公开(公告)号：US10518196B2

公开(公告)日：2019-12-31

申请号：US15209411

申请日：2016-07-13

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Christopher Michael Puleo ,  Craig Patrick Galligan ,  Gregory Andrew Grossman ,  Erik Leeming Kvam ,  Robert Scott Duthie ,  Kenneth Wayne Rigby ,  Paul Michael Smigelski, Jr. ,  Victoria Eugenia Cotero ,  Jason William Castle ,  John Donald Burczak ,  James Edward Rothman

IPC: B01D21/00 ,  B01D21/24 ,  G01N33/49 ,  B01L3/00 ,  G01N1/40 ,  C12M1/12 ,  C12M1/26 ,  B01D17/02 ,  B01D21/02 ,  C12M1/00 ,  C02F1/48 ,  C02F1/44 ,  C02F101/32

Abstract: A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.

公开(公告)号：US20180273914A1

公开(公告)日：2018-09-27

申请号：US16005633

申请日：2018-06-11

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Erik Leeming Kvam ,  Robert Scott Duthie ,  John Richard Nelson

IPC: C12N7/00 ,  C12Q1/70 ,  C12Q1/6806 ,  B01L3/00 ,  C12N15/10

CPC classification number: C12N7/00 ,  B01L3/508 ,  B01L2200/082 ,  C12N15/1017 ,  C12N2760/14111 ,  C12N2760/14151 ,  C12N2760/14163 ,  C12Q1/6806 ,  C12Q1/70 ,  C12Q1/701 ,  C12Q2523/113 ,  C12Q2523/308 ,  C12Q2527/125

Abstract: The present disclosure generally relates to a method and device for inactivation and dry storage, under ambient conditions, of a biological sample containing RNA virus. Methods for collecting and recovering RNA from a biological sample and subsequent analysis for a virus are also provided.

公开(公告)号：US20170145387A1

公开(公告)日：2017-05-25

申请号：US14945483

申请日：2015-11-19

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Erik Leeming Kvam ,  Robert Scott Duthie ,  John Richard Nelson

IPC: C12N7/00 ,  B01L3/00 ,  C12Q1/70

CPC classification number: C12N7/00 ,  B01L3/508 ,  B01L2200/082 ,  C12N15/1017 ,  C12N2760/14111 ,  C12N2760/14151 ,  C12N2760/14163 ,  C12Q1/6806 ,  C12Q1/70 ,  C12Q1/701 ,  C12Q2523/113 ,  C12Q2523/308 ,  C12Q2527/125

Abstract: The present disclosure generally relates to a method and device for inactivation and dry storage, under ambient conditions, of a biological sample containing RNA virus. Methods for collecting and recovering RNA from a biological sample and subsequent analysis for a virus are also provided.

公开(公告)号：US09593368B2

公开(公告)日：2017-03-14

申请号：US14321235

申请日：2014-07-01

Applicant: General Electric Company

Inventor： John Richard Nelson ,  Robert Scott Duthie ,  Christopher Michael Puleo ,  Patrick McCoy Spooner

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6848 ,  C12Q2531/119 ,  C12Q2531/125 ,  C12Q2565/518

Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; applying an aqueous buffer to the sample application zone of the substrate to washes away one or more inhibitors present on the sample application zone; and applying an isothermal nucleic acid amplification reaction mixture to the sample application zone to amplify the target nucleic acid to form a nucleic acid amplification product. The target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight.

Abstract translation: 本文提供了一种方法，所述方法包括：将包含靶核酸的样品施加到底物的样品施用区; 将水性缓冲液施加到基材的样品施加区域以洗涤存在于样品施加区上的一种或多种抑制剂; 以及将等温核酸扩增反应混合物应用于样品施用区域以扩增靶核酸以形成核酸扩增产物。 具有第一分子量的靶核酸基本上固定在样品施用区，并且其中扩增产物具有第二分子量。

公开(公告)号：US20140255272A1

公开(公告)日：2014-09-11

申请号：US14285099

申请日：2014-05-22

Applicant: General Electric Company

Inventor： Robert Scott Duthie ,  Wei-Cheng Tian ,  Tarun Khurana

IPC: G01N1/34

CPC classification number: G01N1/34 ,  B01L3/50273 ,  C12M23/16 ,  C12M47/12 ,  C12N15/1003 ,  G01N27/447 ,  G01N30/24

Abstract: Embodiments of the present techniques provide systems and methods for isolating particular classes of biological molecules, for example, proteins or nucleic acids, from mixtures of biological components. The methods use solutions that react with the biological molecules to enhance their adsorption by substrates, allowing contaminants to be washed away from the targeted molecules. Embodiments include automated systems that can be used to implement the technique with no or minimal intervention. Other embodiments include separation column technologies that may be used in the techniques.

