization properties of a sample. The polarization analysis apparatus includes a light source configured to emit light in a predetermined wavelength region, a polarizer configured to transmit the light emitted from the light source, a spatial phase modulator configured to transmit the light from the sample, an analyzer configured to transmit the light that has passed through the spatial phase modulator, and an imaging spectrometer configured to receive the light that has passed through the analyzer. The spatial phase modulator is formed of a birefringent material, and is configured to have different phase differences at respective positions in a first direction in a plane orthogonal to an optical axis. The imaging spectrometer disperses the received light in a second direction that is different from the first direction in the plane orthogonal to the optical axis.

Abstract translation: 提供了一种可以快速测量样品的偏振特性的偏振分析装置。 所述偏振光分析装置包括被配置为发射预定波长区域的光的光源，被配置为透射从所述光源发射的光的偏振器，被配置为透射来自所述样品的光的空间相位调制器， 已经通过空间相位调制器的光和被配置为接收已经通过分析器的光的成像光谱仪。 空间相位调制器由双折射材料形成，并且被配置为在与光轴正交的平面中的第一方向上的各个位置处具有不同的相位差。 成像光谱仪将接收的光在与光轴正交的平面中与第一方向不同的第二方向分散。

公开(公告)号：US09476824B2

公开(公告)日：2016-10-25

申请号：US14054149

申请日：2013-10-15

Applicant: Purdue Research Foundation

Inventor： Dor Ben-Amotz ,  Bradley J. Lucier ,  Gregery T. Buzzard ,  David Wilcox ,  Ping Wang ,  Bharat R. Mankani

IPC: G01N21/25 ,  G01N21/65 ,  G01J3/44

CPC classification number: G01N21/25 ,  G01J3/44 ,  G01N21/65 ,  G01N2201/0675 ,  G01N2201/129

Abstract: A method for measuring a sample to identify a chemical includes receiving respective spectra for each of a plurality of chemicals. Using a processor, a plurality of binary mathematical filters are computed using the received spectra. A spatial light modulator is adjusted according to a selected mathematical filter. Light that has interacted with the sample is dispersed over the surface of the spatial light modulator, so that the spatial light modulator provides light at wavelengths corresponding to the selected mathematical filter. The light provided by the spatial light modulator is measured to provide a score corresponding to the selected mathematical filter. Filter scores are combined to determine a chemical amount. The processor can operate detection apparatus having a light source, an objective for focusing source light onto the sample, a spatial light modulator, and a detector for detecting the modulator output.

Abstract translation: 用于测量样品以鉴定化学品的方法包括接收多种化学品中的每一种的相应光谱。 使用处理器，使用接收的光谱来计算多个二进制数学滤波器。 根据所选择的数学滤波器调整空间光调制器。 已经与样品相互作用的光分散在空间光调制器的表面上，使得空间光调制器提供对应于所选择的数学滤波器的波长的光。 测量由空间光调制器提供的光以提供对应于所选数学滤波器的分数。 过滤分数结合以确定化学量。 处理器可以操作具有光源的检测装置，用于将源光聚焦到样本上的物镜，空间光调制器和用于检测调制器输出的检测器。

公开(公告)号：US20160282594A1

公开(公告)日：2016-09-29

申请号：US15180301

申请日：2016-06-13

Applicant: Verily Life Sciences LLC

Inventor： Victor Marcel Acosta ,  Adam Backer ,  John D. Perreault ,  Daniele Paolo Piponi

IPC: G02B21/00 ,  H04N5/33 ,  G06T7/40 ,  G02F1/23 ,  G02B21/06 ,  G02B21/36 ,  G01N21/27 ,  H04N5/225 ,  G06T7/00

CPC classification number: G02B21/0064 ,  G01J3/0205 ,  G01J3/021 ,  G01J3/0229 ,  G01J3/0237 ,  G01J3/18 ,  G01J3/502 ,  G01J2003/1278 ,  G01J2003/468 ,  G01N21/255 ,  G01N21/27 ,  G01N2201/06113 ,  G01N2201/0675 ,  G02B5/1828 ,  G02B6/2931 ,  G02B21/06 ,  G02B21/361 ,  G02B26/0808 ,  G02F1/133553 ,  G02F1/23 ,  G02F1/292 ,  G06T7/11 ,  G06T7/90 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30004 ,  H04N5/2256 ,  H04N5/332

Abstract: An imaging system includes a light source configured to illuminate a target and a camera configured to image light responsively emitted from the target and reflected from a spatial light modulator (SLM). The imaging system is configured to generate high-resolution, hyperspectral images of the target. The SLM includes a refractive layer that is chromatically dispersive and that has a refractive index that is controllable. The refractive index of the refractive layer can be controlled to vary according to a gradient such that light reflected from the SLM is chromatically dispersed and spectrographic information about the target can be captured using the camera. Such a system could be operated confocally, e.g., by incorporating a micromirror device configured to control a spatial pattern of illumination of the target and to modulate the transmission of light from the target to the camera via the SLM according to a corresponding spatial pattern.

Abstract translation: 成像系统包括被配置为照射目标的光源和被配置为对从目标发射并从空间光调制器（SLM）反射的）反射的图像的照相机。 成像系统被配置为产生目标的高分辨率，高光谱图像。 SLM包括色度分散且具有可控制的折射率的折射层。 折射层的折射率可以根据梯度来变化，使得从SLM反射的光被色散，并且可以使用照相机捕获关于目标的光谱信息。 这样的系统可以协调地操作，例如通过结合配置成控制目标的照明的空间模式的微镜装置，并且根据对应的空间模式经由SLM调制从目标到照相机的光的传输。

公开(公告)号：US09360428B2

公开(公告)日：2016-06-07

申请号：US14769893

申请日：2014-03-14

Applicant: The Regents of the University of California

Inventor： Xiaodong Tao ,  Joel A. Kubby

IPC: G01N21/45 ,  G01N21/64 ,  G01N21/49 ,  G02B21/16 ,  G02B21/24 ,  G02B21/36 ,  G01N21/47

CPC classification number: G02B27/0068 ,  G01J9/00 ,  G01N21/45 ,  G01N21/49 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/4709 ,  G01N2021/6439 ,  G01N2201/06113 ,  G01N2201/0675 ,  G01N2201/121 ,  G02B21/16 ,  G02B21/244 ,  G02B21/245 ,  G02B21/361 ,  G02B21/365 ,  G02B27/0927

Abstract: Interferometric focusing (IF), rather than conventional geometric focusing, of excitation light onto a guide-star that is embedded deeply in tissue, increases its fluorescence intensity. The method can extend the depth of wavefront measurement and improve correction inside of tissues because of its ability to suppress both scattering of diffuse light and aberration of ballistic light. The results showed more than two times improvement in SNR and RMS error of the wavefront measurement. Although only ballistic light in the excitation path is corrected, the intensity after wavefront correction increased by 1.5 times. When applying IF to a two-photon microscope with a near infra-red laser, this method would further extend the measurement depth and achieve high SNR for the wavefront sensor.