Abstract translation: 本技术的实施方案提供了从生物组分的混合物中分离特定类别的生物分子例如蛋白质或核酸的系统和方法。 该方法使用与生物分子反应的溶液，以增强其对底物的吸附，从而使污染物从目标分子中被冲走。 实施例包括可用于实现没有或最小干预的技术的自动化系统。 其他实施方案包括可用于该技术的分离塔技术。

公开(公告)号：US20140093878A1

公开(公告)日：2014-04-03

申请号：US13840062

申请日：2013-03-15

Applicant: General Electric Company

Inventor： John Richard Nelson ,  Robert Scott Duthie ,  Gregory Andrew Grossman ,  Anuradha Sekher

IPC: C12Q1/68 ,  C12N9/22

CPC classification number: C12N9/22 ,  C07K1/00 ,  C07K14/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6858 ,  C12Y301/26 ,  C12Q2521/301 ,  C12Q2525/101 ,  C12Q2537/1376 ,  C12Q2527/101

Abstract: Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.

Abstract translation: 本文提供了能够切割含有肌苷的DNA序列的突变体内切核酸酶V酶。 还描述了使用这种突变体内切核酸酶V酶将切口引入到包括一种或多种肌苷的靶DNA中并使用DNA聚合酶产生靶DNA扩增子的核酸测定法和试剂。

公开(公告)号：US10472620B2

公开(公告)日：2019-11-12

申请号：US14321160

申请日：2014-07-01

Applicant: General Electric Company

Inventor： John Richard Nelson ,  David Roger Moore ,  Robert Scott Duthie ,  Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Elizabeth Marie Dees ,  Patrick McCoy Spooner ,  Erik Leeming Kvam ,  Andrew Arthur Paul Burns ,  Vicki Herzl Watkins

IPC: C12P19/34 ,  C12N15/10

Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.

公开(公告)号：US20190071704A1

公开(公告)日：2019-03-07

申请号：US16041734

申请日：2018-07-20

Applicant: GENERAL ELECTRIC COMPANY

Inventor： John Richard Nelson ,  Robert Scott Duthie ,  Erik Leeming Kvam ,  Wei Gao

IPC: C12P21/00 ,  C12N15/67 ,  C12N15/63

Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.

公开(公告)号：US10100292B2

公开(公告)日：2018-10-16

申请号：US15048624

申请日：2016-02-19

Applicant: General Electric Company

Inventor： John Richard Nelson ,  Robert Scott Duthie ,  Gregory Andrew Grossman ,  Anuradha Sekher

IPC: C12Q1/68 ,  C12N9/22 ,  C12Q1/6858 ,  C07K14/00 ,  C07K1/00 ,  C12Q1/6844 ,  C12Q1/6806

Abstract: Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.

公开(公告)号：US09506847B2

公开(公告)日：2016-11-29

申请号：US14285099

申请日：2014-05-22

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Robert Scott Duthie ,  Wei-Cheng Tian ,  Tarun Khurana

IPC: G01N1/34 ,  C12M3/06 ,  C12M1/00 ,  C12N15/10 ,  B01L3/00 ,  G01N27/447 ,  G01N30/24

CPC classification number: G01N1/34 ,  B01L3/50273 ,  C12M23/16 ,  C12M47/12 ,  C12N15/1003 ,  G01N27/447 ,  G01N30/24

Abstract: Embodiments of the present techniques provide systems and methods for isolating particular classes of biological molecules, for example, proteins or nucleic acids, from mixtures of biological components. The methods use solutions that react with the biological molecules to enhance their adsorption by substrates, allowing contaminants to be washed away from the targeted molecules. Embodiments include automated systems that can be used to implement the technique with no or minimal intervention. Other embodiments include separation column technologies that may be used in the techniques.

Abstract translation: 本技术的实施方案提供了从生物组分的混合物中分离特定类别的生物分子例如蛋白质或核酸的系统和方法。 该方法使用与生物分子反应的溶液，以增强其对底物的吸附，从而使污染物从目标分子中被冲走。 实施例包括可用于实现没有或最小干预的技术的自动化系统。 其他实施方案包括可用于该技术的分离塔技术。